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f4c69bc427
* bclconvert: initial commit * add most of tool * attempt at adding testing stub * add dockerfile + instructions * add container to module * update readme * more attempts at making stubs work * finish stub run * fix ci issues * more fixes to stub * add read version check to stub * fix some tests * update readme * fix version number * syntax fix * revert edit to output directory * Update modules/bclconvert/meta.yml Co-authored-by: James A. Fellows Yates <jfy133@gmail.com> * Update modules/bclconvert/meta.yml Co-authored-by: James A. Fellows Yates <jfy133@gmail.com> * update meta.yaml * update thread usage * Update modules/bclconvert/main.nf Co-authored-by: Edmund Miller <edmund.a.miller@protonmail.com> * Escape env variable * Update modules/bclconvert/Dockerfile Co-authored-by: Mark Whelan <7407040+MrMarkW@users.noreply.github.com> * fix comments by @Emiller88 * fix task.cpus Co-authored-by: James A. Fellows Yates <jfy133@gmail.com> Co-authored-by: Edmund Miller <edmund.a.miller@protonmail.com> Co-authored-by: Mark Whelan <7407040+MrMarkW@users.noreply.github.com>
81 lines
2.6 KiB
Text
81 lines
2.6 KiB
Text
process BCLCONVERT {
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tag '$samplesheet'
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label 'process_high'
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if (params.enable_conda) {
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exit 1, "Conda environments cannot be used when using bcl-convert. Please use docker or singularity containers."
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}
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container "nfcore/bclconvert:3.9.3"
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input:
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path samplesheet
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path run_dir
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output:
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path "*.fastq.gz" ,emit: fastq
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path "Reports/*.{csv,xml,bin}" ,emit: reports
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path "Logs/*.{log,txt}" ,emit: logs
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path "InterOp/*.bin" ,emit: interop
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path "versions.yml" ,emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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"""
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bcl-convert \
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$args \\
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--output-directory . \\
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--bcl-input-directory ${run_dir} \\
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--sample-sheet ${samplesheet} \\
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--bcl-num-parallel-tiles ${task.cpus}
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mkdir InterOp
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cp ${run_dir}/InterOp/*.bin InterOp/
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mv Reports/*.bin InterOp/
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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bclconvert: \$(bcl-convert -V 2>&1 | head -n 1 | sed 's/^.*Version //')
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END_VERSIONS
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"""
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stub:
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"""
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echo "sample1_S1_L001_R1_001" > sample1_S1_L001_R1_001.fastq.gz
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echo "sample1_S1_L001_R2_001" > sample1_S1_L001_R2_001.fastq.gz
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echo "sample1_S1_L002_R1_001" > sample1_S1_L002_R1_001.fastq.gz
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echo "sample1_S1_L002_R2_001" > sample1_S1_L002_R2_001.fastq.gz
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echo "sample2_S2_L001_R1_001" > sample2_S2_L001_R1_001.fastq.gz
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echo "sample2_S2_L001_R2_001" > sample2_S2_L001_R2_001.fastq.gz
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echo "sample2_S2_L002_R1_001" > sample2_S2_L002_R1_001.fastq.gz
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echo "sample2_S2_L002_R2_001" > sample2_S2_L002_R2_001.fastq.gz
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mkdir Reports
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echo "Adapter_Metrics" > Reports/Adapter_Metrics.csv
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echo "Demultiplex_Stats" > Reports/Demultiplex_Stats.csv
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echo "fastq_list" > Reports/fastq_list.csv
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echo "Index_Hopping_Counts" > Reports/Index_Hopping_Counts.csv
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echo "IndexMetricsOut" > Reports/IndexMetricsOut.bin
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echo "Quality_Metrics" > Reports/Quality_Metrics.csv
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echo "RunInfo" > Reports/RunInfo.xml
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echo "SampleSheet" > Reports/SampleSheet.csv
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echo "Top_Unknown_Barcodes" > Reports/Top_Unknown_Barcodes.csv
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mkdir Logs
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echo "Errors" > Logs/Errors.log
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echo "FastqComplete" > Logs/FastqComplete.txt
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echo "Info" > Logs/Info.log
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echo "Warnings" > Logs/Warnings.log
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mkdir InterOp/
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echo "InterOp" > InterOp/InterOp.bin
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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bclconvert: \$(bcl-convert -V 2>&1 | head -n 1 | sed 's/^.*Version //')
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END_VERSIONS
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"""
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}
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