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The `last/lastal` submodule takes query sequences to align to a target index, and optionally takes one set of alignment parameters (including a score matrix) computed by the `last/train` module for each of the sequences. In the previous implementation the sequences and the alignment parameters were provided in different channels, causing them to be sometimes desynchronised. In the patched implementation, `last/lastal` takes a 3-tuple as input to ensure synchronicity. To produce this tuple in a pipeline, one can use the `join` command as in the following example. LAST_TRAIN ( query, target ) LAST_LASTAL ( query.join(LAST_TRAIN.out.param_file), target ) In case no parameter file is computed one can pass a dummy file to the module as follows: LAST_LASTAL ( query.map { row -> [ row[0], row[1], [] ] }, target ) |
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