d6244b42f5
* First commit * putting correct links for singularity and docker containers (just had to search for bioconda+ascat to find them, and then put them in like the rest of the nf-core tools had it * adding first try of relevant commands (not working yet, just took their basic pipeline example * test commit * remove test * starting up work with module after 3.0.0 upgrade * add ascat.prepareHTS statemet * add location of docker for new mulled alleleCounter+ASCAT container * first full run with ASCAT on HG00154.mapped.ILLUMINA.bwa.GBR.low_coverage.20101123.bam * add notes on dropbox download * use a newer pytest_modules.yml * add outpit * trying to align with current Sarek output * adding in FH comments * busy clearing up arguments and testing. Still WIP * first working run, in nextflow, with sarek-like output. Still needs more work on input arguments * cleaning up before writing up findings * testing with putting in arguments in args * draft for solution 3 style for arguments * one more test added * adding FH map * finished testing maps for args * wrap-up cram/crai test successfully * updates to address ability to put in ref.fasta argument for cram running * adding remaining import-HTS commands in as args, and removing the chr21/chr22 only testing to test-nextflow.config * first test with auto-downloading the s3-data (when not given as an argument) * removing download-logic for supporting files, documenting in meta.yml, fixing ref_fasta bug * adding mulled singularity container * removing tests * fix left padding lint issue * lint failure in meta.yml * more linting errors * add when argument * adding stub functionality * add stub run * correct md5sum for versions.yml * more testing with -runstub * stub code in pure bash - not mixed with R * reformat version.yml * get rid of absolute paths in test.yml * correct wrong md5sum * adding allelecount conda link * rename normal_bam to input_bam etc * let the pipeline dev worry about matching the right loci and allele files * dont hardcode default genomebuild * adding download instruction comment * add doi * fix conda addition bug * add args documentation * test new indent * new test with meta.yml indentation * retry with new meta.yml * retry with new meta.yml - now with empty lines around * retry with new meta.yml - remove trailing whitepsace * trying to fix found quote character that cannot start any token error * try with one empty line above triple-quote and no empty line below * trying with pipe character * checking if its the ending triple quote * one more try with meta.yml * test update bioconda versioning for linting failure * test update bioconda versioning for linting failure 2 * testing allelecounter version error on conda Co-authored-by: @lassefolkersen Co-authored-by: @FriederikeHanssen |
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| .editorconfig | ||
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| .gitignore | ||
| .gitpod.yml | ||
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| LICENSE | ||
| main.nf | ||
| README.md | ||
THIS REPOSITORY IS UNDER ACTIVE DEVELOPMENT. SYNTAX, ORGANISATION AND LAYOUT MAY CHANGE WITHOUT NOTICE!
A repository for hosting Nextflow DSL2 module files containing tool-specific process definitions and their associated documentation.
Table of contents
Using existing modules
The module files hosted in this repository define a set of processes for software tools such as fastqc, bwa, samtools etc. This allows you to share and add common functionality across multiple pipelines in a modular fashion.
We have written a helper command in the nf-core/tools package that uses the GitHub API to obtain the relevant information for the module files present in the modules/ directory of this repository. This includes using git commit hashes to track changes for reproducibility purposes, and to download and install all of the relevant module files.
-
Install the latest version of
nf-core/tools(>=2.0) -
List the available modules:
$ nf-core modules list remote ,--./,-. ___ __ __ __ ___ /,-._.--~\ |\ | |__ __ / ` / \ |__) |__ } { | \| | \__, \__/ | \ |___ \`-._,-`-, `._,._,' nf-core/tools version 2.0 INFO Modules available from nf-core/modules (master): pipeline_modules.py:164 ┏━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┓ ┃ Module Name ┃ ┡━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━┩ │ bandage/image │ │ bcftools/consensus │ │ bcftools/filter │ │ bcftools/isec │ ..truncated.. -
Install the module in your pipeline directory:
$ nf-core modules install fastqc ,--./,-. ___ __ __ __ ___ /,-._.--~\ |\ | |__ __ / ` / \ |__) |__ } { | \| | \__, \__/ | \ |___ \`-._,-`-, `._,._,' nf-core/tools version 2.0 INFO Installing fastqc pipeline_modules.py:213 INFO Downloaded 3 files to ./modules/nf-core/modules/fastqc pipeline_modules.py:236 -
Import the module in your Nextflow script:
#!/usr/bin/env nextflow nextflow.enable.dsl = 2 include { FASTQC } from './modules/nf-core/modules/fastqc/main' -
Remove the module from the pipeline repository if required:
$ nf-core modules remove fastqc ,--./,-. ___ __ __ __ ___ /,-._.--~\ |\ | |__ __ / ` / \ |__) |__ } { | \| | \__, \__/ | \ |___ \`-._,-`-, `._,._,' nf-core/tools version 2.0 INFO Removing fastqc pipeline_modules.py:271 INFO Successfully removed fastqc pipeline_modules.py:285 -
Check that a locally installed nf-core module is up-to-date compared to the one hosted in this repo:
$ nf-core modules lint fastqc ,--./,-. ___ __ __ __ ___ /,-._.--~\ |\ | |__ __ / ` / \ |__) |__ } { | \| | \__, \__/ | \ |___ \`-._,-`-, `._,._,' nf-core/tools version 2.0 INFO Linting pipeline: . lint.py:104 INFO Linting module: fastqc lint.py:106 ╭─────────────────────────────────────────────────────────────────────────────────╮ │ [!] 1 Test Warning │ ╰─────────────────────────────────────────────────────────────────────────────────╯ ╭──────────────┬───────────────────────────────┬──────────────────────────────────╮ │ Module name │ Test message │ File path │ ├──────────────┼───────────────────────────────┼──────────────────────────────────┤ │ fastqc │ Local copy of module outdated │ modules/nf-core/modules/fastqc/ │ ╰──────────────┴────────────────────────────── ┴──────────────────────────────────╯ ╭──────────────────────╮ │ LINT RESULTS SUMMARY │ ├──────────────────────┤ │ [✔] 15 Tests Passed │ │ [!] 1 Test Warning │ │ [✗] 0 Test Failed │ ╰──────────────────────╯
Adding new modules
If you wish to contribute a new module, please see the documentation on the nf-core website.
Please be kind to our code reviewers and submit one pull request per module :)
Help
For further information or help, don't hesitate to get in touch on Slack #modules channel (you can join with this invite).
Citation
If you use the module files in this repository for your analysis please you can cite the nf-core publication as follows:
The nf-core framework for community-curated bioinformatics pipelines.
Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.
Nat Biotechnol. 2020 Feb 13. doi: 10.1038/s41587-020-0439-x.