nf-core_modules/tests/modules/fastp/main.nf
Adam Talbot 7e8ad56688
module FASTP: Support for interleaved FASTQ (#1891)
* 1882 FASTP now supports interleaved FASTQ files

Changes:
 - single_end FASTP pipes the FASTQ file
 - Using args, it can be configured for interleaved in `--interleaved_in`
 - Out is automatically interleaved if input is paired end.
 - Removed md5sum checks for FASTQ files as compression seemed to cause
 differences
 - Instead, we check inside the FASTQ files for content.

Relates to #1882

Co-authored-by: Matthias De Smet <11850640+matthdsm@users.noreply.github.com>
2022-07-19 16:27:15 +01:00

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Text

#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
include { FASTP } from '../../../modules/fastp/main.nf'
//
// Test with single-end data
//
workflow test_fastp_single_end {
input = [ [ id:'test', single_end:true ], // meta map
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
]
save_trimmed_fail = false
save_merged = false
FASTP ( input, save_trimmed_fail, save_merged )
}
//
// Test with paired-end data
//
workflow test_fastp_paired_end {
input = [ [ id:'test', single_end:false ], // meta map
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
]
save_trimmed_fail = false
save_merged = false
FASTP ( input, save_trimmed_fail, save_merged )
}
//
// Test with intereleaved data
//
workflow test_fastp_interleaved {
input = [ [ id:'test', single_end:true ], // meta map
[ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ]
]
save_trimmed_fail = false
save_merged = false
FASTP ( input, save_trimmed_fail, save_merged )
}
//
// Test with single-end data with saving trimming fails
//
workflow test_fastp_single_end_trim_fail {
input = [ [ id:'test', single_end:true ], // meta map
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ]
]
save_trimmed_fail = true
save_merged = false
FASTP ( input, save_trimmed_fail, save_merged )
}
//
// Test with paired-end data with saving trimming fails
//
workflow test_fastp_paired_end_trim_fail {
input = [ [ id:'test', single_end:false ], // meta map
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
]
save_trimmed_fail = true
save_merged = false
FASTP ( input, save_trimmed_fail, save_merged )
}
//
// Test with paired-end data with merging
//
workflow test_fastp_paired_end_merged {
input = [ [ id:'test', single_end:false ], // meta map
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true),
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
]
save_trimmed_fail = false
save_merged = true
FASTP ( input, save_trimmed_fail, save_merged )
}