mirror of
https://github.com/MillironX/nf-core_modules.git
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9ae34a01d1
* Fix typo * Add stub runs for testing input without matched normals * Add missing -stub-run * remove empty file checksum tests and change workflow names * test controlfreec naming * fix output file names * fix output file names * fix output file names * fix conda and container path difference for R scripts * update tar version to work with conda * fix version number in docker * try to fix path to script, pretty sure it won't work * try new ways to set path with wildcard * try which * add which but with escape * remove comment
176 lines
13 KiB
Text
176 lines
13 KiB
Text
process CONTROLFREEC_FREEC {
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tag "$meta.id"
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label 'process_low'
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conda (params.enable_conda ? "bioconda::control-freec=11.6" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/control-freec:11.6--h1b792b2_1':
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'quay.io/biocontainers/control-freec:11.6--h1b792b2_1' }"
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input:
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tuple val(meta), path(mpileup_normal), path(mpileup_tumor), path(cpn_normal), path(cpn_tumor), path(minipileup_normal), path(minipileup_tumor)
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path fasta
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path fai
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path snp_position
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path known_snps
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path known_snps_tbi
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path chr_directory
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path mappability
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path target_bed
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path gccontent_profile
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output:
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tuple val(meta), path("*_ratio.BedGraph") , emit: bedgraph, optional: true
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tuple val(meta), path("*_control.cpn") , emit: control_cpn, optional: true
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tuple val(meta), path("*_sample.cpn") , emit: sample_cpn
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tuple val(meta), path("GC_profile.*.cpn") , emit: gcprofile_cpn, optional:true
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tuple val(meta), path("*_BAF.txt") , emit: BAF
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tuple val(meta), path("*_CNVs") , emit: CNV
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tuple val(meta), path("*_info.txt") , emit: info
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tuple val(meta), path("*_ratio.txt") , emit: ratio
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tuple val(meta), path("config.txt") , emit: config
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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//"General" configurations
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def bedgraphoutput = task.ext.args?["general"]?["bedgraphoutput"] ? "BedGraphOutput = ${task.ext.args["general"]["bedgraphoutput"]}" : ""
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def chr_files = chr_directory ? "chrFiles =\${PWD}/${chr_directory}" : ""
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def chr_length = fai ? "chrLenFile = \${PWD}/${fai}" : ""
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def breakpointthreshold = task.ext.args?["general"]?["breakpointthreshold"] ? "breakPointThreshold = ${task.ext.args["general"]["breakpointthreshold"]}" : ""
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def breakpointtype = task.ext.args?["general"]?["breakpointtype"] ? "breakPointType = ${task.ext.args["general"]["breakpointtype"]}" : ""
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def coefficientofvariation = task.ext.args?["general"]?["coefficientofvariation"] ? "coefficientOfVariation = ${task.ext.args["general"]["coefficientofvariation"]}" : ""
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def contamination = task.ext.args?["general"]?["contamination"] ? "contamination = ${task.ext.args["general"]["contamination"]}" : ""
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def contaminationadjustment = task.ext.args?["general"]?["contaminationadjustment"] ? "contaminationAdjustment = ${task.ext.args["general"]["contaminationadjustment"]}" : ""
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def degree = task.ext.args?["general"]?["degree"] ? "degree = ${task.ext.args["general"]["degree"]}" : ""
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def forcegccontentnormalization = task.ext.args?["general"]?["forcegccontentnormalization"] ? "forceGCcontentNormalization = ${task.ext.args["general"]["forcegccontentnormalization"]}" : ""
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def gccontentprofile = gccontent_profile ? "GCcontentProfile = ${gccontent_profile}" : ""
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def mappability = mappability ? "gemMappabilityFile = \${PWD}/${mappability}" : ""
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def intercept = task.ext.args?["general"]?["intercept"] ? "intercept = ${task.ext.args["general"]["intercept"]}" : ""
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def mincnalength = task.ext.args?["general"]?["mincnalength"] ? "minCNAlength = ${task.ext.args["general"]["mincnalength"]}" : ""
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def minmappabilityperwindow = task.ext.args?["general"]?["minmappabilityperwindow"] ? "minMappabilityPerWindow = ${task.ext.args["general"]["minmappabilityperwindow"]}" : ""
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def minexpectedgc = task.ext.args?["general"]?["minexpectedgc"] ? "minExpectedGC = ${task.ext.args["general"]["minexpectedgc"]}" : ""
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def maxexpectedgc = task.ext.args?["general"]?["maxexpectedgc"] ? "maxExpectedGC = ${task.ext.args["general"]["maxexpectedgc"]}" : ""
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def minimalsubclonepresence = task.ext.args?["general"]?["minimalsubclonepresence"] ? "minimalSubclonePresence = ${task.ext.args["general"]["minimalsubclonepresence"]}" : ""
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def noisydata = task.ext.args?["general"]?["noisydata"] ? "noisyData = ${task.ext.args["general"]["noisydata"]}" : ""
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def output = task.ext.prefix ? "outputDir = \${PWD}/${task.ext.prefix}" : ""
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def ploidy = task.ext.args?["general"]?["ploidy"] ? "ploidy = ${task.ext.args["general"]["ploidy"]}" : ""
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def printNA = task.ext.args?["general"]?["printNA"] ? "printNA = ${task.ext.args["general"]["printNA"]}" : ""
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def readcountthreshold = task.ext.args?["general"]?["readcountthreshold"] ? "readCountThreshold = ${task.ext.args["general"]["readcountthreshold"]}" : ""
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def sex = task.ext.args?["general"]?["sex"] ? "sex = ${task.ext.args["general"]["sex"]}" : ""
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def step = task.ext.args?["general"]?["step"] ? "step = ${task.ext.args["general"]["step"]}" : ""
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def telocentromeric = task.ext.args?["general"]?["telocentromeric"] ? "telocentromeric = ${task.ext.args["general"]["telocentromeric"]} " : ""
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def uniquematch = task.ext.args?["general"]?["uniquematch"] ? "uniqueMatch = ${task.ext.args["general"]["uniquematch"]}" : ""
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def window = task.ext.args?["general"]?["window"] ? "window = ${task.ext.args["general"]["window"]}" : ""
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//"Control" configurations
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def matefile_normal = mpileup_normal ? "mateFile = \${PWD}/${mpileup_normal}" : ""
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def matecopynumberfile_normal = cpn_normal ? "mateCopyNumberFile = \${PWD}/${cpn_normal}" : ""
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def minipileup_normal = minipileup_normal ? "miniPileup = \${PWD}/${minipileup_normal}" : ""
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def inputformat_normal = task.ext.args?["control"]?["inputformat"] ? "inputFormat = ${task.ext.args["control"]["inputformat"]}" : ""
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def mateorientation_normal = task.ext.args?["control"]?["mateorientation"] ? "mateOrientation = ${task.ext.args["control"]["mateorientation"]}" : ""
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//"Sample" configuration
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def matefile_tumor = mpileup_tumor ? "mateFile = \${PWD}/${mpileup_tumor}" : ""
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def matecopynumberfile_tumor = cpn_tumor ? "mateCopyNumberFile = \${PWD}/${cpn_tumor}" : ""
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def minipileup_tumor = minipileup_tumor ? "miniPileup = \${PWD}/${minipileup_tumor}" : ""
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def inputformat_tumor = task.ext.args?["sample"]?["inputformat"] ? "inputFormat = ${task.ext.args["sample"]["inputformat"]}" : ""
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def mateorientation_tumor = task.ext.args?["sample"]?["mateorientation"] ? "mateOrientation = ${task.ext.args["sample"]["mateorientation"]}" : ""
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//"BAF" configuration
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def makepileup = snp_position ? "makePileup = \${PWD}/${snp_position}" : ""
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def fastafile = fasta ? "fastaFile = \${PWD}/${fasta}" : ""
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def minimalcoverageperposition = task.ext.args?["BAF"]?["minimalcoverageperposition"] ? "minimalCoveragePerPosition = ${task.ext.args["BAF"]["minimalcoverageperposition"]}" : ""
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def minimalqualityperposition = task.ext.args?["BAF"]?["minimalqualityperposition"] ? "minimalQualityPerPosition = ${task.ext.args["BAF"]["minimalqualityperposition"]}" : ""
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def shiftinquality = task.ext.args?["BAF"]?["shiftinquality"] ? "shiftInQuality = ${task.ext.args["BAF"]["shiftinquality"]}" : ""
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def snpfile = known_snps ? "SNPfile = \$PWD/${known_snps}" : ""
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//"Target" configuration
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def target_bed = target_bed ? "captureRegions = ${target_bed}" : ""
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"""
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touch config.txt
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echo "[general]" >> config.txt
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echo ${bedgraphoutput} >> config.txt
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echo ${breakpointthreshold} >> config.txt
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echo ${breakpointtype} >> config.txt
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echo ${chr_files} >> config.txt
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echo ${chr_length} >> config.txt
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echo ${coefficientofvariation} >> config.txt
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echo ${contamination} >> config.txt
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echo ${contaminationadjustment} >> config.txt
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echo ${degree} >> config.txt
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echo ${forcegccontentnormalization} >> config.txt
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echo ${gccontentprofile} >> config.txt
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echo ${mappability} >> config.txt
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echo ${intercept} >> config.txt
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echo ${mincnalength} >> config.txt
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echo ${minmappabilityperwindow} >> config.txt
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echo ${minexpectedgc} >> config.txt
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echo ${maxexpectedgc} >> config.txt
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echo ${minimalsubclonepresence} >> config.txt
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echo "maxThreads = ${task.cpus}" >> config.txt
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echo ${noisydata} >> config.txt
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echo ${output} >> config.txt
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echo ${ploidy} >> config.txt
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echo ${printNA} >> config.txt
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echo ${readcountthreshold} >> config.txt
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echo ${sex} >> config.txt
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echo ${step} >> config.txt
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echo ${telocentromeric} >> config.txt
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echo ${uniquematch} >> config.txt
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echo ${window} >> config.txt
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echo "[control]" >> config.txt
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echo ${matefile_normal} >> config.txt
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echo ${matecopynumberfile_normal} >> config.txt
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echo ${minipileup_normal} >> config.txt
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echo ${inputformat_normal} >> config.txt
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echo ${mateorientation_normal} >> config.txt
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echo "[sample]" >> config.txt
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echo ${matefile_tumor} >> config.txt
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echo ${matecopynumberfile_tumor} >> config.txt
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echo ${minipileup_tumor} >> config.txt
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echo ${inputformat_tumor} >> config.txt
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echo ${mateorientation_tumor} >> config.txt
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echo "[BAF]" >> config.txt
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echo ${makepileup} >> config.txt
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echo ${fastafile} >> config.txt
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echo ${minimalcoverageperposition} >> config.txt
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echo ${minimalqualityperposition} >> config.txt
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echo ${shiftinquality} >> config.txt
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echo ${snpfile} >> config.txt
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echo "[target]" >> config.txt
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echo ${target_bed} >> config.txt
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freec -conf config.txt
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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controlfreec: \$(echo \$(freec -version 2>&1) | sed 's/^.*Control-FREEC //; s/:.*\$//' | sed -e "s/Control-FREEC v//g" )
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END_VERSIONS
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"""
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stub:
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def prefix = task.ext.prefix ?: "${meta.id}"
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"""
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touch ${prefix}_ratio.BedGraph
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touch ${prefix}_sample.cpn
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touch GC_profile.${prefix}.cpn
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touch ${prefix}_BAF.txt
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touch ${prefix}_CNVs
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touch ${prefix}_info.txt
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touch ${prefix}_ratio.txt
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touch config.txt
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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controlfreec: \$(echo \$(freec -version 2>&1) | sed 's/^.*Control-FREEC //; s/:.*\$//' | sed -e "s/Control-FREEC v//g" )
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END_VERSIONS
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"""
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}
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