process PICARD_FASTQTOSAM { tag "$meta.id" label 'process_medium' conda (params.enable_conda ? "bioconda::picard=2.27.4" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/picard:2.27.4--hdfd78af_0' : 'quay.io/biocontainers/picard:2.27.4--hdfd78af_0' }" input: tuple val(meta), path(reads) output: tuple val(meta), path("*.bam"), emit: bam path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" if (!task.memory) { log.warn '[Picard FastqToSam] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } def avail_mem = task.memory ? task.memory.giga : 3 def input = meta.single_end ? "--FASTQ ${reads}" : "--FASTQ ${reads[0]} --FASTQ2 ${reads[1]}" def sample_name = args.contains("--SAMPLE_NAME") || args.contains("-SM") ? "" : "--SAMPLE_NAME ${prefix}" """ picard \\ -Xmx${avail_mem}g \\ FastqToSam \\ ${args} \\ ${input} \\ ${sample_name} \\ --OUTPUT ${prefix}.bam cat <<-END_VERSIONS > versions.yml "${task.process}": picard: \$(picard FastqToSam --version 2>&1 | grep -o 'Version:.*' | cut -f2- -d:) END_VERSIONS """ }