name: artic_minion description: | Run the alignment/variant-call/consensus logic of the artic pipeline keywords: - artic - aggregate - demultiplexed reads tools: - artic: description: ARTIC pipeline - a bioinformatics pipeline for working with virus sequencing data sequenced with nanopore homepage: https://artic.readthedocs.io/en/latest/ documentation: https://artic.readthedocs.io/en/latest/ tool_dev_url: https://github.com/artic-network/fieldbioinformatics doi: "" licence: ['MIT'] input: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - fastq: type: file description: FastQ file containing reads pattern: "*.{fastq.gz}" - fast5_dir: type: directory description: Directory containing MinION FAST5 files pattern: "*" - sequencing_summary: type: file description: Path to Guppy sequencing summary pattern: "*.{txt}" - primer_scheme_fasta: type: file description: | Sequence of the reference genome used in the scheme in FASTA format pattern: "*.{reference.fasta}" - primer_scheme_bed: type: file description: | bed files containing coordinates of each primer in the scheme, relative to the reference genome pattern: "*.{scheme.bed}" - medaka_model: type: file description: | Medaka model to use (if option --medaka is set) pattern: "*.*" - scheme: type: value description: Name of the primer scheme - scheme_version: type: value description: Version of the primer scheme output: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - results: type: description: Aggregated FastQ files pattern: "*.fastq.gz" - bam: type: file description: BAM file pattern: "*.{sorted.bam}" - bai: type: file description: BAM index file pattern: "*.{sorted.bai}" - bam_trimmed: type: file description: BAM file with the primers left on pattern: "*.{trimmed.rg.sorted.bam}" - bai_trimmed: type: file description: BAM index file of bam_trimmed pattern: "*.{sorted.bai}" - bam_primertrimmed: type: file description: BAM containing reads after primer-binding site trimming pattern: "*.{trimmed.rg.sorted.bam}" - bai_primertrimmed: type: file description: BAM index file of bam_primertrimmed pattern: "*.{primertrimmed.rg.sorted.bam.bai}" - fasta: type: file description: FAST file with consensus sequence pattern: "*.{consensus.fasta}" - vcf: type: file description: VCF file containing detected variants passing quality filter pattern: "*.{pass.vcf.gz}" - tbi: type: file description: VCF index pattern: "*.{pass.vcf.gz.tbi}" - json: type: file description: JSON file for MultiQC pattern: "*.json" - versions: type: file description: File containing software versions pattern: "versions.yml" authors: - "@joseespinosa" - "@drpatelh"