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49 lines
1.6 KiB
Text
49 lines
1.6 KiB
Text
// Import generic module functions
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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process SUBREAD_FEATURECOUNTS {
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tag "$meta.id"
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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saveAs: { filename -> saveFiles(filename:filename, options:options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
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container "quay.io/biocontainers/subread:2.0.1--hed695b0_0"
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//container "https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0"
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conda (params.conda ? "bioconda::subread=2.0.1" : null)
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input:
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tuple val(meta), path(bams), path(annotation)
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val options
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output:
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tuple val(meta), path("*featureCounts.txt") , emit: counts
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tuple val(meta), path("*featureCounts.txt.summary"), emit: summary
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path "*.version.txt" , emit: version
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script:
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def software = getSoftwareName(task.process)
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def ioptions = initOptions(options)
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def prefix = ioptions.suffix ? "${meta.id}${ioptions.suffix}" : "${meta.id}"
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def paired_end = meta.single_end ? '' : '-p'
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def strandedness = 0
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if (meta.strandedness == 'forward') {
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strandedness = 1
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} else if (meta.strandedness == 'reverse') {
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strandedness = 2
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}
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"""
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featureCounts \\
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$ioptions.args \\
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$paired_end \\
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-T $task.cpus \\
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-a $annotation \\
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-s $strandedness \\
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-o ${prefix}.featureCounts.txt \\
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${bams.join(' ')}
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echo \$(featureCounts -v 2>&1) | sed -e "s/featureCounts v//g" > ${software}.version.txt
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"""
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}
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