#!/usr/bin/env nextflow nextflow.enable.dsl = 2 include { SAMTOOLS_FLAGSTAT } from '../../../software/samtools/flagstat/main.nf' addParams( options: [:] ) include { SAMTOOLS_IDXSTATS } from '../../../software/samtools/idxstats/main.nf' addParams( options: [:] ) include { SAMTOOLS_INDEX } from '../../../software/samtools/index/main.nf' addParams( options: [:] ) include { SAMTOOLS_SORT } from '../../../software/samtools/sort/main.nf' addParams( options: [:] ) include { SAMTOOLS_STATS } from '../../../software/samtools/stats/main.nf' addParams( options: [:] ) include { SAMTOOLS_VIEW } from '../../../software/samtools/view/main.nf' addParams( options: [:] ) include { SAMTOOLS_MPILEUP } from '../../../software/samtools/mpileup/main.nf' addParams( options: [:] ) workflow test_samtools_flagstat { def input = [] input = [ [ id:'test', single_end:false ], // meta map file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam", checkIfExists: true), file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam.bai", checkIfExists: true) ] SAMTOOLS_FLAGSTAT ( input ) } workflow test_samtools_idxstats { def input = [] input = [ [ id:'test', single_end:false ], // meta map file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam", checkIfExists: true), file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam.bai", checkIfExists: true) ] SAMTOOLS_IDXSTATS ( input ) } workflow test_samtools_index { def input = [] input = [ [ id:'test', single_end:false ], // meta map file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam", checkIfExists: true) ] SAMTOOLS_INDEX ( input ) } // FIXME Why is this passing it an already sorted bam? workflow test_samtools_sort { def input = [] input = [ [ id:'test', single_end:false ], // meta map file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam", checkIfExists: true) ] SAMTOOLS_SORT ( input ) } workflow test_samtools_stats { def input = [] input = [ [ id:'test', single_end:false ], // meta map file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam", checkIfExists: true), file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam.bai", checkIfExists: true) ] SAMTOOLS_STATS ( input ) } workflow test_samtools_view { def input = [] input = [ [ id:'test', single_end:false ], // meta map file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam", checkIfExists: true) ] SAMTOOLS_VIEW ( input ) } workflow test_samtools_mpileup { def input = [] def fasta = [] input = [ [ id:'test', single_end:false ], // meta map file("${launchDir}/tests/data/bam/test.paired_end.sorted.bam", checkIfExists: true) ] fasta = [ file("${launchDir}/tests/data/fasta/E_coli/NC_010473.fa", checkIfExists: true) ] SAMTOOLS_MPILEUP ( input, fasta ) }