nf-core_modules/software/salmon/quant/main.nf
2021-02-03 09:20:34 +00:00

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// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
params.options = [:]
def options = initOptions(params.options)
process SALMON_QUANT {
tag "$meta.id"
label "process_medium"
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
conda (params.enable_conda ? "bioconda::salmon=1.4.0=hf69c8f4_0" : null)
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/salmon:1.4.0--hf69c8f4_0"
} else {
container "quay.io/biocontainers/salmon:1.4.0--hf69c8f4_0"
}
input:
tuple val(meta), path(reads)
path index
path gtf
path transcript_fasta
val alignment_mode
output:
tuple val(meta), path("${prefix}"), emit: results
path "*.version.txt" , emit: version
script:
def software = getSoftwareName(task.process)
prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
def reference = "--index $index"
def input_reads = meta.single_end ? "-r $reads" : "-1 ${reads[0]} -2 ${reads[1]}"
if (alignment_mode) {
reference = "-t $transcript_fasta"
input_reads = "-a $reads"
}
def strandedness = meta.single_end ? 'U' : 'IU'
if (meta.strandedness == 'forward') {
strandedness = meta.single_end ? 'SF' : 'ISF'
} else if (meta.strandedness == 'reverse') {
strandedness = meta.single_end ? 'SR' : 'ISR'
}
"""
salmon quant \\
--geneMap $gtf \\
--threads $task.cpus \\
--libType=$strandedness \\
$reference \\
$input_reads \\
$options.args \\
-o $prefix
salmon --version | sed -e "s/salmon //g" > ${software}.version.txt
"""
}