nf-core_modules/modules/bismark/methylationextractor/main.nf
2022-02-04 09:53:32 +01:00

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process BISMARK_METHYLATIONEXTRACTOR {
tag "$meta.id"
label 'process_high'
conda (params.enable_conda ? "bioconda::bismark=0.23.0" : null)
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
'https://depot.galaxyproject.org/singularity/bismark:0.23.0--0' :
'quay.io/biocontainers/bismark:0.23.0--0' }"
input:
tuple val(meta), path(bam)
path index
output:
tuple val(meta), path("*.bedGraph.gz") , emit: bedgraph
tuple val(meta), path("*.txt.gz") , emit: methylation_calls
tuple val(meta), path("*.cov.gz") , emit: coverage
tuple val(meta), path("*_splitting_report.txt"), emit: report
tuple val(meta), path("*.M-bias.txt") , emit: mbias
path "versions.yml" , emit: versions
when:
task.ext.when == null || task.ext.when
script:
def args = task.ext.args ?: ''
def seqtype = meta.single_end ? '-s' : '-p'
"""
bismark_methylation_extractor \\
--bedGraph \\
--counts \\
--gzip \\
--report \\
$seqtype \\
$args \\
$bam
cat <<-END_VERSIONS > versions.yml
"${task.process}":
bismark: \$(echo \$(bismark -v 2>&1) | sed 's/^.*Bismark Version: v//; s/Copyright.*\$//')
END_VERSIONS
"""
}