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Add samtools to VarScan image
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1 changed files with 36 additions and 3 deletions
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@ -2,8 +2,37 @@ Bootstrap: library
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From: default/alpine:3.14.0
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%post
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# Install java and bash (for Nextflow)
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apk add --no-cache openjdk8 bash
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# Part 1: Install Samtools
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# ========================
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# Get the actual dependencies
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apk add --no-cache ncurses libbz2 xz zlib
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# Get the build dependencies
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apk add --no-cache --virtual .build-deps ncurses-dev musl-dev g++ make zlib-dev bzip2-dev xz-dev
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# Make versioning easier
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SAMTOOLS_VERSION='1.13'
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# Download the source tarball and build
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cd /tmp || exit 1
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wget https://github.com/samtools/samtools/releases/download/$SAMTOOLS_VERSION/samtools-$SAMTOOLS_VERSION.tar.bz2
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tar xjvf samtools-$SAMTOOLS_VERSION.tar.bz2
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cd samtools-$SAMTOOLS_VERSION || exit 1
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./configure
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make && make install
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cd .. || exit 1
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rm -rf samtools-$SAMTOOLS_VERSION*
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cd || exit 1
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# Cleanup build packages
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apk del --no-cache .build-deps
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# Part 2: Install VarScan
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# ========================
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# Install java
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apk add --no-cache openjdk8
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# Make versioning easier
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VARSCAN_VERSION='v2.4.4'
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@ -16,10 +45,14 @@ From: default/alpine:3.14.0
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echo "java -jar /opt/varscan.jar \"\$@\"" >> /usr/local/bin/varscan
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chmod +x /usr/local/bin/varscan
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# Part 3: Install Nextflow dependencies
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# =====================================
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apk add --no-cache bash
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%test
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cat << "EOF" > /tmp/singularity-test.sh
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#!/bin/bash
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CMDS=('bash' 'ps' 'java' 'varscan')
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CMDS=('bash' 'ps' 'java' 'varscan' 'samtools')
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for CMD in "${CMDS[@]}"; do
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if ! command -v "$CMD"; then
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echo "command $CMD not found!"
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