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43 lines
1.4 KiB
Text
43 lines
1.4 KiB
Text
process BISMARK_METHYLATIONEXTRACTOR {
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tag "$meta.id"
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label 'process_high'
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conda (params.enable_conda ? "bioconda::bismark=0.23.0" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/bismark:0.23.0--0' :
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'quay.io/biocontainers/bismark:0.23.0--0' }"
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input:
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tuple val(meta), path(bam)
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path index
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output:
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tuple val(meta), path("*.bedGraph.gz") , emit: bedgraph
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tuple val(meta), path("*.txt.gz") , emit: methylation_calls
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tuple val(meta), path("*.cov.gz") , emit: coverage
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tuple val(meta), path("*_splitting_report.txt"), emit: report
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tuple val(meta), path("*.M-bias.txt") , emit: mbias
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def seqtype = meta.single_end ? '-s' : '-p'
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"""
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bismark_methylation_extractor \\
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--bedGraph \\
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--counts \\
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--gzip \\
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--report \\
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$seqtype \\
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$args \\
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$bam
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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bismark: \$(echo \$(bismark -v 2>&1) | sed 's/^.*Bismark Version: v//; s/Copyright.*\$//')
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END_VERSIONS
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"""
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}
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