// Import generic module functions include { initOptions; saveFiles; getSoftwareName } from './functions' process STAR_ALIGN { tag "$meta.id" label 'process_high' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) } // Don't upgrade me - 2.7X indices incompatible with iGenomes. container "quay.io/biocontainers/star:2.6.1d--0" //container "https://depot.galaxyproject.org/singularity/star:2.6.1d--0" conda (params.conda ? "bioconda::star=2.6.1d" : null) input: tuple val(meta), path(reads) path index path gtf val options output: tuple val(meta), path("*Aligned.out.bam") , emit: bam tuple val(meta), path("*Log.final.out") , emit: log_final tuple val(meta), path("*Log.out") , emit: log_out tuple val(meta), path("*Log.progress.out"), emit: log_progress path "*.version.txt" , emit: version tuple val(meta), path("*sortedByCoord.out.bam") , optional:true, emit: bam_sorted tuple val(meta), path("*toTranscriptome.out.bam"), optional:true, emit: bam_transcript tuple val(meta), path("*fastq.gz") , optional:true, emit: fastq tuple val(meta), path("*.tab") , optional:true, emit: tab script: def software = getSoftwareName(task.process) def ioptions = initOptions(options) def prefix = ioptions.suffix ? "${meta.id}${ioptions.suffix}" : "${meta.id}" def ignore_gtf = params.star_ignore_sjdbgtf ? '' : "--sjdbGTFfile $gtf" def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix'" """ STAR \\ --genomeDir $index \\ --readFilesIn $reads \\ --runThreadN $task.cpus \\ --outFileNamePrefix $prefix. \\ $ignore_gtf \\ $seq_center \\ $ioptions.args if [ -f ${prefix}.Unmapped.out.mate1 ]; then mv ${prefix}.Unmapped.out.mate1 ${prefix}.unmapped_1.fastq gzip ${prefix}.unmapped_1.fastq fi if [ -f ${prefix}.Unmapped.out.mate2 ]; then mv ${prefix}.Unmapped.out.mate2 ${prefix}.unmapped_2.fastq gzip ${prefix}.unmapped_2.fastq fi STAR --version | sed -e "s/STAR_//g" > ${software}.version.txt """ }