nf-core_modules/modules/metabat2/metabat2/main.nf
2022-02-04 09:53:32 +01:00

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process METABAT2_METABAT2 {
tag "$meta.id"
label 'process_medium'
conda (params.enable_conda ? "bioconda::metabat2=2.15" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/metabat2:2.15--h986a166_1' :
'quay.io/biocontainers/metabat2:2.15--h986a166_1' }"
input:
tuple val(meta), path(fasta), path(depth)
output:
tuple val(meta), path("*.tooShort.fa.gz") , optional:true , emit: tooshort
tuple val(meta), path("*.lowDepth.fa.gz") , optional:true , emit: lowdepth
tuple val(meta), path("*.unbinned.fa.gz") , optional:true , emit: unbinned
tuple val(meta), path("*.tsv.gz") , optional:true , emit: membership
tuple val(meta), path("bins/*.fa.gz") , optional:true , emit: fasta
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def decompress_depth = depth ? "gzip -d -f $depth" : ""
def depth_file = depth ? "-a ${depth.baseName}" : ""
"""
$decompress_depth
metabat2 \\
$args \\
-i $fasta \\
$depth_file \\
-t $task.cpus \\
--saveCls \\
-o metabat2/${prefix}
mv metabat2/${prefix} ${prefix}.tsv
mv metabat2 bins
gzip ${prefix}.tsv
find ./bins/ -name "*.fa" -type f | xargs -t -n 1 bgzip -@ ${task.cpus}
find ./bins/ -name "*[lowDepth,tooShort,unbinned].fa.gz" -type f -exec mv {} . \\;
cat <<-END_VERSIONS > versions.yml
"${task.process}":
metabat2: \$( metabat2 --help 2>&1 | head -n 2 | tail -n 1| sed 's/.*\\:\\([0-9]*\\.[0-9]*\\).*/\\1/' )
END_VERSIONS
"""
}