https://millironx.com/Recent content in Home on MillironXHugoen-usMon, 23 Oct 2023 00:00:00 +0000https://millironx.com/academia/taxprofiler/Mon, 23 Oct 2023 00:00:00 +0000https://millironx.com/academia/taxprofiler/Metagenomic classification tackles the problem of characterising the taxonomic source of all DNA sequencing reads in a sample. A common approach to address the differences and biases between the many different taxonomic classification tools is to run metagenomic data through multiple classification tools and databases. This, however, is a very time-consuming task when performed manually - particularly when combined with the appropriate preprocessing of sequencing reads before the classification. Here we present nf-core/taxprofiler, a highly parallelised read-processing and taxonomic classification pipeline.https://millironx.com/academia/hydronium-pva/Fri, 02 Sep 2022 00:00:00 +0000https://millironx.com/academia/hydronium-pva/Bioremediation of chlorinated aliphatic hydrocarbon-contaminated aquifers can be hindered by high contaminant concentrations and acids generated during remediation. Encapsulating microbes in hydrogels may provide a protective, tunable environment from inhibiting compounds; however, current approaches to formulate successful encapsulated systems rely on trial and error rather than engineering approaches because fundamental information on mass-transfer coefficients is lacking. To address this knowledge gap, hydronium ion mass-transfer rates through two commonly used hydrogel materials, poly(vinyl alcohol) and alginic acid, under two solidification methods (chemical and cryogenic) were measured.https://millironx.com/academia/rotavirus-virome/Wed, 27 Apr 2022 00:00:00 +0000https://millironx.com/academia/rotavirus-virome/Enteric disease is the predominant cause of morbidity and mortality in young mammals including pigs. Viral species involved in porcine enteric disease complex (PEDC) include rotaviruses, coronaviruses, picornaviruses, astroviruses and pestiviruses among others. The virome of three groups of swine samples submitted to the Kansas State University Veterinary Diagnostic Laboratory for routine testing were assessed, namely, a Rotavirus A positive (RVA) group, a Rotavirus co-infection (RV) group and a Rotavirus Negative (RV Neg) group.https://millironx.com/websites/millironx/Tue, 01 Mar 2022 00:00:00 +0000https://millironx.com/websites/millironx/Lo and behold: I made my own website!https://millironx.com/academia/thesis/Fri, 07 Aug 2020 00:00:00 +0000https://millironx.com/academia/thesis/Polyoxometalate clusters embedded into hydrogel biobeads may be able to solve the challenges posed by free proton generation during remediation of trichloroethylene by acting as buffers and reducing protons to hydrogen gas. In this thesis, the challenges posed by systems that contain both diffusion and reaction processes for protons are considered mathematically, and a computer simulation to was developed to prove the relationship between diaphragm cell lag period and reactive capabilities of membranes.https://millironx.com/websites/record-books/Sat, 10 Aug 2019 00:00:00 +0000https://millironx.com/websites/record-books/I constructed this site to hold the record book helps of the rebellious Albany County, Wyoming. Due to the bureaucratic lag that it takes to update, it got updated just in time for Wyoming 4-H to ditch paper record books entirely. Oh, well.https://millironx.com/academia/metagenomics/Wed, 12 Jun 2019 00:00:00 +0000https://millironx.com/academia/metagenomics/Early colonization of the rumen microbiome is critical to host health and long term performance. Factors that influence early colonization include maternal factors such as gestational nutrition and mode of delivery. Therefore, we hypothesized that late gestational nutrition and mode of delivery would influence the calf rumen microbiome. Our objectives were to determine if nutrient restriction during late gestation alters the calf rumen microbiome and determine if ruminal microbiome composition differs in calves born vaginally versus caesarean.https://millironx.com/academia/cheme-car/Tue, 14 May 2019 00:00:00 +0000https://millironx.com/academia/cheme-car/The ChemE Car That Cud showcases Wyoming’s dominant industries of agriculture and mining by utilizing rumen fluid from a cannulated beef cow to generate hydrogen to be used in a hydrogen fuel cell and radioactive cesium, a byproduct of uranium that is often obtained from Wyoming’s mines, to time the car’s stop. The concentration of cesium-137 source is measured using the radioactive decay of cesium shielded by aluminum. The painted aluminum chassis was obtained from a previous team at UW, and modified using plastic k’nex toys to adapt to the current power source and stopping mechanism.https://millironx.com/academia/pva-aiche/Mon, 29 Oct 2018 00:00:00 +0000https://millironx.com/academia/pva-aiche/Trichloroethylene (TCE), a toxic and carcinogenic contaminant, presents unique challenges for cleanup because of its water solubility, density, and volatility. Bioremediation of TCE is a promising cleanup method; however, metabolism of TCE results in acid generation that inhibits remediating microorganisms. Calcium alginate(CA)-polyvinylalcohol (PVA) hydrogels show promise for protecting remediating microbes, however diffusion of TCE or its byproducts through these polymers is unknown. To measure the effective diffusion coefficient of TCE and byproducts through hydrogel membranes, we used a modified diaphragm cell.https://millironx.com/academia/how-to-build-a-cow-cud-fuel-cell/Wed, 01 Aug 2018 00:00:00 +0000https://millironx.com/academia/how-to-build-a-cow-cud-fuel-cell/https://millironx.com/academia/pva-inbre/Tue, 31 Jul 2018 00:00:00 +0000https://millironx.com/academia/pva-inbre/Trichloroethylene (TCE) is a toxic and carcinogenic contaminant that presents unique challenges for cleanup because of its density and volatility. Use of microorganisms may be a promising remediation method, however metabolism of TCE results in acid buildup, which consequently impedes the ability of microorganisms to perform this remediation. Polyvinylalginate (PVA) shows promise as a useful shield for microorganisms carrying out bioremediation of TCE by surrounding them in a protective biofilm-like layer, however, key information is missing which relates diffusion of TCE or its metabolic products through PVA.https://millironx.com/videos/ag-olympics-reel/Thu, 16 Feb 2017 00:00:00 +0000https://millironx.com/videos/ag-olympics-reel/The Albany County Farm Bureau Young Farmer’s and Rancher’s committee asked me to come and shoot a highlight reel of the Ag Olympics booth they ran at the Wyoming Football Wyoming Needs Agriculture Day. I gave away all rights to this one except for bragging rights.https://millironx.com/videos/if-you-come-out-to-the-fair/Thu, 13 Oct 2016 00:00:00 +0000https://millironx.com/videos/if-you-come-out-to-the-fair/My extension agent asked me to make a promotional video for our county fair. This is the only film of mine that has drawn an emotional response. I think I retired after this.https://millironx.com/videos/in-the-hayfields/Mon, 05 Oct 2015 00:00:00 +0000https://millironx.com/videos/in-the-hayfields/I always get the comment that our implements are old whenever I show this video off. I just ignore the naysayers, though, and good thing, too, because a version of this video won a GoPro Award. Still waiting to see my footage on their channel, though.https://millironx.com/videos/non-verum/Fri, 12 Jun 2015 00:00:00 +0000https://millironx.com/videos/non-verum/My first documentary mockumentary. This film won 2nd place at the 2016 National 4-H Film Festival, and I’ve been told that it’s more polished than the pieces done by the local TV stations. No one seems to think it’s funny, though, so I’m not sure how successful it was. Please do me a favor and google every term you don’t understand while watching it, and perhaps you’ll fare better.