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Update output and usage
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@ -58,7 +58,9 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
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### fastp
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### fastp
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fastp can automatically detect adapter sequences for Illumina data.
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[fastp](https://github.com/OpenGene/fastp) is a FASTQ pre-processing tool for quality control, trimmming of adapters, quality filtering and other features.
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It is used in nf-core/taxprofiler for adapter trimming of short-reads.
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<details markdown="1">
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<details markdown="1">
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<summary>Output files</summary>
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<summary>Output files</summary>
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@ -141,6 +143,8 @@ Note that the FASTQ file(s) may _not_ always be the 'final' reads that go into t
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### Filtlong
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### Filtlong
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[Filtlong](https://github.com/rrwick/Filtlong) is a quality filtering tool for long reads.
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<details markdown="1">
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<details markdown="1">
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<summary>Output files</summary>
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<summary>Output files</summary>
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@ -172,6 +176,10 @@ Note that the FASTQ file(s) may _not_ always be the 'final' reads that go into t
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### minimap2
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### minimap2
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[minimap2](https://github.com/lh3/minimap2) is an alignment tool suited to mapping long reads to reference sequences.
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It is used with nf-core/taxprofiler to allow removal of 'host' (e.g. human) or other possible contaminant reads from the FASTQ files prior to taxonomic classification/profiling.
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<details markdown="1">
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<details markdown="1">
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<summary>Output files</summary>
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<summary>Output files</summary>
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@ -182,6 +190,8 @@ Note that the FASTQ file(s) may _not_ always be the 'final' reads that go into t
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### Samtools stats
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### Samtools stats
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[Samtools stats](http://www.htslib.org/doc/samtools-stats.html) collects statistics from an alignment file and outputs in a text format.
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<details markdown="1">
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<details markdown="1">
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<summary>Output files</summary>
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<summary>Output files</summary>
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@ -254,6 +264,8 @@ You will only receive the FASTQs and `*classifiedreads.txt` file if you supply `
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### Centrifuge
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### Centrifuge
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[Centrifuge](https://github.com/DaehwanKimLab/centrifuge) is a taxonomic sequence classifier that uses a Burrows-Wheeler transform and Ferragina-Manzina index for storing and mapping sequences.
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<details markdown="1">
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<details markdown="1">
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<summary>Output files</summary>
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<summary>Output files</summary>
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@ -279,6 +291,8 @@ You will only receive the FASTQs and `*classifiedreads.txt` file if you supply `
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### DIAMOND
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### DIAMOND
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[DIAMOND](https://github.com/bbuchfink/diamond) is a sequence aligner for translated DNA searches or protein sequences against a protein reference database such as NR. It is a replacement for the NCBI BLAST software tools.It has many key features and it is used as taxonomic classifier in nf-core/taxprofiler.
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<details markdown="1">
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<details markdown="1">
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<summary>Output files</summary>
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<summary>Output files</summary>
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@ -328,6 +342,8 @@ You will only recieve the `.sam` and `.megan` files if you supply `--malt_save_r
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The main taxonomic profiling file from MetaPhlAn3 is the `*_profile.txt` file. This provides the abundance estimates from MetaPhlAn3 however does not include raw counts by default.
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The main taxonomic profiling file from MetaPhlAn3 is the `*_profile.txt` file. This provides the abundance estimates from MetaPhlAn3 however does not include raw counts by default.
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### mOTUs
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### mOTUs
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[mOTUS](https://github.com/motu-tool/mOTUs) maps reads to a unique marker specific database and estimates the relative abundance of known and unknown species.
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<details markdown="1">
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<details markdown="1">
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<summary>Output files</summary>
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<summary>Output files</summary>
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@ -631,7 +631,6 @@ krakenuniq-build --db <DB_DIR_NAME> --kmer-len 31
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Please see the [KrakenUniq documentation](https://github.com/fbreitwieser/krakenuniq#database-building) for more information.
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Please see the [KrakenUniq documentation](https://github.com/fbreitwieser/krakenuniq#database-building) for more information.
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````
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#### MALT custom database
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#### MALT custom database
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