nf-core_modules/modules/macs2/callpeak/main.nf
2022-02-04 09:53:32 +01:00

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process MACS2_CALLPEAK {
tag "$meta.id"
label 'process_medium'
conda (params.enable_conda ? "bioconda::macs2=2.2.7.1" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/macs2:2.2.7.1--py38h4a8c8d9_3' :
'quay.io/biocontainers/macs2:2.2.7.1--py38h4a8c8d9_3' }"
input:
tuple val(meta), path(ipbam), path(controlbam)
val macs2_gsize
output:
tuple val(meta), path("*.{narrowPeak,broadPeak}"), emit: peak
tuple val(meta), path("*.xls") , emit: xls
path "versions.yml" , emit: versions
tuple val(meta), path("*.gappedPeak"), optional:true, emit: gapped
tuple val(meta), path("*.bed") , optional:true, emit: bed
tuple val(meta), path("*.bdg") , optional:true, emit: bdg
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def args_list = args.tokenize()
def format = meta.single_end ? 'BAM' : 'BAMPE'
def control = controlbam ? "--control $controlbam" : ''
if(args_list.contains('--format')){
def id = args_list.findIndexOf{it=='--format'}
format = args_list[id+1]
args_list.remove(id+1)
args_list.remove(id)
}
"""
macs2 \\
callpeak \\
${args_list.join(' ')} \\
--gsize $macs2_gsize \\
--format $format \\
--name $prefix \\
--treatment $ipbam \\
$control
cat <<-END_VERSIONS > versions.yml
"${task.process}":
macs2: \$(macs2 --version | sed -e "s/macs2 //g")
END_VERSIONS
"""
}