2022-02-18 06:55:14 +00:00
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/*
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2022-03-15 21:05:37 +00:00
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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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2022-02-18 06:55:14 +00:00
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Config file for defining DSL2 per module options and publishing paths
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2022-03-15 21:05:37 +00:00
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~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
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2022-02-18 06:55:14 +00:00
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Available keys to override module options:
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2022-03-15 21:05:37 +00:00
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ext.args = Additional arguments appended to command in module.
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ext.args2 = Second set of arguments appended to command in module (multi-tool modules).
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ext.args3 = Third set of arguments appended to command in module (multi-tool modules).
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ext.prefix = File name prefix for output files.
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2022-02-18 06:55:14 +00:00
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----------------------------------------------------------------------------------------
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*/
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process {
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publishDir = [
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path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
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2022-03-15 21:05:37 +00:00
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mode: params.publish_dir_mode,
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2022-02-18 06:55:14 +00:00
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saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
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]
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withName: SAMPLESHEET_CHECK {
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publishDir = [
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path: { "${params.outdir}/pipeline_info" },
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2022-03-15 21:05:37 +00:00
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mode: params.publish_dir_mode,
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2022-02-18 06:55:14 +00:00
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saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
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]
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}
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2022-03-21 19:28:09 +00:00
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withName: DATABASE_CHECK {
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publishDir = [
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path: { "${params.outdir}/pipeline_info" },
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mode: params.publish_dir_mode,
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saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
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]
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}
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withName: UNTAR {
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2022-03-21 19:30:29 +00:00
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publishDir = [
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2022-03-21 19:28:09 +00:00
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path: { "${params.outdir}/databases" },
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mode: params.publish_dir_mode,
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saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
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]
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}
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2022-02-18 06:55:14 +00:00
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withName: FASTQC {
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ext.args = '--quiet'
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2022-02-18 15:51:01 +00:00
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ext.prefix = { "${meta.id}_${meta.run_accession}_raw" }
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publishDir = [
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path: { "${params.outdir}/fastqc/raw" },
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mode: 'copy',
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pattern: '*.html'
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]
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}
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2022-03-25 13:58:06 +00:00
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withName: FASTQC_PROCESSED {
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ext.args = '--quiet'
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ext.prefix = { "${meta.id}_${meta.run_accession}_processed" }
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publishDir = [
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path: { "${params.outdir}/fastqc/processed" },
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mode: 'copy',
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pattern: '*.html'
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]
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}
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2022-04-01 07:50:08 +00:00
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withName: FASTP_SINGLE {
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ext.args = [
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// trimming options
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params.shortread_clipmerge_skipadaptertrim ? "--disable_adapter_trimming" : "",
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params.shortread_clipmerge_adapter1 ? "--adapter_sequence ${params.shortread_clipmerge_adapter1}" : "",
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// filtering options
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"--length_required ${params.shortread_clipmerge_minlength}"
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].join(' ').trim()
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2022-02-18 15:51:01 +00:00
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ext.prefix = { "${meta.id}_${meta.run_accession}" }
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publishDir = [
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path: { "${params.outdir}/fastp" },
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mode: 'copy',
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pattern: '*.fastq.gz'
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]
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}
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withName: FASTP_PAIRED {
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2022-02-18 15:51:01 +00:00
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ext.args = [
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2022-03-27 07:30:23 +00:00
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// collapsing options - option to retain singletons
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2022-03-25 13:58:06 +00:00
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params.shortread_clipmerge_excludeunmerged ? '' : "--include_unmerged",
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// trimming options
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2022-03-30 09:31:37 +00:00
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params.shortread_clipmerge_skipadaptertrim ? "--disable_adapter_trimming" : "",
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2022-03-25 14:01:25 +00:00
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params.shortread_clipmerge_adapter1 ? "--adapter_sequence ${params.shortread_clipmerge_adapter1}" : "",
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2022-04-01 07:50:08 +00:00
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params.shortread_clipmerge_adapter2 ? "--adapter_sequence_r2 ${params.shortread_clipmerge_adapter2}" : "--detect_adapter_for_pe",
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2022-03-25 13:58:06 +00:00
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// filtering options
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"--length_required ${params.shortread_clipmerge_minlength}"
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2022-02-18 15:51:01 +00:00
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].join(' ').trim()
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2022-04-01 07:50:08 +00:00
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ext.prefix = { "${meta.id}_${meta.run_accession}" }
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2022-02-18 15:51:01 +00:00
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publishDir = [
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path: { "${params.outdir}/fastp" },
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mode: 'copy',
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pattern: '*.fastq.gz'
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]
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}
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2022-04-01 07:50:08 +00:00
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withName: ADAPTERREMOVAL_SINGLE {
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ext.args = [
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// trimming options
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params.shortread_clipmerge_skipadaptertrim ? "--adapter1 '' --adapter2 ''" : "",
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params.shortread_clipmerge_adapter1 ? "--adapter1 ${params.shortread_clipmerge_adapter1}" : "",
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// filtering options
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"--minlength ${params.shortread_clipmerge_minlength}"
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].join(' ').trim()
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ext.prefix = { "${meta.id}_${meta.run_accession}" }
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publishDir = [
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path: { "${params.outdir}/adapterremoval" },
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mode: 'copy',
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pattern: '*.fastq.gz'
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]
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}
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withName: ADAPTERREMOVAL_PAIRED {
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ext.args = [
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// collapsing options
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params.shortread_clipmerge_mergepairs ? "--collapse" : "",
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// trimming options
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params.shortread_clipmerge_skipadaptertrim ? "--adapter1 '' --adapter2 ''" : "",
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params.shortread_clipmerge_adapter1 ? "--adapter1 ${params.shortread_clipmerge_adapter1}" : "",
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params.shortread_clipmerge_adapter2 ? "--adapter2 ${params.shortread_clipmerge_adapter2}" : "",
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// filtering options
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"--minlength ${params.shortread_clipmerge_minlength}"
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].join(' ').trim()
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ext.prefix = { "${meta.id}_${meta.run_accession}" }
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publishDir = [
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path: { "${params.outdir}/adapterremoval" },
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mode: 'copy',
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pattern: '*.fastq.gz'
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]
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}
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2022-03-18 13:34:10 +00:00
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withName: PORECHOP {
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ext.prefix = { "${meta.id}_${meta.run_accession}" }
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publishDir = [
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path: { "${params.outdir}/porechop" },
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mode: 'copy',
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pattern: '*.fastq.gz'
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]
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2022-02-18 15:51:01 +00:00
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}
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withName: CAT_FASTQ {
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publishDir = [
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path: { "${params.outdir}/prepared_sequences" },
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mode: 'copy',
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pattern: '*.fastq.gz'
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]
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2022-02-18 06:55:14 +00:00
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}
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2022-03-03 16:42:02 +00:00
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withName: MALT_RUN {
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2022-04-01 07:50:08 +00:00
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ext.args = { "${meta.db_params}" }
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ext.prefix = { "${meta.id}-${meta.run_accession}-${meta.db_name}" }
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2022-03-03 16:42:02 +00:00
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publishDir = [
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path: { "${params.outdir}/malt/${meta.db_name}" },
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mode: 'copy',
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pattern: '*.{rma6,tab,text,sam,log}'
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]
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}
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withName: KRAKEN2_KRAKEN2 {
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2022-04-01 07:50:08 +00:00
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ext.args = { "${meta.db_params}" }
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ext.prefix = { "${meta.id}-${meta.run_accession}-${meta.db_name}" }
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2022-03-03 16:42:02 +00:00
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publishDir = [
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path: { "${params.outdir}/kraken2/${meta.db_name}" },
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mode: 'copy',
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2022-03-21 14:58:19 +00:00
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pattern: '*.{fastq.gz,txt}'
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2022-03-03 16:42:02 +00:00
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]
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2022-02-18 06:55:14 +00:00
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}
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2022-04-02 12:44:27 +00:00
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withName: METAPHLAN3 {
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publishDir = [
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2022-04-02 12:58:07 +00:00
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path: { "${params.outdir}/metaphlan3/${meta.db_name}" },
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2022-04-02 12:44:27 +00:00
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mode: params.publish_dir_mode,
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pattern: '*.{biom,txt}'
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]
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2022-04-02 12:58:07 +00:00
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ext.prefix = { "${meta.id}-${meta.run_accession}-${meta.db_name}" }
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2022-04-02 12:44:27 +00:00
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}
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2022-02-18 06:55:14 +00:00
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withName: CUSTOM_DUMPSOFTWAREVERSIONS {
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publishDir = [
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path: { "${params.outdir}/pipeline_info" },
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2022-03-15 21:05:37 +00:00
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mode: params.publish_dir_mode,
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2022-02-18 06:55:14 +00:00
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pattern: '*_versions.yml'
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]
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}
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}
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