Sanitize redacted name

This commit is contained in:
Thomas A. Christensen II 2021-04-28 19:27:56 -06:00
parent 471bf4314d
commit affa7796ac
Signed by: millironx
GPG key ID: 139C07724802BC5D
6 changed files with 31 additions and 24 deletions

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@ -12,6 +12,5 @@
"limsdatapull",
"miseq",
"mkdir",
"wphl"
]
}

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@ -1,10 +1,11 @@
# wphl-bioinformatics
# SC2 Sequencing Cheats
Bioinformatics scripts for use at the Wyoming Public Health Laboratory.
File manipulation/bioinformatics-type scripts that my colleuges have found
useful in sequencing SARS-CoV2 (SC2).
These scripts have various purposes, uses, and instructions. The only unifying
thing about them is that they were all written for WPHL. Please check the
`README` file in each directory for more detailed instructions.
thing about them is that they were all written for SC2 and related sequencing.
Please check the `README` file in each directory for more detailed instructions.
## Encryption
@ -49,7 +50,7 @@ Encryption is GPG-based. If your GPG key has been added to the repository (you
know if you have access), then run
```bash
git clone https://github.com/MillironX/wphl-bioinformatics.git && cd wphl-bioinformatics
git clone https://github.com/MillironX/sc2-sequencing.git && cd sc2-sequencing
git-crypt unlock
```
@ -57,4 +58,4 @@ The files will now be decrypted and can be usable.
If you need to copy your GPG keys to a new machine to be able to decrypt the
files, follow the instructions at
https://www.phildev.net/pgp/gpg_moving_keys.html under "Copy Just Your Keys."
<https://www.phildev.net/pgp/gpg_moving_keys.html> under "Copy Just Your Keys."

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@ -22,8 +22,8 @@ This script requires [Cygwin](https://cygwin.com/install.html).
2. Open the Cygwin console and use the following commands
```bash
git clone https://github.com/MillironX/wphl-bioinformatics.git
cp wphl-bioinformatics/clearlabs/convert-fastas.sh ~
git clone https://github.com/MillironX/sc2-sequencing.git
cp sc2-sequencing/clearlabs/convert-fastas.sh ~
chmod +x convert-fastas.sh
```

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@ -49,8 +49,8 @@ Cygwin.
7. In Cygwin console now run
```bash
git clone https://github.com/MillironX/wphl-bioinformatics.git
cp wphl-bioinformatics/illumina/dl-fastqs.sh ~
git clone https://github.com/MillironX/sc2-sequencing.git
cp sc2-sequencing/illumina/dl-fastqs.sh ~
chmod +x dl-fastqs.sh
```

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@ -4,7 +4,7 @@ Pulls positive SC2 sample IDs and relevant dates from StarLIMS.
**NOTE:** These scripts are encrypted, but are generally intended for use on
Windows machines. Decrypt them on a Linux/Mac machine using the [entire repo
instructions](https://github.com/MillironX/wphl-bioinformatics/blob/master/README.md),
instructions](https://github.com/MillironX/sc2-sequencing/blob/master/README.md),
then copy over using a secure flash drive or similar method.
## Usage

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@ -1,10 +1,11 @@
# Oxford Nanopore Technologies
These scripts are for use with a GridION, to make the files and folder
structures correspond more closely to those required by downstream
These scripts are for use with a GridION or its output file, to make the files
and folder structures correspond more closely to those required by downstream
bioinformatics programs. Note that at the time of writing, the GridION runs
Ubuntu 16.04 LTS, and mounts USB drives in the `/data` folder, and these scripts
contain some "faults" to work around these idiosyncrasies.
contain some "faults" to work around these idiosyncrasies (i.e. they will not
pass in [ShellCheck]).
## Usage
@ -31,7 +32,7 @@ julia --project=PATH_TO_ONT_FOLDER PATH_TO_ONT-RENAME.jl
In practice, this looks like
```powershell
julia --project=C:\Users\MillironX\ont C:\Users\MillironX\illumina\ont-rename.jl
julia --project=C:\Users\MillironX\ont C:\Users\MillironX\ont\ont-rename.jl
```
### `ont-demux` (deprecated)
@ -45,9 +46,9 @@ same results using far fewer resources and in far less time.**
Consolidates all FAST5s into a single gzipped FASTQ using ONT's
`guppy_barcoder`. Note that this is for MinKNOW's default settings of 4000
reads/file. It is _highly_ recommended to set the "Number of Reads per File"
reads/file. ~~It is _highly_ recommended to set the "Number of Reads per File"
entry in MinKNOW to 0, and checking "Compress FASTQs" to produce nearly the same
results.
results~~ (this functionality has been taken out of recent updates to MinKNOW).
## Installation
@ -56,7 +57,7 @@ results.
#### Requirements
- git
- guppy (Get from [ONT Community](https://nanoporetech.com/community)
- guppy (Get from [ONT Community])
- GNU Parallel
See ONT for guppy installation instructions. Others may be installed by opening
@ -71,17 +72,19 @@ sudo apt-get install git parallel -y
Open a terminal (Ctrl+Alt+T), and use the following commands
```bash
git clone https://github.com/MillironX/wphl-bioinformatics
git clone https://github.com/MillironX/sc2-sequencing
mkdir ~/bin
echo "export PATH=$PATH:$HOME/bin" >> ~/.bashrc
cp wphl-bioinformatics/ont/* ~/bin
cp sc2-sequending/ont/* ~/bin
chmod +x ~/bin/*
source ~/.bashrc
```
### `ont-rename`
These scripts require [Julia](https://julialang.org).
These scripts require [Julia].
Windows install instructions (I will assume Linux users can adapt):
1. Download and install Julia
1. Select "Add Julia to PATH" as an option
@ -97,6 +100,10 @@ These scripts require [Julia](https://julialang.org).
10. Run `exit()`
11. Close PowerShell
12. Right-click the Windows desktop, and click "New | Shortcut..."
13. Type `julia --project=%USERPROFILE%\ont
%USERPROFILE%\ont\ont-rename.jl` and click Next
13. Type `julia --project=%USERPROFILE%\ont %USERPROFILE%\ont\ont-rename.jl` and
click Next
14. Type `Rename ONT FASTQs` or something else memorable and click "Finish"
[ShellCheck]: https://shellcheck.net
[ONT Community]: https://nanoporetech.com/community
[Julia]: https://julialang.org