A repository for hosting nextflow DSL2 module files containing tool-specific process definitions and associated documentation.

DSL2 IS AN EXPERIMENTAL FEATURE UNDER DEVELOPMENT. SYNTAX, ORGANISATION AND LAYOUT OF THIS REPOSITORY MAY CHANGE IN THE NEAR FUTURE!

Table of contents

• Using an existing module
  • Configuration and parameters
  • Offline usage
• Adding a new module
  • Testing
  • Documentation
  • Uploading to nf-core/modules
• Help

Using an existing module

The Nextflow -c parameter can be used with nf-core pipelines in order to load custom config files that you have available locally. However, if you or other people within your organisation are likely to be running nf-core pipelines regularly it may be a good idea to use/create a custom config file that defines some generic settings unique to the computing environment within your organisation.

Configuration and parameters

The config files hosted in this repository define a set of parameters which are specific to compute environments at different Institutions but generic enough to be used with all nf-core pipelines.

All nf-core pipelines inherit the functionality provided by Nextflow, and as such custom config files can contain parameters/definitions that are available to both. For example, if you have the ability to use Singularity on your HPC you can add and customise the Nextflow singularity scope in your config file. Similarly, you can define a Nextflow executor depending on the job submission process available on your cluster. In contrast, the params section in your custom config file will typically define parameters that are specific to nf-core pipelines.

You should be able to get a good idea as to how other people are customising the execution of their nf-core pipelines by looking at some of the config files in nf-core/modules.

Offline usage

If you want to use an existing config available in nf-core/modules, and you're running on a system that has no internet connection, you'll need to download the config file and place it in a location that is visible to the file system on which you are running the pipeline. Then run the pipeline with --custom_config_base or params.custom_config_base set to the location of the directory containing the repository files:

## Download and unzip the config files
cd /path/to/my/modules
wget https://github.com/nf-core/modules/archive/master.zip
unzip master.zip

## Run the pipeline
cd /path/to/my/data
nextflow run /path/to/pipeline/ --custom_config_base /path/to/my/modules/modules-master/

Alternatively, instead of using the configuration profiles from this repository, you can run your pipeline directly calling the single institutional config file that you need with the -c parameter.

nextflow run /path/to/pipeline/ -c /path/to/my/modules/modules-master/conf/my_config.config

Note that the nf-core/tools helper package has a download command to download all required pipeline files + singularity containers + institutional modules in one go for you, to make this process easier.

Adding a new config

If you decide to upload your module file to nf-core/module then this will ensure that it will be automatically downloaded, and available at run-time to all nf-core pipelines, and to everyone within the community. You will simply have to specify -profile <config_name> in the command used to run the pipeline. See nf-core/modules for examples.

Please also make sure to add an extra params section with params.config_profile_description, params.config_profile_contact and params.config_profile_url set to reasonable values. Users will get information on who wrote the configuration profile then when executing a nf-core pipeline and can report back if there are things missing for example.

Testing

If you want to add a new custom config file to nf-core/modules please test that your pipeline of choice runs as expected by using the -c parameter.

## Example command for nf-core/rnaseq
nextflow run nf-core/rnaseq --reads '*_R{1,2}.fastq.gz' --genome GRCh37 -c '[path to custom config]'

Documentation

You will have to create a Markdown document outlining the details required to use the custom config file within your organisation. You might orientate yourself using the Template that we provide and filling out the information for your cluster there.

See nf-core/modules/docs for examples.

Uploading to nf-core/modules

Fork the nf-core/modules repository to your own GitHub account. Within the local clone of your fork add the custom config file to the modules/ directory, and the documentation file to the docs/ directory. Please keep the naming consistent between the module and documentation files e.g. bwa.nf and bwa.md, respectively.

Commit and push these changes to your local clone on GitHub, and then create a pull request on the nf-core/modules GitHub repo with the appropriate information.

We will be notified automatically when you have created your pull request, and providing that everything adheres to nf-core guidelines we will endeavour to approve your pull request as soon as possible.

Help

If you have any questions or issues please send us a message on Slack.