nf-core_modules/modules/strelka/germline/main.nf
2022-02-04 09:53:32 +01:00

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process STRELKA_GERMLINE {
tag "$meta.id"
label 'process_high'
conda (params.enable_conda ? "bioconda::strelka=2.9.10" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/strelka:2.9.10--0' :
'quay.io/biocontainers/strelka:2.9.10--0' }"
input:
tuple val(meta), path(input), path(input_index)
path fasta
path fai
path target_bed
path target_bed_tbi
output:
tuple val(meta), path("*variants.vcf.gz") , emit: vcf
tuple val(meta), path("*variants.vcf.gz.tbi"), emit: vcf_tbi
tuple val(meta), path("*genome.vcf.gz") , emit: genome_vcf
tuple val(meta), path("*genome.vcf.gz.tbi") , emit: genome_vcf_tbi
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def prefix = task.ext.prefix ?: "${meta.id}"
def regions = target_bed ? "--exome --callRegions ${target_bed}" : ""
"""
configureStrelkaGermlineWorkflow.py \\
--bam $input \\
--referenceFasta $fasta \\
$regions \\
$args \\
--runDir strelka
python strelka/runWorkflow.py -m local -j $task.cpus
mv strelka/results/variants/genome.*.vcf.gz ${prefix}.genome.vcf.gz
mv strelka/results/variants/genome.*.vcf.gz.tbi ${prefix}.genome.vcf.gz.tbi
mv strelka/results/variants/variants.vcf.gz ${prefix}.variants.vcf.gz
mv strelka/results/variants/variants.vcf.gz.tbi ${prefix}.variants.vcf.gz.tbi
cat <<-END_VERSIONS > versions.yml
"${task.process}":
strelka: \$( configureStrelkaGermlineWorkflow.py --version )
END_VERSIONS
"""
}