nf-core_modules/modules/umitools/dedup/main.nf
Simon Pearce 9d489648a7
Set PYTHONHASHSEED for umitools dedup (#1932)
Set PYTHONHASHSEED

Co-authored-by: Simon Pearce <simon.pearce@cruk.manchester.ac.uk>
2022-08-31 14:05:12 +01:00

45 lines
1.5 KiB
Text

process UMITOOLS_DEDUP {
tag "$meta.id"
label "process_medium"
conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
input:
tuple val(meta), path(bam), path(bai)
val get_output_stats
output:
tuple val(meta), path("*.bam") , emit: bam
tuple val(meta), path("*edit_distance.tsv"), optional:true, emit: tsv_edit_distance
tuple val(meta), path("*per_umi.tsv") , optional:true, emit: tsv_per_umi
tuple val(meta), path("*per_position.tsv") , optional:true, emit: tsv_umi_per_position
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def paired = meta.single_end ? "" : "--paired"
def stats = get_output_stats ? "--output-stats $prefix" : ""
if (!(args ==~ /.*--random-seed.*/)) {args += " --random-seed=100"}
"""
PYTHONHASHSEED=0 umi_tools \\
dedup \\
-I $bam \\
-S ${prefix}.bam \\
$stats \\
$paired \\
$args
cat <<-END_VERSIONS > versions.yml
"${task.process}":
umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
END_VERSIONS
"""
}