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4398056204
* Add tests and yml file for macs2/callpeak * add format option for macs2 * update macs2/callpeak to accept format argument * update test.yml * update the container version. * try to fix the issue in conda container. * Update conda and containers * Going back to previous container versions Co-authored-by: JoseEspinosa <kadomu@gmail.com>
60 lines
2.1 KiB
Text
60 lines
2.1 KiB
Text
// Import generic module functions
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include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
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params.options = [:]
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options = initOptions(params.options)
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process MACS2_CALLPEAK {
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tag "$meta.id"
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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conda (params.enable_conda ? "bioconda::macs2=2.2.7.1" : null)
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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container "https://depot.galaxyproject.org/singularity/macs2:2.2.7.1--py38h4a8c8d9_3"
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} else {
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container "quay.io/biocontainers/macs2:2.2.7.1--py38h4a8c8d9_3"
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}
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input:
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tuple val(meta), path(ipbam), path(controlbam)
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val macs2_gsize
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output:
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tuple val(meta), path("*.{narrowPeak,broadPeak}"), emit: peak
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tuple val(meta), path("*.xls") , emit: xls
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path "versions.yml" , emit: versions
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tuple val(meta), path("*.gappedPeak"), optional:true, emit: gapped
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tuple val(meta), path("*.bed") , optional:true, emit: bed
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tuple val(meta), path("*.bdg") , optional:true, emit: bdg
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script:
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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def args = options.args.tokenize()
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def format = meta.single_end ? 'BAM' : 'BAMPE'
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def control = controlbam ? "--control $controlbam" : ''
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if(args.contains('--format')){
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def id = args.findIndexOf{it=='--format'}
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format = args[id+1]
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args.remove(id+1)
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args.remove(id)
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}
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"""
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macs2 \\
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callpeak \\
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${args.join(' ')} \\
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--gsize $macs2_gsize \\
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--format $format \\
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--name $prefix \\
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--treatment $ipbam \\
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$control
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cat <<-END_VERSIONS > versions.yml
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${getProcessName(task.process)}:
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${getSoftwareName(task.process)}: \$(macs2 --version | sed -e "s/macs2 //g")
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END_VERSIONS
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"""
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}
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