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79a9d5e1ea
NGSCheckMate ncm mode, working on bam files and vcf files to check that (human) samples match as expected Co-authored-by: Simon Pearce <simon.pearce@cruk.manchester.ac.uk> Co-authored-by: Mahesh Binzer-Panchal <mahesh.binzer-panchal@nbis.se>
49 lines
1.4 KiB
Text
49 lines
1.4 KiB
Text
process NGSCHECKMATE_NCM {
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label 'process_low'
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conda (params.enable_conda ? "bioconda::ngscheckmate=1.0.0" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/ngscheckmate:1.0.0--py27r41hdfd78af_3':
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'quay.io/biocontainers/ngscheckmate:1.0.0--py27r41hdfd78af_3' }"
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input:
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path files
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path snp_bed
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path fasta
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output:
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path "*.pdf" , emit: pdf
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path "*_corr_matrix.txt", emit: corr_matrix
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path "*_matched.txt" , emit: matched
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path "*_all.txt" , emit: all
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path "*.vcf" , emit: vcfs, optional: true
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "output"
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def unzip = files.any { it.toString().endsWith(".vcf.gz") }
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"""
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if $unzip
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then
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for VCFGZ in *.vcf.gz; do
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gunzip -cdf \$VCFGZ > \$( basename \$VCFGZ .gz );
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done
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fi
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NCM_REF="./"${fasta} ncm.py -d . -bed ${snp_bed} -O . -N ${prefix} $args
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if $unzip
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then
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rm -f *.vcf # clean up decompressed vcfs
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fi
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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ngscheckmate: \$(ncm.py --help | sed "7!d;s/ *Ensuring Sample Identity v//g")
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END_VERSIONS
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"""
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}
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