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2 changed files with 9 additions and 43 deletions
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@ -165,9 +165,11 @@ work # Directory containing the nextflow working files
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.nextflow_log # Log file from Nextflow
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.nextflow_log # Log file from Nextflow
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# Other nextflow hidden files, eg. history of pipeline runs and old logs.
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# Other nextflow hidden files, eg. history of pipeline runs and old logs.
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```
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```
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### Sequencing quality control
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### Sequencing quality control
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nf-core taxprofiler offers [`Falco`](https://github.com/smithlabcode/falco] as an alternative option to [`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
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nf-core taxprofiler offers [`Falco`](https://github.com/smithlabcode/falco] as an alternative option to [`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
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### Preprocessing Steps
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### Preprocessing Steps
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nf-core/taxprofiler offers four main preprocessing steps
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nf-core/taxprofiler offers four main preprocessing steps
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@ -10,11 +10,7 @@
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"type": "object",
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"type": "object",
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"fa_icon": "fas fa-terminal",
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"fa_icon": "fas fa-terminal",
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"description": "Define where the pipeline should find input data and save output data.",
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"description": "Define where the pipeline should find input data and save output data.",
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"required": [
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"required": ["input", "outdir", "databases"],
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"input",
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"outdir",
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"databases"
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],
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"properties": {
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"properties": {
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"input": {
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"input": {
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"type": "string",
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"type": "string",
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@ -84,10 +80,7 @@
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"shortread_qc_tool": {
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"shortread_qc_tool": {
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"type": "string",
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"type": "string",
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"default": "fastp",
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"default": "fastp",
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"enum": [
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"enum": ["fastp", "adapterremoval"],
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"fastp",
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"adapterremoval"
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],
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"fa_icon": "fas fa-tools",
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"fa_icon": "fas fa-tools",
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"description": "Specify which tool to use for short-read QC"
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"description": "Specify which tool to use for short-read QC"
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},
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},
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@ -140,11 +133,7 @@
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"shortread_complexityfilter_tool": {
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"shortread_complexityfilter_tool": {
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"type": "string",
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"type": "string",
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"default": "bbduk",
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"default": "bbduk",
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"enum": [
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"enum": ["bbduk", "prinseqplusplus", "fastp"],
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"bbduk",
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"prinseqplusplus",
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"fastp"
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],
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"fa_icon": "fas fa-hammer",
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"fa_icon": "fas fa-hammer",
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"description": "Specify which tool to use for complexity filtering"
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"description": "Specify which tool to use for complexity filtering"
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},
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},
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@ -178,10 +167,7 @@
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"shortread_complexityfilter_prinseqplusplus_mode": {
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"shortread_complexityfilter_prinseqplusplus_mode": {
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"type": "string",
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"type": "string",
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"default": "entropy",
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"default": "entropy",
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"enum": [
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"enum": ["entropy", "dust"],
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"entropy",
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"dust"
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],
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"fa_icon": "fas fa-check-square",
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"fa_icon": "fas fa-check-square",
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"description": "Specify the complexity filter mode for PRINSEQ++"
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"description": "Specify the complexity filter mode for PRINSEQ++"
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},
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},
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"diamond_output_format": {
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"diamond_output_format": {
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"type": "string",
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"type": "string",
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"default": "tsv",
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"default": "tsv",
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"enum": [
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"enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"],
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"blast",
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"xml",
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"txt",
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"daa",
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"sam",
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"tsv",
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"paf"
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],
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"fa_icon": "fas fa-file",
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"fa_icon": "fas fa-file",
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"description": "Specify output format from DIAMOND profiling.",
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"description": "Specify output format from DIAMOND profiling.",
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"help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`"
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"help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`"
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@ -382,14 +360,7 @@
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"kaiju_taxon_rank": {
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"kaiju_taxon_rank": {
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"type": "string",
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"type": "string",
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"default": "species",
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"default": "species",
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"enum": [
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"enum": ["phylum", "class", "order", "family", "genus", "species"],
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"phylum",
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"class",
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"order",
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"family",
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"genus",
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"species"
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],
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"fa_icon": "fas fa-tag",
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"fa_icon": "fas fa-tag",
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"description": "Specify taxonomic rank to be displayed in Kaiju taxon table",
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"description": "Specify taxonomic rank to be displayed in Kaiju taxon table",
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"help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`"
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"help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`"
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@ -584,14 +555,7 @@
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"description": "Method used to save pipeline results to output directory.",
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"description": "Method used to save pipeline results to output directory.",
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"help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
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"help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
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"fa_icon": "fas fa-copy",
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"fa_icon": "fas fa-copy",
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"enum": [
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"enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"],
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"symlink",
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"rellink",
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"link",
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"copy",
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"copyNoFollow",
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"move"
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],
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"hidden": true
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"hidden": true
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},
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},
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"email_on_fail": {
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"email_on_fail": {
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