process MOSDEPTH { tag "$meta.id" label 'process_medium' conda (params.enable_conda ? 'bioconda::mosdepth=0.3.3' : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mosdepth:0.3.3--hdfd78af_1' : 'quay.io/biocontainers/mosdepth:0.3.3--hdfd78af_1'}" input: tuple val(meta), path(bam), path(bai) path bed path fasta output: tuple val(meta), path('*.global.dist.txt') , emit: global_txt tuple val(meta), path('*.summary.txt') , emit: summary_txt tuple val(meta), path('*.region.dist.txt') , optional:true, emit: regions_txt tuple val(meta), path('*.per-base.d4') , optional:true, emit: per_base_d4 tuple val(meta), path('*.per-base.bed.gz') , optional:true, emit: per_base_bed tuple val(meta), path('*.per-base.bed.gz.csi') , optional:true, emit: per_base_csi tuple val(meta), path('*.regions.bed.gz') , optional:true, emit: regions_bed tuple val(meta), path('*.regions.bed.gz.csi') , optional:true, emit: regions_csi tuple val(meta), path('*.quantized.bed.gz') , optional:true, emit: quantized_bed tuple val(meta), path('*.quantized.bed.gz.csi') , optional:true, emit: quantized_csi tuple val(meta), path('*.thresholds.bed.gz') , optional:true, emit: thresholds_bed tuple val(meta), path('*.thresholds.bed.gz.csi'), optional:true, emit: thresholds_csi path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def reference = fasta ? "--fasta ${fasta}" : "" def interval = bed ? "--by ${bed}" : "" if (bed && args.contains("--by")) { exit 1, "'--by' can only be specified once when running mosdepth! Either remove input BED file definition or remove '--by' from 'ext.args' definition" } if (!bed && args.contains("--thresholds")) { exit 1, "'--thresholds' can only be specified in conjunction with '--by'" } """ mosdepth \\ --threads $task.cpus \\ $interval \\ $reference \\ $args \\ $prefix \\ $bam cat <<-END_VERSIONS > versions.yml "${task.process}": mosdepth: \$(mosdepth --version 2>&1 | sed 's/^.*mosdepth //; s/ .*\$//') END_VERSIONS """ stub: def prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}.global.dist.txt touch ${prefix}.region.dist.txt touch ${prefix}.summary.txt touch ${prefix}.per-base.d4 touch ${prefix}.per-base.bed.gz touch ${prefix}.per-base.bed.gz.csi touch ${prefix}.regions.bed.gz touch ${prefix}.regions.bed.gz.csi touch ${prefix}.quantized.bed.gz touch ${prefix}.quantized.bed.gz.csi touch ${prefix}.thresholds.bed.gz touch ${prefix}.thresholds.bed.gz.csi cat <<-END_VERSIONS > versions.yml "${task.process}": mosdepth: \$(mosdepth --version 2>&1 | sed 's/^.*mosdepth //; s/ .*\$//') END_VERSIONS """ }