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63 lines
2.2 KiB
Text
63 lines
2.2 KiB
Text
process HIFIASM {
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tag "$meta.id"
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label 'process_high'
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conda (params.enable_conda ? "bioconda::hifiasm=0.15.4" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/hifiasm:0.15.4--h2e03b76_0' :
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'quay.io/biocontainers/hifiasm:0.15.4--h2e03b76_0' }"
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input:
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tuple val(meta), path(reads)
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path paternal_kmer_dump
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path maternal_kmer_dump
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val use_parental_kmers
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output:
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tuple val(meta), path("*.r_utg.gfa") , emit: raw_unitigs
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tuple val(meta), path("*.ec.bin") , emit: corrected_reads
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tuple val(meta), path("*.ovlp.source.bin") , emit: source_overlaps
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tuple val(meta), path("*.ovlp.reverse.bin"), emit: reverse_overlaps
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tuple val(meta), path("*.p_utg.gfa") , emit: processed_unitigs, optional: true
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tuple val(meta), path("*.asm.p_ctg.gfa") , emit: primary_contigs , optional: true
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tuple val(meta), path("*.asm.a_ctg.gfa") , emit: alternate_contigs, optional: true
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tuple val(meta), path("*.hap1.p_ctg.gfa") , emit: paternal_contigs , optional: true
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tuple val(meta), path("*.hap2.p_ctg.gfa") , emit: maternal_contigs , optional: true
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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if (use_parental_kmers) {
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"""
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hifiasm \\
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$args \\
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-o ${prefix}.asm \\
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-t $task.cpus \\
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-1 $paternal_kmer_dump \\
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-2 $maternal_kmer_dump \\
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$reads
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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hifiasm: \$(hifiasm --version 2>&1)
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END_VERSIONS
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"""
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} else { // Phasing with Hi-C data is not supported yet
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"""
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hifiasm \\
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$args \\
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-o ${prefix}.asm \\
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-t $task.cpus \\
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$reads
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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hifiasm: \$(hifiasm --version 2>&1)
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END_VERSIONS
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"""
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}
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}
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