nf-core_modules/modules/umitools/extract/main.nf
2022-02-04 09:53:32 +01:00

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process UMITOOLS_EXTRACT {
tag "$meta.id"
label "process_low"
conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
input:
tuple val(meta), path(reads)
output:
tuple val(meta), path("*.fastq.gz"), emit: reads
tuple val(meta), path("*.log") , emit: log
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
if (meta.single_end) {
"""
umi_tools \\
extract \\
-I $reads \\
-S ${prefix}.umi_extract.fastq.gz \\
$args \\
> ${prefix}.umi_extract.log
cat <<-END_VERSIONS > versions.yml
"${task.process}":
umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
END_VERSIONS
"""
} else {
"""
umi_tools \\
extract \\
-I ${reads[0]} \\
--read2-in=${reads[1]} \\
-S ${prefix}.umi_extract_1.fastq.gz \\
--read2-out=${prefix}.umi_extract_2.fastq.gz \\
$args \\
> ${prefix}.umi_extract.log
cat <<-END_VERSIONS > versions.yml
"${task.process}":
umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
END_VERSIONS
"""
}
}