mirror of
https://github.com/MillironX/nf-core_modules.git
synced 2024-12-22 11:08:17 +00:00
58 lines
1.9 KiB
Text
58 lines
1.9 KiB
Text
process GATK4_CNNSCOREVARIANTS {
|
|
tag "$meta.id"
|
|
label 'process_low'
|
|
|
|
//Conda is not supported at the moment: https://github.com/broadinstitute/gatk/issues/7811
|
|
if (params.enable_conda) {
|
|
exit 1, "Conda environments cannot be used for GATK4/CNNScoreVariants at the moment. Please use docker or singularity containers."
|
|
}
|
|
container 'broadinstitute/gatk:4.2.6.1' //Biocontainers is missing a package
|
|
|
|
input:
|
|
tuple val(meta), path(vcf), path(tbi), path(aligned_input), path(intervals)
|
|
path fasta
|
|
path fai
|
|
path dict
|
|
path architecture
|
|
path weights
|
|
|
|
output:
|
|
tuple val(meta), path("*cnn.vcf.gz") , emit: vcf
|
|
tuple val(meta), path("*cnn.vcf.gz.tbi"), emit: tbi
|
|
path "versions.yml" , emit: versions
|
|
|
|
when:
|
|
task.ext.when == null || task.ext.when
|
|
|
|
script:
|
|
def args = task.ext.args ?: ''
|
|
def prefix = task.ext.prefix ?: "${meta.id}"
|
|
def aligned_input = aligned_input ? "--input $aligned_input" : ""
|
|
def interval_command = intervals ? "--intervals $intervals" : ""
|
|
def architecture = architecture ? "--architecture $architecture" : ""
|
|
def weights = weights ? "--weights $weights" : ""
|
|
|
|
def avail_mem = 3
|
|
if (!task.memory) {
|
|
log.info '[GATK CnnScoreVariants] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
|
|
} else {
|
|
avail_mem = task.memory.giga
|
|
}
|
|
"""
|
|
gatk --java-options "-Xmx${avail_mem}g" CNNScoreVariants \\
|
|
--variant $vcf \\
|
|
--output ${prefix}.cnn.vcf.gz \\
|
|
--reference $fasta \\
|
|
$interval_command \\
|
|
$aligned_input \\
|
|
$architecture \\
|
|
$weights \\
|
|
--tmp-dir . \\
|
|
$args
|
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
"${task.process}":
|
|
gatk4: \$(echo \$(gatk --version 2>&1) | sed 's/^.*(GATK) v//; s/ .*\$//')
|
|
END_VERSIONS
|
|
"""
|
|
}
|