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91 lines
3.6 KiB
Text
91 lines
3.6 KiB
Text
// Import generic module functions
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include { initOptions; saveFiles; getSoftwareName; getProcessName } from './functions'
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params.options = [:]
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options = initOptions(params.options)
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process TRIMGALORE {
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tag "$meta.id"
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label 'process_high'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
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conda (params.enable_conda ? 'bioconda::trim-galore=0.6.7' : null)
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if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
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container "https://depot.galaxyproject.org/singularity/trim-galore:0.6.7--hdfd78af_0"
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} else {
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container "quay.io/biocontainers/trim-galore:0.6.7--hdfd78af_0"
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}
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input:
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tuple val(meta), path(reads)
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output:
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tuple val(meta), path("*.fq.gz") , emit: reads
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tuple val(meta), path("*report.txt"), emit: log
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path "versions.yml" , emit: version
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tuple val(meta), path("*.html"), emit: html optional true
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tuple val(meta), path("*.zip") , emit: zip optional true
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script:
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// Calculate number of --cores for TrimGalore based on value of task.cpus
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// See: https://github.com/FelixKrueger/TrimGalore/blob/master/Changelog.md#version-060-release-on-1-mar-2019
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// See: https://github.com/nf-core/atacseq/pull/65
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def cores = 1
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if (task.cpus) {
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cores = (task.cpus as int) - 4
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if (meta.single_end) cores = (task.cpus as int) - 3
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if (cores < 1) cores = 1
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if (cores > 4) cores = 4
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}
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// Clipping presets have to be evaluated in the context of SE/PE
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def c_r1 = params.clip_r1 > 0 ? "--clip_r1 ${params.clip_r1}" : ''
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def c_r2 = params.clip_r2 > 0 ? "--clip_r2 ${params.clip_r2}" : ''
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def tpc_r1 = params.three_prime_clip_r1 > 0 ? "--three_prime_clip_r1 ${params.three_prime_clip_r1}" : ''
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def tpc_r2 = params.three_prime_clip_r2 > 0 ? "--three_prime_clip_r2 ${params.three_prime_clip_r2}" : ''
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// Added soft-links to original fastqs for consistent naming in MultiQC
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def software = getSoftwareName(task.process)
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def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
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if (meta.single_end) {
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"""
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[ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
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trim_galore \\
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$options.args \\
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--cores $cores \\
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--gzip \\
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$c_r1 \\
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$tpc_r1 \\
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${prefix}.fastq.gz
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cat <<-END_VERSIONS > versions.yml
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${getProcessName(task.process)}:
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${getSoftwareName(task.process)}: \$(echo \$(trim_galore --version 2>&1) | sed 's/^.*version //; s/Last.*\$//')
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cutadapt: \$(cutadapt --version)
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END_VERSIONS
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"""
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} else {
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"""
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[ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
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[ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
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trim_galore \\
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$options.args \\
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--cores $cores \\
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--paired \\
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--gzip \\
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$c_r1 \\
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$c_r2 \\
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$tpc_r1 \\
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$tpc_r2 \\
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${prefix}_1.fastq.gz \\
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${prefix}_2.fastq.gz
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cat <<-END_VERSIONS > versions.yml
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${getProcessName(task.process)}:
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${getSoftwareName(task.process)}: \$(echo \$(trim_galore --version 2>&1) | sed 's/^.*version //; s/Last.*\$//')
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cutadapt: \$(cutadapt --version)
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END_VERSIONS
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"""
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}
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}
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