mirror of
https://github.com/MillironX/taxprofiler.git
synced 2024-11-13 07:03:10 +00:00
Start work on groovy native datbase checks
This commit is contained in:
parent
13c4f44ca6
commit
07bd989bc6
4 changed files with 30 additions and 44 deletions
|
@ -12,14 +12,6 @@
|
|||
|
||||
process {
|
||||
|
||||
withName: DATABASE_CHECK {
|
||||
publishDir = [
|
||||
path: { "${params.outdir}/pipeline_info" },
|
||||
mode: params.publish_dir_mode,
|
||||
saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
|
||||
]
|
||||
}
|
||||
|
||||
withName: FASTQC {
|
||||
ext.args = '--quiet'
|
||||
ext.prefix = { "${meta.id}_${meta.run_accession}_raw" }
|
||||
|
|
|
@ -1,29 +0,0 @@
|
|||
process DATABASE_CHECK {
|
||||
tag "$databasesheet"
|
||||
label 'process_single'
|
||||
|
||||
conda (params.enable_conda ? "conda-forge::python=3.8.3" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/python:3.8.3' :
|
||||
'quay.io/biocontainers/python:3.8.3' }"
|
||||
|
||||
input:
|
||||
path databasesheet
|
||||
|
||||
output:
|
||||
path '*.csv' , emit: csv
|
||||
path "versions.yml", emit: versions
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
||||
script: // This script is bundled with the pipeline, in nf-core/taxprofiler/bin/
|
||||
"""
|
||||
cat $databasesheet >> database_sheet.valid.csv
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
python: \$(python --version | sed 's/Python //g')
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
|
@ -2,7 +2,6 @@
|
|||
// Check input samplesheet and get read channels
|
||||
//
|
||||
|
||||
include { DATABASE_CHECK } from '../../modules/local/database_check'
|
||||
include { UNTAR } from '../../modules/nf-core/untar/main'
|
||||
|
||||
workflow DB_CHECK {
|
||||
|
@ -10,15 +9,18 @@ workflow DB_CHECK {
|
|||
dbsheet // file: /path/to/dbsheet.csv
|
||||
|
||||
main:
|
||||
ch_versions = Channel.empty()
|
||||
|
||||
// TODO: make database sheet check
|
||||
// Checks:
|
||||
// 1) no duplicates,
|
||||
// 2) args do not have quotes, e.g. just `,,` and NOT `,"",`
|
||||
parsed_samplesheet = DATABASE_CHECK ( dbsheet )
|
||||
.csv
|
||||
|
||||
parsed_samplesheet = Channel.fromPath(dbsheet)
|
||||
.splitCsv ( header:true, sep:',' )
|
||||
.map { create_db_channels(it) }
|
||||
.map {
|
||||
validate_db_sheet(it)
|
||||
create_db_channels(it)
|
||||
}
|
||||
|
||||
ch_dbs_for_untar = parsed_samplesheet
|
||||
.branch {
|
||||
|
@ -29,12 +31,31 @@ workflow DB_CHECK {
|
|||
// TODO Filter to only run UNTAR on DBs of tools actually using?
|
||||
// TODO make optional whether to save
|
||||
UNTAR ( ch_dbs_for_untar.untar )
|
||||
ch_versions = ch_versions.mix(UNTAR.out.versions.first())
|
||||
|
||||
ch_final_dbs = ch_dbs_for_untar.skip.mix( UNTAR.out.untar )
|
||||
|
||||
emit:
|
||||
dbs = ch_final_dbs // channel: [ val(meta), [ db ] ]
|
||||
versions = DATABASE_CHECK.out.versions.mix(UNTAR.out.versions.first()) // channel: [ versions.yml ]
|
||||
versions = ch_versions // channel: [ versions.yml ]
|
||||
}
|
||||
|
||||
def validate_db_sheet(LinkedHashMap row){
|
||||
|
||||
// check minimum number of columns
|
||||
if (row.size() < 4) exit 1, "[nf-core/taxprofiler] error: Invalid database input sheet - malformed row (e.g. missing column). See documentation for more information. Error in: ${row}, "
|
||||
|
||||
// all columns there
|
||||
def expected_headers = ['tool', 'db_name', 'db_params', 'db_path']
|
||||
if ( !row.keySet().containsAll(expected_headers) ) exit 1, "[nf-core/taxprofiler] error: Invalid database input sheet - malformed column names. Please check input TSV. Column names should be: ${expected_keys.join(", ")}"
|
||||
|
||||
// valid tools specified// TIFNISIH LIST
|
||||
def expected_tools = [ "bracken", "centrifuge", "diamond", "kaiju", "kraken2", "malt", "metaphlan3" ]
|
||||
|
||||
// detect quotes in params
|
||||
if ( row.db_params.contains('"') ) exit 1, "[nf-core/taxprofiler] error: Invalid database db_params entry. No quotes allowed. Error in: ${row}"
|
||||
if ( row.db_params.contains("'") ) exit 1, "[nf-core/taxprofiler] error: Invalid database db_params entry. No quotes allowed. Error in: ${row}"
|
||||
|
||||
}
|
||||
|
||||
def create_db_channels(LinkedHashMap row) {
|
||||
|
@ -51,3 +72,5 @@ def create_db_channels(LinkedHashMap row) {
|
|||
|
||||
return array
|
||||
}
|
||||
|
||||
|
||||
|
|
|
@ -25,7 +25,7 @@ if ( params.input ) {
|
|||
exit 1, "Input samplesheet, or PEP config and base directory not specified"
|
||||
}
|
||||
|
||||
if (params.databases) { ch_databases = file(params.databases) } else { exit 1, 'Input database sheet not specified!' }
|
||||
if (params.databases) { ch_databases = file(params.databases, checkIfExists: true) } else { exit 1, 'Input database sheet not specified!' }
|
||||
|
||||
if (params.shortread_qc_mergepairs && params.run_malt ) log.warn "[nf-core/taxprofiler] MALT does not accept uncollapsed paired-reads. Pairs will be profiled as separate files."
|
||||
if (params.shortread_qc_includeunmerged && !params.shortread_qc_mergepairs) exit 1, "ERROR: [nf-core/taxprofiler] cannot include unmerged reads when merging is not turned on. Please specify --shortread_qc_mergepairs"
|
||||
|
|
Loading…
Reference in a new issue