#!/usr/bin/env nextflow nextflow.enable.dsl = 2 include { UNTAR } from '../../../modules/untar/main.nf' addParams( options: [:] ) include { SAMTOOLS_VIEW } from '../../../modules/samtools/view/main.nf' addParams( options: ['suffix': '.sam'] ) include { METAPHLAN3 } from '../../../modules/metaphlan3/main.nf' addParams( options: [ 'args':'--index mpa_v30_CHOCOPhlAn_201901 --add_viruses --bt2_ps very-sensitive-local' ] ) workflow test_metaphlan3_single_end { input = [ [ id:'test', single_end:true ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] ] db = channel.fromPath('https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/delete_me/metaphlan_database.tar.gz', type: 'dir', checkIfExists: true) UNTAR ( db ) METAPHLAN3 ( input, UNTAR.out.untar ) } workflow test_metaphlan3_paired_end { input = [ [ id:'test', single_end:false ], // meta map [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] ] db = channel.fromPath('https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/delete_me/metaphlan_database.tar.gz', type: 'dir', checkIfExists: true) UNTAR ( db ) METAPHLAN3 ( input, UNTAR.out.untar ) } workflow test_metaphlan3_sam { input = [ [ id:'test'], // meta map [ file(params.test_data['sarscov2']['illumina']['test_single_end_bam'], checkIfExists: true) ] ] db = channel.fromPath('https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/delete_me/metaphlan_database.tar.gz', type: 'dir', checkIfExists: true) UNTAR ( db ) SAMTOOLS_VIEW ( input ) METAPHLAN3 ( SAMTOOLS_VIEW.out.bam, UNTAR.out.untar ) } workflow test_metaphlan3_fasta { input = [ [ id:'test', single_end:true], // meta map [ file(params.test_data['sarscov2']['genome']['transcriptome_fasta'], checkIfExists: true) ] ] db = channel.fromPath('https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/delete_me/metaphlan_database.tar.gz', type: 'dir', checkIfExists: true) UNTAR ( db ) METAPHLAN3 ( input, UNTAR.out.untar ) }