2022-02-18 06:55:14 +00:00
|
|
|
process FASTQC {
|
|
|
|
tag "$meta.id"
|
|
|
|
label 'process_medium'
|
|
|
|
|
|
|
|
conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null)
|
|
|
|
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
|
|
|
'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' :
|
|
|
|
'quay.io/biocontainers/fastqc:0.11.9--0' }"
|
|
|
|
|
|
|
|
input:
|
|
|
|
tuple val(meta), path(reads)
|
|
|
|
|
|
|
|
output:
|
|
|
|
tuple val(meta), path("*.html"), emit: html
|
|
|
|
tuple val(meta), path("*.zip") , emit: zip
|
|
|
|
path "versions.yml" , emit: versions
|
|
|
|
|
2022-03-23 13:57:56 +00:00
|
|
|
when:
|
|
|
|
task.ext.when == null || task.ext.when
|
|
|
|
|
2022-02-18 06:55:14 +00:00
|
|
|
script:
|
|
|
|
def args = task.ext.args ?: ''
|
|
|
|
// Add soft-links to original FastQs for consistent naming in pipeline
|
|
|
|
def prefix = task.ext.prefix ?: "${meta.id}"
|
|
|
|
if (meta.single_end) {
|
|
|
|
"""
|
|
|
|
[ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
|
|
|
|
fastqc $args --threads $task.cpus ${prefix}.fastq.gz
|
|
|
|
|
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
|
|
"${task.process}":
|
|
|
|
fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
|
|
|
|
END_VERSIONS
|
|
|
|
"""
|
|
|
|
} else {
|
|
|
|
"""
|
|
|
|
[ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
|
|
|
|
[ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
|
|
|
|
fastqc $args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz
|
|
|
|
|
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
|
|
"${task.process}":
|
|
|
|
fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
|
|
|
|
END_VERSIONS
|
|
|
|
"""
|
|
|
|
}
|
2022-10-05 03:30:53 +00:00
|
|
|
|
|
|
|
stub:
|
|
|
|
def prefix = task.ext.prefix ?: "${meta.id}"
|
|
|
|
"""
|
|
|
|
touch ${prefix}.html
|
|
|
|
touch ${prefix}.zip
|
|
|
|
|
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
|
|
"${task.process}":
|
|
|
|
fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
|
|
|
|
END_VERSIONS
|
|
|
|
"""
|
2022-02-18 06:55:14 +00:00
|
|
|
}
|