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54 lines
1.9 KiB
Markdown
54 lines
1.9 KiB
Markdown
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# Illumina
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This script downloads all FASTQs associated with a specific Run ID from Illumina's Basespace.
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## Usage
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To be run in Cygwin
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```bash
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$ dl-fastqs.sh
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```
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The script will prompt for a Run ID. Copy the Run ID from the URL in Illumina Basespace.
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Note that Ctrl+V does not paste in either Cygwin or Windows terminals. Use right-click, instead.
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## Installation
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This script requires [Cygwin](https://cygwin.com/install.html). The install process is complicated thanks to requiring native features of both Windows and Cygwin.
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1. Download and install Cygwin
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1. Select "Just Me" when asked who to install for
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2. Find and add the latest version of the "git" and "xargs" packages
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3. All other default options will work
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2. Press `Win+X`, then `I` to open a new PowerShell window
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3. Run `wget "https://api.bintray.com/content/basespace/BaseSpaceCLI-EarlyAccess-BIN/latest/\$latest/amd64-windows/bs.exe?bt_package=latest" -O $env:LOCALAPPDATA\Microsoft\WindowsApps\bs.exe`
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4. Close PowerShell
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5. Open Cygwin Console and run
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```bash
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$ BS=$(cygpath "C:\Users\\$USER\AppData\Local\Microsoft\WindowsApps\bs.exe")
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$ $BS auth
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```
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6. Copy the given link, navigate to it in a browser, and login to Illumina Basespace
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7. In Cygwin console now run
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```bash
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$ git clone https://github.com/MillironX/wphl-bioinformatics.git
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$ cp wphl-bioinformatics/illumina/dl-fastqs.sh ~
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$ chmod +x dl-fastqs.sh
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```
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8. Right-click the Windows desktop, and click "New | Shortcut..."
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9. Type `C:\cygwin64\bin\bash.exe` and click Next
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10. Type `Download Illumina Run` or something else memorable and click "Finish"
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11. Right-click the newly created shortcut and click Properties
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12. In the Target textbox, add ` --login dl-fastqs.sh` (including the space) to the existing text
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13. Click OK
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