mirror of
https://github.com/MillironX/taxprofiler.git
synced 2024-11-11 00:53:09 +00:00
commit
3fc7f1f0f8
24 changed files with 398 additions and 214 deletions
|
@ -12,7 +12,7 @@
|
|||
|
||||
params {
|
||||
config_profile_name = 'Test profile'
|
||||
config_profile_description = 'Minimal test dataset without performing any preprocessing nor profiling to check pipeline function. Useful when you only wish to test a single profiler without having to opt-out of all the others'
|
||||
config_profile_description = "Minimal test dataset without performing any preprocessing nor profiling to check pipeline function. Useful when you only wish to test a single profiler without having to 'opt-out' of all the others"
|
||||
|
||||
// Limit resources so that this can run on GitHub Actions
|
||||
max_cpus = 2
|
||||
|
|
233
modules.json
233
modules.json
|
@ -3,104 +3,141 @@
|
|||
"homePage": "https://github.com/nf-core/taxprofiler",
|
||||
"repos": {
|
||||
"nf-core/modules": {
|
||||
"adapterremoval": {
|
||||
"git_sha": "879d42c5e28661fe0a5e744c9e2c515868f9e08a"
|
||||
},
|
||||
"bbmap/bbduk": {
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"bowtie2/align": {
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"bowtie2/build": {
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"cat/fastq": {
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"centrifuge/centrifuge": {
|
||||
"git_sha": "d2726fcf75063960f06b36d2229a4c0966614108"
|
||||
},
|
||||
"centrifuge/kreport": {
|
||||
"git_sha": "734d0db6079a4aa43b6509b207e5d6feb35d4838"
|
||||
},
|
||||
"custom/dumpsoftwareversions": {
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"diamond/blastx": {
|
||||
"git_sha": "3531824af826c16cd252bc5aa82ae169b244ebaa"
|
||||
},
|
||||
"fastp": {
|
||||
"git_sha": "d0a1cbb703a130c19f6796c3fce24fbe7dfce789"
|
||||
},
|
||||
"fastqc": {
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"filtlong": {
|
||||
"git_sha": "957cb9b83668075f4af101fc99502908cca487e3"
|
||||
},
|
||||
"gunzip": {
|
||||
"git_sha": "9aadd9a6d3f5964476582319b3a1c54a3e3fe7c9"
|
||||
},
|
||||
"kaiju/kaiju": {
|
||||
"git_sha": "8856f127c58f6af479128be8b8df4d42e442ddbe"
|
||||
},
|
||||
"kaiju/kaiju2krona": {
|
||||
"git_sha": "2f0b19240430de6807b1232e6d9d0e8084e8a28f"
|
||||
},
|
||||
"kaiju/kaiju2table": {
|
||||
"git_sha": "538dbac98ba9c8f799536cd5a617195501439457"
|
||||
},
|
||||
"kraken2/kraken2": {
|
||||
"git_sha": "abe025677cdd805cc93032341ab19885473c1a07"
|
||||
},
|
||||
"krakentools/kreport2krona": {
|
||||
"git_sha": "8b2a473f586bed003e72d2b183acc43fc0ddc422"
|
||||
},
|
||||
"krona/ktimporttaxonomy": {
|
||||
"git_sha": "0e9fd9370ad1845870b8a9c63fcc47d999a1739e"
|
||||
},
|
||||
"krona/ktimporttext": {
|
||||
"git_sha": "cdefbec66999c0b49d8bfeea9d6f9d19056635a2"
|
||||
},
|
||||
"malt/run": {
|
||||
"git_sha": "be8d7b3293cac26cc63e4dbfb364deb8ed6ec7e5"
|
||||
},
|
||||
"megan/rma2info": {
|
||||
"git_sha": "2d38566eca4cc15142b2ffa7c11837569b39aece"
|
||||
},
|
||||
"metaphlan3": {
|
||||
"git_sha": "ed4dd1a928ebf4308efb720de878045f7773f8e2"
|
||||
},
|
||||
"minimap2/align": {
|
||||
"git_sha": "1a5a9e7b4009dcf34e6867dd1a5a1d9a718b027b"
|
||||
},
|
||||
"minimap2/index": {
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"motus/merge": {
|
||||
"git_sha": "b02e648c221e1da17cb589eefe297e61ec9e9c49"
|
||||
},
|
||||
"motus/profile": {
|
||||
"git_sha": "b6ed584443ad68ac41e6975994139454a4f23c18"
|
||||
},
|
||||
"multiqc": {
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"porechop": {
|
||||
"git_sha": "b78e19b9dae3671db2c7d4346fe04452c1debfab"
|
||||
},
|
||||
"prinseqplusplus": {
|
||||
"git_sha": "f1c5384c31e985591716afdd732cf8c2ae29d05b"
|
||||
},
|
||||
"samtools/bam2fq": {
|
||||
"git_sha": "5510ea39fe638594bc26ac34cadf4a84bf27d159"
|
||||
},
|
||||
"samtools/view": {
|
||||
"git_sha": "6b64f9cb6c3dd3577931cc3cd032d6fb730000ce"
|
||||
},
|
||||
"untar": {
|
||||
"git_sha": "e080f4c8acf5760039ed12ec1f206170f3f9a918"
|
||||
"git_url": "https://github.com/nf-core/modules.git",
|
||||
"modules": {
|
||||
"adapterremoval": {
|
||||
"branch": "master",
|
||||
"git_sha": "879d42c5e28661fe0a5e744c9e2c515868f9e08a"
|
||||
},
|
||||
"bbmap/bbduk": {
|
||||
"branch": "master",
|
||||
"git_sha": "848ee9a215d02d80be033bfa60881700f2bd914c"
|
||||
},
|
||||
"bowtie2/align": {
|
||||
"branch": "master",
|
||||
"git_sha": "848ee9a215d02d80be033bfa60881700f2bd914c"
|
||||
},
|
||||
"bowtie2/build": {
|
||||
"branch": "master",
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"cat/fastq": {
|
||||
"branch": "master",
|
||||
"git_sha": "b034029b59b1198075da8019074bc02051a6100e"
|
||||
},
|
||||
"centrifuge/centrifuge": {
|
||||
"branch": "master",
|
||||
"git_sha": "d2726fcf75063960f06b36d2229a4c0966614108"
|
||||
},
|
||||
"centrifuge/kreport": {
|
||||
"branch": "master",
|
||||
"git_sha": "734d0db6079a4aa43b6509b207e5d6feb35d4838"
|
||||
},
|
||||
"custom/dumpsoftwareversions": {
|
||||
"branch": "master",
|
||||
"git_sha": "5e7b1ef9a5a2d9258635bcbf70fcf37dacd1b247"
|
||||
},
|
||||
"diamond/blastx": {
|
||||
"branch": "master",
|
||||
"git_sha": "3531824af826c16cd252bc5aa82ae169b244ebaa"
|
||||
},
|
||||
"fastp": {
|
||||
"branch": "master",
|
||||
"git_sha": "7e8ad566883449e7939062b5e2bcf53fc1e0002f",
|
||||
"patch": "modules/nf-core/modules/fastp/fastp.diff"
|
||||
},
|
||||
"fastqc": {
|
||||
"branch": "master",
|
||||
"git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe"
|
||||
},
|
||||
"filtlong": {
|
||||
"branch": "master",
|
||||
"git_sha": "957cb9b83668075f4af101fc99502908cca487e3"
|
||||
},
|
||||
"gunzip": {
|
||||
"branch": "master",
|
||||
"git_sha": "fa37e0662690c4ec4260dae282fbce08777503e6"
|
||||
},
|
||||
"kaiju/kaiju": {
|
||||
"branch": "master",
|
||||
"git_sha": "8856f127c58f6af479128be8b8df4d42e442ddbe"
|
||||
},
|
||||
"kaiju/kaiju2krona": {
|
||||
"branch": "master",
|
||||
"git_sha": "2f0b19240430de6807b1232e6d9d0e8084e8a28f"
|
||||
},
|
||||
"kaiju/kaiju2table": {
|
||||
"branch": "master",
|
||||
"git_sha": "538dbac98ba9c8f799536cd5a617195501439457"
|
||||
},
|
||||
"kraken2/kraken2": {
|
||||
"branch": "master",
|
||||
"git_sha": "409a308ba46284d8ebb48c2c1befd6f6433db3f7"
|
||||
},
|
||||
"krakentools/kreport2krona": {
|
||||
"branch": "master",
|
||||
"git_sha": "233fa70811a03a4cecb2ece483b5c8396e2cee1d"
|
||||
},
|
||||
"krona/ktimporttaxonomy": {
|
||||
"branch": "master",
|
||||
"git_sha": "0e9fd9370ad1845870b8a9c63fcc47d999a1739e"
|
||||
},
|
||||
"krona/ktimporttext": {
|
||||
"branch": "master",
|
||||
"git_sha": "cdefbec66999c0b49d8bfeea9d6f9d19056635a2"
|
||||
},
|
||||
"malt/run": {
|
||||
"branch": "master",
|
||||
"git_sha": "be8d7b3293cac26cc63e4dbfb364deb8ed6ec7e5"
|
||||
},
|
||||
"megan/rma2info": {
|
||||
"branch": "master",
|
||||
"git_sha": "2d38566eca4cc15142b2ffa7c11837569b39aece"
|
||||
},
|
||||
"metaphlan3/metaphlan3": {
|
||||
"branch": "master",
|
||||
"git_sha": "940d7fe9d63962e0e2ba0987e2893fb0aff832e3"
|
||||
},
|
||||
"minimap2/align": {
|
||||
"branch": "master",
|
||||
"git_sha": "1a5a9e7b4009dcf34e6867dd1a5a1d9a718b027b"
|
||||
},
|
||||
"minimap2/index": {
|
||||
"branch": "master",
|
||||
"git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d"
|
||||
},
|
||||
"motus/merge": {
|
||||
"branch": "master",
|
||||
"git_sha": "b02e648c221e1da17cb589eefe297e61ec9e9c49"
|
||||
},
|
||||
"motus/profile": {
|
||||
"branch": "master",
|
||||
"git_sha": "b6ed584443ad68ac41e6975994139454a4f23c18"
|
||||
},
|
||||
"multiqc": {
|
||||
"branch": "master",
|
||||
"git_sha": "16eee433b87b303bda650131ac5a0b1ad725e166"
|
||||
},
|
||||
"porechop": {
|
||||
"branch": "master",
|
||||
"git_sha": "b78e19b9dae3671db2c7d4346fe04452c1debfab"
|
||||
},
|
||||
"prinseqplusplus": {
|
||||
"branch": "master",
|
||||
"git_sha": "f1c5384c31e985591716afdd732cf8c2ae29d05b"
|
||||
},
|
||||
"samtools/bam2fq": {
|
||||
"branch": "master",
|
||||
"git_sha": "5510ea39fe638594bc26ac34cadf4a84bf27d159"
|
||||
},
|
||||
"samtools/view": {
|
||||
"branch": "master",
|
||||
"git_sha": "5e7b1ef9a5a2d9258635bcbf70fcf37dacd1b247"
|
||||
},
|
||||
"untar": {
|
||||
"branch": "master",
|
||||
"git_sha": "5e7b1ef9a5a2d9258635bcbf70fcf37dacd1b247"
|
||||
}
|
||||
}
|
||||
}
|
||||
}
|
||||
|
|
1
modules/nf-core/modules/bbmap/bbduk/meta.yml
generated
1
modules/nf-core/modules/bbmap/bbduk/meta.yml
generated
|
@ -4,6 +4,7 @@ keywords:
|
|||
- trimming
|
||||
- adapter trimming
|
||||
- quality trimming
|
||||
- fastq
|
||||
tools:
|
||||
- bbmap:
|
||||
description: BBMap is a short read aligner, as well as various other bioinformatic tools.
|
||||
|
|
106
modules/nf-core/modules/bowtie2/align/main.nf
generated
106
modules/nf-core/modules/bowtie2/align/main.nf
generated
|
@ -1,77 +1,71 @@
|
|||
process BOWTIE2_ALIGN {
|
||||
tag "$meta.id"
|
||||
label 'process_high'
|
||||
label "process_high"
|
||||
|
||||
conda (params.enable_conda ? 'bioconda::bowtie2=2.4.4 bioconda::samtools=1.14 conda-forge::pigz=2.6' : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:4d235f41348a00533f18e47c9669f1ecb327f629-0' :
|
||||
'quay.io/biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:4d235f41348a00533f18e47c9669f1ecb327f629-0' }"
|
||||
conda (params.enable_conda ? "bioconda::bowtie2=2.4.4 bioconda::samtools=1.15.1 conda-forge::pigz=2.6" : null)
|
||||
container "${ workflow.containerEngine == "singularity" && !task.ext.singularity_pull_docker_container ?
|
||||
"https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:1744f68fe955578c63054b55309e05b41c37a80d-0" :
|
||||
"quay.io/biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:1744f68fe955578c63054b55309e05b41c37a80d-0" }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(reads)
|
||||
path index
|
||||
val save_unaligned
|
||||
val sort_bam
|
||||
|
||||
output:
|
||||
tuple val(meta), path('*.bam') , emit: bam
|
||||
tuple val(meta), path('*.log') , emit: log
|
||||
tuple val(meta), path('*fastq.gz'), emit: fastq, optional:true
|
||||
tuple val(meta), path("*.bam") , emit: bam
|
||||
tuple val(meta), path("*.log") , emit: log
|
||||
tuple val(meta), path("*fastq.gz"), emit: fastq, optional:true
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
||||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def args2 = task.ext.args2 ?: ''
|
||||
def args = task.ext.args ?: ""
|
||||
def args2 = task.ext.args2 ?: ""
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
|
||||
def unaligned = ""
|
||||
def reads_args = ""
|
||||
if (meta.single_end) {
|
||||
def unaligned = save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : ''
|
||||
"""
|
||||
INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
|
||||
bowtie2 \\
|
||||
-x \$INDEX \\
|
||||
-U $reads \\
|
||||
--threads $task.cpus \\
|
||||
$unaligned \\
|
||||
$args \\
|
||||
2> ${prefix}.bowtie2.log \\
|
||||
| samtools view -@ $task.cpus $args2 -bhS -o ${prefix}.bam -
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//')
|
||||
samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
|
||||
pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
|
||||
END_VERSIONS
|
||||
"""
|
||||
unaligned = save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : ""
|
||||
reads_args = "-U ${reads}"
|
||||
} else {
|
||||
def unaligned = save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : ''
|
||||
"""
|
||||
INDEX=`find -L ./ -name "*.rev.1.bt2" | sed 's/.rev.1.bt2//'`
|
||||
bowtie2 \\
|
||||
-x \$INDEX \\
|
||||
-1 ${reads[0]} \\
|
||||
-2 ${reads[1]} \\
|
||||
--threads $task.cpus \\
|
||||
$unaligned \\
|
||||
$args \\
|
||||
2> ${prefix}.bowtie2.log \\
|
||||
| samtools view -@ $task.cpus $args2 -bhS -o ${prefix}.bam -
|
||||
|
||||
if [ -f ${prefix}.unmapped.fastq.1.gz ]; then
|
||||
mv ${prefix}.unmapped.fastq.1.gz ${prefix}.unmapped_1.fastq.gz
|
||||
fi
|
||||
if [ -f ${prefix}.unmapped.fastq.2.gz ]; then
|
||||
mv ${prefix}.unmapped.fastq.2.gz ${prefix}.unmapped_2.fastq.gz
|
||||
fi
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//')
|
||||
samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
|
||||
pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
|
||||
END_VERSIONS
|
||||
"""
|
||||
unaligned = save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : ""
|
||||
reads_args = "-1 ${reads[0]} -2 ${reads[1]}"
|
||||
}
|
||||
|
||||
def samtools_command = sort_bam ? 'sort' : 'view'
|
||||
|
||||
"""
|
||||
INDEX=`find -L ./ -name "*.rev.1.bt2" | sed "s/.rev.1.bt2//"`
|
||||
[ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed "s/.rev.1.bt2l//"`
|
||||
[ -z "\$INDEX" ] && echo "Bowtie2 index files not found" 1>&2 && exit 1
|
||||
|
||||
bowtie2 \\
|
||||
-x \$INDEX \\
|
||||
$reads_args \\
|
||||
--threads $task.cpus \\
|
||||
$unaligned \\
|
||||
$args \\
|
||||
2> ${prefix}.bowtie2.log \\
|
||||
| samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.bam -
|
||||
|
||||
if [ -f ${prefix}.unmapped.fastq.1.gz ]; then
|
||||
mv ${prefix}.unmapped.fastq.1.gz ${prefix}.unmapped_1.fastq.gz
|
||||
fi
|
||||
|
||||
if [ -f ${prefix}.unmapped.fastq.2.gz ]; then
|
||||
mv ${prefix}.unmapped.fastq.2.gz ${prefix}.unmapped_2.fastq.gz
|
||||
fi
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//')
|
||||
samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
|
||||
pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' )
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
|
|
11
modules/nf-core/modules/bowtie2/align/meta.yml
generated
11
modules/nf-core/modules/bowtie2/align/meta.yml
generated
|
@ -2,7 +2,9 @@ name: bowtie2_align
|
|||
description: Align reads to a reference genome using bowtie2
|
||||
keywords:
|
||||
- align
|
||||
- map
|
||||
- fasta
|
||||
- fastq
|
||||
- genome
|
||||
- reference
|
||||
tools:
|
||||
|
@ -29,6 +31,15 @@ input:
|
|||
type: file
|
||||
description: Bowtie2 genome index files
|
||||
pattern: "*.ebwt"
|
||||
- save_unaligned:
|
||||
type: boolean
|
||||
description: |
|
||||
Save reads that do not map to the reference (true) or discard them (false)
|
||||
(default: false)
|
||||
- sort_bam:
|
||||
type: boolean
|
||||
description: use samtools sort (true) or samtools view (false)
|
||||
pattern: "true or false"
|
||||
output:
|
||||
- bam:
|
||||
type: file
|
||||
|
|
33
modules/nf-core/modules/cat/fastq/main.nf
generated
33
modules/nf-core/modules/cat/fastq/main.nf
generated
|
@ -4,8 +4,8 @@ process CAT_FASTQ {
|
|||
|
||||
conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
|
||||
'biocontainers/biocontainers:v1.2.0_cv1' }"
|
||||
'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
|
||||
'ubuntu:20.04' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(reads, stageAs: "input*/*")
|
||||
|
@ -48,4 +48,33 @@ process CAT_FASTQ {
|
|||
"""
|
||||
}
|
||||
}
|
||||
|
||||
stub:
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def readList = reads.collect{ it.toString() }
|
||||
if (meta.single_end) {
|
||||
if (readList.size > 1) {
|
||||
"""
|
||||
touch ${prefix}.merged.fastq.gz
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
} else {
|
||||
if (readList.size > 2) {
|
||||
"""
|
||||
touch ${prefix}_1.merged.fastq.gz
|
||||
touch ${prefix}_2.merged.fastq.gz
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*coreutils) //; s/ .*\$//')
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
}
|
||||
|
||||
}
|
||||
|
|
|
@ -1,11 +1,11 @@
|
|||
process CUSTOM_DUMPSOFTWAREVERSIONS {
|
||||
label 'process_low'
|
||||
label 'process_single'
|
||||
|
||||
// Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container
|
||||
conda (params.enable_conda ? "bioconda::multiqc=1.11" : null)
|
||||
conda (params.enable_conda ? 'bioconda::multiqc=1.13a' : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' :
|
||||
'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/multiqc:1.13a--pyhdfd78af_1' :
|
||||
'quay.io/biocontainers/multiqc:1.13a--pyhdfd78af_1' }"
|
||||
|
||||
input:
|
||||
path versions
|
||||
|
|
|
@ -1,10 +1,9 @@
|
|||
#!/usr/bin/env python
|
||||
|
||||
import yaml
|
||||
import platform
|
||||
from textwrap import dedent
|
||||
|
||||
import yaml
|
||||
|
||||
|
||||
def _make_versions_html(versions):
|
||||
html = [
|
||||
|
@ -59,12 +58,11 @@ versions_by_module = {}
|
|||
for process, process_versions in versions_by_process.items():
|
||||
module = process.split(":")[-1]
|
||||
try:
|
||||
if versions_by_module[module] != process_versions:
|
||||
raise AssertionError(
|
||||
"We assume that software versions are the same between all modules. "
|
||||
"If you see this error-message it means you discovered an edge-case "
|
||||
"and should open an issue in nf-core/tools. "
|
||||
)
|
||||
assert versions_by_module[module] == process_versions, (
|
||||
"We assume that software versions are the same between all modules. "
|
||||
"If you see this error-message it means you discovered an edge-case "
|
||||
"and should open an issue in nf-core/tools. "
|
||||
)
|
||||
except KeyError:
|
||||
versions_by_module[module] = process_versions
|
||||
|
||||
|
|
33
modules/nf-core/modules/fastp/fastp.diff
generated
Normal file
33
modules/nf-core/modules/fastp/fastp.diff
generated
Normal file
|
@ -0,0 +1,33 @@
|
|||
Changes in module 'nf-core/modules/fastp'
|
||||
--- modules/nf-core/modules/fastp/main.nf
|
||||
+++ modules/nf-core/modules/fastp/main.nf
|
||||
@@ -33,9 +33,8 @@
|
||||
def fail_fastq = save_trimmed_fail ? "--failed_out ${prefix}.fail.fastq.gz" : ''
|
||||
"""
|
||||
[ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz
|
||||
- cat ${prefix}.fastq.gz \\
|
||||
- | fastp \\
|
||||
- --stdin \\
|
||||
+
|
||||
+ fastp \\
|
||||
--stdout \\
|
||||
--in1 ${prefix}.fastq.gz \\
|
||||
--thread $task.cpus \\
|
||||
@@ -45,6 +44,7 @@
|
||||
$args \\
|
||||
2> ${prefix}.fastp.log \\
|
||||
| gzip -c > ${prefix}.fastp.fastq.gz
|
||||
+
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g")
|
||||
@@ -69,6 +69,7 @@
|
||||
--detect_adapter_for_pe \\
|
||||
$args \\
|
||||
2> ${prefix}.fastp.log
|
||||
+
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
||||
************************************************************
|
21
modules/nf-core/modules/fastp/main.nf
generated
21
modules/nf-core/modules/fastp/main.nf
generated
|
@ -13,7 +13,7 @@ process FASTP {
|
|||
val save_merged
|
||||
|
||||
output:
|
||||
tuple val(meta), path('*.trim.fastq.gz') , optional:true, emit: reads
|
||||
tuple val(meta), path('*.fastp.fastq.gz') , optional:true, emit: reads
|
||||
tuple val(meta), path('*.json') , emit: json
|
||||
tuple val(meta), path('*.html') , emit: html
|
||||
tuple val(meta), path('*.log') , emit: log
|
||||
|
@ -28,19 +28,23 @@ process FASTP {
|
|||
def args = task.ext.args ?: ''
|
||||
// Added soft-links to original fastqs for consistent naming in MultiQC
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
// Use single ended for interleaved. Add --interleaved_in in config.
|
||||
if (meta.single_end) {
|
||||
def fail_fastq = save_trimmed_fail ? "--failed_out ${prefix}.fail.fastq.gz" : ''
|
||||
"""
|
||||
[ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz
|
||||
[ ! -f ${prefix}.fastq.gz ] && ln -sf $reads ${prefix}.fastq.gz
|
||||
|
||||
fastp \\
|
||||
--stdout \\
|
||||
--in1 ${prefix}.fastq.gz \\
|
||||
--out1 ${prefix}.trim.fastq.gz \\
|
||||
--thread $task.cpus \\
|
||||
--json ${prefix}.fastp.json \\
|
||||
--html ${prefix}.fastp.html \\
|
||||
$fail_fastq \\
|
||||
$args \\
|
||||
2> ${prefix}.fastp.log
|
||||
2> ${prefix}.fastp.log \\
|
||||
| gzip -c > ${prefix}.fastp.fastq.gz
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g")
|
||||
|
@ -50,13 +54,13 @@ process FASTP {
|
|||
def fail_fastq = save_trimmed_fail ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : ''
|
||||
def merge_fastq = save_merged ? "-m --merged_out ${prefix}.merged.fastq.gz" : ''
|
||||
"""
|
||||
[ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz
|
||||
[ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz
|
||||
[ ! -f ${prefix}_1.fastq.gz ] && ln -sf ${reads[0]} ${prefix}_1.fastq.gz
|
||||
[ ! -f ${prefix}_2.fastq.gz ] && ln -sf ${reads[1]} ${prefix}_2.fastq.gz
|
||||
fastp \\
|
||||
--in1 ${prefix}_1.fastq.gz \\
|
||||
--in2 ${prefix}_2.fastq.gz \\
|
||||
--out1 ${prefix}_1.trim.fastq.gz \\
|
||||
--out2 ${prefix}_2.trim.fastq.gz \\
|
||||
--out1 ${prefix}_1.fastp.fastq.gz \\
|
||||
--out2 ${prefix}_2.fastp.fastq.gz \\
|
||||
--json ${prefix}.fastp.json \\
|
||||
--html ${prefix}.fastp.html \\
|
||||
$fail_fastq \\
|
||||
|
@ -66,6 +70,7 @@ process FASTP {
|
|||
$args \\
|
||||
2> ${prefix}.fastp.log
|
||||
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
fastp: \$(fastp --version 2>&1 | sed -e "s/fastp //g")
|
||||
|
|
4
modules/nf-core/modules/fastp/meta.yml
generated
4
modules/nf-core/modules/fastp/meta.yml
generated
|
@ -15,7 +15,7 @@ input:
|
|||
- meta:
|
||||
type: map
|
||||
description: |
|
||||
Groovy Map containing sample information
|
||||
Groovy Map containing sample information. Use 'single_end: true' to specify single ended or interleaved FASTQs. Use 'single_end: false' for paired-end reads.
|
||||
e.g. [ id:'test', single_end:false ]
|
||||
- reads:
|
||||
type: file
|
||||
|
@ -38,7 +38,7 @@ output:
|
|||
- reads:
|
||||
type: file
|
||||
description: The trimmed/modified/unmerged fastq reads
|
||||
pattern: "*trim.fastq.gz"
|
||||
pattern: "*fastp.fastq.gz"
|
||||
- json:
|
||||
type: file
|
||||
description: Results in JSON format
|
||||
|
|
12
modules/nf-core/modules/fastqc/main.nf
generated
12
modules/nf-core/modules/fastqc/main.nf
generated
|
@ -44,4 +44,16 @@ process FASTQC {
|
|||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
|
||||
stub:
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
"""
|
||||
touch ${prefix}.html
|
||||
touch ${prefix}.zip
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" )
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
|
|
10
modules/nf-core/modules/gunzip/main.nf
generated
10
modules/nf-core/modules/gunzip/main.nf
generated
|
@ -31,4 +31,14 @@ process GUNZIP {
|
|||
gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
|
||||
END_VERSIONS
|
||||
"""
|
||||
|
||||
stub:
|
||||
gunzip = archive.toString() - '.gz'
|
||||
"""
|
||||
touch $gunzip
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
gunzip: \$(echo \$(gunzip --version 2>&1) | sed 's/^.*(gzip) //; s/ Copyright.*\$//')
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
|
|
22
modules/nf-core/modules/kraken2/kraken2/main.nf
generated
22
modules/nf-core/modules/kraken2/kraken2/main.nf
generated
|
@ -14,11 +14,11 @@ process KRAKEN2_KRAKEN2 {
|
|||
val save_reads_assignment
|
||||
|
||||
output:
|
||||
tuple val(meta), path('*classified*') , optional:true, emit: classified_reads_fastq
|
||||
tuple val(meta), path('*unclassified*') , optional:true, emit: unclassified_reads_fastq
|
||||
tuple val(meta), path('*classifiedreads*'), optional:true, emit: classified_reads_assignment
|
||||
tuple val(meta), path('*report.txt') , emit: report
|
||||
path "versions.yml" , emit: versions
|
||||
tuple val(meta), path('*.classified{.,_}*') , optional:true, emit: classified_reads_fastq
|
||||
tuple val(meta), path('*.unclassified{.,_}*') , optional:true, emit: unclassified_reads_fastq
|
||||
tuple val(meta), path('*classifiedreads.txt') , optional:true, emit: classified_reads_assignment
|
||||
tuple val(meta), path('*report.txt') , emit: report
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
@ -29,9 +29,9 @@ process KRAKEN2_KRAKEN2 {
|
|||
def paired = meta.single_end ? "" : "--paired"
|
||||
def classified = meta.single_end ? "${prefix}.classified.fastq" : "${prefix}.classified#.fastq"
|
||||
def unclassified = meta.single_end ? "${prefix}.unclassified.fastq" : "${prefix}.unclassified#.fastq"
|
||||
def classified_command = save_output_fastqs ? "--classified-out ${classified}" : ""
|
||||
def unclassified_command = save_output_fastqs ? "--unclassified-out ${unclassified}" : ""
|
||||
def readclassification_command = save_reads_assignment ? "--output ${prefix}.kraken2.classifiedreads.txt" : ""
|
||||
def classified_option = save_output_fastqs ? "--classified-out ${classified}" : ""
|
||||
def unclassified_option = save_output_fastqs ? "--unclassified-out ${unclassified}" : ""
|
||||
def readclassification_option = save_reads_assignment ? "--output ${prefix}.kraken2.classifiedreads.txt" : ""
|
||||
def compress_reads_command = save_output_fastqs ? "pigz -p $task.cpus *.fastq" : ""
|
||||
|
||||
"""
|
||||
|
@ -40,9 +40,9 @@ process KRAKEN2_KRAKEN2 {
|
|||
--threads $task.cpus \\
|
||||
--report ${prefix}.kraken2.report.txt \\
|
||||
--gzip-compressed \\
|
||||
$unclassified_command \\
|
||||
$classified_command \\
|
||||
$readclassification_command \\
|
||||
$unclassified_option \\
|
||||
$classified_option \\
|
||||
$readclassification_option \\
|
||||
$paired \\
|
||||
$args \\
|
||||
$reads
|
||||
|
|
|
@ -1,9 +1,8 @@
|
|||
def VERSION = '1.2' // Version information not provided by tool on CLI
|
||||
|
||||
process KRAKENTOOLS_KREPORT2KRONA {
|
||||
tag "$meta.id"
|
||||
label 'process_low'
|
||||
|
||||
// WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions.
|
||||
conda (params.enable_conda ? "bioconda::krakentools=1.2" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/krakentools:1.2--pyh5e36f6f_0':
|
||||
|
@ -22,6 +21,7 @@ process KRAKENTOOLS_KREPORT2KRONA {
|
|||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def VERSION = '1.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
|
||||
"""
|
||||
kreport2krona.py \\
|
||||
-r ${kreport} \\
|
||||
|
|
|
@ -28,15 +28,18 @@ process METAPHLAN3 {
|
|||
def bowtie2_out = "$input_type" == "--input_type bowtie2out" || "$input_type" == "--input_type sam" ? '' : "--bowtie2out ${prefix}.bowtie2out.txt"
|
||||
|
||||
"""
|
||||
BT2_DB=`find -L "${metaphlan_db}" -name "*rev.1.bt2" -exec dirname {} \\;`
|
||||
|
||||
metaphlan \\
|
||||
--nproc $task.cpus \\
|
||||
$input_type \\
|
||||
$input_data \\
|
||||
$args \\
|
||||
$bowtie2_out \\
|
||||
--bowtie2db ${metaphlan_db} \\
|
||||
--bowtie2db \$BT2_DB \\
|
||||
--biom ${prefix}.biom \\
|
||||
--output_file ${prefix}_profile.txt
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
metaphlan3: \$(metaphlan --version 2>&1 | awk '{print \$3}')
|
|
@ -24,6 +24,12 @@ input:
|
|||
type: file
|
||||
description: Metaphlan 3.0 can classify the metagenome from a variety of input data types, including FASTQ files (single-end and paired-end), FASTA, bowtie2-produced SAM files (produced from alignments to the MetaPHlAn marker database) and intermediate bowtie2 alignment files (bowtie2out)
|
||||
pattern: "*.{fastq.gz, fasta, fasta.gz, sam, bowtie2out.txt}"
|
||||
- metaphlan_db:
|
||||
type: file
|
||||
description: |
|
||||
Directory containing pre-downloaded and uncompressed MetaPhlAn3 database downloaded from: http://cmprod1.cibio.unitn.it/biobakery3/metaphlan_databases/.
|
||||
Note that you will also need to specify `--index` and the database version name (e.g. 'mpa_v31_CHOCOPhlAn_201901') in your module.conf ext.args for METAPHLAN3_METAPHLAN3!
|
||||
pattern: "*/"
|
||||
|
||||
output:
|
||||
- meta:
|
29
modules/nf-core/modules/multiqc/main.nf
generated
29
modules/nf-core/modules/multiqc/main.nf
generated
|
@ -1,13 +1,15 @@
|
|||
process MULTIQC {
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? 'bioconda::multiqc=1.12' : null)
|
||||
conda (params.enable_conda ? 'bioconda::multiqc=1.13a' : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/multiqc:1.12--pyhdfd78af_0' :
|
||||
'quay.io/biocontainers/multiqc:1.12--pyhdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/multiqc:1.13a--pyhdfd78af_1' :
|
||||
'quay.io/biocontainers/multiqc:1.13a--pyhdfd78af_1' }"
|
||||
|
||||
input:
|
||||
path multiqc_files
|
||||
path multiqc_files, stageAs: "?/*"
|
||||
path(multiqc_config)
|
||||
path(multiqc_logo)
|
||||
|
||||
output:
|
||||
path "*multiqc_report.html", emit: report
|
||||
|
@ -20,8 +22,25 @@ process MULTIQC {
|
|||
|
||||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def config = multiqc_config ? "--config $multiqc_config" : ''
|
||||
"""
|
||||
multiqc -f $args .
|
||||
multiqc \\
|
||||
--force \\
|
||||
$config \\
|
||||
$args \\
|
||||
.
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" )
|
||||
END_VERSIONS
|
||||
"""
|
||||
|
||||
stub:
|
||||
"""
|
||||
touch multiqc_data
|
||||
touch multiqc_plots
|
||||
touch multiqc_report.html
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
11
modules/nf-core/modules/multiqc/meta.yml
generated
11
modules/nf-core/modules/multiqc/meta.yml
generated
|
@ -12,11 +12,21 @@ tools:
|
|||
homepage: https://multiqc.info/
|
||||
documentation: https://multiqc.info/docs/
|
||||
licence: ["GPL-3.0-or-later"]
|
||||
|
||||
input:
|
||||
- multiqc_files:
|
||||
type: file
|
||||
description: |
|
||||
List of reports / files recognised by MultiQC, for example the html and zip output of FastQC
|
||||
- multiqc_config:
|
||||
type: file
|
||||
description: Optional config yml for MultiQC
|
||||
pattern: "*.{yml,yaml}"
|
||||
- multiqc_logo:
|
||||
type: file
|
||||
description: Optional logo file for MultiQC
|
||||
pattern: "*.{png}"
|
||||
|
||||
output:
|
||||
- report:
|
||||
type: file
|
||||
|
@ -38,3 +48,4 @@ authors:
|
|||
- "@abhi18av"
|
||||
- "@bunop"
|
||||
- "@drpatelh"
|
||||
- "@jfy133"
|
||||
|
|
2
modules/nf-core/modules/samtools/view/main.nf
generated
2
modules/nf-core/modules/samtools/view/main.nf
generated
|
@ -1,6 +1,6 @@
|
|||
process SAMTOOLS_VIEW {
|
||||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
|
|
27
modules/nf-core/modules/untar/main.nf
generated
27
modules/nf-core/modules/untar/main.nf
generated
|
@ -1,11 +1,11 @@
|
|||
process UNTAR {
|
||||
tag "$archive"
|
||||
label 'process_low'
|
||||
label 'process_single'
|
||||
|
||||
conda (params.enable_conda ? "conda-forge::tar=1.32" : null)
|
||||
conda (params.enable_conda ? "conda-forge::sed=4.7" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv1/biocontainers_v1.2.0_cv1.img' :
|
||||
'biocontainers/biocontainers:v1.2.0_cv1' }"
|
||||
'https://depot.galaxyproject.org/singularity/ubuntu:20.04' :
|
||||
'ubuntu:20.04' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(archive)
|
||||
|
@ -21,12 +21,29 @@ process UNTAR {
|
|||
def args = task.ext.args ?: ''
|
||||
def args2 = task.ext.args2 ?: ''
|
||||
untar = archive.toString() - '.tar.gz'
|
||||
|
||||
"""
|
||||
mkdir output
|
||||
|
||||
tar \\
|
||||
-C output --strip-components 1 \\
|
||||
-xzvf \\
|
||||
$args \\
|
||||
$archive \\
|
||||
$args2 \\
|
||||
$args2
|
||||
|
||||
mv output ${untar}
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//')
|
||||
END_VERSIONS
|
||||
"""
|
||||
|
||||
stub:
|
||||
untar = archive.toString() - '.tar.gz'
|
||||
"""
|
||||
touch $untar
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
@ -7,7 +7,7 @@ include { MEGAN_RMA2INFO as MEGAN_RMA2INFO_TSV } from '../../modules/nf-core/mo
|
|||
include { KRAKEN2_KRAKEN2 } from '../../modules/nf-core/modules/kraken2/kraken2/main'
|
||||
include { CENTRIFUGE_CENTRIFUGE } from '../../modules/nf-core/modules/centrifuge/centrifuge/main'
|
||||
include { CENTRIFUGE_KREPORT } from '../../modules/nf-core/modules/centrifuge/kreport/main'
|
||||
include { METAPHLAN3 } from '../../modules/nf-core/modules/metaphlan3/main'
|
||||
include { METAPHLAN3 } from '../../modules/nf-core/modules/metaphlan3/metaphlan3/main'
|
||||
include { KAIJU_KAIJU } from '../../modules/nf-core/modules/kaiju/kaiju/main'
|
||||
include { DIAMOND_BLASTX } from '../../modules/nf-core/modules/diamond/blastx/main'
|
||||
include { MOTUS_PROFILE } from '../../modules/nf-core/modules/motus/profile/main'
|
||||
|
|
|
@ -22,7 +22,7 @@ workflow SHORTREAD_HOSTREMOVAL {
|
|||
ch_bowtie2_index = index.first()
|
||||
}
|
||||
|
||||
BOWTIE2_ALIGN ( reads, ch_bowtie2_index, true )
|
||||
BOWTIE2_ALIGN ( reads, ch_bowtie2_index, true, false )
|
||||
ch_versions = ch_versions.mix( BOWTIE2_ALIGN.out.versions.first() )
|
||||
ch_multiqc_files = ch_multiqc_files.mix( BOWTIE2_ALIGN.out.log )
|
||||
|
||||
|
|
|
@ -42,8 +42,7 @@ if (params.run_malt && params.run_krona && !params.krona_taxonomy_directory) log
|
|||
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||
*/
|
||||
|
||||
ch_multiqc_config = file("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
|
||||
ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
|
||||
ch_multiqc_config = params.multiqc_config ? file( params.multiqc_config, checkIfExists: true ) : file("$projectDir/assets/multiqc_config.yml", checkIfExists: true)
|
||||
|
||||
/*
|
||||
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||
|
@ -93,7 +92,7 @@ def multiqc_report = []
|
|||
workflow TAXPROFILER {
|
||||
|
||||
ch_versions = Channel.empty()
|
||||
ch_taxprofiler_logo = Channel.fromPath("$projectDir/docs/images/nf-core-taxprofiler_logo_custom_light.png")
|
||||
ch_multiqc_logo= Channel.fromPath("$projectDir/docs/images/nf-core-taxprofiler_logo_custom_light.png")
|
||||
|
||||
/*
|
||||
SUBWORKFLOW: Read in samplesheet, validate and stage input files
|
||||
|
@ -241,14 +240,11 @@ workflow TAXPROFILER {
|
|||
ch_workflow_summary = Channel.value(workflow_summary)
|
||||
|
||||
ch_multiqc_files = Channel.empty()
|
||||
ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config))
|
||||
ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([]))
|
||||
|
||||
ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'))
|
||||
ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect())
|
||||
ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([]))
|
||||
|
||||
ch_multiqc_files = ch_multiqc_files.mix(ch_taxprofiler_logo.ifEmpty([]))
|
||||
|
||||
if (params.perform_shortread_qc) {
|
||||
ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_PREPROCESSING.out.mqc.collect{it[1]}.ifEmpty([]) )
|
||||
}
|
||||
|
@ -273,7 +269,9 @@ workflow TAXPROFILER {
|
|||
|
||||
// TODO create multiQC module for metaphlan
|
||||
MULTIQC (
|
||||
ch_multiqc_files.collect()
|
||||
ch_multiqc_files.collect(),
|
||||
ch_multiqc_config,
|
||||
ch_multiqc_logo
|
||||
)
|
||||
multiqc_report = MULTIQC.out.report.toList()
|
||||
ch_versions = ch_versions.mix(MULTIQC.out.versions)
|
||||
|
|
Loading…
Reference in a new issue