// Import generic module functions include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions' params.options = [:] options = initOptions(params.options) process GATK4_APPLYBQSR { tag "$meta.id" label 'process_low' publishDir "${params.outdir}", mode: params.publish_dir_mode, saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) } conda (params.enable_conda ? "bioconda::gatk4=4.2.3.0" : null) if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { container "https://depot.galaxyproject.org/singularity/gatk4:4.2.3.0--hdfd78af_0" } else { container "quay.io/biocontainers/gatk4:4.2.3.0--hdfd78af_0" } input: tuple val(meta), path(input), path(input_index), path(bqsr_table) path fasta path fastaidx path dict path intervals output: tuple val(meta), path("*.bam"), emit: bam path "versions.yml" , emit: versions script: def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}" def interval = intervals ? "-L ${intervals}" : "" if (!task.memory) { log.info '[GATK ApplyBQSR] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = task.memory.giga } """ gatk ApplyBQSR \\ -R $fasta \\ -I $input \\ --bqsr-recal-file $bqsr_table \\ $interval \\ --tmp-dir . \\ -O ${prefix}.bam \\ $options.args cat <<-END_VERSIONS > versions.yml ${getProcessName(task.process)}: ${getSoftwareName(task.process)}: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//') END_VERSIONS """ }