process DRAGMAP_ALIGN { tag "$meta.id" label 'process_high' conda (params.enable_conda ? "bioconda::dragmap=1.2.1 bioconda::samtools=1.14 conda-forge::pigz=2.3.4" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-580d344d9d4a496cd403932da8765f9e0187774d:f7aad9060cde739c95685fc5ff6d6f7e3ec629c8-0': 'quay.io/biocontainers/mulled-v2-580d344d9d4a496cd403932da8765f9e0187774d:f7aad9060cde739c95685fc5ff6d6f7e3ec629c8-0' }" input: tuple val(meta), path(reads) path hashmap val sort_bam output: tuple val(meta), path("*.bam"), emit: bam tuple val(meta), path('*.log'), emit: log path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def read_group = meta.read_group ? "--RGSM ${meta.read_group}" : "" def samtools_command = sort_bam ? 'sort' : 'view' if (meta.single_end) { """ dragen-os \\ -r $hashmap \\ $args \\ $read_group \\ --num-threads $task.cpus \\ -1 $reads \\ 2> ${prefix}.dragmap.log \\ | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.bam - cat <<-END_VERSIONS > versions.yml "${task.process}": dragmap: \$(echo \$(dragen-os --version 2>&1)) samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) END_VERSIONS """ } else { """ dragen-os \\ -r $hashmap \\ $args \\ $read_group \\ --num-threads $task.cpus \\ -1 ${reads[0]} \\ -2 ${reads[1]} \\ 2> ${prefix}.dragmap.log \\ | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.bam - cat <<-END_VERSIONS > versions.yml "${task.process}": dragmap: \$(echo \$(dragen-os --version 2>&1)) samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) END_VERSIONS """ } }