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86 lines
6 KiB
Text
86 lines
6 KiB
Text
// Import generic module functions
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include { initOptions; saveFiles; getSoftwareName } from './functions'
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// TODO nf-core: All of these TODO statements can be deleted after the relevant changes have been made.
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// TODO nf-core: If in doubt look at other nf-core/modules to see how we are doing things! :)
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// https://github.com/nf-core/modules/tree/master/software
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// You can also ask for help via your pull request or on the #modules channel on the nf-core Slack workspace:
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// https://nf-co.re/join
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// TODO nf-core: The key words "MUST", "MUST NOT", "SHOULD", etc. are to be interpreted as described in RFC 2119 (https://tools.ietf.org/html/rfc2119).
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// TODO nf-core: A module file SHOULD only define input and output files as command-line parameters.
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// All other parameters MUST be provided as a string i.e. "options.args"
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// where "options" is a Groovy Map that MUST be provided in the "input:" section of the process.
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// Any parameters that need to be evaluated in the context of a particular sample
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// e.g. single-end/paired-end data MUST also be defined and evaluated appropriately.
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// TODO nf-core: Software that can be piped together SHOULD be added to separate module files
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// unless there is a run-time, storage advantage in implementing in this way
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// e.g. bwa mem | samtools view -B -T ref.fasta to output BAM instead of SAM.
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// TODO nf-core: Optional inputs are not currently supported by Nextflow. However, "fake files" MAY be used to work around this issue.
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// TODO nf-core: Process name MUST be all uppercase,
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// "SOFTWARE" and (ideally) "TOOL" MUST be all one word separated by an "_".
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process SOFTWARE_TOOL {
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// TODO nf-core: If a meta map of sample information is NOT provided in "input:" section
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// change tag value to another appropriate input value e.g. tag "$fasta"
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tag "$meta.id"
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// TODO nf-core: Provide appropriate resource label for process as listed in the nf-core pipeline template below:
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// https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/%7B%7Bcookiecutter.name_noslash%7D%7D/conf/base.config#L29
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label 'process_medium'
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publishDir "${params.outdir}",
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mode: params.publish_dir_mode,
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// TODO nf-core: If a meta map of sample information is NOT provided in "input:" section
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// change "publish_id:meta.id" to initialise an empty string e.g. "publish_id:''".
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saveAs: { filename -> saveFiles(filename:filename, options:options, publish_dir:getSoftwareName(task.process), publish_id:meta.id) }
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// TODO nf-core: Fetch "docker pull" address for latest BioContainer image of software: e.g. https://biocontainers.pro/#/tools/samtools
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// Click on the Pacakages and Containers tab, sort by Version and get the portion of the link after the docker pull command where Type is Docker.
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// You may need to double-check that you are using the latest version of the software because you may find that containers for older versions have been rebuilt more recently.
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// If required, multi-tool containers may also be available and are usually named to start with "mulled".
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container "quay.io/biocontainers/samtools:1.10--h9402c20_2"
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// TODO nf-core: List required Conda packages.
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// Software MUST be pinned to channel (i.e. "bioconda") and version (i.e. "1.10") as in the example below.
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// Pinning the build too e.g. "bioconda::samtools=1.10=h9402c20_2" is not currently a requirement.
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conda (params.conda ? "bioconda::samtools=1.10" : null)
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input:
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// TODO nf-core: Where applicable all sample-specific information e.g. "id", "single_end", "read_group"
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// MUST be provided as an input via a Groovy Map called "meta".
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// This information may not be required in some instances e.g. indexing reference genome files:
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// https://github.com/nf-core/modules/blob/master/software/bwa/index/main.nf
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// TODO nf-core: Where applicable please provide/convert compressed files as input/output
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// e.g. "*.fastq.gz" and NOT "*.fastq", "*.bam" and NOT "*.sam" etc.
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tuple val(meta), path(reads)
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// TODO nf-core: List additional required input channels/values here
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val options
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output:
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// TODO nf-core: Named file extensions MUST be emitted for ALL output channels
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// TODO nf-core: If meta is provided in "input:" section then it MUST be added to ALL output channels (except version)
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tuple val(meta), path("*.bam"), emit: bam
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// TODO nf-core: List additional required output channels/values here
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path "*.version.txt" , emit: version
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script:
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def software = getSoftwareName(task.process)
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def ioptions = initOptions(options)
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// TODO nf-core: If a meta map of sample information is NOT provided in "input:" section delete the line below
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def prefix = ioptions.suffix ? "${meta.id}${ioptions.suffix}" : "${meta.id}"
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// TODO nf-core: Where possible, a command MUST be provided to obtain the version number of the software e.g. 1.10
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// If the software is unable to output a version number on the command-line then it can be manually specified
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// e.g. https://github.com/nf-core/modules/blob/master/software/homer/annotatepeaks/main.nf
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// TODO nf-core: It MUST be possible to pass additional parameters to the tool as a command-line string via the "$ioptions.args" variable
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// TODO nf-core: If the tool supports multi-threading then you MUST provide the appropriate parameter
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// using the Nextflow "task" variable e.g. "--threads $task.cpus"
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// TODO nf-core: Please indent the command appropriately (4 spaces!!) to help with readability ;)
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"""
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software tool \\
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$ioptions.args \\
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--threads $task.cpus \\
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$reads \\
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> ${prefix}.bam
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echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' > ${software}.version.txt
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"""
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}
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