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72 lines
2.9 KiB
Text
72 lines
2.9 KiB
Text
// Import generic module functions
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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params.options = [:]
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def options = initOptions(params.options)
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process FASTP {
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tag "$meta.id"
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
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conda (params.enable_conda ? 'bioconda::fastp=0.20.1=h8b12597_0' : null)
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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container 'https://depot.galaxyproject.org/singularity/fastp:0.20.1--h8b12597_0'
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} else {
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container 'quay.io/biocontainers/fastp:0.20.1--h8b12597_0'
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}
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input:
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tuple val(meta), path(reads)
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output:
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tuple val(meta), path('*.trim.fastq.gz'), emit: reads
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tuple val(meta), path('*.json') , emit: json
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tuple val(meta), path('*.html') , emit: html
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tuple val(meta), path('*.log') , emit: log
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path '*.version.txt' , emit: version
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tuple val(meta), path('*.fail.fastq.gz'), optional:true, emit: reads_fail
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script:
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// Added soft-links to original fastqs for consistent naming in MultiQC
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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if (meta.single_end) {
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def fail_fastq = params.save_trimmed_fail ? "--failed_out ${prefix}.fail.fastq.gz" : ''
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"""
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[ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
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fastp \\
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--in1 ${prefix}.fastq.gz \\
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--out1 ${prefix}.trim.fastq.gz \\
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--thread $task.cpus \\
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--json ${prefix}.fastp.json \\
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--html ${prefix}.fastp.html \\
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$fail_fastq \\
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$options.args \\
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2> ${prefix}.fastp.log
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echo \$(fastp --version 2>&1) | sed -e "s/fastp //g" > ${software}.version.txt
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"""
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} else {
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def fail_fastq = params.save_trimmed_fail ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : ''
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"""
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[ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
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[ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
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fastp \\
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--in1 ${prefix}_1.fastq.gz \\
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--in2 ${prefix}_2.fastq.gz \\
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--out1 ${prefix}_1.trim.fastq.gz \\
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--out2 ${prefix}_2.trim.fastq.gz \\
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--json ${prefix}.fastp.json \\
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--html ${prefix}.fastp.html \\
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$fail_fastq \\
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--thread $task.cpus \\
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--detect_adapter_for_pe \\
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$options.args \\
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2> ${prefix}.fastp.log
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echo \$(fastp --version 2>&1) | sed -e "s/fastp //g" > ${software}.version.txt
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"""
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}
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}
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