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Make copying script combine fastqs
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commit
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1 changed files with 29 additions and 8 deletions
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@ -60,16 +60,37 @@ echo "If this is not correct, press CTRL+C within the next 10 seconds to abort..
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sleep 10
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echo "Proceeding..."
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# Simplify the output paths
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USBPATH="$USBDRIVE/$FOLDERNAME"
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FAST5PATH="$USBPATH/fast5"
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FASTQPATH="$USBPATH/fastq"
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# Make output directories
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mkdir -p "$USBDRIVE/$FOLDERNAME/fast5"
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mkdir -p "$USBDRIVE/$FOLDERNAME/fastq"
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mkdir -p "$FAST5PATH"
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mkdir -p "$FASTQPATH"
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# Copy the files
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for FASTA in ${KEEPERS[@]}; do
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cp -n $FOLDERPATH/*/fast5_pass/barcode$FASTA/*.fast5 $USBDRIVE/$FOLDERNAME/fast5 2> /dev/null
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cp -n $FOLDERPATH/*/fastq_pass/barcode$FASTA/*.fastq $USBDRIVE/$FOLDERNAME/fastq 2> /dev/null
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cp -n $FOLDERPATH/*/fastq_pass/barcode$FASTA/*.fastq.gz $USBDRIVE/$FOLDERNAME/fastq 2> /dev/null
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done
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# Check to see if there are any files here
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if [[ -n $(find $FOLDERPATH -type f \( -name "*.fast5" -o -name "*.fastq" -o -name "*.fastq.gz" \) -path "*_pass*barcode$FASTA*") ]]; then
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# Copy FAST5s
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find $FOLDERPATH -name "*.fast5" -path "*_pass*barcode$FASTA*" -exec cp -n {} $FAST5PATH \;
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# Make sure we end on a happy note, regardless if there were actually 96 barcodes
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exit 0
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# Unzip any gzipped fastqs
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find $FOLDERPATH -name "*.fastq.gz" -path "*_pass*barcode$FASTA*" -print0 | xargs -0 -r -L1 -P0 gunzip
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# Get the name of the resulting FASTQ file
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FASTQRESULT="$FASTQPATH"/"$FOLDERNAME"_pass_barcode"$FASTA".fastq
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# Concatenate the fastqs
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cat $FOLDERPATH/*/fastq_pass/barcode$FASTA/*.fastq > $FASTQRESULT
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# Zip up the remainders
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find $FOLDERPATH -name "*.fastq" -path "*_pass*barcode$FASTA*" -print0 | xargs -0 -r -L1 -P0 gzip
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# Zip up the copied fastq
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gzip $FASTQRESULT
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else
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echo "No suitable files found for Barcode $FASTA, skipping"
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fi
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done
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