mirror of
https://github.com/MillironX/nf-core_modules.git
synced 2024-12-23 03:28:17 +00:00
53 lines
1.7 KiB
Text
53 lines
1.7 KiB
Text
process PICARD_ADDORREPLACEREADGROUPS {
|
|
tag "$meta.id"
|
|
label 'process_low'
|
|
|
|
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
|
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
|
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
|
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
|
|
|
input:
|
|
tuple val(meta), path(bam)
|
|
|
|
output:
|
|
tuple val(meta), path("*.bam"), emit: bam
|
|
path "versions.yml" , emit: versions
|
|
|
|
when:
|
|
task.ext.when == null || task.ext.when
|
|
|
|
script:
|
|
def args = task.ext.args ?: ''
|
|
def prefix = task.ext.prefix ?: "${meta.id}"
|
|
def ID = task.ext.id ?: "id"
|
|
def LIBRARY= task.ext.library ?: "library"
|
|
def PLATFORM= task.ext.platform ?: "illumina"
|
|
def BARCODE= task.ext.barcode ?: "barcode"
|
|
def SAMPLE= task.ext.sample ?: "sample"
|
|
def INDEX= task.ext.index ?: "index"
|
|
def avail_mem = 3
|
|
if (!task.memory) {
|
|
log.info '[Picard AddOrReplaceReadGroups] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
|
|
} else {
|
|
avail_mem = task.memory.giga
|
|
}
|
|
"""
|
|
picard \\
|
|
AddOrReplaceReadGroups \\
|
|
-Xmx${avail_mem}g \\
|
|
--INPUT ${bam} \\
|
|
--OUTPUT ${prefix}.bam \\
|
|
--RGID ${ID} \\
|
|
--RGLB ${LIBRARY} \\
|
|
--RGPL ${PLATFORM} \\
|
|
--RGPU ${BARCODE} \\
|
|
--RGSM ${SAMPLE} \\
|
|
--CREATE_INDEX true
|
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
"${task.process}":
|
|
picard: \$(picard AddOrReplaceReadGroups --version 2>&1 | grep -o 'Version:.*' | cut -f2- -d:)
|
|
END_VERSIONS
|
|
"""
|
|
}
|