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c1eb9cce44
* Trimmomatic main only first draft * Add test files * SE PE Adjustment * Remove extra reads input * chore: Remove TODOs * Apply suggestions from code review Co-authored-by: Edmund Miller <edmund.a.miller@gmail.com> * fix(trimmomatic): Handle SE output correctly Since there's never going to be unpaired reads for SE reads we can get away with it for SE * fix(trimmomatic): Use correct elvis operator to handle logic * fix(trimmomatic): Add hack to work with SE and PE reads * Update test.yml * use the PE and SE trimming correctly * Made user set adaptors * Add documentation * test(trimmomatic): Add files to pytest_modules * test(trimmomatic): Update name of failing test Co-authored-by: Edmund Miller <edmund.a.miller@protonmail.com> Co-authored-by: Edmund Miller <edmund.a.miller@gmail.com>
46 lines
1.6 KiB
Text
46 lines
1.6 KiB
Text
process TRIMMOMATIC {
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tag "$meta.id"
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label 'process_medium'
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conda (params.enable_conda ? "bioconda::trimmomatic=0.39" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/trimmomatic:0.39--hdfd78af_2':
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'quay.io/biocontainers/trimmomatic:0.39--hdfd78af_2' }"
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input:
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tuple val(meta), path(reads)
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output:
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tuple val(meta), path("*.paired.trim*.fastq.gz") , emit: trimmed_reads
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tuple val(meta), path("*.unpaired.trim_*.fastq.gz"), optional:true, emit: unpaired_reads
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tuple val(meta), path("*.log") , emit: log
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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def trimmed = meta.single_end ? "SE" : "PE"
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def output = meta.single_end ?
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"${prefix}.SE.paired.trim.fastq.gz" // HACK to avoid unpaired and paired in the trimmed_reads output
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: "${prefix}.paired.trim_1.fastq.gz ${prefix}.unpaired.trim_1.fastq.gz ${prefix}.paired.trim_2.fastq.gz ${prefix}.unpaired.trim_2.fastq.gz"
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// TODO Give better error output
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def qual_trim = task.ext.args2 ?: ''
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"""
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trimmomatic \\
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$trimmed \\
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-threads $task.cpus \\
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-trimlog ${prefix}.log \\
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$reads \\
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$output \\
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$qual_trim \\
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$args
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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trimmomatic: \$(trimmomatic -version)
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END_VERSIONS
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"""
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}
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