mirror of
https://github.com/MillironX/nf-core_modules.git
synced 2024-12-23 03:28:17 +00:00
79a9d5e1ea
NGSCheckMate ncm mode, working on bam files and vcf files to check that (human) samples match as expected Co-authored-by: Simon Pearce <simon.pearce@cruk.manchester.ac.uk> Co-authored-by: Mahesh Binzer-Panchal <mahesh.binzer-panchal@nbis.se>
49 lines
1.4 KiB
Text
49 lines
1.4 KiB
Text
process NGSCHECKMATE_NCM {
|
|
label 'process_low'
|
|
|
|
conda (params.enable_conda ? "bioconda::ngscheckmate=1.0.0" : null)
|
|
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
|
'https://depot.galaxyproject.org/singularity/ngscheckmate:1.0.0--py27r41hdfd78af_3':
|
|
'quay.io/biocontainers/ngscheckmate:1.0.0--py27r41hdfd78af_3' }"
|
|
|
|
input:
|
|
path files
|
|
path snp_bed
|
|
path fasta
|
|
|
|
output:
|
|
path "*.pdf" , emit: pdf
|
|
path "*_corr_matrix.txt", emit: corr_matrix
|
|
path "*_matched.txt" , emit: matched
|
|
path "*_all.txt" , emit: all
|
|
path "*.vcf" , emit: vcfs, optional: true
|
|
path "versions.yml" , emit: versions
|
|
|
|
when:
|
|
task.ext.when == null || task.ext.when
|
|
|
|
script:
|
|
def args = task.ext.args ?: ''
|
|
def prefix = task.ext.prefix ?: "output"
|
|
def unzip = files.any { it.toString().endsWith(".vcf.gz") }
|
|
"""
|
|
if $unzip
|
|
then
|
|
for VCFGZ in *.vcf.gz; do
|
|
gunzip -cdf \$VCFGZ > \$( basename \$VCFGZ .gz );
|
|
done
|
|
fi
|
|
|
|
NCM_REF="./"${fasta} ncm.py -d . -bed ${snp_bed} -O . -N ${prefix} $args
|
|
|
|
if $unzip
|
|
then
|
|
rm -f *.vcf # clean up decompressed vcfs
|
|
fi
|
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
"${task.process}":
|
|
ngscheckmate: \$(ncm.py --help | sed "7!d;s/ *Ensuring Sample Identity v//g")
|
|
END_VERSIONS
|
|
"""
|
|
}
|