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38 lines
1.3 KiB
Text
38 lines
1.3 KiB
Text
process GATK4_SAMTOFASTQ {
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tag "$meta.id"
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label 'process_medium'
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conda (params.enable_conda ? "bioconda::gatk4=4.2.4.1" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/gatk4:4.2.4.1--hdfd78af_0' :
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'quay.io/biocontainers/gatk4:4.2.4.1--hdfd78af_0' }"
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input:
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tuple val(meta), path(bam)
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output:
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tuple val(meta), path('*.fastq.gz'), emit: fastq
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path "versions.yml" , emit: versions
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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def output = meta.single_end ? "FASTQ=${prefix}.fastq.gz" : "FASTQ=${prefix}_1.fastq.gz SECOND_END_FASTQ=${prefix}_2.fastq.gz"
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def avail_mem = 3
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if (!task.memory) {
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log.info '[GATK SamToFastq] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
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} else {
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avail_mem = task.memory.giga
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}
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"""
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gatk --java-options "-Xmx${avail_mem}g" SamToFastq \\
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I=$bam \\
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$output \\
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$args
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
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END_VERSIONS
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"""
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}
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