From 58dffc0c5d4d710950dc2fe43555f353ce919f0a Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Tue, 11 Oct 2022 12:36:48 +0200 Subject: [PATCH 001/132] Add KrakenUniq and Filtlong in documentation --- README.md | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/README.md b/README.md index 11eb9a3..1537a6f 100644 --- a/README.md +++ b/README.md @@ -32,8 +32,8 @@ On release, automated continuous integration tests run the pipeline on a full-si 1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 2. Performs optional read pre-processing - - Adapter clipping and merging (short read: [fastp](https://github.com/OpenGene/fastp), [AdapterRemoval2](https://github.com/MikkelSchubert/adapterremoval); long read: [porechop](https://github.com/rrwick/Porechop)) - - Low complexity filtering ([bbduk](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/), [PRINSEQ++](https://github.com/Adrian-Cantu/PRINSEQ-plus-plus)) + - Adapter clipping and merging (short-read: [fastp](https://github.com/OpenGene/fastp), [AdapterRemoval2](https://github.com/MikkelSchubert/adapterremoval); long-read: [porechop](https://github.com/rrwick/Porechop)) + - Low complexity filtering (short-read: [bbduk](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/), [PRINSEQ++](https://github.com/Adrian-Cantu/PRINSEQ-plus-plus); long-read: [Filtlong](https://github.com/rrwick/Filtlong)) - Host read removal ([BowTie2](http://bowtie-bio.sourceforge.net/bowtie2/)) - Run merging 3. Performs taxonomic profiling using one or more of: @@ -44,7 +44,7 @@ On release, automated continuous integration tests run the pipeline on a full-si - [Centrifuge](https://ccb.jhu.edu/software/centrifuge/) - [Kaiju](https://kaiju.binf.ku.dk/) - [mOTUs](https://motu-tool.org/) - - [MetaMaps](https://github.com/DiltheyLab/MetaMaps) + - [KrakenUniq](https://github.com/fbreitwieser/krakenuniq) 4. Perform optional post-processing with: - [bracken](https://ccb.jhu.edu/software/bracken/) 5. Standardises output tables From dea45c66c24bc1bed23c1a6ea964e8d78dacd921 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Tue, 11 Oct 2022 14:59:48 +0200 Subject: [PATCH 002/132] Add Krona description --- README.md | 1 + 1 file changed, 1 insertion(+) diff --git a/README.md b/README.md index 1537a6f..9385b33 100644 --- a/README.md +++ b/README.md @@ -49,6 +49,7 @@ On release, automated continuous integration tests run the pipeline on a full-si - [bracken](https://ccb.jhu.edu/software/bracken/) 5. Standardises output tables 6. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +7. Plotting Kraken2, Centrifuge, Kaiju and MALT results ([`Krona`](https://hpc.nih.gov/apps/kronatools.html)) ## Quick Start From 2423c9d87cef7c4c7588f21abdc5d46a85c18e66 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Tue, 11 Oct 2022 15:02:01 +0200 Subject: [PATCH 003/132] Add headers --- docs/usage.md | 1 + 1 file changed, 1 insertion(+) diff --git a/docs/usage.md b/docs/usage.md index 8b8b588..4f4c1ac 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -47,6 +47,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data, as well as long-read FASTA files may look something like the one below. This is for 6 samples, where `2612` has been sequenced twice. ```console +sample,run_accession,instrument_platform,fastq_1,fastq_2,fasta 2611,ERR5766174,ILLUMINA,,,///fasta/ERX5474930_ERR5766174_1.fa.gz 2612,ERR5766176,ILLUMINA,///fastq/ERX5474932_ERR5766176_1.fastq.gz,///fastq/ERX5474932_ERR5766176_2.fastq.gz, 2612,ERR5766180,ILLUMINA,///fastq/ERX5474936_ERR5766180_1.fastq.gz,, From f24344c168f6d98a8f58c38631557f5b9885f0e7 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Tue, 11 Oct 2022 16:30:42 +0200 Subject: [PATCH 004/132] Update README.md --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 9385b33..73dfe93 100644 --- a/README.md +++ b/README.md @@ -33,7 +33,7 @@ On release, automated continuous integration tests run the pipeline on a full-si 1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 2. Performs optional read pre-processing - Adapter clipping and merging (short-read: [fastp](https://github.com/OpenGene/fastp), [AdapterRemoval2](https://github.com/MikkelSchubert/adapterremoval); long-read: [porechop](https://github.com/rrwick/Porechop)) - - Low complexity filtering (short-read: [bbduk](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/), [PRINSEQ++](https://github.com/Adrian-Cantu/PRINSEQ-plus-plus); long-read: [Filtlong](https://github.com/rrwick/Filtlong)) + - Low complexity and quality filtering (short-read: [bbduk](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/), [PRINSEQ++](https://github.com/Adrian-Cantu/PRINSEQ-plus-plus); long-read: [Filtlong](https://github.com/rrwick/Filtlong)) - Host read removal ([BowTie2](http://bowtie-bio.sourceforge.net/bowtie2/)) - Run merging 3. Performs taxonomic profiling using one or more of: From 35b7fc26f7ab0711c6fcebe45806d807ed6d8d1e Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Tue, 11 Oct 2022 17:01:08 +0200 Subject: [PATCH 005/132] [skip ci] --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 73dfe93..8d45ff8 100644 --- a/README.md +++ b/README.md @@ -53,7 +53,7 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Quick Start -1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`) +1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`). 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_. From bd56bc0ed629e5ecb012cd39c98b0f5aa3663335 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Wed, 12 Oct 2022 11:10:53 +0200 Subject: [PATCH 006/132] Add minimap2 and update contributors --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 8d45ff8..2bd45a9 100644 --- a/README.md +++ b/README.md @@ -34,7 +34,7 @@ On release, automated continuous integration tests run the pipeline on a full-si 2. Performs optional read pre-processing - Adapter clipping and merging (short-read: [fastp](https://github.com/OpenGene/fastp), [AdapterRemoval2](https://github.com/MikkelSchubert/adapterremoval); long-read: [porechop](https://github.com/rrwick/Porechop)) - Low complexity and quality filtering (short-read: [bbduk](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/), [PRINSEQ++](https://github.com/Adrian-Cantu/PRINSEQ-plus-plus); long-read: [Filtlong](https://github.com/rrwick/Filtlong)) - - Host read removal ([BowTie2](http://bowtie-bio.sourceforge.net/bowtie2/)) + - Host read removal (short-read: [BowTie2](http://bowtie-bio.sourceforge.net/bowtie2/); long-read: [Minimap2](https://github.com/lh3/minimap2)) - Run merging 3. Performs taxonomic profiling using one or more of: - [Kraken2](https://ccb.jhu.edu/software/kraken2/) @@ -88,7 +88,7 @@ nf-core/taxprofiler was originally written by nf-core community. We thank the following people for their extensive assistance in the development of this pipeline: -[James A. Fellows Yates](https://github.com/jfy133), [Moritz Beber](https://github.com/Midnighter), [Lauri Mesilaakso](https://github.com/ljmesi), [Sofia Stamouli](https://github.com/sofsam), [Maxime Borry](https://github.com/maxibor). +[James A. Fellows Yates](https://github.com/jfy133), [Moritz Beber](https://github.com/Midnighter), [Lauri Mesilaakso](https://github.com/ljmesi), [Sofia Stamouli](https://github.com/sofsam), [Maxime Borry](https://github.com/maxibor),[Thomas A. Christensen II](https://github.com/MillironX), [Jianhong Ou](https://github.com/jianhong), [Rafal Stepien](https://github.com/rafalstepien), [Mahwash Jamy](https://github.com/mjamy). ## Contributions and Support From 4352d35937560e977904f1e9154691b76aff41f8 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 14 Oct 2022 10:52:17 +0200 Subject: [PATCH 007/132] chore: install Bracken module --- modules.json | 4 +++ modules/nf-core/bracken/bracken/main.nf | 45 ++++++++++++++++++++++++ modules/nf-core/bracken/bracken/meta.yml | 45 ++++++++++++++++++++++++ 3 files changed, 94 insertions(+) create mode 100644 modules/nf-core/bracken/bracken/main.nf create mode 100644 modules/nf-core/bracken/bracken/meta.yml diff --git a/modules.json b/modules.json index 10d6c74..8f89a11 100644 --- a/modules.json +++ b/modules.json @@ -21,6 +21,10 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" }, + "bracken/bracken": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, "cat/fastq": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" diff --git a/modules/nf-core/bracken/bracken/main.nf b/modules/nf-core/bracken/bracken/main.nf new file mode 100644 index 0000000..5e08418 --- /dev/null +++ b/modules/nf-core/bracken/bracken/main.nf @@ -0,0 +1,45 @@ +process BRACKEN_BRACKEN { + tag "$meta.id" + label 'process_low' + + // WARN: Version information not provided by tool on CLI. + // Please update version string below when bumping container versions. + conda (params.enable_conda ? "bioconda::bracken=2.7" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bracken:2.7--py39hc16433a_0': + 'quay.io/biocontainers/bracken:2.7--py39hc16433a_0' }" + + input: + tuple val(meta), path(kraken_report) + path database + + output: + tuple val(meta), path(bracken_report), emit: reports + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def threshold = meta.threshold ?: 10 + def taxonomic_level = meta.taxonomic_level ?: 'S' + def read_length = meta.read_length ?: 150 + def args = task.ext.args ?: "-l ${taxonomic_level} -t ${threshold} -r ${read_length}" + def prefix = task.ext.prefix ?: "${meta.id}" + bracken_report = "${prefix}_${taxonomic_level}.tsv" + // WARN: Version information not provided by tool on CLI. + // Please update version string below when bumping container versions. + def VERSION = '2.7' + """ + bracken \\ + ${args} \\ + -d '${database}' \\ + -i '${kraken_report}' \\ + -o '${bracken_report}' + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bracken: ${VERSION} + END_VERSIONS + """ +} diff --git a/modules/nf-core/bracken/bracken/meta.yml b/modules/nf-core/bracken/bracken/meta.yml new file mode 100644 index 0000000..1931161 --- /dev/null +++ b/modules/nf-core/bracken/bracken/meta.yml @@ -0,0 +1,45 @@ +name: bracken_bracken +description: Re-estimate taxonomic abundance of metagenomic samples analyzed by kraken. +keywords: + - sort +tools: + - bracken: + description: Bracken (Bayesian Reestimation of Abundance with KrakEN) is a highly accurate statistical method that computes the abundance of species in DNA sequences from a metagenomics sample. + homepage: https://ccb.jhu.edu/software/bracken/ + documentation: https://ccb.jhu.edu/software/bracken/index.shtml?t=manual + tool_dev_url: https://github.com/jenniferlu717/Bracken + doi: "10.7717/peerj-cs.104" + licence: ["GPL v3"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - kraken_report: + type: file + description: TSV file with six columns coming from kraken2 output + pattern: "*.{tsv}" + - database: + type: file + description: Directory containing the kraken2/Bracken files for analysis + pattern: "*" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - reports: + type: file + description: TSV output report of the re-estimated abundances + pattern: "*.{tsv}" + +authors: + - "@Midnighter" From 4f728648f59c35f7282e832090302047c7786f2a Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 14 Oct 2022 10:56:14 +0200 Subject: [PATCH 008/132] feat: add process to standardize kraken report --- modules/local/kraken_standard_report.nf | 24 ++++++++++++++++++++++++ 1 file changed, 24 insertions(+) create mode 100644 modules/local/kraken_standard_report.nf diff --git a/modules/local/kraken_standard_report.nf b/modules/local/kraken_standard_report.nf new file mode 100644 index 0000000..93109aa --- /dev/null +++ b/modules/local/kraken_standard_report.nf @@ -0,0 +1,24 @@ +process KRAKEN_STANDARD_REPORT { + tag "$meta.id" + label 'process_low' + + conda (params.enable_conda ? 'conda-forge::sed=4.8' : null) + if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { + container 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv2/biocontainers_v1.2.0_cv2.img' + } else { + container 'biocontainers/biocontainers:v1.2.0_cv2' + } + + input: + tuple val(meta), path(report) + + output: + tuple val(meta), path(result), emit: report + + script: + result = "${report.baseName}_standardized.kraken2.report.txt" + """ + cut -f1-3,6-8 "${report}" > "${result}" + """ +} + From ca38d002dee9510ca687403a617aec744ab9d46d Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 14 Oct 2022 12:18:07 +0200 Subject: [PATCH 009/132] feat: integrate Bracken into the profiling pipeline --- conf/modules.config | 11 +++++++++- modules/local/kraken_standard_report.nf | 18 +++++++++------- nextflow.config | 4 ++++ subworkflows/local/profiling.nf | 28 +++++++++++++++++++++++++ 4 files changed, 52 insertions(+), 9 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index d2a0051..fff28ea 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -277,7 +277,7 @@ process { } withName: KRAKEN2_KRAKEN2 { - ext.args = { "${meta.db_params}" } + ext.args = params.kraken2_save_minimizers ? { "${meta.db_params} --report-minimizer-data" } : { "${meta.db_params}" } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/kraken2/${meta.db_name}/" }, @@ -286,6 +286,15 @@ process { ] } + withName: BRACKEN_BRACKEN { + errorStrategy = 'ignore' + publishDir = [ + path: { "${params.outdir}/bracken/${meta.db_name}/" }, + mode: params.publish_dir_mode, + pattern: '*.tsv' + ] + } + withName: KRAKENTOOLS_COMBINEKREPORTS { ext.prefix = { "kraken2_${meta.id}_combined_reports" } publishDir = [ diff --git a/modules/local/kraken_standard_report.nf b/modules/local/kraken_standard_report.nf index 93109aa..ab47d81 100644 --- a/modules/local/kraken_standard_report.nf +++ b/modules/local/kraken_standard_report.nf @@ -1,13 +1,11 @@ process KRAKEN_STANDARD_REPORT { tag "$meta.id" - label 'process_low' + label 'process_single' conda (params.enable_conda ? 'conda-forge::sed=4.8' : null) - if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) { - container 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv2/biocontainers_v1.2.0_cv2.img' - } else { - container 'biocontainers/biocontainers:v1.2.0_cv2' - } + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://containers.biocontainers.pro/s3/SingImgsRepo/biocontainers/v1.2.0_cv2/biocontainers_v1.2.0_cv2.img' : + 'biocontainers/biocontainers:v1.2.0_cv2' }" input: tuple val(meta), path(report) @@ -15,10 +13,14 @@ process KRAKEN_STANDARD_REPORT { output: tuple val(meta), path(result), emit: report + when: + task.ext.when == null || task.ext.when + script: - result = "${report.baseName}_standardized.kraken2.report.txt" + def prefix = task.ext.prefix ?: "${meta.id}" + result = "${prefix}_standardized.kraken2.report.txt" """ - cut -f1-3,6-8 "${report}" > "${result}" + cut -f1-3,6-8 '${report}' > '${result}' """ } diff --git a/nextflow.config b/nextflow.config index efb5aff..4f32a00 100644 --- a/nextflow.config +++ b/nextflow.config @@ -114,6 +114,10 @@ params { run_kraken2 = false kraken2_save_reads = false // added directly to module in profiling.nf kraken2_save_readclassification = false // added directly to module in profiling.nf + kraken2_save_minimizers = false + + // Bracken + run_bracken = true // centrifuge run_centrifuge = false diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 11c4a72..970c128 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -5,6 +5,7 @@ include { MALT_RUN } from '../../modules/nf-core/malt/run/main' include { MEGAN_RMA2INFO as MEGAN_RMA2INFO_TSV } from '../../modules/nf-core/megan/rma2info/main' include { KRAKEN2_KRAKEN2 } from '../../modules/nf-core/kraken2/kraken2/main' +include { BRACKEN_BRACKEN } from '../../modules/nf-core/bracken/bracken/main' include { CENTRIFUGE_CENTRIFUGE } from '../../modules/nf-core/centrifuge/centrifuge/main' include { CENTRIFUGE_KREPORT } from '../../modules/nf-core/centrifuge/kreport/main' include { METAPHLAN3_METAPHLAN3 } from '../../modules/nf-core/metaphlan3/metaphlan3/main' @@ -133,6 +134,33 @@ workflow PROFILING { } + if ( params.run_kraken2 && params.run_bracken ) { + + def ch_input_for_bracken + + if (params.kraken2_save_minimizers) { + ch_input_for_bracken = KRAKEN_STANDARD_REPORT(KRAKEN2_KRAKEN2.out.report).report + } else { + ch_input_for_bracken = KRAKEN2_KRAKEN2.out.report + } + + ch_input_for_bracken = ch_input_for_bracken + .combine( + databases.filter { meta, db -> + meta['tool'] == 'bracken' + } + ) + .multiMap { meta, report, db_meta, db -> + report: [meta + db_meta, report] + db: db + } + + BRACKEN_BRACKEN(ch_input_for_bracken.report, ch_input_for_bracken.db) + ch_versions = ch_versions.mix(BRACKEN_BRACKEN.out.versions.first()) + ch_raw_profiles = ch_raw_profiles.mix(BRACKEN_BRACKEN.out.reports) + + } + if ( params.run_centrifuge ) { ch_input_for_centrifuge = ch_input_for_profiling.centrifuge From 767663d975040baed045d7dcc49a290e292b54a8 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 14 Oct 2022 12:18:55 +0200 Subject: [PATCH 010/132] chore: add Bracken to test configs --- conf/test.config | 1 + conf/test_nopreprocessing.config | 1 + conf/test_noprofiling.config | 1 + conf/test_nothing.config | 1 + conf/test_pep.config | 1 + 5 files changed, 5 insertions(+) diff --git a/conf/test.config b/conf/test.config index d5dcd67..777d9bf 100644 --- a/conf/test.config +++ b/conf/test.config @@ -34,6 +34,7 @@ params { hostremoval_reference = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' run_kaiju = true run_kraken2 = true + run_bracken = true run_malt = true run_metaphlan3 = true run_centrifuge = true diff --git a/conf/test_nopreprocessing.config b/conf/test_nopreprocessing.config index 3908b56..357f76f 100644 --- a/conf/test_nopreprocessing.config +++ b/conf/test_nopreprocessing.config @@ -33,6 +33,7 @@ params { hostremoval_reference = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' run_kaiju = true run_kraken2 = true + run_bracken = true run_malt = true run_metaphlan3 = true run_centrifuge = true diff --git a/conf/test_noprofiling.config b/conf/test_noprofiling.config index 12c7185..59ed0da 100644 --- a/conf/test_noprofiling.config +++ b/conf/test_noprofiling.config @@ -34,6 +34,7 @@ params { hostremoval_reference = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' run_kaiju = false run_kraken2 = false + run_bracken = false run_malt = false run_metaphlan3 = false run_centrifuge = false diff --git a/conf/test_nothing.config b/conf/test_nothing.config index c0ecece..df09613 100644 --- a/conf/test_nothing.config +++ b/conf/test_nothing.config @@ -33,6 +33,7 @@ params { hostremoval_reference = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' run_kaiju = false run_kraken2 = false + run_bracken = false run_malt = false run_metaphlan3 = false run_centrifuge = false diff --git a/conf/test_pep.config b/conf/test_pep.config index 7f8c95d..762ebb3 100644 --- a/conf/test_pep.config +++ b/conf/test_pep.config @@ -19,6 +19,7 @@ params { hostremoval_reference = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' run_kaiju = true run_kraken2 = true + run_bracken = true run_malt = true run_metaphlan3 = true run_centrifuge = true From 7f8180f45841cc3ffb40ca70c564a8d14ed254f7 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 14 Oct 2022 12:49:07 +0200 Subject: [PATCH 011/132] fix: emit tool versions --- modules/local/kraken_standard_report.nf | 6 ++++++ 1 file changed, 6 insertions(+) diff --git a/modules/local/kraken_standard_report.nf b/modules/local/kraken_standard_report.nf index ab47d81..aada6fb 100644 --- a/modules/local/kraken_standard_report.nf +++ b/modules/local/kraken_standard_report.nf @@ -12,6 +12,7 @@ process KRAKEN_STANDARD_REPORT { output: tuple val(meta), path(result), emit: report + path 'versions.yml' , emit: versions when: task.ext.when == null || task.ext.when @@ -21,6 +22,11 @@ process KRAKEN_STANDARD_REPORT { result = "${prefix}_standardized.kraken2.report.txt" """ cut -f1-3,6-8 '${report}' > '${result}' + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + cut: \$(echo \$(cut --version 2>&1) | sed 's/^.*(GNU coreutils) //; s/ Copyright.*\$//') + END_VERSIONS """ } From c4f64682751383b6144d14d05cfa48e2d10b6235 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 14 Oct 2022 12:55:26 +0200 Subject: [PATCH 012/132] chore: update parameters schema --- nextflow_schema.json | 61 +++++++++++++++++++++++++++++++++++++++----- 1 file changed, 54 insertions(+), 7 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index f88443f..c40ce0e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,11 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir", "databases"], + "required": [ + "input", + "databases", + "outdir" + ], "properties": { "input": { "type": "string", @@ -80,7 +84,10 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": ["fastp", "adapterremoval"], + "enum": [ + "fastp", + "adapterremoval" + ], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -133,7 +140,11 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], + "enum": [ + "bbduk", + "prinseqplusplus", + "fastp" + ], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -167,7 +178,10 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": ["entropy", "dust"], + "enum": [ + "entropy", + "dust" + ], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -341,7 +355,15 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "enum": [ + "blast", + "xml", + "txt", + "daa", + "sam", + "tsv", + "paf" + ], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -360,7 +382,14 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], + "enum": [ + "phylum", + "class", + "order", + "family", + "genus", + "species" + ], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -382,6 +411,17 @@ "description": "Turn on saving of Kraken2 per-read taxonomic assignment file", "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - kraken2: `--output`" }, + "kraken2_save_minimizers": { + "type": "boolean", + "description": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.", + "fa_icon": "fas fa-save", + "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." + }, + "run_bracken": { + "type": "boolean", + "description": "Post-process kraken2 reports with Bracken.", + "fa_icon": "fas fa-toggle-on" + }, "run_malt": { "type": "boolean", "fa_icon": "fas fa-toggle-on", @@ -555,7 +595,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { From 9dc819ceff9db68b214573062f07a7dad60ef593 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 14 Oct 2022 13:16:54 +0200 Subject: [PATCH 013/132] style: reformat schema --- nextflow_schema.json | 50 +++++++------------------------------------- 1 file changed, 7 insertions(+), 43 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index c40ce0e..97a03b5 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,11 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "databases", - "outdir" - ], + "required": ["input", "databases", "outdir"], "properties": { "input": { "type": "string", @@ -84,10 +80,7 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], + "enum": ["fastp", "adapterremoval"], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -140,11 +133,7 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], + "enum": ["bbduk", "prinseqplusplus", "fastp"], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -178,10 +167,7 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": [ - "entropy", - "dust" - ], + "enum": ["entropy", "dust"], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -355,15 +341,7 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -382,14 +360,7 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], + "enum": ["phylum", "class", "order", "family", "genus", "species"], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -595,14 +566,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { From 4f2799885207b278c2795c8a4011a750ae4a8606 Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Fri, 14 Oct 2022 14:37:52 +0200 Subject: [PATCH 014/132] Update usage.md --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 4f4c1ac..9194220 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -230,7 +230,7 @@ You can optionally save the FASTQ output of the run merging with the `--save_run ###### MALT -nf-core/taxprofiler uses MALT 0.4.1, which is a compatively old version. However it has been found that the most recent version of MALT (0.5.\*), at the time of writing, is broken. [The the LCA step appears not to be executed](http://megan.informatik.uni-tuebingen.de/t/lca-placement-failure-with-malt-v-0-5-2-and-0-5-3/1996/3), pushing all hits to the leaves of the taxonomy. However, if you need to use a more recent taxonomy map file with your databases, the output of `malt-build` from MALT 0.5.3 should be still be compatible with `malt-run` of 0.4.1. +nf-core/taxprofiler uses MALT 0.4.1, which is a compatively old version. However it has been found that the most recent version of MALT (0.5.\*), at the time of writing, is broken. [The LCA step appears not to be executed](http://megan.informatik.uni-tuebingen.de/t/lca-placement-failure-with-malt-v-0-5-2-and-0-5-3/1996/3), pushing all hits to the leaves of the taxonomy. However, if you need to use a more recent taxonomy map file with your databases, the output of `malt-build` from MALT 0.5.3 should be still be compatible with `malt-run` of 0.4.1. ### Updating the pipeline From 95621ebd8ad4b89e2cdad0594f64125d07ac3f66 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 14 Oct 2022 14:47:08 +0200 Subject: [PATCH 015/132] fix: include local module --- subworkflows/local/profiling.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 970c128..4d49ce2 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -5,6 +5,7 @@ include { MALT_RUN } from '../../modules/nf-core/malt/run/main' include { MEGAN_RMA2INFO as MEGAN_RMA2INFO_TSV } from '../../modules/nf-core/megan/rma2info/main' include { KRAKEN2_KRAKEN2 } from '../../modules/nf-core/kraken2/kraken2/main' +include { KRAKEN_STANDARD_REPORT } from '../../modules/local/kraken_standard_report' include { BRACKEN_BRACKEN } from '../../modules/nf-core/bracken/bracken/main' include { CENTRIFUGE_CENTRIFUGE } from '../../modules/nf-core/centrifuge/centrifuge/main' include { CENTRIFUGE_KREPORT } from '../../modules/nf-core/centrifuge/kreport/main' From 563b34c9c38535a11a3c14d488fcd97519972d81 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 14 Oct 2022 14:48:27 +0200 Subject: [PATCH 016/132] refactor: rename process to kraken2 --- .../{kraken_standard_report.nf => kraken2_standard_report.nf} | 2 +- subworkflows/local/profiling.nf | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) rename modules/local/{kraken_standard_report.nf => kraken2_standard_report.nf} (96%) diff --git a/modules/local/kraken_standard_report.nf b/modules/local/kraken2_standard_report.nf similarity index 96% rename from modules/local/kraken_standard_report.nf rename to modules/local/kraken2_standard_report.nf index aada6fb..09a98c1 100644 --- a/modules/local/kraken_standard_report.nf +++ b/modules/local/kraken2_standard_report.nf @@ -1,4 +1,4 @@ -process KRAKEN_STANDARD_REPORT { +process KRAKEN2_STANDARD_REPORT { tag "$meta.id" label 'process_single' diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 4d49ce2..0d46e0c 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -5,7 +5,7 @@ include { MALT_RUN } from '../../modules/nf-core/malt/run/main' include { MEGAN_RMA2INFO as MEGAN_RMA2INFO_TSV } from '../../modules/nf-core/megan/rma2info/main' include { KRAKEN2_KRAKEN2 } from '../../modules/nf-core/kraken2/kraken2/main' -include { KRAKEN_STANDARD_REPORT } from '../../modules/local/kraken_standard_report' +include { KRAKEN2_STANDARD_REPORT } from '../../modules/local/kraken2_standard_report' include { BRACKEN_BRACKEN } from '../../modules/nf-core/bracken/bracken/main' include { CENTRIFUGE_CENTRIFUGE } from '../../modules/nf-core/centrifuge/centrifuge/main' include { CENTRIFUGE_KREPORT } from '../../modules/nf-core/centrifuge/kreport/main' @@ -140,7 +140,7 @@ workflow PROFILING { def ch_input_for_bracken if (params.kraken2_save_minimizers) { - ch_input_for_bracken = KRAKEN_STANDARD_REPORT(KRAKEN2_KRAKEN2.out.report).report + ch_input_for_bracken = KRAKEN2_STANDARD_REPORT(KRAKEN2_KRAKEN2.out.report).report } else { ch_input_for_bracken = KRAKEN2_KRAKEN2.out.report } From fd8a0df2a68fb17a0422b281458d1267b824d004 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Mon, 17 Oct 2022 17:16:21 +0200 Subject: [PATCH 017/132] refactor: add warning when run_kraken2 is missing --- workflows/taxprofiler.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/workflows/taxprofiler.nf b/workflows/taxprofiler.nf index 8b9edb7..f6dc3ed 100644 --- a/workflows/taxprofiler.nf +++ b/workflows/taxprofiler.nf @@ -41,6 +41,7 @@ if (params.longread_hostremoval_index ) { ch_longread_reference_index = fi if (params.diamond_save_reads ) log.warn "[nf-core/taxprofiler] DIAMOND only allows output of a single format. As --diamond_save_reads supplied, only aligned reads in SAM format will be produced, no taxonomic profiles will be available." if (params.run_malt && params.run_krona && !params.krona_taxonomy_directory) log.warn "[nf-core/taxprofiler] Krona can only be run on MALT output if path to Krona taxonomy database supplied to --krona_taxonomy_directory. Krona will not be executed in this run for MALT." +if (params.run_bracken && !params.run_kraken2) log.warn '[nf-core/taxprofiler] You are attempting to run Bracken without running kraken2. This is not possible! Please set --run_kraken2 as well.' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From 48b00b7d624d9d37a4b891a578ea23e805bf6543 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Mon, 17 Oct 2022 17:33:24 +0200 Subject: [PATCH 018/132] refactor: create standardized Bracken reports --- .../local/standardisation_profiles.nf | 25 +++++++++++++++++-- 1 file changed, 23 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/standardisation_profiles.nf b/subworkflows/local/standardisation_profiles.nf index cbb0fab..f560424 100644 --- a/subworkflows/local/standardisation_profiles.nf +++ b/subworkflows/local/standardisation_profiles.nf @@ -3,8 +3,11 @@ // include { KAIJU_KAIJU2TABLE } from '../../modules/nf-core/kaiju/kaiju2table/main' -include { KRAKENTOOLS_COMBINEKREPORTS } from '../../modules/nf-core/krakentools/combinekreports/main' -include { KRAKENTOOLS_COMBINEKREPORTS as KRAKENTOOLS_COMBINEKREPORTS_CENTRIFUGE } from '../../modules/nf-core/krakentools/combinekreports/main' +include { + KRAKENTOOLS_COMBINEKREPORTS as KRAKENTOOLS_COMBINEKREPORTS_CENTRIFUGE; + KRAKENTOOLS_COMBINEKREPORTS as KRAKENTOOLS_COMBINEKREPORTS_BRACKEN; + KRAKENTOOLS_COMBINEKREPORTS +} from '../../modules/nf-core/krakentools/combinekreports/main' include { METAPHLAN3_MERGEMETAPHLANTABLES } from '../../modules/nf-core/metaphlan3/mergemetaphlantables/main' include { MOTUS_MERGE } from '../../modules/nf-core/motus/merge/main' @@ -27,6 +30,7 @@ workflow STANDARDISATION_PROFILES { .branch { motus: it[0]['tool'] == 'motus' kraken2: it[0]['tool'] == 'kraken2' + bracken: it[0]['tool'] == 'bracken' centrifuge: it[0]['tool'] == 'centrifuge' metaphlan3: it[0]['tool'] == 'metaphlan3' unknown: true @@ -98,6 +102,23 @@ workflow STANDARDISATION_PROFILES { ch_multiqc_files = ch_multiqc_files.mix( KRAKENTOOLS_COMBINEKREPORTS.out.txt ) ch_versions = ch_versions.mix( KRAKENTOOLS_COMBINEKREPORTS.out.versions ) + // Bracken + + // Collect and replace id for db_name for prefix + // Have to sort by size to ensure first file actually has hits otherwise + // the script fails + ch_profiles_for_bracken = ch_input_profiles.bracken + .map { [it[0]['db_name'], it[1]] } + .groupTuple(sort: {-it.size()} ) + .map { + [[id:it[0]], it[1]] + } + + KRAKENTOOLS_COMBINEKREPORTS_BRACKEN ( ch_profiles_for_bracken ) + ch_standardised_tables = ch_standardised_tables.mix( KRAKENTOOLS_COMBINEKREPORTS_BRACKEN.out.txt ) + ch_multiqc_files = ch_multiqc_files.mix( KRAKENTOOLS_COMBINEKREPORTS_BRACKEN.out.txt ) + ch_versions = ch_versions.mix( KRAKENTOOLS_COMBINEKREPORTS_BRACKEN.out.versions ) + // MetaPhlAn3 ch_profiles_for_metaphlan3 = ch_input_profiles.metaphlan3 From 8768a16d63231e07daf3d8cf8fbf1afd930b2a1c Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Mon, 17 Oct 2022 17:35:41 +0200 Subject: [PATCH 019/132] refactor: turn warning into error --- workflows/taxprofiler.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/taxprofiler.nf b/workflows/taxprofiler.nf index f6dc3ed..12ee19f 100644 --- a/workflows/taxprofiler.nf +++ b/workflows/taxprofiler.nf @@ -41,7 +41,7 @@ if (params.longread_hostremoval_index ) { ch_longread_reference_index = fi if (params.diamond_save_reads ) log.warn "[nf-core/taxprofiler] DIAMOND only allows output of a single format. As --diamond_save_reads supplied, only aligned reads in SAM format will be produced, no taxonomic profiles will be available." if (params.run_malt && params.run_krona && !params.krona_taxonomy_directory) log.warn "[nf-core/taxprofiler] Krona can only be run on MALT output if path to Krona taxonomy database supplied to --krona_taxonomy_directory. Krona will not be executed in this run for MALT." -if (params.run_bracken && !params.run_kraken2) log.warn '[nf-core/taxprofiler] You are attempting to run Bracken without running kraken2. This is not possible! Please set --run_kraken2 as well.' +if (params.run_bracken && !params.run_kraken2) exit 1, 'ERROR: [nf-core/taxprofiler] You are attempting to run Bracken without running kraken2. This is not possible! Please set --run_kraken2 as well.' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From 8eddb32b8807db582ca7fd1e31074c50788162ba Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Tue, 18 Oct 2022 17:43:16 +0200 Subject: [PATCH 020/132] Add Falco as an alternative to FastQC --- .github/workflows/ci.yml | 1 + CITATIONS.md | 4 ++ README.md | 2 +- conf/modules.config | 18 ++++++ docs/usage.md | 4 +- modules.json | 4 ++ modules/nf-core/falco/main.nf | 57 +++++++++++++++++++ modules/nf-core/falco/meta.yml | 52 +++++++++++++++++ nextflow_schema.json | 50 +++++++++++++--- subworkflows/local/longread_preprocessing.nf | 12 +++- subworkflows/local/shortread_preprocessing.nf | 16 ++++-- workflows/taxprofiler.nf | 19 ++++--- 12 files changed, 217 insertions(+), 22 deletions(-) create mode 100644 modules/nf-core/falco/main.nf create mode 100644 modules/nf-core/falco/meta.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 5cea5b7..344505a 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -23,6 +23,7 @@ jobs: - "21.10.3" - "latest-everything" parameters: + - "--perform_fastqc_alternative false" - "--perform_longread_qc false" - "--perform_shortread_qc false" - "--shortread_qc_tool fastp" diff --git a/CITATIONS.md b/CITATIONS.md index 1ce4ec2..510c74b 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -62,6 +62,10 @@ - [FILTLONG](https://github.com/rrwick/Filtlong) +- [Falco](https://doi.org/10.12688/f1000research.21142.2) + +> de Sena Brandine G and Smith AD. Falco: high-speed FastQC emulation for quality control of sequencing data. F1000Research 2021, 8:1874 + ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) diff --git a/README.md b/README.md index 2bd45a9..e17c7b5 100644 --- a/README.md +++ b/README.md @@ -30,7 +30,7 @@ On release, automated continuous integration tests run the pipeline on a full-si ![](docs/images/taxprofiler_tube.png) -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) or [`Falco`](https://github.com/smithlabcode/falco) as an alternative option) 2. Performs optional read pre-processing - Adapter clipping and merging (short-read: [fastp](https://github.com/OpenGene/fastp), [AdapterRemoval2](https://github.com/MikkelSchubert/adapterremoval); long-read: [porechop](https://github.com/rrwick/Porechop)) - Low complexity and quality filtering (short-read: [bbduk](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/), [PRINSEQ++](https://github.com/Adrian-Cantu/PRINSEQ-plus-plus); long-read: [Filtlong](https://github.com/rrwick/Filtlong)) diff --git a/conf/modules.config b/conf/modules.config index d2a0051..1e2058a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -40,6 +40,24 @@ process { ] } + withName: FALCO { + ext.prefix = { "${meta.id}_${meta.run_accession}_raw" } + publishDir = [ + path: { "${params.outdir}/falco/raw" }, + mode: params.publish_dir_mode, + pattern: '*.{html,txt}' + ] + } + + withName: FALCO_PROCESSED { + ext.prefix = { "${meta.id}_${meta.run_accession}_processed" } + publishDir = [ + path: { "${params.outdir}/falco/processed" }, + mode: params.publish_dir_mode, + pattern: '*.{html,txt}' + ] + } + withName: FASTP_SINGLE { ext.args = [ // trimming options diff --git a/docs/usage.md b/docs/usage.md index 9194220..7478636 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -165,7 +165,9 @@ work # Directory containing the nextflow working files .nextflow_log # Log file from Nextflow # Other nextflow hidden files, eg. history of pipeline runs and old logs. ``` +### Sequencing quality control +nf-core taxprofiler offers [`Falco`](https://github.com/smithlabcode/falco] as an alternative option to [`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/). ### Preprocessing Steps nf-core/taxprofiler offers four main preprocessing steps @@ -179,7 +181,7 @@ nf-core/taxprofiler offers four main preprocessing steps Raw sequencing read processing in the form of adapter clipping and paired-end read merging can be activated via the `--perform_shortread_qc` or `--perform_longread_qc` flags. -It is highly recommended to run this on raw reads to remove artefacts from sequencing that can cause false positive identification of taxa (e.g. contaminated reference genomes) and/or skews in taxonomic abundance profiles. +It is highly recommended to run this on raw reads to remove artifacts from sequencing that can cause false positive identification of taxa (e.g. contaminated reference genomes) and/or skews in taxonomic abundance profiles. There are currently two options for short-read preprocessing: `fastp` or `adapterremoval`. diff --git a/modules.json b/modules.json index 10d6c74..aa77c4e 100644 --- a/modules.json +++ b/modules.json @@ -49,6 +49,10 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" }, + "falco": { + "branch": "master", + "git_sha": "fc959214036403ad83efe7a41d43d0606c445cda" + }, "fastp": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" diff --git a/modules/nf-core/falco/main.nf b/modules/nf-core/falco/main.nf new file mode 100644 index 0000000..1688162 --- /dev/null +++ b/modules/nf-core/falco/main.nf @@ -0,0 +1,57 @@ +process FALCO { + tag "$meta.id" + label 'process_single' + + + conda (params.enable_conda ? "bioconda::falco=1.2.1" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/falco:1.2.1--h867801b_3': + 'quay.io/biocontainers/falco:1.2.1--h867801b_3' }" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.html"), emit: html + tuple val(meta), path("*.txt") , emit: txt + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + if ( reads.toList().size() == 1 ) { + """ + falco $args --threads $task.cpus ${reads} -D ${prefix}_data.txt -S ${prefix}_summary.txt -R ${prefix}_report.html + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + falco:\$( falco --version | sed -e "s/falco//g" ) + END_VERSIONS + """ + } else { + """ + falco $args --threads $task.cpus ${reads} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + falco:\$( falco --version | sed -e "s/falco//g" ) + END_VERSIONS + """ + } + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}_data.txt + touch ${prefix}_fastqc_data.html + touch ${prefix}_summary.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + falco: \$( falco --version | sed -e "s/falco v//g" ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/falco/meta.yml b/modules/nf-core/falco/meta.yml new file mode 100644 index 0000000..6f77fb1 --- /dev/null +++ b/modules/nf-core/falco/meta.yml @@ -0,0 +1,52 @@ +name: falco +description: Run falco on sequenced reads +keywords: + - quality control + - qc + - adapters + - fastq +tools: + - fastqc: + description: "falco is a drop-in C++ implementation of FastQC to assess the quality of sequence reads." + + homepage: "https://falco.readthedocs.io/" + documentation: "https://falco.readthedocs.io/" + tool_dev_url: "None" + doi: "" + licence: "['GPL v3']" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - html: + type: file + description: FastQC like report + pattern: "*_{fastqc_report.html}" + - txt: + type: file + description: falco report data + pattern: "*_{data.txt}" + - txt: + type: file + description: falco summary file + pattern: "*_{summary.txt}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@lucacozzuto" diff --git a/nextflow_schema.json b/nextflow_schema.json index f88443f..f48ed4a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,11 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir", "databases"], + "required": [ + "input", + "outdir", + "databases" + ], "properties": { "input": { "type": "string", @@ -80,7 +84,10 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": ["fastp", "adapterremoval"], + "enum": [ + "fastp", + "adapterremoval" + ], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -133,7 +140,11 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], + "enum": [ + "bbduk", + "prinseqplusplus", + "fastp" + ], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -167,7 +178,10 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": ["entropy", "dust"], + "enum": [ + "entropy", + "dust" + ], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -341,7 +355,15 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "enum": [ + "blast", + "xml", + "txt", + "daa", + "sam", + "tsv", + "paf" + ], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -360,7 +382,14 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], + "enum": [ + "phylum", + "class", + "order", + "family", + "genus", + "species" + ], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -555,7 +584,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { diff --git a/subworkflows/local/longread_preprocessing.nf b/subworkflows/local/longread_preprocessing.nf index ce537e8..cf0d168 100644 --- a/subworkflows/local/longread_preprocessing.nf +++ b/subworkflows/local/longread_preprocessing.nf @@ -3,6 +3,8 @@ // include { FASTQC as FASTQC_PROCESSED } from '../../modules/nf-core/fastqc/main' +include { FALCO as FALCO_PROCESSED } from '../../modules/nf-core/falco/main' + include { PORECHOP } from '../../modules/nf-core/porechop/main' include { FILTLONG } from '../../modules/nf-core/filtlong/main' @@ -52,8 +54,14 @@ workflow LONGREAD_PREPROCESSING { ch_multiqc_files = ch_multiqc_files.mix( FILTLONG.out.log ) } - FASTQC_PROCESSED ( ch_processed_reads ) - ch_multiqc_files = ch_multiqc_files.mix( FASTQC_PROCESSED.out.zip ) + if (params.perform_fastqc_alternative) { + FALCO_PROCESSED ( ch_processed_reads ) + ch_multiqc_files = ch_multiqc_files.mix( FALCO_PROCESSED.out.txt ) + + } else { + FASTQC_PROCESSED ( ch_processed_reads ) + ch_multiqc_files = ch_multiqc_files.mix( FASTQC_PROCESSED.out.zip ) + } emit: reads = ch_processed_reads // channel: [ val(meta), [ reads ] ] diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index 859c1d5..a67539a 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -5,7 +5,8 @@ include { SHORTREAD_FASTP } from './shortread_fastp' include { SHORTREAD_ADAPTERREMOVAL } from './shortread_adapterremoval' -include { FASTQC as FASTQC_PROCESSED } from '../../modules/nf-core/fastqc/main' +include { FASTQC as FASTQC_PROCESSED } from '../../modules/nf-core/fastqc/main' +include { FALCO as FALCO_PROCESSED } from '../../modules/nf-core/falco/main' workflow SHORTREAD_PREPROCESSING { take: @@ -27,9 +28,16 @@ workflow SHORTREAD_PREPROCESSING { ch_processed_reads = reads } - FASTQC_PROCESSED ( ch_processed_reads ) - ch_versions = ch_versions.mix( FASTQC_PROCESSED.out.versions ) - ch_multiqc_files = ch_multiqc_files.mix( FASTQC_PROCESSED.out.zip ) + if (params.perform_fastqc_alternative) { + FALCO_PROCESSED ( ch_processed_reads ) + ch_versions = ch_versions.mix( FALCO_PROCESSED.out.versions ) + ch_multiqc_files = ch_multiqc_files.mix( FALCO_PROCESSED.out.txt ) + + } else { + FASTQC_PROCESSED ( ch_processed_reads ) + ch_versions = ch_versions.mix( FASTQC_PROCESSED.out.versions ) + ch_multiqc_files = ch_multiqc_files.mix( FASTQC_PROCESSED.out.zip ) + } emit: reads = ch_processed_reads // channel: [ val(meta), [ reads ] ] diff --git a/workflows/taxprofiler.nf b/workflows/taxprofiler.nf index 8b9edb7..2435782 100644 --- a/workflows/taxprofiler.nf +++ b/workflows/taxprofiler.nf @@ -84,6 +84,7 @@ include { STANDARDISATION_PROFILES } from '../subworkflows/local/standardis // MODULE: Installed directly from nf-core/modules // include { FASTQC } from '../modules/nf-core/fastqc/main' +include { FALCO } from '../modules/nf-core/falco/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { CAT_FASTQ } from '../modules/nf-core/cat/fastq/main' @@ -120,12 +121,13 @@ workflow TAXPROFILER { */ ch_input_for_fastqc = INPUT_CHECK.out.fastq.mix( INPUT_CHECK.out.nanopore ) - FASTQC ( - ch_input_for_fastqc - ) - - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - + if ( params.perform_fastqc_alternative ) { + FALCO ( ch_input_for_fastqc ) + ch_versions = ch_versions.mix(FALCO.out.versions.first()) + } else { + FASTQC ( ch_input_for_fastqc ) + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + } /* SUBWORKFLOW: PERFORM PREPROCESSING */ @@ -254,7 +256,10 @@ workflow TAXPROFILER { ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + if (!params.perform_fastqc_alternative) { + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + } if (params.perform_shortread_qc) { ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_PREPROCESSING.out.mqc.collect{it[1]}.ifEmpty([]) ) From 0481bb9449a1d35a8fe8324811180ef68f0d361f Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Tue, 18 Oct 2022 17:48:40 +0200 Subject: [PATCH 021/132] Prettier --- docs/usage.md | 2 ++ nextflow_schema.json | 50 +++++++------------------------------------- 2 files changed, 9 insertions(+), 43 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 7478636..4a44cf4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -165,9 +165,11 @@ work # Directory containing the nextflow working files .nextflow_log # Log file from Nextflow # Other nextflow hidden files, eg. history of pipeline runs and old logs. ``` + ### Sequencing quality control nf-core taxprofiler offers [`Falco`](https://github.com/smithlabcode/falco] as an alternative option to [`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/). + ### Preprocessing Steps nf-core/taxprofiler offers four main preprocessing steps diff --git a/nextflow_schema.json b/nextflow_schema.json index f48ed4a..f88443f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,11 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "outdir", - "databases" - ], + "required": ["input", "outdir", "databases"], "properties": { "input": { "type": "string", @@ -84,10 +80,7 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], + "enum": ["fastp", "adapterremoval"], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -140,11 +133,7 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], + "enum": ["bbduk", "prinseqplusplus", "fastp"], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -178,10 +167,7 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": [ - "entropy", - "dust" - ], + "enum": ["entropy", "dust"], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -355,15 +341,7 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -382,14 +360,7 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], + "enum": ["phylum", "class", "order", "family", "genus", "species"], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -584,14 +555,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { From 7881ba9aefdfd541d49d412edc683728bd82a97a Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Tue, 18 Oct 2022 17:50:49 +0200 Subject: [PATCH 022/132] Fix the amount of left-padding spaces --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 1e2058a..b1d7b87 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -41,7 +41,7 @@ process { } withName: FALCO { - ext.prefix = { "${meta.id}_${meta.run_accession}_raw" } + ext.prefix = { "${meta.id}_${meta.run_accession}_raw" } publishDir = [ path: { "${params.outdir}/falco/raw" }, mode: params.publish_dir_mode, From c7c7a7f6783646a492bb31855d2d47fb36da37f8 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Wed, 19 Oct 2022 16:20:09 +0200 Subject: [PATCH 023/132] refactor: change Bracken prefix to be more specific --- conf/modules.config | 1 + 1 file changed, 1 insertion(+) diff --git a/conf/modules.config b/conf/modules.config index fff28ea..7ec9210 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -288,6 +288,7 @@ process { withName: BRACKEN_BRACKEN { errorStrategy = 'ignore' + ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/bracken/${meta.db_name}/" }, mode: params.publish_dir_mode, From b73b4798600d952575b62a63964b61143b54a438 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Wed, 19 Oct 2022 16:21:36 +0200 Subject: [PATCH 024/132] refactor: handle combined kraken2+bracken tool column --- subworkflows/local/profiling.nf | 25 ++++++++++++++++--------- 1 file changed, 16 insertions(+), 9 deletions(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 0d46e0c..5e2a7d2 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -41,7 +41,7 @@ workflow PROFILING { .combine(databases) .branch { malt: it[2]['tool'] == 'malt' - kraken2: it[2]['tool'] == 'kraken2' + kraken2: it[2]['tool'].contains('kraken2') metaphlan3: it[2]['tool'] == 'metaphlan3' centrifuge: it[2]['tool'] == 'centrifuge' kaiju: it[2]['tool'] == 'kaiju' @@ -131,34 +131,41 @@ workflow PROFILING { ch_multiqc_files = ch_multiqc_files.mix( KRAKEN2_KRAKEN2.out.report ) ch_versions = ch_versions.mix( KRAKEN2_KRAKEN2.out.versions.first() ) ch_raw_classifications = ch_raw_classifications.mix( KRAKEN2_KRAKEN2.out.classified_reads_assignment ) - ch_raw_profiles = ch_raw_profiles.mix( KRAKEN2_KRAKEN2.out.report ) + ch_raw_profiles = ch_raw_profiles.mix( + KRAKEN2_KRAKEN2.out.report + // Set the tool to be strictly 'kraken2' instead of potentially 'kraken2+bracken' for downstream use. + .map { meta, report -> [meta + [tool: 'kraken2'], report]} + ) } if ( params.run_kraken2 && params.run_bracken ) { - def ch_input_for_bracken + def ch_input_for_bracken = KRAKEN2_KRAKEN2.out.report + .filter { meta, report -> meta['tool'].contains('bracken') } if (params.kraken2_save_minimizers) { - ch_input_for_bracken = KRAKEN2_STANDARD_REPORT(KRAKEN2_KRAKEN2.out.report).report - } else { - ch_input_for_bracken = KRAKEN2_KRAKEN2.out.report + ch_input_for_bracken = KRAKEN2_STANDARD_REPORT(ch_input_for_bracken).report } ch_input_for_bracken = ch_input_for_bracken .combine( databases.filter { meta, db -> - meta['tool'] == 'bracken' + meta['tool'].contains('bracken') } ) .multiMap { meta, report, db_meta, db -> - report: [meta + db_meta, report] + report: [meta, report] db: db } BRACKEN_BRACKEN(ch_input_for_bracken.report, ch_input_for_bracken.db) ch_versions = ch_versions.mix(BRACKEN_BRACKEN.out.versions.first()) - ch_raw_profiles = ch_raw_profiles.mix(BRACKEN_BRACKEN.out.reports) + ch_raw_profiles = ch_raw_profiles.mix( + BRACKEN_BRACKEN.out.reports + // Set the tool to be strictly 'bracken' instead of potentially 'kraken2+bracken' for downstream use. + .map { meta, report -> [meta + [tool: 'bracken'], report]} + ) } From 8deb7757d8a260fb2bc4c5890a10995181d1bafe Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Wed, 19 Oct 2022 16:21:57 +0200 Subject: [PATCH 025/132] fix: remove Bracken from standardise reports --- .../local/standardisation_profiles.nf | 25 ++----------------- 1 file changed, 2 insertions(+), 23 deletions(-) diff --git a/subworkflows/local/standardisation_profiles.nf b/subworkflows/local/standardisation_profiles.nf index f560424..cbb0fab 100644 --- a/subworkflows/local/standardisation_profiles.nf +++ b/subworkflows/local/standardisation_profiles.nf @@ -3,11 +3,8 @@ // include { KAIJU_KAIJU2TABLE } from '../../modules/nf-core/kaiju/kaiju2table/main' -include { - KRAKENTOOLS_COMBINEKREPORTS as KRAKENTOOLS_COMBINEKREPORTS_CENTRIFUGE; - KRAKENTOOLS_COMBINEKREPORTS as KRAKENTOOLS_COMBINEKREPORTS_BRACKEN; - KRAKENTOOLS_COMBINEKREPORTS -} from '../../modules/nf-core/krakentools/combinekreports/main' +include { KRAKENTOOLS_COMBINEKREPORTS } from '../../modules/nf-core/krakentools/combinekreports/main' +include { KRAKENTOOLS_COMBINEKREPORTS as KRAKENTOOLS_COMBINEKREPORTS_CENTRIFUGE } from '../../modules/nf-core/krakentools/combinekreports/main' include { METAPHLAN3_MERGEMETAPHLANTABLES } from '../../modules/nf-core/metaphlan3/mergemetaphlantables/main' include { MOTUS_MERGE } from '../../modules/nf-core/motus/merge/main' @@ -30,7 +27,6 @@ workflow STANDARDISATION_PROFILES { .branch { motus: it[0]['tool'] == 'motus' kraken2: it[0]['tool'] == 'kraken2' - bracken: it[0]['tool'] == 'bracken' centrifuge: it[0]['tool'] == 'centrifuge' metaphlan3: it[0]['tool'] == 'metaphlan3' unknown: true @@ -102,23 +98,6 @@ workflow STANDARDISATION_PROFILES { ch_multiqc_files = ch_multiqc_files.mix( KRAKENTOOLS_COMBINEKREPORTS.out.txt ) ch_versions = ch_versions.mix( KRAKENTOOLS_COMBINEKREPORTS.out.versions ) - // Bracken - - // Collect and replace id for db_name for prefix - // Have to sort by size to ensure first file actually has hits otherwise - // the script fails - ch_profiles_for_bracken = ch_input_profiles.bracken - .map { [it[0]['db_name'], it[1]] } - .groupTuple(sort: {-it.size()} ) - .map { - [[id:it[0]], it[1]] - } - - KRAKENTOOLS_COMBINEKREPORTS_BRACKEN ( ch_profiles_for_bracken ) - ch_standardised_tables = ch_standardised_tables.mix( KRAKENTOOLS_COMBINEKREPORTS_BRACKEN.out.txt ) - ch_multiqc_files = ch_multiqc_files.mix( KRAKENTOOLS_COMBINEKREPORTS_BRACKEN.out.txt ) - ch_versions = ch_versions.mix( KRAKENTOOLS_COMBINEKREPORTS_BRACKEN.out.versions ) - // MetaPhlAn3 ch_profiles_for_metaphlan3 = ch_input_profiles.metaphlan3 From 511cb16a6119fe746950abc5fed4100517a41775 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Thu, 20 Oct 2022 16:55:57 +0200 Subject: [PATCH 026/132] Apply review suggestions --- .github/workflows/ci.yml | 3 +- CITATIONS.md | 2 +- README.md | 2 +- nextflow.config | 2 + nextflow_schema.json | 62 ++++++++++++++++--- subworkflows/local/longread_preprocessing.nf | 10 +-- subworkflows/local/shortread_preprocessing.nf | 11 ++-- workflows/taxprofiler.nf | 7 ++- 8 files changed, 75 insertions(+), 24 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 344505a..12185fe 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -23,7 +23,8 @@ jobs: - "21.10.3" - "latest-everything" parameters: - - "--perform_fastqc_alternative false" + - "--preprocessing_qc_tool fastqc" + - "--preprocessing_qc_tool falco" - "--perform_longread_qc false" - "--perform_shortread_qc false" - "--shortread_qc_tool fastp" diff --git a/CITATIONS.md b/CITATIONS.md index 510c74b..599e049 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -62,7 +62,7 @@ - [FILTLONG](https://github.com/rrwick/Filtlong) -- [Falco](https://doi.org/10.12688/f1000research.21142.2) +- [falco](https://doi.org/10.12688/f1000research.21142.2) > de Sena Brandine G and Smith AD. Falco: high-speed FastQC emulation for quality control of sequencing data. F1000Research 2021, 8:1874 diff --git a/README.md b/README.md index e17c7b5..3f0a840 100644 --- a/README.md +++ b/README.md @@ -30,7 +30,7 @@ On release, automated continuous integration tests run the pipeline on a full-si ![](docs/images/taxprofiler_tube.png) -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) or [`Falco`](https://github.com/smithlabcode/falco) as an alternative option) +1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) or [`falco`](https://github.com/smithlabcode/falco) as an alternative option) 2. Performs optional read pre-processing - Adapter clipping and merging (short-read: [fastp](https://github.com/OpenGene/fastp), [AdapterRemoval2](https://github.com/MikkelSchubert/adapterremoval); long-read: [porechop](https://github.com/rrwick/Porechop)) - Low complexity and quality filtering (short-read: [bbduk](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/), [PRINSEQ++](https://github.com/Adrian-Cantu/PRINSEQ-plus-plus); long-read: [Filtlong](https://github.com/rrwick/Filtlong)) diff --git a/nextflow.config b/nextflow.config index efb5aff..b69948e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -59,6 +59,8 @@ params { // Databases databases = null + preprocessing_qc_tool = 'fastqc' + // FASTQ preprocessing perform_shortread_qc = false shortread_qc_tool = 'fastp' diff --git a/nextflow_schema.json b/nextflow_schema.json index f88443f..055c368 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,11 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir", "databases"], + "required": [ + "input", + "outdir", + "databases" + ], "properties": { "input": { "type": "string", @@ -80,7 +84,10 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": ["fastp", "adapterremoval"], + "enum": [ + "fastp", + "adapterremoval" + ], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -133,7 +140,11 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], + "enum": [ + "bbduk", + "prinseqplusplus", + "fastp" + ], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -167,7 +178,10 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": ["entropy", "dust"], + "enum": [ + "entropy", + "dust" + ], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -341,7 +355,15 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "enum": [ + "blast", + "xml", + "txt", + "daa", + "sam", + "tsv", + "paf" + ], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -360,7 +382,14 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], + "enum": [ + "phylum", + "class", + "order", + "family", + "genus", + "species" + ], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -555,7 +584,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { @@ -707,5 +743,15 @@ { "$ref": "#/definitions/reference_genome_options" } - ] + ], + "properties": { + "preprocessing_qc_tool": { + "type": "string", + "default": "fastqc", + "enum": [ + "fastqc", + "falco" + ] + } + } } diff --git a/subworkflows/local/longread_preprocessing.nf b/subworkflows/local/longread_preprocessing.nf index cf0d168..d16db3f 100644 --- a/subworkflows/local/longread_preprocessing.nf +++ b/subworkflows/local/longread_preprocessing.nf @@ -54,13 +54,13 @@ workflow LONGREAD_PREPROCESSING { ch_multiqc_files = ch_multiqc_files.mix( FILTLONG.out.log ) } - if (params.perform_fastqc_alternative) { - FALCO_PROCESSED ( ch_processed_reads ) - ch_multiqc_files = ch_multiqc_files.mix( FALCO_PROCESSED.out.txt ) - - } else { + if (params.preprocessing_qc_tool == 'fastqc') { FASTQC_PROCESSED ( ch_processed_reads ) ch_multiqc_files = ch_multiqc_files.mix( FASTQC_PROCESSED.out.zip ) + + } else if (params.preprocessing_qc_tool == 'falco') { + FALCO_PROCESSED ( ch_processed_reads ) + ch_multiqc_files = ch_multiqc_files.mix( FALCO_PROCESSED.out.txt ) } emit: diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index a67539a..f2cd738 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -28,15 +28,14 @@ workflow SHORTREAD_PREPROCESSING { ch_processed_reads = reads } - if (params.perform_fastqc_alternative) { - FALCO_PROCESSED ( ch_processed_reads ) - ch_versions = ch_versions.mix( FALCO_PROCESSED.out.versions ) - ch_multiqc_files = ch_multiqc_files.mix( FALCO_PROCESSED.out.txt ) - - } else { + if (params.preprocessing_qc_tool == 'fastqc') { FASTQC_PROCESSED ( ch_processed_reads ) ch_versions = ch_versions.mix( FASTQC_PROCESSED.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( FASTQC_PROCESSED.out.zip ) + } else if (params.preprocessing_qc_tool == 'falco') { + FALCO_PROCESSED ( ch_processed_reads ) + ch_versions = ch_versions.mix( FALCO_PROCESSED.out.versions ) + ch_multiqc_files = ch_multiqc_files.mix( FALCO_PROCESSED.out.txt ) } emit: diff --git a/workflows/taxprofiler.nf b/workflows/taxprofiler.nf index 2435782..905cbd9 100644 --- a/workflows/taxprofiler.nf +++ b/workflows/taxprofiler.nf @@ -121,7 +121,7 @@ workflow TAXPROFILER { */ ch_input_for_fastqc = INPUT_CHECK.out.fastq.mix( INPUT_CHECK.out.nanopore ) - if ( params.perform_fastqc_alternative ) { + if ( params.preprocessing_qc_tool == 'falco' ) { FALCO ( ch_input_for_fastqc ) ch_versions = ch_versions.mix(FALCO.out.versions.first()) } else { @@ -257,9 +257,12 @@ workflow TAXPROFILER { ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - if (!params.perform_fastqc_alternative) { + if (!params.preprocessing_qc_tool == 'falco') { ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) } + else { + ch_multiqc_files = ch_multiqc_files.mix(FALCO.out.txt.collect{it[1]}.ifEmpty([])) + } if (params.perform_shortread_qc) { ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_PREPROCESSING.out.mqc.collect{it[1]}.ifEmpty([]) ) From 19231538daac4365596f9e827680fb007df53079 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Thu, 20 Oct 2022 16:59:32 +0200 Subject: [PATCH 027/132] Prettier --- nextflow_schema.json | 55 +++++++------------------------------------- 1 file changed, 8 insertions(+), 47 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 055c368..ff2505f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,11 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "outdir", - "databases" - ], + "required": ["input", "outdir", "databases"], "properties": { "input": { "type": "string", @@ -84,10 +80,7 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], + "enum": ["fastp", "adapterremoval"], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -140,11 +133,7 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], + "enum": ["bbduk", "prinseqplusplus", "fastp"], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -178,10 +167,7 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": [ - "entropy", - "dust" - ], + "enum": ["entropy", "dust"], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -355,15 +341,7 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -382,14 +360,7 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], + "enum": ["phylum", "class", "order", "family", "genus", "species"], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -584,14 +555,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { @@ -748,10 +712,7 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": [ - "fastqc", - "falco" - ] + "enum": ["fastqc", "falco"] } } } From 385507ec6fbb84fa2c3e09ba90365abdf98aac18 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 21 Oct 2022 10:03:36 +0200 Subject: [PATCH 028/132] refactor: use tool name 'bracken' only --- subworkflows/local/profiling.nf | 16 ++++++---------- 1 file changed, 6 insertions(+), 10 deletions(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 5e2a7d2..e76db6c 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -41,7 +41,7 @@ workflow PROFILING { .combine(databases) .branch { malt: it[2]['tool'] == 'malt' - kraken2: it[2]['tool'].contains('kraken2') + kraken2: it[2]['tool'] == 'kraken2' || it[2]['tool'] == 'bracken' metaphlan3: it[2]['tool'] == 'metaphlan3' centrifuge: it[2]['tool'] == 'centrifuge' kaiju: it[2]['tool'] == 'kaiju' @@ -133,7 +133,7 @@ workflow PROFILING { ch_raw_classifications = ch_raw_classifications.mix( KRAKEN2_KRAKEN2.out.classified_reads_assignment ) ch_raw_profiles = ch_raw_profiles.mix( KRAKEN2_KRAKEN2.out.report - // Set the tool to be strictly 'kraken2' instead of potentially 'kraken2+bracken' for downstream use. + // Set the tool to be strictly 'kraken2' instead of potentially 'bracken' for downstream use. .map { meta, report -> [meta + [tool: 'kraken2'], report]} ) @@ -142,7 +142,7 @@ workflow PROFILING { if ( params.run_kraken2 && params.run_bracken ) { def ch_input_for_bracken = KRAKEN2_KRAKEN2.out.report - .filter { meta, report -> meta['tool'].contains('bracken') } + .filter { meta, report -> meta['tool'] == 'bracken' } if (params.kraken2_save_minimizers) { ch_input_for_bracken = KRAKEN2_STANDARD_REPORT(ch_input_for_bracken).report @@ -151,21 +151,17 @@ workflow PROFILING { ch_input_for_bracken = ch_input_for_bracken .combine( databases.filter { meta, db -> - meta['tool'].contains('bracken') + meta['tool'] == 'bracken' } ) .multiMap { meta, report, db_meta, db -> - report: [meta, report] + report: [meta + db_meta, report] db: db } BRACKEN_BRACKEN(ch_input_for_bracken.report, ch_input_for_bracken.db) ch_versions = ch_versions.mix(BRACKEN_BRACKEN.out.versions.first()) - ch_raw_profiles = ch_raw_profiles.mix( - BRACKEN_BRACKEN.out.reports - // Set the tool to be strictly 'bracken' instead of potentially 'kraken2+bracken' for downstream use. - .map { meta, report -> [meta + [tool: 'bracken'], report]} - ) + ch_raw_profiles = ch_raw_profiles.mix(BRACKEN_BRACKEN.out.reports) } From 7bd6112133ff3ff67772728243db29b5cabf4607 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 21 Oct 2022 10:18:11 +0200 Subject: [PATCH 029/132] docs: describe Bracken database usage --- docs/usage.md | 17 +++++++++++++---- 1 file changed, 13 insertions(+), 4 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 4f4c1ac..dd80eda 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -74,13 +74,13 @@ The pipeline takes the locations and specific profiling parameters of the tool o > ⚠️ nf-core/taxprofiler does not provide any databases by default, nor does it currently generate them for you. This must be performed manually by the user. See below for more information of the expected database files. -An example database sheet can look as follows, where 4 tools are being used, and `malt` and `kraken2` will be used against two databases each. +An example database sheet can look as follows, where 4 tools are being used, and `malt` and `kraken2` will be used against two databases each. This is because specifying `bracken` implies first running `kraken2` on the same database. ```console tool,db_name,db_params,db_path malt,malt85,-id 85,///malt/testdb-malt/ malt,malt95,-id 90,///malt/testdb-malt.tar.gz -kraken2,db1,,///kraken2/testdb-kraken2.tar.gz +bracken,db1,,///bracken/testdb-bracken.tar.gz kraken2,db2,--quick,///kraken2/testdb-kraken2.tar.gz centrifuge,db1,,///centrifuge/minigut_cf.tar.gz metaphlan3,db1,,///metaphlan3/metaphlan_database/ @@ -91,8 +91,8 @@ Column specifications are as follows: | Column | Description | | ----------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `tool` | Taxonomic profiling tool (supported by nf-core/taxprofiler) that the database has been indexed for [required]. | -| `db_name` | A unique name of the particular database [required]. | +| `tool` | Taxonomic profiling tool (supported by nf-core/taxprofiler) that the database has been indexed for [required]. Please note that `bracken` also implies running `kraken2` on the same database. | +| `db_name` | A unique name per tool for the particular database [required]. Please note that names need to be unique across both `kraken2` and `bracken` as well. | | `db_params` | Any parameters of the given taxonomic profiler that you wish to specify that the taxonomic profiling tool should use when profiling against this specific. Can be empty to use taxonomic profiler defaults. Must not be surrounded by quotes [required]. We generally do not recommend specifying parameters here that turn on/off saving of output files or specifying particular file extensions - this should be already addressed via pipeline parameters. | | `db_path` | Path to the database. Can either be a path to a directory containing the database index files or a `.tar.gz` file which contains the compressed database directory with the same name as the tar archive, minus `.tar.gz` [required]. | @@ -116,6 +116,15 @@ Expected (uncompressed) database files for each tool are as follows: - `opts.k2d` - `hash.k2d` - `taxo.k2d` +- **Bracken** output of a combined `kraken2-` and `bracken-build` process. Please see the [documentation on Bracken](https://github.com/jenniferlu717/Bracken#running-bracken-easy-version) for details. The output is a directory containing files per expected sequencing read length similarly to: + - `hash.k2d` + - `opts.k2d` + - `taxo.k2d` + - `database.kraken` + - `database100mers.kmer_distrib` + - `database100mers.kraken` + - `database150mers.kmer_distrib` + - `database150mers.kraken` - **Centrifuge** output of `centrifuge-build`. A directory containing: - `..cf` - `..cf` From 60e369aa5c7601a46e2ea2d6dddbe457f37b9f42 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 21 Oct 2022 10:47:51 +0200 Subject: [PATCH 030/132] fix: set default run bracken to false --- conf/test_motus.config | 1 + nextflow.config | 2 +- 2 files changed, 2 insertions(+), 1 deletion(-) diff --git a/conf/test_motus.config b/conf/test_motus.config index d5eb8f8..d167b94 100644 --- a/conf/test_motus.config +++ b/conf/test_motus.config @@ -33,6 +33,7 @@ params { hostremoval_reference = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' run_kaiju = false run_kraken2 = false + run_bracken = false run_malt = false run_metaphlan3 = false run_centrifuge = false diff --git a/nextflow.config b/nextflow.config index 4f32a00..24ccab3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -117,7 +117,7 @@ params { kraken2_save_minimizers = false // Bracken - run_bracken = true + run_bracken = false // centrifuge run_centrifuge = false From ca7dc308cb89d6e8ba110de5c96ce1641ff8940a Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Fri, 21 Oct 2022 16:36:35 +0200 Subject: [PATCH 031/132] Apply suggestions from code review Co-authored-by: James A. Fellows Yates --- docs/usage.md | 2 +- subworkflows/local/profiling.nf | 4 +++- 2 files changed, 4 insertions(+), 2 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index dd80eda..56f9123 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -92,7 +92,7 @@ Column specifications are as follows: | Column | Description | | ----------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `tool` | Taxonomic profiling tool (supported by nf-core/taxprofiler) that the database has been indexed for [required]. Please note that `bracken` also implies running `kraken2` on the same database. | -| `db_name` | A unique name per tool for the particular database [required]. Please note that names need to be unique across both `kraken2` and `bracken` as well. | +| `db_name` | A unique name per tool for the particular database [required]. Please note that names need to be unique across both `kraken2` and `bracken` as well, even if re-using the same database. | | `db_params` | Any parameters of the given taxonomic profiler that you wish to specify that the taxonomic profiling tool should use when profiling against this specific. Can be empty to use taxonomic profiler defaults. Must not be surrounded by quotes [required]. We generally do not recommend specifying parameters here that turn on/off saving of output files or specifying particular file extensions - this should be already addressed via pipeline parameters. | | `db_path` | Path to the database. Can either be a path to a directory containing the database index files or a `.tar.gz` file which contains the compressed database directory with the same name as the tar archive, minus `.tar.gz` [required]. | diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index e76db6c..e6272ac 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -41,7 +41,7 @@ workflow PROFILING { .combine(databases) .branch { malt: it[2]['tool'] == 'malt' - kraken2: it[2]['tool'] == 'kraken2' || it[2]['tool'] == 'bracken' + kraken2: it[2]['tool'] == 'kraken2' || it[2]['tool'] == 'bracken' // to reuse the kraken module to produce the input data for bracken metaphlan3: it[2]['tool'] == 'metaphlan3' centrifuge: it[2]['tool'] == 'centrifuge' kaiju: it[2]['tool'] == 'kaiju' @@ -134,6 +134,7 @@ workflow PROFILING { ch_raw_profiles = ch_raw_profiles.mix( KRAKEN2_KRAKEN2.out.report // Set the tool to be strictly 'kraken2' instead of potentially 'bracken' for downstream use. + // Will remain distinct from 'pure' Kraken2 results due to distinct database names in file names. .map { meta, report -> [meta + [tool: 'kraken2'], report]} ) @@ -141,6 +142,7 @@ workflow PROFILING { if ( params.run_kraken2 && params.run_bracken ) { + // remove files from 'pure' kraken2 runs, so only those aligned against bracken2 kraken database are taken for brakcen def ch_input_for_bracken = KRAKEN2_KRAKEN2.out.report .filter { meta, report -> meta['tool'] == 'bracken' } From d3d4297ca932ff1b33cc60827995af673ae832f5 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Mon, 24 Oct 2022 16:28:07 +0200 Subject: [PATCH 032/132] Apply review suggestions --- workflows/taxprofiler.nf | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/workflows/taxprofiler.nf b/workflows/taxprofiler.nf index 905cbd9..1ac519c 100644 --- a/workflows/taxprofiler.nf +++ b/workflows/taxprofiler.nf @@ -257,12 +257,12 @@ workflow TAXPROFILER { ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - if (!params.preprocessing_qc_tool == 'falco') { + if ( params.preprocessing_qc_tool == 'falco' ) { + ch_multiqc_files = ch_multiqc_files.mix(FALCO.out.txt.collect{it[1]}.ifEmpty([])) + } else { ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) } - else { - ch_multiqc_files = ch_multiqc_files.mix(FALCO.out.txt.collect{it[1]}.ifEmpty([])) - } + if (params.perform_shortread_qc) { ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_PREPROCESSING.out.mqc.collect{it[1]}.ifEmpty([]) ) From 00038db6345d0492bc0a1a4ce0dacdf86dbd836e Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 25 Oct 2022 08:51:58 +0000 Subject: [PATCH 033/132] [automated] Fix linting with Prettier --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 56f9123..ada5a2d 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -92,7 +92,7 @@ Column specifications are as follows: | Column | Description | | ----------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | | `tool` | Taxonomic profiling tool (supported by nf-core/taxprofiler) that the database has been indexed for [required]. Please note that `bracken` also implies running `kraken2` on the same database. | -| `db_name` | A unique name per tool for the particular database [required]. Please note that names need to be unique across both `kraken2` and `bracken` as well, even if re-using the same database. | +| `db_name` | A unique name per tool for the particular database [required]. Please note that names need to be unique across both `kraken2` and `bracken` as well, even if re-using the same database. | | `db_params` | Any parameters of the given taxonomic profiler that you wish to specify that the taxonomic profiling tool should use when profiling against this specific. Can be empty to use taxonomic profiler defaults. Must not be surrounded by quotes [required]. We generally do not recommend specifying parameters here that turn on/off saving of output files or specifying particular file extensions - this should be already addressed via pipeline parameters. | | `db_path` | Path to the database. Can either be a path to a directory containing the database index files or a `.tar.gz` file which contains the compressed database directory with the same name as the tar archive, minus `.tar.gz` [required]. | From 8c3e80b6ea85b316b5f476c016a068180a09ffdd Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Tue, 25 Oct 2022 11:13:26 +0200 Subject: [PATCH 034/132] Add helptest to nextflow_schema,add versions to longread_preprocessing --- .github/workflows/ci.yml | 1 - docs/usage.md | 2 +- nextflow_schema.json | 4 +++- subworkflows/local/longread_preprocessing.nf | 2 ++ 4 files changed, 6 insertions(+), 3 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 12185fe..862e072 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -23,7 +23,6 @@ jobs: - "21.10.3" - "latest-everything" parameters: - - "--preprocessing_qc_tool fastqc" - "--preprocessing_qc_tool falco" - "--perform_longread_qc false" - "--perform_shortread_qc false" diff --git a/docs/usage.md b/docs/usage.md index 4a44cf4..32fec4d 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -168,7 +168,7 @@ work # Directory containing the nextflow working files ### Sequencing quality control -nf-core taxprofiler offers [`Falco`](https://github.com/smithlabcode/falco] as an alternative option to [`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/). +nf-core taxprofiler offers [`falco`](https://github.com/smithlabcode/falco] as an alternative option to [`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/). ### Preprocessing Steps diff --git a/nextflow_schema.json b/nextflow_schema.json index ff2505f..aec2b3f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -712,7 +712,9 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": ["fastqc", "falco"] + "enum": ["fastqc", "falco"], + "help_text": "Falco is aimed at being a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", + "description": "By default, the pipeline utilizes FastQC tool for quality control of raw sequencing reads" } } } diff --git a/subworkflows/local/longread_preprocessing.nf b/subworkflows/local/longread_preprocessing.nf index d16db3f..3da49ce 100644 --- a/subworkflows/local/longread_preprocessing.nf +++ b/subworkflows/local/longread_preprocessing.nf @@ -56,10 +56,12 @@ workflow LONGREAD_PREPROCESSING { if (params.preprocessing_qc_tool == 'fastqc') { FASTQC_PROCESSED ( ch_processed_reads ) + ch_versions = ch_versions.mix( FASTQC_PROCESSED.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( FASTQC_PROCESSED.out.zip ) } else if (params.preprocessing_qc_tool == 'falco') { FALCO_PROCESSED ( ch_processed_reads ) + ch_versions = ch_versions.mix( FALCO_PROCESSED.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( FALCO_PROCESSED.out.txt ) } From 7b1a53b06267ecb672a785b7c72a4e6f3cf60b26 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Tue, 25 Oct 2022 11:19:08 +0200 Subject: [PATCH 035/132] Update nextflow_schema.json Co-authored-by: James A. Fellows Yates --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index aec2b3f..225e05d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -713,7 +713,7 @@ "type": "string", "default": "fastqc", "enum": ["fastqc", "falco"], - "help_text": "Falco is aimed at being a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", + "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "By default, the pipeline utilizes FastQC tool for quality control of raw sequencing reads" } } From de6816ec490cce6ba93c86a557c1800acf6ff5d9 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Tue, 25 Oct 2022 11:19:16 +0200 Subject: [PATCH 036/132] Update nextflow_schema.json Co-authored-by: James A. Fellows Yates --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 225e05d..4479f54 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -714,7 +714,7 @@ "default": "fastqc", "enum": ["fastqc", "falco"], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", - "description": "By default, the pipeline utilizes FastQC tool for quality control of raw sequencing reads" + "description": "Specify the tool used for quality control of raw sequencing reads" } } } From 36e101a78b2f87b1aef1a5b05f65b6b730f5ad9d Mon Sep 17 00:00:00 2001 From: Mahwash Jamy Date: Tue, 25 Oct 2022 09:26:33 +0000 Subject: [PATCH 037/132] Install krakenuniq module --- .../krakenuniq/preloadedkrakenuniq/main.nf | 118 ++++++++++++++++++ .../krakenuniq/preloadedkrakenuniq/meta.yml | 77 ++++++++++++ 2 files changed, 195 insertions(+) create mode 100644 modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf create mode 100644 modules/nf-core/krakenuniq/preloadedkrakenuniq/meta.yml diff --git a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf new file mode 100644 index 0000000..a355c13 --- /dev/null +++ b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf @@ -0,0 +1,118 @@ +process KRAKENUNIQ_PRELOADEDKRAKENUNIQ { + tag "$meta.id" + label 'process_high' + + conda (params.enable_conda ? "bioconda::krakenuniq=1.0.0" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/krakenuniq:1.0.0--pl5321h19e8d03_0': + 'quay.io/biocontainers/krakenuniq:1.0.0--pl5321h19e8d03_0' }" + + input: + tuple val(meta), path(fastqs) + path db + val ram_chunk_size + val save_output_fastqs + val report_file + val save_output + + output: + tuple val(meta), path('*.classified{.,_}*') , optional:true, emit: classified_reads_fastq + tuple val(meta), path('*.unclassified{.,_}*') , optional:true, emit: unclassified_reads_fastq + tuple val(meta), path('*classified.txt') , optional:true, emit: classified_assignment + tuple val(meta), path('*report.txt') , emit: report + + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args ?: '' + + def paired = meta.single_end ? "" : "--paired" + def classified = meta.single_end ? '"\$PREFIX".classified.fastq' : '"\$PREFIX".classified#.fastq' + def unclassified = meta.single_end ? '"\$PREFIX".unclassified.fastq' : '"\$PREFIX".unclassified#.fastq' + def classified_option = save_output_fastqs ? "--classified-out ${classified}" : "" + def unclassified_option = save_output_fastqs ? "--unclassified-out ${unclassified}" : "" + def output_option = save_output ? '--output "\$PREFIX".krakenuniq.classified.txt' : "" + def report = report_file ? '--report-file "\$PREFIX".krakenuniq.report.txt' : "" + def compress_reads_command = save_output_fastqs ? "gzip --no-name *.fastq" : "" + + """ + krakenuniq \\ + $args \\ + --db $db \\ + --preload $ram_chunk_size \\ + --threads $task.cpus + + for fastq in ${fastqs.join(' ')}; do \\ + PREFIX=\$(echo \$fastq) + krakenuniq \\ + --db $db \\ + --threads $task.cpus \\ + $report \\ + $output_option \\ + $unclassified_option \\ + $classified_option \\ + $output_option \\ + $paired \\ + $args2 \\ + \$fastq + done + + $compress_reads_command + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + krakenuniq: \$(echo \$(krakenuniq --version 2>&1) | sed 's/^.*KrakenUniq version //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def args2 = task.ext.args ?: '' + + def paired = meta.single_end ? "" : "--paired" + def classified = meta.single_end ? '"\$PREFIX".classified.fastq' : '"\$PREFIX".classified#.fastq' + def unclassified = meta.single_end ? '"\$PREFIX".unclassified.fastq' : '"\$PREFIX".unclassified#.fastq' + def classified_option = save_output_fastqs ? "--classified-out ${classified}" : "" + def unclassified_option = save_output_fastqs ? "--unclassified-out ${unclassified}" : "" + def output_option = save_output ? '--output "\$PREFIX".krakenuniq.classified.txt' : "" + def report = report_file ? '--report-file "\$PREFIX".krakenuniq.report.txt' : "" + def compress_reads_command = save_output_fastqs ? "echo 'gzip --no-name *.fastq'" : "" + """ + echo "krakenuniq \\ + $args \\ + --db $db \\ + --preload $ram_chunk_size \\ + --threads $task.cpus" + + for fastq in ${fastqs.join(' ')}; do \\ + PREFIX=\$(echo \$fastq) + echo "krakenuniq \\ + --db $db \\ + --threads $task.cpus \\ + $report \\ + $output_option \\ + $unclassified_option \\ + $classified_option \\ + $output_option \\ + $paired \\ + $args2 \\ + \$fastq" + + touch "\$PREFIX".classified.fastq.gz + touch "\$PREFIX".krakenuniq.classified.txt + touch "\$PREFIX".krakenuniq.report.txt + touch "\$PREFIX".unclassified.fastq.gz + done + + $compress_reads_command + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + krakenuniq: \$(echo \$(krakenuniq --version 2>&1) | sed 's/^.*KrakenUniq version //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/krakenuniq/preloadedkrakenuniq/meta.yml b/modules/nf-core/krakenuniq/preloadedkrakenuniq/meta.yml new file mode 100644 index 0000000..de788af --- /dev/null +++ b/modules/nf-core/krakenuniq/preloadedkrakenuniq/meta.yml @@ -0,0 +1,77 @@ +name: "krakenuniq_preloadedkrakenuniq" +description: Classifies metagenomic sequence data using unique k-mer counts +keywords: + - classify + - metagenomics + - kmers + - fastq + - db +tools: + - "krakenuniq": + description: "Metagenomics classifier with unique k-mer counting for more specific results" + homepage: https://github.com/fbreitwieser/krakenuniq + documentation: https://github.com/fbreitwieser/krakenuniq + doi: 10.1186/s13059-018-1568-0 + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fastqs: + type: file + description: List of input FastQ files + - db: + type: directory + description: KrakenUniq database + - ram_chunk_size: + type: val + description: Amount of maximum amount of RAM each chunk of database that should be loaded at any one time + pattern: "*GB" + - save_output_fastqs: + type: boolean + description: | + If true, optional commands are added to save classified and unclassified reads + as fastq files + - save_reads_assignment: + type: boolean + description: | + If true, an optional command is added to save a file reporting the taxonomic + classification of each input read +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - classified_reads_fastq: + type: file + description: | + Reads classified as belonging to any of the taxa + on the KrakenUniq database. + pattern: "*{fastq.gz}" + - unclassified_reads_fastq: + type: file + description: | + Reads not classified to any of the taxa + on the KrakenUniq database. + pattern: "*{fastq.gz}" + - classified_assignment: + type: file + description: | + KrakenUniq output file indicating the taxonomic assignment of + each input read ## DOUBLE CHECK!! + - report: + type: file + description: | + KrakenUniq report containing stats about classified + and not classifed reads. + pattern: "*.{report.txt}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@mjamy" From 86ca71cfd4399147d7dbfac927f96c3867e3f003 Mon Sep 17 00:00:00 2001 From: Mahwash Jamy Date: Tue, 25 Oct 2022 09:45:05 +0000 Subject: [PATCH 038/132] include krakenuniq in profiling subworkflow --- modules.json | 4 ++++ subworkflows/local/profiling.nf | 18 ++++++++++++++++++ 2 files changed, 22 insertions(+) diff --git a/modules.json b/modules.json index 10d6c74..2331ef8 100644 --- a/modules.json +++ b/modules.json @@ -89,6 +89,10 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" }, + "krakenuniq/preloadedkrakenuniq": { + "branch": "master", + "git_sha": "13b9d4854593c03e5e25e8a8f47462542c2c0dd4" + }, "krona/ktimporttaxonomy": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 11c4a72..6432829 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -11,6 +11,7 @@ include { METAPHLAN3_METAPHLAN3 } from '../../modules/nf-core/me include { KAIJU_KAIJU } from '../../modules/nf-core/kaiju/kaiju/main' include { DIAMOND_BLASTX } from '../../modules/nf-core/diamond/blastx/main' include { MOTUS_PROFILE } from '../../modules/nf-core/motus/profile/main' +include { KRAKENUNIQ_PRELOADEDKRAKENUNIQ } from '../../modules/nf-core/krakenuniq/preloadedkrakenuniq/main' workflow PROFILING { take: @@ -45,6 +46,7 @@ workflow PROFILING { kaiju: it[2]['tool'] == 'kaiju' diamond: it[2]['tool'] == 'diamond' motus: it[2]['tool'] == 'motus' + krakenuniq: it[2]['tool'] == 'krakenuniq' unknown: true } @@ -228,6 +230,22 @@ workflow PROFILING { ch_multiqc_files = ch_multiqc_files.mix( MOTUS_PROFILE.out.log ) } + if ( params.run_krakenuniq ) { + ch_input_for_krakenuniq = ch_input_for_profiling.krakenuniq + .multiMap { + it -> + reads: [ it[0] + it[2], it[1] ] + db: it[3] + } + + KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads, ch_input_for_krakenuniq.db, params.krakenuniq_save_reads, params.krakenuniq_save_readclassification ) + ch_multiqc_files = ch_multiqc_files.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.report ) + ch_versions = ch_versions.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.versions.first() ) + ch_raw_classifications = ch_raw_classifications.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.classified_assignment ) + ch_raw_profiles = ch_raw_profiles.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.report ) + + } + emit: classifications = ch_raw_classifications profiles = ch_raw_profiles // channel: [ val(meta), [ reads ] ] - should be text files or biom From f4717be9abcdb71771101936b9b1881142478feb Mon Sep 17 00:00:00 2001 From: Mahwash Jamy Date: Tue, 25 Oct 2022 09:50:44 +0000 Subject: [PATCH 039/132] add krakenuniq to modules.config --- conf/modules.config | 10 ++++++++++ 1 file changed, 10 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index d2a0051..252a8fe 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -295,6 +295,16 @@ process { ] } + withName: KRAKENUNIQ_PRELOADEDKRAKENUNIQ { + ext.args = { "${meta.db_params}" } + ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } + publishDir = [ + path: { "${params.outdir}/krakenuniq/${meta.db_name}/" }, + mode: params.publish_dir_mode, + pattern: '*.{txt,report,fastq.gz}' + ] + } + withName: KRONA_CLEANUP { ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ From 0de1ac53561d490e35a500b0e18da3e37a913907 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Thu, 27 Oct 2022 10:14:57 +0200 Subject: [PATCH 040/132] docs: add Bracken reference --- CITATIONS.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/CITATIONS.md b/CITATIONS.md index 1ce4ec2..ec03fc4 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -36,6 +36,10 @@ > Wood, Derrick E., Jennifer Lu, and Ben Langmead. 2019. Improved Metagenomic Analysis with Kraken 2. Genome Biology 20 (1): 257. doi: 10.1186/s13059-019-1891-0. +- [Bracken](https://doi.org/10.7717/peerj-cs.104) + + > Lu, J., Breitwieser, F. P., Thielen, P., & Salzberg, S. L. (2017). Bracken: Estimating species abundance in metagenomics data. PeerJ Computer Science, 3, e104. doi: 10.7717/peerj-cs.104 + - [Krona](https://doi.org/10.1186/1471-2105-12-385) > Ondov, Brian D., Nicholas H. Bergman, and Adam M. Phillippy. 2011. Interactive metagenomic visualization in a Web browser. BMC Bioinformatics 12 (1): 385. doi: 10.1186/1471-2105-12-385. From dd1a4ff158bff66021138f4f63f5585a477b6618 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Thu, 27 Oct 2022 11:19:14 +0200 Subject: [PATCH 041/132] refactor: only combine same database name [skip ci] --- subworkflows/local/profiling.nf | 25 ++++++++++++++----------- 1 file changed, 14 insertions(+), 11 deletions(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index e6272ac..278e310 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -141,22 +141,25 @@ workflow PROFILING { } if ( params.run_kraken2 && params.run_bracken ) { - - // remove files from 'pure' kraken2 runs, so only those aligned against bracken2 kraken database are taken for brakcen - def ch_input_for_bracken = KRAKEN2_KRAKEN2.out.report + // Remove files from 'pure' kraken2 runs, so only those aligned against Bracken & kraken2 database are used. + def ch_kraken2_output = KRAKEN2_KRAKEN2.out.report .filter { meta, report -> meta['tool'] == 'bracken' } + // If necessary, convert the eight column output to six column output. if (params.kraken2_save_minimizers) { - ch_input_for_bracken = KRAKEN2_STANDARD_REPORT(ch_input_for_bracken).report + ch_kraken2_output = KRAKEN2_STANDARD_REPORT(ch_kraken2_output).report } - ch_input_for_bracken = ch_input_for_bracken - .combine( - databases.filter { meta, db -> - meta['tool'] == 'bracken' - } - ) - .multiMap { meta, report, db_meta, db -> + // Extract the database name to combine by. + def ch_bracken_databases = databases + .filter { meta, db -> meta['tool'] == 'bracken' } + .map { meta, db -> [meta['db_name'], meta, db] } + + // Extract the database name to combine by. + def ch_input_for_bracken = ch_kraken2_output + .map { meta, report -> [meta['db_name'], meta, report] } + .combine(ch_bracken_databases, by: 0) + .multiMap { key, meta, report, db_meta, db -> report: [meta + db_meta, report] db: db } From e658fab430db815bf7614743e3b249c861df15ee Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Thu, 27 Oct 2022 11:50:47 +0200 Subject: [PATCH 042/132] fix: remove `def` No idea why, must have something to do with scope. --- subworkflows/local/profiling.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 278e310..b86c165 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -151,12 +151,12 @@ workflow PROFILING { } // Extract the database name to combine by. - def ch_bracken_databases = databases + ch_bracken_databases = databases .filter { meta, db -> meta['tool'] == 'bracken' } .map { meta, db -> [meta['db_name'], meta, db] } // Extract the database name to combine by. - def ch_input_for_bracken = ch_kraken2_output + ch_input_for_bracken = ch_kraken2_output .map { meta, report -> [meta['db_name'], meta, report] } .combine(ch_bracken_databases, by: 0) .multiMap { key, meta, report, db_meta, db -> From 76a662ecc5e6a63b569b887df52ac41d1946cb7d Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Thu, 27 Oct 2022 12:17:19 +0000 Subject: [PATCH 043/132] Fix adapterremoval adapter conflict and add optional adapter list file --- conf/modules.config | 8 +-- modules.json | 2 +- modules/nf-core/adapterremoval/main.nf | 4 +- nextflow.config | 3 +- nextflow_schema.json | 61 +++++++++++++++---- .../local/shortread_adapterremoval.nf | 5 +- subworkflows/local/shortread_preprocessing.nf | 1 + workflows/taxprofiler.nf | 9 ++- 8 files changed, 69 insertions(+), 24 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index b1d7b87..9a351cc 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -100,8 +100,7 @@ process { withName: ADAPTERREMOVAL_SINGLE { ext.args = [ // trimming options - params.shortread_qc_skipadaptertrim ? "--adapter1 '' --adapter2 ''" : "", - params.shortread_qc_adapter1 ? "--adapter1 ${params.shortread_qc_adapter1}" : "", + params.shortread_qc_skipadaptertrim ? "--adapter1 ''" : params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter1 ? "--adapter1 ${params.shortread_qc_adapter1}" : "", // filtering options "--minlength ${params.shortread_qc_minlength}" ].join(' ').trim() @@ -119,9 +118,8 @@ process { // collapsing options params.shortread_qc_mergepairs ? "--collapse" : "", // trimming options - params.shortread_qc_skipadaptertrim ? "--adapter1 '' --adapter2 ''" : "", - params.shortread_qc_adapter1 ? "--adapter1 ${params.shortread_qc_adapter1}" : "", - params.shortread_qc_adapter2 ? "--adapter2 ${params.shortread_qc_adapter2}" : "", + params.shortread_qc_skipadaptertrim ? "--adapter1 ''" : params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter1 ? "--adapter1 ${params.shortread_qc_adapter1}" : "", // adding adapter list happens at module input channel level + params.shortread_qc_skipadaptertrim ? "--adapter2 ''" : params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter2 ? "--adapter2 ${params.shortread_qc_adapter2}" : "", // filtering options "--minlength ${params.shortread_qc_minlength}" ].join(' ').trim() diff --git a/modules.json b/modules.json index aa77c4e..ab32eec 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "adapterremoval": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + "git_sha": "ce7cf27e377fdacf7ebe8e75903ec70405ea1659" }, "bbmap/bbduk": { "branch": "master", diff --git a/modules/nf-core/adapterremoval/main.nf b/modules/nf-core/adapterremoval/main.nf index 0e17c05..643c141 100644 --- a/modules/nf-core/adapterremoval/main.nf +++ b/modules/nf-core/adapterremoval/main.nf @@ -34,7 +34,7 @@ process ADAPTERREMOVAL { AdapterRemoval \\ --file1 $reads \\ $args \\ - $adapterlist \\ + $list \\ --basename ${prefix} \\ --threads ${task.cpus} \\ --seed 42 \\ @@ -61,7 +61,7 @@ process ADAPTERREMOVAL { --file1 ${reads[0]} \\ --file2 ${reads[1]} \\ $args \\ - $adapterlist \\ + $list \\ --basename ${prefix} \\ --threads $task.cpus \\ --seed 42 \\ diff --git a/nextflow.config b/nextflow.config index b69948e..9bf7f83 100644 --- a/nextflow.config +++ b/nextflow.config @@ -59,9 +59,9 @@ params { // Databases databases = null + // FASTQ preprocessing preprocessing_qc_tool = 'fastqc' - // FASTQ preprocessing perform_shortread_qc = false shortread_qc_tool = 'fastp' shortread_qc_skipadaptertrim = false @@ -69,6 +69,7 @@ params { shortread_qc_excludeunmerged = false shortread_qc_adapter1 = null shortread_qc_adapter2 = null + shortread_qc_adapterlist = null // TODO Add for FASTP shortread_qc_minlength = 15 perform_longread_qc = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 4479f54..59131b6 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,11 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir", "databases"], + "required": [ + "input", + "databases", + "outdir" + ], "properties": { "input": { "type": "string", @@ -80,7 +84,10 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": ["fastp", "adapterremoval"], + "enum": [ + "fastp", + "adapterremoval" + ], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -104,6 +111,12 @@ "description": "Specify adapter 2 nucleotide sequence", "help_text": "Specify a custom reverse or R2 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT`" }, + "shortread_qc_adapterlist": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-list-ul", + "help_text": "This affects AdapterRemoval. It will replace default adapters or whatever is supplied in `--shortread_qc_adapter{1,2}`.\n\nThis allows you to mix libraries built with different adapters into one run.\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--adapter-list`." + }, "shortread_qc_mergepairs": { "type": "boolean", "fa_icon": "fas fa-toggle-on", @@ -133,7 +146,11 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], + "enum": [ + "bbduk", + "prinseqplusplus", + "fastp" + ], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -167,7 +184,10 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": ["entropy", "dust"], + "enum": [ + "entropy", + "dust" + ], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -341,7 +361,15 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "enum": [ + "blast", + "xml", + "txt", + "daa", + "sam", + "tsv", + "paf" + ], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -360,7 +388,14 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], + "enum": [ + "phylum", + "class", + "order", + "family", + "genus", + "species" + ], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -555,7 +590,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { @@ -711,10 +753,7 @@ "properties": { "preprocessing_qc_tool": { "type": "string", - "default": "fastqc", - "enum": ["fastqc", "falco"], - "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", - "description": "Specify the tool used for quality control of raw sequencing reads" + "default": "fastqc" } } } diff --git a/subworkflows/local/shortread_adapterremoval.nf b/subworkflows/local/shortread_adapterremoval.nf index a5a43fe..6567007 100644 --- a/subworkflows/local/shortread_adapterremoval.nf +++ b/subworkflows/local/shortread_adapterremoval.nf @@ -10,6 +10,7 @@ workflow SHORTREAD_ADAPTERREMOVAL { take: reads // [[meta], [reads]] + adapterlist // file main: ch_versions = Channel.empty() @@ -21,8 +22,8 @@ workflow SHORTREAD_ADAPTERREMOVAL { paired: !it[0].single_end } - ADAPTERREMOVAL_SINGLE ( ch_input_for_adapterremoval.single, [] ) - ADAPTERREMOVAL_PAIRED ( ch_input_for_adapterremoval.paired, [] ) + ADAPTERREMOVAL_SINGLE ( ch_input_for_adapterremoval.single, adapterlist ) + ADAPTERREMOVAL_PAIRED ( ch_input_for_adapterremoval.paired, adapterlist ) /* * Due to the ~slightly~ very ugly output implementation of the current AdapterRemoval2 version, each file diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index f2cd738..6e14423 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -11,6 +11,7 @@ include { FALCO as FALCO_PROCESSED } from '../../modules/nf-core/falco/main' workflow SHORTREAD_PREPROCESSING { take: reads // [ [ meta ], [ reads ] ] + adapterlist // file main: ch_versions = Channel.empty() diff --git a/workflows/taxprofiler.nf b/workflows/taxprofiler.nf index 1ac519c..e6ccc16 100644 --- a/workflows/taxprofiler.nf +++ b/workflows/taxprofiler.nf @@ -12,7 +12,8 @@ WorkflowTaxprofiler.initialise(params, log) // TODO nf-core: Add all file path parameters for the pipeline to the list below // Check input path parameters to see if they exist def checkPathParamList = [ params.input, params.databases, params.hostremoval_reference, - params.shortread_hostremoval_index, params.multiqc_config + params.shortread_hostremoval_index, params.multiqc_config, + params.shortread_qc_adapterlist ] for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } @@ -102,6 +103,7 @@ workflow TAXPROFILER { ch_versions = Channel.empty() ch_multiqc_logo= Channel.fromPath("$projectDir/docs/images/nf-core-taxprofiler_logo_custom_light.png") + ch_adapterlist_for_shortreadqc = params.shortread_qc_adapterlist ? file(params.shortread_qc_adapterlist) : [] /* SUBWORKFLOW: Read in samplesheet, validate and stage input files @@ -131,8 +133,11 @@ workflow TAXPROFILER { /* SUBWORKFLOW: PERFORM PREPROCESSING */ + + ch_adapterlist_for_shortreadqc + if ( params.perform_shortread_qc ) { - ch_shortreads_preprocessed = SHORTREAD_PREPROCESSING ( INPUT_CHECK.out.fastq ).reads + ch_shortreads_preprocessed = SHORTREAD_PREPROCESSING ( INPUT_CHECK.out.fastq, ch_adapterlist_for_shortreadqc ).reads ch_versions = ch_versions.mix( SHORTREAD_PREPROCESSING.out.versions ) } else { ch_shortreads_preprocessed = INPUT_CHECK.out.fastq From 733d3dc8c57078a7c2032ef028429aefc552e3de Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Thu, 27 Oct 2022 14:19:28 +0200 Subject: [PATCH 044/132] Apply suggestions from code review --- nextflow_schema.json | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 59131b6..5c60775 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -753,7 +753,10 @@ "properties": { "preprocessing_qc_tool": { "type": "string", - "default": "fastqc" + "default": "fastqc", + "enum": ["fastqc", "falco"], + "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", + "description": "Specify the tool used for quality control of raw sequencing reads" } } } From a402ee3be200089757096207dba17cb1b4ffb5a6 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 27 Oct 2022 12:20:08 +0000 Subject: [PATCH 045/132] [automated] Fix linting with Prettier --- nextflow_schema.json | 50 +++++++------------------------------------- 1 file changed, 7 insertions(+), 43 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 5c60775..04ba938 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,11 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "databases", - "outdir" - ], + "required": ["input", "databases", "outdir"], "properties": { "input": { "type": "string", @@ -84,10 +80,7 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], + "enum": ["fastp", "adapterremoval"], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -146,11 +139,7 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], + "enum": ["bbduk", "prinseqplusplus", "fastp"], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -184,10 +173,7 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": [ - "entropy", - "dust" - ], + "enum": ["entropy", "dust"], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -361,15 +347,7 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -388,14 +366,7 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], + "enum": ["phylum", "class", "order", "family", "genus", "species"], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -590,14 +561,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { From 08d61065e5fe4bbae15b93690bf77331be89785e Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Thu, 27 Oct 2022 12:23:27 +0000 Subject: [PATCH 046/132] Add adapterlist to AR2 subworkflow --- subworkflows/local/shortread_preprocessing.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index 6e14423..d381d34 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -22,7 +22,7 @@ workflow SHORTREAD_PREPROCESSING { ch_versions = ch_versions.mix( SHORTREAD_FASTP.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_FASTP.out.mqc ) } else if ( params.shortread_qc_tool == "adapterremoval" ) { - ch_processed_reads = SHORTREAD_ADAPTERREMOVAL ( reads ).reads + ch_processed_reads = SHORTREAD_ADAPTERREMOVAL ( reads, adapterlist ).reads ch_versions = ch_versions.mix( SHORTREAD_ADAPTERREMOVAL.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_ADAPTERREMOVAL.out.mqc ) } else { From 1bd6dbfe58b16d3115ff214295dd6835a6a8f4d5 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Fri, 28 Oct 2022 11:40:50 +0200 Subject: [PATCH 047/132] Add samtools stats for long-reads --- assets/multiqc_config.yml | 1 + modules.json | 8 ++++ modules/nf-core/samtools/index/main.nf | 48 ++++++++++++++++++++ modules/nf-core/samtools/index/meta.yml | 53 ++++++++++++++++++++++ modules/nf-core/samtools/stats/main.nf | 49 ++++++++++++++++++++ modules/nf-core/samtools/stats/meta.yml | 53 ++++++++++++++++++++++ subworkflows/local/longread_hostremoval.nf | 15 ++++++ workflows/taxprofiler.nf | 4 ++ 8 files changed, 231 insertions(+) create mode 100644 modules/nf-core/samtools/index/main.nf create mode 100644 modules/nf-core/samtools/index/meta.yml create mode 100644 modules/nf-core/samtools/stats/main.nf create mode 100644 modules/nf-core/samtools/stats/meta.yml diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index e2b5a6e..e4a04a9 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -21,6 +21,7 @@ run_modules: - adapterRemoval - fastp - bowtie2 + - samtools - kraken - malt - custom_content diff --git a/modules.json b/modules.json index bcf2567..be3d193 100644 --- a/modules.json +++ b/modules.json @@ -153,6 +153,14 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" }, + "samtools/index": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "samtools/stats": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, "samtools/view": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf new file mode 100644 index 0000000..e04e63e --- /dev/null +++ b/modules/nf-core/samtools/index/main.nf @@ -0,0 +1,48 @@ +process SAMTOOLS_INDEX { + tag "$meta.id" + label 'process_low' + + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + + input: + tuple val(meta), path(input) + + output: + tuple val(meta), path("*.bai") , optional:true, emit: bai + tuple val(meta), path("*.csi") , optional:true, emit: csi + tuple val(meta), path("*.crai"), optional:true, emit: crai + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + """ + samtools \\ + index \\ + -@ ${task.cpus-1} \\ + $args \\ + $input + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + """ + touch ${input}.bai + touch ${input}.crai + touch ${input}.csi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml new file mode 100644 index 0000000..e5cadbc --- /dev/null +++ b/modules/nf-core/samtools/index/meta.yml @@ -0,0 +1,53 @@ +name: samtools_index +description: Index SAM/BAM/CRAM file +keywords: + - index + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - crai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - csi: + type: file + description: CSI index file + pattern: "*.{csi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@ewels" + - "@maxulysse" diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf new file mode 100644 index 0000000..9b0c386 --- /dev/null +++ b/modules/nf-core/samtools/stats/main.nf @@ -0,0 +1,49 @@ +process SAMTOOLS_STATS { + tag "$meta.id" + label 'process_single' + + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + + input: + tuple val(meta), path(input), path(input_index) + path fasta + + output: + tuple val(meta), path("*.stats"), emit: stats + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def reference = fasta ? "--reference ${fasta}" : "" + """ + samtools \\ + stats \\ + --threads ${task.cpus} \\ + ${reference} \\ + ${input} \\ + > ${prefix}.stats + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.stats + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml new file mode 100644 index 0000000..cac50b1 --- /dev/null +++ b/modules/nf-core/samtools/stats/meta.yml @@ -0,0 +1,53 @@ +name: samtools_stats +description: Produces comprehensive statistics from SAM/BAM/CRAM file +keywords: + - statistics + - counts + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: BAM/CRAM file from alignment + pattern: "*.{bam,cram}" + - input_index: + type: file + description: BAI/CRAI file from alignment + pattern: "*.{bai,crai}" + - fasta: + type: optional file + description: Reference file the CRAM was created with + pattern: "*.{fasta,fa}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - stats: + type: file + description: File containing samtools stats output + pattern: "*.{stats}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@FriederikeHanssen" diff --git a/subworkflows/local/longread_hostremoval.nf b/subworkflows/local/longread_hostremoval.nf index 82ea8ca..fb79e12 100644 --- a/subworkflows/local/longread_hostremoval.nf +++ b/subworkflows/local/longread_hostremoval.nf @@ -6,6 +6,8 @@ include { MINIMAP2_INDEX } from '../../modules/nf-core/minimap2/inde include { MINIMAP2_ALIGN } from '../../modules/nf-core/minimap2/align/main' include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' include { SAMTOOLS_BAM2FQ } from '../../modules/nf-core/samtools/bam2fq/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' +include { SAMTOOLS_STATS } from '../../modules/nf-core/samtools/stats/main' workflow LONGREAD_HOSTREMOVAL { take: @@ -39,9 +41,22 @@ workflow LONGREAD_HOSTREMOVAL { SAMTOOLS_BAM2FQ ( SAMTOOLS_VIEW.out.bam, false ) ch_versions = ch_versions.mix( SAMTOOLS_BAM2FQ.out.versions.first() ) + SAMTOOLS_INDEX ( SAMTOOLS_VIEW.out.bam ) + ch_versions = ch_versions.mix( SAMTOOLS_INDEX.out.versions.first() ) + + SAMTOOLS_VIEW.out.bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .set { bam_bai } + + SAMTOOLS_STATS ( bam_bai, reference ) + ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) + ch_multiqc_files = ch_multiqc_files.mix( SAMTOOLS_STATS.out.stats ) + emit: + stats = SAMTOOLS_STATS.out.stats //channel: [val(meta), [reads ] ] reads = SAMTOOLS_BAM2FQ.out.reads // channel: [ val(meta), [ reads ] ] versions = ch_versions // channel: [ versions.yml ] + mqc = ch_multiqc_files } diff --git a/workflows/taxprofiler.nf b/workflows/taxprofiler.nf index b456b06..07660ab 100644 --- a/workflows/taxprofiler.nf +++ b/workflows/taxprofiler.nf @@ -281,6 +281,10 @@ workflow TAXPROFILER { ch_multiqc_files = ch_multiqc_files.mix(SHORTREAD_HOSTREMOVAL.out.mqc.collect{it[1]}.ifEmpty([])) } + if (params.perform_longread_hostremoval) { + ch_multiqc_files = ch_multiqc_files.mix(LONGREAD_HOSTREMOVAL.out.mqc.collect{it[1]}.ifEmpty([])) + } + ch_multiqc_files = ch_multiqc_files.mix( PROFILING.out.mqc.collect{it[1]}.ifEmpty([]) ) if ( params.run_profile_standardisation ) { From f87131c7b1c212c3169b0ee3d6462eaa72d2a021 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Fri, 28 Oct 2022 14:10:24 +0200 Subject: [PATCH 048/132] Save samtools stats output --- conf/modules.config | 10 ++++++++++ 1 file changed, 10 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index a4d36fd..397e0c2 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -233,6 +233,16 @@ process { ] } + withName: SAMTOOLS_STATS { + ext.prefix = { "${meta.id}_${meta.run_accession}" } + publishDir = [ + path: { "${params.outdir}/samtools/stats" }, + mode: params.publish_dir_mode, + enabled: params.save_hostremoval_unmapped, + pattern: '*stats' + ] + } + withName: BBMAP_BBDUK { ext.args = [ "entropy=${params.shortread_complexityfilter_entropy}", From 5b99d9024aeafcad5256d534d08c7cd7a2b79d70 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Fri, 28 Oct 2022 14:24:33 +0200 Subject: [PATCH 049/132] Add samtools stats for short-reads --- assets/multiqc_config.yml | 1 + conf/modules.config | 12 +++++ modules.json | 8 ++++ modules/nf-core/samtools/index/main.nf | 48 +++++++++++++++++++ modules/nf-core/samtools/index/meta.yml | 53 +++++++++++++++++++++ modules/nf-core/samtools/stats/main.nf | 49 +++++++++++++++++++ modules/nf-core/samtools/stats/meta.yml | 53 +++++++++++++++++++++ subworkflows/local/shortread_hostremoval.nf | 12 +++++ 8 files changed, 236 insertions(+) create mode 100644 modules/nf-core/samtools/index/main.nf create mode 100644 modules/nf-core/samtools/index/meta.yml create mode 100644 modules/nf-core/samtools/stats/main.nf create mode 100644 modules/nf-core/samtools/stats/meta.yml diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index e2b5a6e..e4a04a9 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -21,6 +21,7 @@ run_modules: - adapterRemoval - fastp - bowtie2 + - samtools - kraken - malt - custom_content diff --git a/conf/modules.config b/conf/modules.config index a4d36fd..476ca11 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -233,6 +233,18 @@ process { ] } + withName: SAMTOOLS_STATS { + ext.prefix = { "${meta.id}_${meta.run_accession}" } + publishDir = [ + path: { "${params.outdir}/samtools/stats" }, + mode: params.publish_dir_mode, + enabled: params.save_hostremoval_unmapped, + pattern: '*stats' + ] + } + + + withName: BBMAP_BBDUK { ext.args = [ "entropy=${params.shortread_complexityfilter_entropy}", diff --git a/modules.json b/modules.json index bcf2567..be3d193 100644 --- a/modules.json +++ b/modules.json @@ -153,6 +153,14 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" }, + "samtools/index": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "samtools/stats": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, "samtools/view": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf new file mode 100644 index 0000000..e04e63e --- /dev/null +++ b/modules/nf-core/samtools/index/main.nf @@ -0,0 +1,48 @@ +process SAMTOOLS_INDEX { + tag "$meta.id" + label 'process_low' + + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + + input: + tuple val(meta), path(input) + + output: + tuple val(meta), path("*.bai") , optional:true, emit: bai + tuple val(meta), path("*.csi") , optional:true, emit: csi + tuple val(meta), path("*.crai"), optional:true, emit: crai + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + """ + samtools \\ + index \\ + -@ ${task.cpus-1} \\ + $args \\ + $input + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + """ + touch ${input}.bai + touch ${input}.crai + touch ${input}.csi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml new file mode 100644 index 0000000..e5cadbc --- /dev/null +++ b/modules/nf-core/samtools/index/meta.yml @@ -0,0 +1,53 @@ +name: samtools_index +description: Index SAM/BAM/CRAM file +keywords: + - index + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - crai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - csi: + type: file + description: CSI index file + pattern: "*.{csi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@ewels" + - "@maxulysse" diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf new file mode 100644 index 0000000..9b0c386 --- /dev/null +++ b/modules/nf-core/samtools/stats/main.nf @@ -0,0 +1,49 @@ +process SAMTOOLS_STATS { + tag "$meta.id" + label 'process_single' + + conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + + input: + tuple val(meta), path(input), path(input_index) + path fasta + + output: + tuple val(meta), path("*.stats"), emit: stats + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def reference = fasta ? "--reference ${fasta}" : "" + """ + samtools \\ + stats \\ + --threads ${task.cpus} \\ + ${reference} \\ + ${input} \\ + > ${prefix}.stats + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.stats + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml new file mode 100644 index 0000000..cac50b1 --- /dev/null +++ b/modules/nf-core/samtools/stats/meta.yml @@ -0,0 +1,53 @@ +name: samtools_stats +description: Produces comprehensive statistics from SAM/BAM/CRAM file +keywords: + - statistics + - counts + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: hhttp://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: BAM/CRAM file from alignment + pattern: "*.{bam,cram}" + - input_index: + type: file + description: BAI/CRAI file from alignment + pattern: "*.{bai,crai}" + - fasta: + type: optional file + description: Reference file the CRAM was created with + pattern: "*.{fasta,fa}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - stats: + type: file + description: File containing samtools stats output + pattern: "*.{stats}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@FriederikeHanssen" diff --git a/subworkflows/local/shortread_hostremoval.nf b/subworkflows/local/shortread_hostremoval.nf index d181a34..a1ed758 100644 --- a/subworkflows/local/shortread_hostremoval.nf +++ b/subworkflows/local/shortread_hostremoval.nf @@ -4,6 +4,8 @@ include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' +include { SAMTOOLS_STATS } from '../../modules/nf-core/samtools/stats/main' workflow SHORTREAD_HOSTREMOVAL { take: @@ -26,7 +28,17 @@ workflow SHORTREAD_HOSTREMOVAL { ch_versions = ch_versions.mix( BOWTIE2_ALIGN.out.versions.first() ) ch_multiqc_files = ch_multiqc_files.mix( BOWTIE2_ALIGN.out.log ) + SAMTOOLS_INDEX ( BOWTIE2_ALIGN.out.bam ) + + bam_bai = BOWTIE2_ALIGN.out.bam + .join(SAMTOOLS_INDEX.out.bai, remainder: true) + + SAMTOOLS_STATS ( bam_bai, reference ) + ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) + ch_multiqc_files = ch_multiqc_files.mix( SAMTOOLS_STATS.out.stats ) + emit: + stats = SAMTOOLS_STATS.out.stats reads = BOWTIE2_ALIGN.out.fastq // channel: [ val(meta), [ reads ] ] versions = ch_versions // channel: [ versions.yml ] mqc = ch_multiqc_files From c4a7d094c8b5327eb6e007540c473fbbc5b94e75 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Fri, 28 Oct 2022 14:33:44 +0200 Subject: [PATCH 050/132] Fix EditorConfig check --- conf/modules.config | 2 -- 1 file changed, 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 476ca11..065f017 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -243,8 +243,6 @@ process { ] } - - withName: BBMAP_BBDUK { ext.args = [ "entropy=${params.shortread_complexityfilter_entropy}", From f1778acf35a6b5e9e00601441e982acf401bdaec Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Mon, 31 Oct 2022 09:31:13 +0100 Subject: [PATCH 051/132] Apply review suggestion --- subworkflows/local/longread_hostremoval.nf | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/subworkflows/local/longread_hostremoval.nf b/subworkflows/local/longread_hostremoval.nf index fb79e12..5f3f746 100644 --- a/subworkflows/local/longread_hostremoval.nf +++ b/subworkflows/local/longread_hostremoval.nf @@ -44,9 +44,8 @@ workflow LONGREAD_HOSTREMOVAL { SAMTOOLS_INDEX ( SAMTOOLS_VIEW.out.bam ) ch_versions = ch_versions.mix( SAMTOOLS_INDEX.out.versions.first() ) - SAMTOOLS_VIEW.out.bam - .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) - .set { bam_bai } + bam_bai = SAMTOOLS_VIEW.out.bam + .join(SAMTOOLS_INDEX.out.bai, remainder: true) SAMTOOLS_STATS ( bam_bai, reference ) ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) From ce611d6efdbde1b6d5fbbd4a9df663d5510458d5 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Mon, 31 Oct 2022 10:04:51 +0100 Subject: [PATCH 052/132] Add bowtie2 mapped reads --- subworkflows/local/shortread_hostremoval.nf | 21 ++++++++++++++++----- 1 file changed, 16 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/shortread_hostremoval.nf b/subworkflows/local/shortread_hostremoval.nf index a1ed758..426442b 100644 --- a/subworkflows/local/shortread_hostremoval.nf +++ b/subworkflows/local/shortread_hostremoval.nf @@ -4,8 +4,9 @@ include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' -include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' -include { SAMTOOLS_STATS } from '../../modules/nf-core/samtools/stats/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' +include { SAMTOOLS_STATS } from '../../modules/nf-core/samtools/stats/main' +include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' workflow SHORTREAD_HOSTREMOVAL { take: @@ -28,9 +29,19 @@ workflow SHORTREAD_HOSTREMOVAL { ch_versions = ch_versions.mix( BOWTIE2_ALIGN.out.versions.first() ) ch_multiqc_files = ch_multiqc_files.mix( BOWTIE2_ALIGN.out.log ) - SAMTOOLS_INDEX ( BOWTIE2_ALIGN.out.bam ) + ch_bowtie2_mapped = BOWTIE2_ALIGN.out.bam + .map { + meta, reads -> + [ meta, reads, [] ] + } - bam_bai = BOWTIE2_ALIGN.out.bam + SAMTOOLS_VIEW ( ch_bowtie2_mapped, [], [] ) + ch_versions = ch_versions.mix( SAMTOOLS_VIEW.out.versions.first() ) + + SAMTOOLS_INDEX ( SAMTOOLS_VIEW.out.bam ) + ch_versions = ch_versions.mix( SAMTOOLS_INDEX.out.versions.first() ) + + bam_bai = SAMTOOLS_VIEW.out.bam .join(SAMTOOLS_INDEX.out.bai, remainder: true) SAMTOOLS_STATS ( bam_bai, reference ) @@ -38,7 +49,7 @@ workflow SHORTREAD_HOSTREMOVAL { ch_multiqc_files = ch_multiqc_files.mix( SAMTOOLS_STATS.out.stats ) emit: - stats = SAMTOOLS_STATS.out.stats + stats = SAMTOOLS_STATS.out.stats reads = BOWTIE2_ALIGN.out.fastq // channel: [ val(meta), [ reads ] ] versions = ch_versions // channel: [ versions.yml ] mqc = ch_multiqc_files From 1d1047374bf4618b0ffb50bc56e2531fb8697aa9 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Mon, 31 Oct 2022 12:09:25 +0100 Subject: [PATCH 053/132] Fix EditorConfig format --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 065f017..a3efaf1 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -222,7 +222,7 @@ process { pattern: '*.bam' ] } - + withName: SAMTOOLS_BAM2FQ { ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ From a4b5fa9f5750820069c90a621640a23a27823d22 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Mon, 31 Oct 2022 12:17:49 +0100 Subject: [PATCH 054/132] Fix trailing space --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index a3efaf1..c2ed5c1 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -233,7 +233,7 @@ process { ] } - withName: SAMTOOLS_STATS { + withName: SAMTOOLS_STATS { ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/samtools/stats" }, From 0e0c55d3b0092a12be6853bcb070fa0de95230a5 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Mon, 31 Oct 2022 12:35:51 +0100 Subject: [PATCH 055/132] Update modules.config --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index c2ed5c1..397e0c2 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -222,7 +222,7 @@ process { pattern: '*.bam' ] } - + withName: SAMTOOLS_BAM2FQ { ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ From febcbfc05719d28e0c43b385322517f194c335c5 Mon Sep 17 00:00:00 2001 From: Mahwash Jamy Date: Mon, 31 Oct 2022 16:07:53 +0000 Subject: [PATCH 056/132] add missing parameters to nextflow.config --- conf/modules.config | 3 ++- nextflow.config | 5 +++++ 2 files changed, 7 insertions(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 8e36c5a..eaa5157 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -335,7 +335,8 @@ process { withName: KRAKENUNIQ_PRELOADEDKRAKENUNIQ { ext.args = { "${meta.db_params}" } - ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } + // one run with multiple samples, so fix ID to just db name to ensure clean log name + ext.prefix = { "${meta.db_name}" } publishDir = [ path: { "${params.outdir}/krakenuniq/${meta.db_name}/" }, mode: params.publish_dir_mode, diff --git a/nextflow.config b/nextflow.config index b369f31..6223db0 100644 --- a/nextflow.config +++ b/nextflow.config @@ -118,6 +118,11 @@ params { kraken2_save_readclassification = false // added directly to module in profiling.nf kraken2_save_minimizers = false + //krakenuniq + run_krakenuniq = false + krakenuniq_save_reads = false // added directly to module in profiling.nf + krakenuniq_save_readclassifications = false // added directly to module in profiling.nf + // Bracken run_bracken = false From 851016e497c01151c8a4bfb2ad93efc6f032f508 Mon Sep 17 00:00:00 2001 From: Mahwash Jamy Date: Mon, 31 Oct 2022 16:17:40 +0000 Subject: [PATCH 057/132] Update nextflow_scheme.json --- nextflow_schema.json | 72 +++++++++++++++++++++++++++++++++++++++----- 1 file changed, 64 insertions(+), 8 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 21e7e96..c7f4825 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,11 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "databases", "outdir"], + "required": [ + "input", + "databases", + "outdir" + ], "properties": { "input": { "type": "string", @@ -80,7 +84,10 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": ["fastp", "adapterremoval"], + "enum": [ + "fastp", + "adapterremoval" + ], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -133,7 +140,11 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], + "enum": [ + "bbduk", + "prinseqplusplus", + "fastp" + ], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -167,7 +178,10 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": ["entropy", "dust"], + "enum": [ + "entropy", + "dust" + ], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -341,7 +355,15 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "enum": [ + "blast", + "xml", + "txt", + "daa", + "sam", + "tsv", + "paf" + ], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -360,7 +382,14 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], + "enum": [ + "phylum", + "class", + "order", + "family", + "genus", + "species" + ], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -388,6 +417,23 @@ "fa_icon": "fas fa-save", "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." }, + "run_krakenuniq": { + "type": "string", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with KrakenUniq. Requires database to be present CSV file passed to --databases" + }, + "krakenuniq_save_reads": { + "type": "string", + "fa_icon": "fas fa-save", + "description": "Turn on saving of KrakenUniq-aligned reads", + "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - krakenuniq: `--classified-out` and `--unclassified-out`" + }, + "krakenuniq_save_readclassifications": { + "type": "string", + "fa_icon": "fas fa-save", + "description": "Turn on saving of KrakenUniq per-read taxonomic assignment file", + "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - krakenuniq: `--output`" + }, "run_bracken": { "type": "boolean", "description": "Post-process kraken2 reports with Bracken.", @@ -566,7 +612,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { @@ -723,7 +776,10 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": ["fastqc", "falco"], + "enum": [ + "fastqc", + "falco" + ], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "Specify the tool used for quality control of raw sequencing reads" } From aecf308840acc8800c766e2bb56896e56dd5f171 Mon Sep 17 00:00:00 2001 From: Mahwash Jamy Date: Mon, 31 Oct 2022 16:41:35 +0000 Subject: [PATCH 058/132] Update documentation for krakenuniq --- CITATIONS.md | 4 ++++ docs/usage.md | 17 ++++++++++++++++- 2 files changed, 20 insertions(+), 1 deletion(-) diff --git a/CITATIONS.md b/CITATIONS.md index 0415bf7..daf9022 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -36,6 +36,10 @@ > Wood, Derrick E., Jennifer Lu, and Ben Langmead. 2019. Improved Metagenomic Analysis with Kraken 2. Genome Biology 20 (1): 257. doi: 10.1186/s13059-019-1891-0. +- [KrakenUniq](https://doi.org/10.1186/s13059-018-1568-0) + + > Breitwieser, Florian P., Daniel N. Baker, and Steven L. Salzberg. 2018. KrakenUniq: confident and fast metagenomics classification using unique k-mer counts. Genome Biology 19 (1): 198. doi: 10.1186/s13059-018-1568-0 + - [Bracken](https://doi.org/10.7717/peerj-cs.104) > Lu, J., Breitwieser, F. P., Thielen, P., & Salzberg, S. L. (2017). Bracken: Estimating species abundance in metagenomics data. PeerJ Computer Science, 3, e104. doi: 10.7717/peerj-cs.104 diff --git a/docs/usage.md b/docs/usage.md index 0401540..11e077f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -74,7 +74,7 @@ The pipeline takes the locations and specific profiling parameters of the tool o > ⚠️ nf-core/taxprofiler does not provide any databases by default, nor does it currently generate them for you. This must be performed manually by the user. See below for more information of the expected database files. -An example database sheet can look as follows, where 4 tools are being used, and `malt` and `kraken2` will be used against two databases each. This is because specifying `bracken` implies first running `kraken2` on the same database. +An example database sheet can look as follows, where 5 tools are being used, and `malt` and `kraken2` will be used against two databases each. This is because specifying `bracken` implies first running `kraken2` on the same database. ```console tool,db_name,db_params,db_path @@ -82,6 +82,7 @@ malt,malt85,-id 85,///malt/testdb-malt/ malt,malt95,-id 90,///malt/testdb-malt.tar.gz bracken,db1,,///bracken/testdb-bracken.tar.gz kraken2,db2,--quick,///kraken2/testdb-kraken2.tar.gz +krakenuniq,db3,,///krakenuniq/testdb-krakenuniq.tar.gz centrifuge,db1,,///centrifuge/minigut_cf.tar.gz metaphlan3,db1,,///metaphlan3/metaphlan_database/ motus,db_mOTU,,///motus/motus_database/ @@ -125,6 +126,20 @@ Expected (uncompressed) database files for each tool are as follows: - `database100mers.kraken` - `database150mers.kmer_distrib` - `database150mers.kraken` +- **KrakenUniq** output of `krakenuniq-build` command(s) A directory containing: + - `opts.k2d` + - `hash.k2d` + - `taxo.k2d` + - `database.idx` + - `database.kdb.counts` + - `database50mers.kmer_distrib` + - `database75mers.kmer_distrib` + - `database100mers.kmer_distrib` + - `database150mers.kmer_distrib` + - `database200mers.kmer_distrib` + - `database300mers.kmer_distrib` + - `seqid2taxid.map` + - `taxDB` - **Centrifuge** output of `centrifuge-build`. A directory containing: - `..cf` - `..cf` From 3faea8aac574cd42ad3dd85f64f264521a274e18 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Tue, 1 Nov 2022 17:34:37 +0100 Subject: [PATCH 059/132] Fix porechop conflict --- modules.json | 4 ++-- .../nf-core/porechop/{ => porechop}/main.nf | 3 +-- .../nf-core/porechop/{ => porechop}/meta.yml | 2 +- subworkflows/local/longread_preprocessing.nf | 18 +++++++++--------- 4 files changed, 13 insertions(+), 14 deletions(-) rename modules/nf-core/porechop/{ => porechop}/main.nf (97%) rename modules/nf-core/porechop/{ => porechop}/meta.yml (98%) diff --git a/modules.json b/modules.json index be3d193..c0e0a3a 100644 --- a/modules.json +++ b/modules.json @@ -141,9 +141,9 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" }, - "porechop": { + "porechop/porechop": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + "git_sha": "2a4e85eb81875a572bb58133e37f84ba3cc484d7" }, "prinseqplusplus": { "branch": "master", diff --git a/modules/nf-core/porechop/main.nf b/modules/nf-core/porechop/porechop/main.nf similarity index 97% rename from modules/nf-core/porechop/main.nf rename to modules/nf-core/porechop/porechop/main.nf index 77050bc..f946417 100644 --- a/modules/nf-core/porechop/main.nf +++ b/modules/nf-core/porechop/porechop/main.nf @@ -1,4 +1,4 @@ -process PORECHOP { +process PORECHOP_PORECHOP { tag "$meta.id" label 'process_medium' @@ -28,7 +28,6 @@ process PORECHOP { $args \\ -o ${prefix}.fastq.gz \\ > ${prefix}.log - cat <<-END_VERSIONS > versions.yml "${task.process}": porechop: \$( porechop --version ) diff --git a/modules/nf-core/porechop/meta.yml b/modules/nf-core/porechop/porechop/meta.yml similarity index 98% rename from modules/nf-core/porechop/meta.yml rename to modules/nf-core/porechop/porechop/meta.yml index e526317..98b838f 100644 --- a/modules/nf-core/porechop/meta.yml +++ b/modules/nf-core/porechop/porechop/meta.yml @@ -1,4 +1,4 @@ -name: porechop +name: "porechop_porechop" description: Adapter removal and demultiplexing of Oxford Nanopore reads keywords: - adapter diff --git a/subworkflows/local/longread_preprocessing.nf b/subworkflows/local/longread_preprocessing.nf index 3da49ce..961417d 100644 --- a/subworkflows/local/longread_preprocessing.nf +++ b/subworkflows/local/longread_preprocessing.nf @@ -5,7 +5,7 @@ include { FASTQC as FASTQC_PROCESSED } from '../../modules/nf-core/fastqc/main' include { FALCO as FALCO_PROCESSED } from '../../modules/nf-core/falco/main' -include { PORECHOP } from '../../modules/nf-core/porechop/main' +include { PORECHOP_PORECHOP } from '../../modules/nf-core/porechop/porechop/main' include { FILTLONG } from '../../modules/nf-core/filtlong/main' workflow LONGREAD_PREPROCESSING { @@ -17,9 +17,9 @@ workflow LONGREAD_PREPROCESSING { ch_multiqc_files = Channel.empty() if ( !params.longread_qc_skipadaptertrim && params.longread_qc_skipqualityfilter) { - PORECHOP ( reads ) + PORECHOP_PORECHOP ( reads ) - ch_processed_reads = PORECHOP.out.reads + ch_processed_reads = PORECHOP_PORECHOP.out.reads .map { meta, reads -> def meta_new = meta.clone() @@ -27,8 +27,8 @@ workflow LONGREAD_PREPROCESSING { [ meta_new, reads ] } - ch_versions = ch_versions.mix(PORECHOP.out.versions.first()) - ch_multiqc_files = ch_multiqc_files.mix( PORECHOP.out.log ) + ch_versions = ch_versions.mix(PORECHOP_PORECHOP.out.versions.first()) + ch_multiqc_files = ch_multiqc_files.mix( PORECHOP_PORECHOP.out.log ) } else if ( params.longread_qc_skipadaptertrim && !params.longread_qc_skipqualityfilter) { @@ -37,8 +37,8 @@ workflow LONGREAD_PREPROCESSING { ch_multiqc_files = ch_multiqc_files.mix( FILTLONG.out.log ) } else { - PORECHOP ( reads ) - ch_clipped_reads = PORECHOP.out.reads + PORECHOP_PORECHOP ( reads ) + ch_clipped_reads = PORECHOP_PORECHOP.out.reads .map { meta, reads -> def meta_new = meta.clone() @@ -48,9 +48,9 @@ workflow LONGREAD_PREPROCESSING { ch_processed_reads = FILTLONG ( ch_clipped_reads.map{ meta, reads -> [meta, [], reads ]} ).reads - ch_versions = ch_versions.mix(PORECHOP.out.versions.first()) + ch_versions = ch_versions.mix(PORECHOP_PORECHOP.out.versions.first()) ch_versions = ch_versions.mix(FILTLONG.out.versions.first()) - ch_multiqc_files = ch_multiqc_files.mix( PORECHOP.out.log ) + ch_multiqc_files = ch_multiqc_files.mix( PORECHOP_PORECHOP.out.log ) ch_multiqc_files = ch_multiqc_files.mix( FILTLONG.out.log ) } From e4307ad8abfa123d749cd70ce37cff486e804590 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Tue, 1 Nov 2022 20:50:42 +0100 Subject: [PATCH 060/132] Change modules.config to include porechop_porechop --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 397e0c2..dad18a2 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -134,7 +134,7 @@ process { ] } - withName: PORECHOP { + withName: PORECHOP_PORECHOP { ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/porechop" }, From d487a1ff2fac2e0a4bf9b3aecc41792000256b6f Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Wed, 2 Nov 2022 12:39:34 +0100 Subject: [PATCH 061/132] Addd FASTP adadapter list --- conf/modules.config | 6 +++--- modules.json | 2 +- modules/nf-core/fastp/main.nf | 5 +++++ modules/nf-core/fastp/meta.yml | 4 ++++ nextflow_schema.json | 4 ++-- subworkflows/local/shortread_fastp.nf | 5 +++-- subworkflows/local/shortread_preprocessing.nf | 2 +- workflows/taxprofiler.nf | 11 +++++++---- 8 files changed, 26 insertions(+), 13 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 9a351cc..f47e587 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -62,7 +62,7 @@ process { ext.args = [ // trimming options params.shortread_qc_skipadaptertrim ? "--disable_adapter_trimming" : "", - params.shortread_qc_adapter1 ? "--adapter_sequence ${params.shortread_qc_adapter1}" : "", + params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter1 ? "--adapter_sequence ${params.shortread_qc_adapter1}" : "", // filtering options "--length_required ${params.shortread_qc_minlength}", (params.perform_shortread_complexityfilter && params.shortread_complexityfilter_tool == 'fastp') ? "--low_complexity_filter --complexity_threshold ${params.shortread_complexityfilter_fastp_threshold}" : '' @@ -82,8 +82,8 @@ process { params.shortread_qc_excludeunmerged ? '' : "--include_unmerged", // trimming options params.shortread_qc_skipadaptertrim ? "--disable_adapter_trimming" : "", - params.shortread_qc_adapter1 ? "--adapter_sequence ${params.shortread_qc_adapter1}" : "", - params.shortread_qc_adapter2 ? "--adapter_sequence_r2 ${params.shortread_qc_adapter2}" : "--detect_adapter_for_pe", + params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter1 ? "--adapter_sequence ${params.shortread_qc_adapter1}" : "", + params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter2 ? "--adapter_sequence_r2 ${params.shortread_qc_adapter2}" : "--detect_adapter_for_pe", // filtering options "--length_required ${params.shortread_qc_minlength}", params.perform_shortread_complexityfilter && params.shortread_complexityfilter_tool == 'fastp' ? "--low_complexity_filter --complexity_threshold ${params.shortread_complexityfilter_fastp_threshold}" : '' diff --git a/modules.json b/modules.json index ab32eec..3527a44 100644 --- a/modules.json +++ b/modules.json @@ -55,7 +55,7 @@ }, "fastp": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + "git_sha": "1e49f31e93c56a3832833eef90a02d3cde5a3f7e" }, "fastqc": { "branch": "master", diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf index 11ea4db..207258a 100644 --- a/modules/nf-core/fastp/main.nf +++ b/modules/nf-core/fastp/main.nf @@ -9,6 +9,7 @@ process FASTP { input: tuple val(meta), path(reads) + path adapter_fasta val save_trimmed_fail val save_merged @@ -27,6 +28,7 @@ process FASTP { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" + def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : "" def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : '' // Added soft-links to original fastqs for consistent naming in MultiQC // Use single ended for interleaved. Add --interleaved_in in config. @@ -40,6 +42,7 @@ process FASTP { --thread $task.cpus \\ --json ${prefix}.fastp.json \\ --html ${prefix}.fastp.html \\ + $adapter_list \\ $fail_fastq \\ $args \\ 2> ${prefix}.fastp.log \\ @@ -61,6 +64,7 @@ process FASTP { --thread $task.cpus \\ --json ${prefix}.fastp.json \\ --html ${prefix}.fastp.html \\ + $adapter_list \\ $fail_fastq \\ $args \\ 2> ${prefix}.fastp.log @@ -82,6 +86,7 @@ process FASTP { --out2 ${prefix}_2.fastp.fastq.gz \\ --json ${prefix}.fastp.json \\ --html ${prefix}.fastp.html \\ + $adapter_list \\ $fail_fastq \\ $merge_fastq \\ --thread $task.cpus \\ diff --git a/modules/nf-core/fastp/meta.yml b/modules/nf-core/fastp/meta.yml index 2368fde..6f6fad7 100644 --- a/modules/nf-core/fastp/meta.yml +++ b/modules/nf-core/fastp/meta.yml @@ -23,6 +23,10 @@ input: List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively. If you wish to run interleaved paired-end data, supply as single-end data but with `--interleaved_in` in your `modules.conf`'s `ext.args` for the module. + - adapter_fasta: + type: file + description: File in FASTA format containing possible adapters to remove. + pattern: "*.{fasta,fna,fas,fa}" - save_trimmed_fail: type: boolean description: Specify true to save files that failed to pass trimming thresholds ending in `*.fail.fastq.gz` diff --git a/nextflow_schema.json b/nextflow_schema.json index 04ba938..b96fdac 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -107,8 +107,8 @@ "shortread_qc_adapterlist": { "type": "string", "default": "None", - "fa_icon": "fas fa-list-ul", - "help_text": "This affects AdapterRemoval. It will replace default adapters or whatever is supplied in `--shortread_qc_adapter{1,2}`.\n\nThis allows you to mix libraries built with different adapters into one run.\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--adapter-list`." + "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", + "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possibly combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" }, "shortread_qc_mergepairs": { "type": "boolean", diff --git a/subworkflows/local/shortread_fastp.nf b/subworkflows/local/shortread_fastp.nf index d466041..cac5a27 100644 --- a/subworkflows/local/shortread_fastp.nf +++ b/subworkflows/local/shortread_fastp.nf @@ -8,6 +8,7 @@ include { FASTP as FASTP_PAIRED } from '../../modules/nf-core/fastp/main' workflow SHORTREAD_FASTP { take: reads // [[meta], [reads]] + adapterlist main: ch_versions = Channel.empty() @@ -19,9 +20,9 @@ workflow SHORTREAD_FASTP { paired: it[0]['single_end'] == false } - FASTP_SINGLE ( ch_input_for_fastp.single, false, false ) + FASTP_SINGLE ( ch_input_for_fastp.single, adapterlist, false, false ) // Last parameter here turns on merging of PE data - FASTP_PAIRED ( ch_input_for_fastp.paired, false, params.shortread_qc_mergepairs ) + FASTP_PAIRED ( ch_input_for_fastp.paired, adapterlist, false, params.shortread_qc_mergepairs ) if ( params.shortread_qc_mergepairs ) { ch_fastp_reads_prepped_pe = FASTP_PAIRED.out.reads_merged diff --git a/subworkflows/local/shortread_preprocessing.nf b/subworkflows/local/shortread_preprocessing.nf index d381d34..c823e3d 100644 --- a/subworkflows/local/shortread_preprocessing.nf +++ b/subworkflows/local/shortread_preprocessing.nf @@ -18,7 +18,7 @@ workflow SHORTREAD_PREPROCESSING { ch_multiqc_files = Channel.empty() if ( params.shortread_qc_tool == "fastp" ) { - ch_processed_reads = SHORTREAD_FASTP ( reads ).reads + ch_processed_reads = SHORTREAD_FASTP ( reads, adapterlist ).reads ch_versions = ch_versions.mix( SHORTREAD_FASTP.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_FASTP.out.mqc ) } else if ( params.shortread_qc_tool == "adapterremoval" ) { diff --git a/workflows/taxprofiler.nf b/workflows/taxprofiler.nf index e6ccc16..bf05d54 100644 --- a/workflows/taxprofiler.nf +++ b/workflows/taxprofiler.nf @@ -103,7 +103,12 @@ workflow TAXPROFILER { ch_versions = Channel.empty() ch_multiqc_logo= Channel.fromPath("$projectDir/docs/images/nf-core-taxprofiler_logo_custom_light.png") - ch_adapterlist_for_shortreadqc = params.shortread_qc_adapterlist ? file(params.shortread_qc_adapterlist) : [] + adapterlist = params.shortread_qc_adapterlist ? file(params.shortread_qc_adapterlist) : [] + + if ( params.shortread_qc_adapterlist ) { + if ( params.shortread_qc_tool == 'adapterremoval' && !(adapterlist.extension == 'txt') ) error "[nf-core/taxprofiler] ERROR: AdapterRemoval2 adapter list requires a `.txt` format and extension. Check input: --shortread_qc_adapterlist ${params.shortread_qc_adapterlist}" + if ( params.shortread_qc_tool == 'fastp' && !adapterlist.extension.matches(".*(fa|fasta|fna|fas)") ) error "[nf-core/taxprofiler] ERROR: fastp adapter list requires a `.fasta` format and extension (or fa, fas, fna). Check input: --shortread_qc_adapterlist ${params.shortread_qc_adapterlist}" + } /* SUBWORKFLOW: Read in samplesheet, validate and stage input files @@ -134,10 +139,8 @@ workflow TAXPROFILER { SUBWORKFLOW: PERFORM PREPROCESSING */ - ch_adapterlist_for_shortreadqc - if ( params.perform_shortread_qc ) { - ch_shortreads_preprocessed = SHORTREAD_PREPROCESSING ( INPUT_CHECK.out.fastq, ch_adapterlist_for_shortreadqc ).reads + ch_shortreads_preprocessed = SHORTREAD_PREPROCESSING ( INPUT_CHECK.out.fastq, adapterlist ).reads ch_versions = ch_versions.mix( SHORTREAD_PREPROCESSING.out.versions ) } else { ch_shortreads_preprocessed = INPUT_CHECK.out.fastq From 24dc2a2cecc77b5a5beb0a16042814374f985df5 Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Thu, 3 Nov 2022 10:03:07 +0100 Subject: [PATCH 062/132] Apply suggestions from code review Co-authored-by: Moritz E. Beber --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index b96fdac..7c7e8dc 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -108,7 +108,7 @@ "type": "string", "default": "None", "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", - "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possibly combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" + "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possible combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" }, "shortread_qc_mergepairs": { "type": "boolean", From 62f45c8be7b1f592843cb2a4ca0c214e51beaf81 Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Thu, 3 Nov 2022 10:03:27 +0100 Subject: [PATCH 063/132] Update nextflow.config --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 9bf7f83..d11abd9 100644 --- a/nextflow.config +++ b/nextflow.config @@ -69,7 +69,7 @@ params { shortread_qc_excludeunmerged = false shortread_qc_adapter1 = null shortread_qc_adapter2 = null - shortread_qc_adapterlist = null // TODO Add for FASTP + shortread_qc_adapterlist = null shortread_qc_minlength = 15 perform_longread_qc = false From 6459d8920fd53a8c03680f7b4b32bf9943f00123 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 3 Nov 2022 14:05:43 +0100 Subject: [PATCH 064/132] Linting! --- docs/usage.md | 12 ++---------- nextflow_schema.json | 6 +++--- 2 files changed, 5 insertions(+), 13 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 11e077f..a87102c 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -131,14 +131,6 @@ Expected (uncompressed) database files for each tool are as follows: - `hash.k2d` - `taxo.k2d` - `database.idx` - - `database.kdb.counts` - - `database50mers.kmer_distrib` - - `database75mers.kmer_distrib` - - `database100mers.kmer_distrib` - - `database150mers.kmer_distrib` - - `database200mers.kmer_distrib` - - `database300mers.kmer_distrib` - - `seqid2taxid.map` - `taxDB` - **Centrifuge** output of `centrifuge-build`. A directory containing: - `..cf` @@ -192,7 +184,7 @@ work # Directory containing the nextflow working files ### Sequencing quality control -nf-core taxprofiler offers [`falco`](https://github.com/smithlabcode/falco] as an alternative option to [`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/). +nf-core taxprofiler offers [`falco`][https://github.com/smithlabcode/falco] as an alternative option to [`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/). ### Preprocessing Steps @@ -457,7 +449,7 @@ NXF_OPTS='-Xms1g -Xmx4g' ## Troubleshooting and FAQs -### I get a warning during centrifuge_kreport process with exit status 255. +### I get a warning during centrifuge_kreport process with exit status 255 When a sample has insufficient hits for abundance estimation, the resulting `report.txt` file will be empty. diff --git a/nextflow_schema.json b/nextflow_schema.json index c7f4825..7f52764 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -418,18 +418,18 @@ "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." }, "run_krakenuniq": { - "type": "string", + "type": "boolean", "fa_icon": "fas fa-toggle-on", "description": "Turn on profiling with KrakenUniq. Requires database to be present CSV file passed to --databases" }, "krakenuniq_save_reads": { - "type": "string", + "type": "boolean", "fa_icon": "fas fa-save", "description": "Turn on saving of KrakenUniq-aligned reads", "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - krakenuniq: `--classified-out` and `--unclassified-out`" }, "krakenuniq_save_readclassifications": { - "type": "string", + "type": "boolean", "fa_icon": "fas fa-save", "description": "Turn on saving of KrakenUniq per-read taxonomic assignment file", "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - krakenuniq: `--output`" From b5e6639f9d01d2a56ac0e72715eb617b36c57812 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Thu, 3 Nov 2022 14:12:05 +0100 Subject: [PATCH 065/132] Apply review suggestions --- conf/modules.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 397e0c2..96bb658 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -238,7 +238,6 @@ process { publishDir = [ path: { "${params.outdir}/samtools/stats" }, mode: params.publish_dir_mode, - enabled: params.save_hostremoval_unmapped, pattern: '*stats' ] } From 0c445759e4a23f7f2d6e0fc172e0a9c075b742fe Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 3 Nov 2022 13:42:39 +0000 Subject: [PATCH 066/132] [automated] Fix linting with Prettier --- nextflow_schema.json | 55 +++++++------------------------------------- 1 file changed, 8 insertions(+), 47 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 7f52764..bb22fac 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,11 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "databases", - "outdir" - ], + "required": ["input", "databases", "outdir"], "properties": { "input": { "type": "string", @@ -84,10 +80,7 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], + "enum": ["fastp", "adapterremoval"], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -140,11 +133,7 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], + "enum": ["bbduk", "prinseqplusplus", "fastp"], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -178,10 +167,7 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": [ - "entropy", - "dust" - ], + "enum": ["entropy", "dust"], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -355,15 +341,7 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -382,14 +360,7 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], + "enum": ["phylum", "class", "order", "family", "genus", "species"], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -612,14 +583,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { @@ -776,10 +740,7 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": [ - "fastqc", - "falco" - ], + "enum": ["fastqc", "falco"], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "Specify the tool used for quality control of raw sequencing reads" } From 21a76c244943fb96dbe863c2f08b58335d0b00de Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 3 Nov 2022 14:56:26 +0100 Subject: [PATCH 067/132] Fix cardinality issue --- nextflow.config | 1 + nextflow_schema.json | 62 ++++++++------------------------- subworkflows/local/profiling.nf | 4 +-- 3 files changed, 18 insertions(+), 49 deletions(-) diff --git a/nextflow.config b/nextflow.config index 6223db0..262aa2d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -120,6 +120,7 @@ params { //krakenuniq run_krakenuniq = false + krakenuniq_ram_run_size = '16G' krakenuniq_save_reads = false // added directly to module in profiling.nf krakenuniq_save_readclassifications = false // added directly to module in profiling.nf diff --git a/nextflow_schema.json b/nextflow_schema.json index 7f52764..c40a0de 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,11 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "databases", - "outdir" - ], + "required": ["input", "databases", "outdir"], "properties": { "input": { "type": "string", @@ -84,10 +80,7 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], + "enum": ["fastp", "adapterremoval"], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -140,11 +133,7 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], + "enum": ["bbduk", "prinseqplusplus", "fastp"], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -178,10 +167,7 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": [ - "entropy", - "dust" - ], + "enum": ["entropy", "dust"], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -355,15 +341,7 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -382,14 +360,7 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], + "enum": ["phylum", "class", "order", "family", "genus", "species"], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -428,6 +399,13 @@ "description": "Turn on saving of KrakenUniq-aligned reads", "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - krakenuniq: `--classified-out` and `--unclassified-out`" }, + "krakenuniq_ram_run_size": { + "type": "string", + "default": "16G", + "description": "Specify how large to chunk database when loading into memory for KrakenUniq", + "fa_icon": "fas fa-database", + "help_text": "nf-core/taxprofiler utilises a 'low memory' option for KrakenUniq that can reduce the amount of RAM the process requires using the `--preloaded` option.\n\nA further extension to this option is that you can specify how large each chunk of the database should be that gets loaded into memory at any one time. You can specify the amount of RAM to chunk the database to with this parameter, and is particularly useful for people with limited computational resources.\n\nMore information about this parameter can be seen [here](https://github.com/fbreitwieser/krakenuniq/blob/master/README.md#new-release-v07).\n\n> Modifies KrakenUniq parameter: --preload\n\n> \n\n" + }, "krakenuniq_save_readclassifications": { "type": "boolean", "fa_icon": "fas fa-save", @@ -612,14 +590,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { @@ -776,10 +747,7 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": [ - "fastqc", - "falco" - ], + "enum": ["fastqc", "falco"], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "Specify the tool used for quality control of raw sequencing reads" } diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 73e0b73..849583e 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -274,8 +274,8 @@ workflow PROFILING { reads: [ it[0] + it[2], it[1] ] db: it[3] } - - KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads, ch_input_for_krakenuniq.db, params.krakenuniq_save_reads, params.krakenuniq_save_readclassification ) + // Hardcode to _always_ produce the report file (which is our basic otput, and goes into raw_profiles) + KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads, ch_input_for_krakenuniq.db, params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, true, params.krakenuniq_save_readclassification ) ch_multiqc_files = ch_multiqc_files.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.report ) ch_versions = ch_versions.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.versions.first() ) ch_raw_classifications = ch_raw_classifications.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.classified_assignment ) From c5e5696f84ca580a0a8c070f50223375d68d6386 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 3 Nov 2022 14:58:03 +0100 Subject: [PATCH 068/132] Fix parameter name --- nextflow.config | 2 +- nextflow_schema.json | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index 262aa2d..ce48db9 100644 --- a/nextflow.config +++ b/nextflow.config @@ -120,7 +120,7 @@ params { //krakenuniq run_krakenuniq = false - krakenuniq_ram_run_size = '16G' + krakenuniq_ram_chunk_size = '16G' krakenuniq_save_reads = false // added directly to module in profiling.nf krakenuniq_save_readclassifications = false // added directly to module in profiling.nf diff --git a/nextflow_schema.json b/nextflow_schema.json index c40a0de..69bd4f0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -399,7 +399,7 @@ "description": "Turn on saving of KrakenUniq-aligned reads", "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - krakenuniq: `--classified-out` and `--unclassified-out`" }, - "krakenuniq_ram_run_size": { + "krakenuniq_ram_chunk_size": { "type": "string", "default": "16G", "description": "Specify how large to chunk database when loading into memory for KrakenUniq", From 93f38b14a83dc98e4438724b7a5d8ed2bcf7e944 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 3 Nov 2022 15:01:00 +0100 Subject: [PATCH 069/132] Save report --- subworkflows/local/profiling.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 849583e..6930373 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -275,7 +275,8 @@ workflow PROFILING { db: it[3] } // Hardcode to _always_ produce the report file (which is our basic otput, and goes into raw_profiles) - KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads, ch_input_for_krakenuniq.db, params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, true, params.krakenuniq_save_readclassification ) + save_report = true + KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads, ch_input_for_krakenuniq.db, params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, save_report, params.krakenuniq_save_readclassification ) ch_multiqc_files = ch_multiqc_files.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.report ) ch_versions = ch_versions.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.versions.first() ) ch_raw_classifications = ch_raw_classifications.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.classified_assignment ) From 461482d22a2aa36e6f82f1b2ccc4c2eb12166c34 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 3 Nov 2022 15:02:22 +0100 Subject: [PATCH 070/132] Try again wit hthe corret param --- subworkflows/local/profiling.nf | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 6930373..2cdf1d1 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -274,9 +274,8 @@ workflow PROFILING { reads: [ it[0] + it[2], it[1] ] db: it[3] } - // Hardcode to _always_ produce the report file (which is our basic otput, and goes into raw_profiles) - save_report = true - KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads, ch_input_for_krakenuniq.db, params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, save_report, params.krakenuniq_save_readclassification ) + // Hardcode to _always_ produce the report file (which is our basic otput, and goes into) + KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads, ch_input_for_krakenuniq.db, params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, true, params.krakenuniq_save_readclassifications ) ch_multiqc_files = ch_multiqc_files.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.report ) ch_versions = ch_versions.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.versions.first() ) ch_raw_classifications = ch_raw_classifications.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.classified_assignment ) From 79dd3930bb0b714c99da55cb1c320c05295518f1 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Thu, 3 Nov 2022 16:52:01 +0100 Subject: [PATCH 071/132] Add samtools to README --- README.md | 11 ++++++----- 1 file changed, 6 insertions(+), 5 deletions(-) diff --git a/README.md b/README.md index 3f0a840..94ede1e 100644 --- a/README.md +++ b/README.md @@ -34,9 +34,10 @@ On release, automated continuous integration tests run the pipeline on a full-si 2. Performs optional read pre-processing - Adapter clipping and merging (short-read: [fastp](https://github.com/OpenGene/fastp), [AdapterRemoval2](https://github.com/MikkelSchubert/adapterremoval); long-read: [porechop](https://github.com/rrwick/Porechop)) - Low complexity and quality filtering (short-read: [bbduk](https://jgi.doe.gov/data-and-tools/software-tools/bbtools/), [PRINSEQ++](https://github.com/Adrian-Cantu/PRINSEQ-plus-plus); long-read: [Filtlong](https://github.com/rrwick/Filtlong)) - - Host read removal (short-read: [BowTie2](http://bowtie-bio.sourceforge.net/bowtie2/); long-read: [Minimap2](https://github.com/lh3/minimap2)) + - Host-read removal (short-read: [BowTie2](http://bowtie-bio.sourceforge.net/bowtie2/); long-read: [Minimap2](https://github.com/lh3/minimap2)) - Run merging -3. Performs taxonomic profiling using one or more of: +3. Supports statistics for host-read removal ([Samtools](http://www.htslib.org/)) +4. Performs taxonomic profiling using one or more of: - [Kraken2](https://ccb.jhu.edu/software/kraken2/) - [MetaPhlAn3](https://huttenhower.sph.harvard.edu/metaphlan/) - [MALT](https://uni-tuebingen.de/fakultaeten/mathematisch-naturwissenschaftliche-fakultaet/fachbereiche/informatik/lehrstuehle/algorithms-in-bioinformatics/software/malt/) @@ -45,10 +46,10 @@ On release, automated continuous integration tests run the pipeline on a full-si - [Kaiju](https://kaiju.binf.ku.dk/) - [mOTUs](https://motu-tool.org/) - [KrakenUniq](https://github.com/fbreitwieser/krakenuniq) -4. Perform optional post-processing with: +5. Perform optional post-processing with: - [bracken](https://ccb.jhu.edu/software/bracken/) -5. Standardises output tables -6. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +6. Standardises output tables +7. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) 7. Plotting Kraken2, Centrifuge, Kaiju and MALT results ([`Krona`](https://hpc.nih.gov/apps/kronatools.html)) ## Quick Start From 696edfb866a1d196241ea819e7207edd8cd067e8 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Fri, 4 Nov 2022 12:05:23 +0100 Subject: [PATCH 072/132] Apply prettier --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 94ede1e..5a8884e 100644 --- a/README.md +++ b/README.md @@ -50,7 +50,7 @@ On release, automated continuous integration tests run the pipeline on a full-si - [bracken](https://ccb.jhu.edu/software/bracken/) 6. Standardises output tables 7. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) -7. Plotting Kraken2, Centrifuge, Kaiju and MALT results ([`Krona`](https://hpc.nih.gov/apps/kronatools.html)) +8. 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211.66668" version="1.1" id="svg5" - inkscape:version="1.2 (1:1.2.1+202207142221+cd75a1ee6d)" + inkscape:version="1.2.1 (1:1.2.1+202210291243+9c6d41e410)" sodipodi:docname="taxprofiler_tube.svg" xml:space="preserve" inkscape:export-filename="taxprofiler_tube.png" @@ -26,7 +26,7 @@ inkscape:pageopacity="0.0" inkscape:pagecheckerboard="true" inkscape:document-units="mm" - showgrid="true" + showgrid="false" inkscape:snap-bbox="true" inkscape:bbox-nodes="true" inkscape:snap-bbox-edge-midpoints="false" @@ -37,14 +37,14 @@ fit-margin-right="0" fit-margin-bottom="0" inkscape:zoom="0.71542514" - inkscape:cx="808.61011" + inkscape:cx="364.11916" inkscape:cy="440.29764" inkscape:window-width="1920" - inkscape:window-height="1043" + inkscape:window-height="1163" inkscape:window-x="0" inkscape:window-y="0" inkscape:window-maximized="1" - inkscape:current-layer="g27886" + inkscape:current-layer="g3070" inkscape:showpageshadow="2" inkscape:deskcolor="#d1d1d1">FILTERING)(HOST REMOVAL)(HOST 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zaD~*`+`r+)KUYH@`|_0JK|Xx+T+;MPj=_FQQs(&Lv-kwWmOGTG8MK;+MYSI_;8qxL z+5}(2!fvPN)5VL#`$P$WTMnvX_1{IjN0w&{uKxirAQ?IMp$O*^aDvhWeYDoOLiDyw zeizf29)yJ*iG5WfwRz4gtD!Fysh+l(%RBzA#v|YNrq52KLmQlul0d-qbg)fs^BJ8V zApKIy?QS52N)(MgO@BJVCoZvdtIDZsGBldE(SO12`)VZuLq;{TT#iPZI_bDJ^WE2n~SI;wH3i+P+b~(5nZb zP?xntsBnA&8u-MR68GC!WyKDQN&N&E!MMZY+!}B*b;9G6A2{ezC`s2Kv7hQafTB(m z$@!mmM!q_DcA`6}9LZKhu3bmeRa!Fxf-6DMX4>wQRi@N}*Jr zns|3dmGae|1bLr;#E4m%4>s5PKcDSy-ifb?g}H4-gkx_+13Ix9H4 zYdI`a{_#8de0SA-c)HQ5Yd-zwulxHUTJTfs&l7QU%eex{Yw5}(CR|_~=(TKsp*sq$ zC#O2)7=G5lJH_5o?{+x;qzeAxvf>-3vHjN?8w$00yO&Pr<>JuG19=)xMJod?vk0i9 zCXy)aVVhK}r#$zw`T{{GKsbhgKOvN~xmw8}rRw=unNhY+?mxdXac zK`}R2yjZdX1VLzQFWx1Xubsq~o_x@N0f2L=ZtTn_&~mBzzLJ(k3CFAGPisH;=UQGRD=> zt5d*f$$yxVqLar!#Z5>*Oe)zB{v638bSCp2d_$=cLKu+BSX0PjJ$Z>J@!X!p zg%;8lPOT)zsWz9<+1Mu$zEwhtS~0gmDD?svBzCUB^5x$Uf^v>ssIGuVOet!W$=b<6 zaP_2?N6}0;QpC#vo;G}DNA=qmUUKryU3uoCXV~tYt2oXxe+zTo%ggbKbIU7Zo|Lh5&7Hs|9hDK8x9xWE6&+v&YL}LBM**+ QkoT&kuBVo^$KvAu15DJZxBvhE diff --git a/docs/images/taxprofiler_tube.svg b/docs/images/taxprofiler_tube.svg index 87c66c8..c4d2403 100644 --- a/docs/images/taxprofiler_tube.svg +++ b/docs/images/taxprofiler_tube.svg @@ -36,15 +36,15 @@ fit-margin-left="0" fit-margin-right="0" fit-margin-bottom="0" - inkscape:zoom="0.71542514" - inkscape:cx="364.11916" - inkscape:cy="440.29764" + inkscape:zoom="1.0117639" + inkscape:cx="596.48301" + inkscape:cy="463.05268" inkscape:window-width="1920" - inkscape:window-height="1163" + inkscape:window-height="1016" inkscape:window-x="0" inkscape:window-y="0" inkscape:window-maximized="1" - inkscape:current-layer="g3070" + inkscape:current-layer="g27886" inkscape:showpageshadow="2" inkscape:deskcolor="#d1d1d1">FastQCFastQCfalcoFastQCfalcoFastQCFastQCfalcoFastQCFastQCfalco Date: Tue, 22 Nov 2022 10:13:10 +0100 Subject: [PATCH 075/132] chore: let all tests run to completion --- .github/workflows/ci.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 862e072..a2a1662 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -18,6 +18,7 @@ jobs: if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/taxprofiler') }}" runs-on: ubuntu-latest strategy: + fail-fast: false matrix: NXF_VER: - "21.10.3" From 0f298dca4351cd4e57953ed025084c60db2e80f4 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 12:13:08 +0100 Subject: [PATCH 076/132] group krakenuniq reads by pairs or single end --- subworkflows/local/profiling.nf | 10 ++++++++++ 1 file changed, 10 insertions(+) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 2cdf1d1..8c09af6 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -269,6 +269,16 @@ workflow PROFILING { if ( params.run_krakenuniq ) { ch_input_for_krakenuniq = ch_input_for_profiling.krakenuniq + .map { + meta, reads, db_meta, db -> + def meta_new = [:] + meta_new['tool'] = meta['tool'] + meta_new['single_end'] = meta['single_end'] + + [meta_new, reads, db_meta, db] + } + .groupTuple(by: [0,2,3]) + .dump(tag: "krakenuniq_premultimap") .multiMap { it -> reads: [ it[0] + it[2], it[1] ] From db334c51852d2b9a8b333df7f018405633500195 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 12:17:08 +0100 Subject: [PATCH 077/132] Fix missing info --- subworkflows/local/profiling.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 8c09af6..51a38e3 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -272,7 +272,6 @@ workflow PROFILING { .map { meta, reads, db_meta, db -> def meta_new = [:] - meta_new['tool'] = meta['tool'] meta_new['single_end'] = meta['single_end'] [meta_new, reads, db_meta, db] From b8937748d311d863aafeed02939388c3a2853c05 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 12:18:52 +0100 Subject: [PATCH 078/132] Fix meta.id isssue --- subworkflows/local/profiling.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 51a38e3..80e0850 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -272,6 +272,7 @@ workflow PROFILING { .map { meta, reads, db_meta, db -> def meta_new = [:] + meta['id'] = db['db_name'] meta_new['single_end'] = meta['single_end'] [meta_new, reads, db_meta, db] From 52fc00802bbde66722232b44076d5fdda7342d92 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 12:19:56 +0100 Subject: [PATCH 079/132] Correct meta name for db meta --- subworkflows/local/profiling.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 80e0850..4fc490d 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -272,7 +272,7 @@ workflow PROFILING { .map { meta, reads, db_meta, db -> def meta_new = [:] - meta['id'] = db['db_name'] + meta['id'] = db_meta['db_name'] meta_new['single_end'] = meta['single_end'] [meta_new, reads, db_meta, db] From 5caebcc6ab38d2c6d557da77317eaba9965a5f6d Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 12:21:59 +0100 Subject: [PATCH 080/132] Append to correct db --- subworkflows/local/profiling.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 4fc490d..e43a915 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -272,7 +272,7 @@ workflow PROFILING { .map { meta, reads, db_meta, db -> def meta_new = [:] - meta['id'] = db_meta['db_name'] + meta_new['id'] = db_meta['db_name'] meta_new['single_end'] = meta['single_end'] [meta_new, reads, db_meta, db] From 557f2a4d966ffd93137780f002a66c714d412865 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 12:26:46 +0100 Subject: [PATCH 081/132] Extra dump --- subworkflows/local/profiling.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index e43a915..f904cc1 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -285,7 +285,7 @@ workflow PROFILING { db: it[3] } // Hardcode to _always_ produce the report file (which is our basic otput, and goes into) - KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads, ch_input_for_krakenuniq.db, params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, true, params.krakenuniq_save_readclassifications ) + KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads.dump(tag: "krakenuniq_input"), ch_input_for_krakenuniq.db, params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, true, params.krakenuniq_save_readclassifications ) ch_multiqc_files = ch_multiqc_files.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.report ) ch_versions = ch_versions.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.versions.first() ) ch_raw_classifications = ch_raw_classifications.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.classified_assignment ) From 3102e4005257fe7ff17c24b319774d0805431897 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 12:46:26 +0100 Subject: [PATCH 082/132] dump teh db --- subworkflows/local/profiling.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index f904cc1..a46a773 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -285,7 +285,7 @@ workflow PROFILING { db: it[3] } // Hardcode to _always_ produce the report file (which is our basic otput, and goes into) - KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads.dump(tag: "krakenuniq_input"), ch_input_for_krakenuniq.db, params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, true, params.krakenuniq_save_readclassifications ) + KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads.dump(tag: "krakenuniq_input"), ch_input_for_krakenuniq.db.dump(tag: "krakenuniq_db"), params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, true, params.krakenuniq_save_readclassifications ) ch_multiqc_files = ch_multiqc_files.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.report ) ch_versions = ch_versions.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.versions.first() ) ch_raw_classifications = ch_raw_classifications.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.classified_assignment ) From 21759807f76bed0e34730c5b5cb90eed119ff9c2 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 12:48:54 +0100 Subject: [PATCH 083/132] Does flatten work? --- subworkflows/local/profiling.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index a46a773..291d476 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -281,7 +281,7 @@ workflow PROFILING { .dump(tag: "krakenuniq_premultimap") .multiMap { it -> - reads: [ it[0] + it[2], it[1] ] + reads: [ it[0] + it[2], it[1].flatten() ] db: it[3] } // Hardcode to _always_ produce the report file (which is our basic otput, and goes into) From ebfc84e235b6e1a79cdcfb6787652773393ed58a Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 13:26:54 +0100 Subject: [PATCH 084/132] Does the grouping work if we have meta? --- modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf | 1 - subworkflows/local/profiling.nf | 1 - 2 files changed, 2 deletions(-) diff --git a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf index a355c13..85d35bd 100644 --- a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf +++ b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf @@ -1,5 +1,4 @@ process KRAKENUNIQ_PRELOADEDKRAKENUNIQ { - tag "$meta.id" label 'process_high' conda (params.enable_conda ? "bioconda::krakenuniq=1.0.0" : null) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 291d476..6e45648 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -272,7 +272,6 @@ workflow PROFILING { .map { meta, reads, db_meta, db -> def meta_new = [:] - meta_new['id'] = db_meta['db_name'] meta_new['single_end'] = meta['single_end'] [meta_new, reads, db_meta, db] From e8de006ed65bfddc80d56e977880e40c3f70600c Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 13:27:49 +0100 Subject: [PATCH 085/132] With a join --- modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf index 85d35bd..b0885c0 100644 --- a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf +++ b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf @@ -87,7 +87,7 @@ process KRAKENUNIQ_PRELOADEDKRAKENUNIQ { --preload $ram_chunk_size \\ --threads $task.cpus" - for fastq in ${fastqs.join(' ')}; do \\ + for fastq in "${fastqs.join('\" \"')}"; do \\ PREFIX=\$(echo \$fastq) echo "krakenuniq \\ --db $db \\ From cacd7d3128f1833cd19ec5627078e0d3570d219c Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 13:33:13 +0100 Subject: [PATCH 086/132] Not just in stub --- modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf index b0885c0..362d3a9 100644 --- a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf +++ b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf @@ -45,7 +45,7 @@ process KRAKENUNIQ_PRELOADEDKRAKENUNIQ { --preload $ram_chunk_size \\ --threads $task.cpus - for fastq in ${fastqs.join(' ')}; do \\ + for fastq in "${fastqs.join('\" \"')}"; do \\ PREFIX=\$(echo \$fastq) krakenuniq \\ --db $db \\ From 06659af777ae59960f161222b4a01d85be64f24c Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 13:36:40 +0100 Subject: [PATCH 087/132] Remove flatten --- subworkflows/local/profiling.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 6e45648..18b40b4 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -280,7 +280,7 @@ workflow PROFILING { .dump(tag: "krakenuniq_premultimap") .multiMap { it -> - reads: [ it[0] + it[2], it[1].flatten() ] + reads: [ it[0] + it[2], it[1] ] db: it[3] } // Hardcode to _always_ produce the report file (which is our basic otput, and goes into) From a383c9ae4099d0b4bfed75d77e830faabe26e04a Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 22 Nov 2022 13:39:21 +0100 Subject: [PATCH 088/132] Remove the join --- modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf index 362d3a9..dc9b5a4 100644 --- a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf +++ b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf @@ -45,7 +45,8 @@ process KRAKENUNIQ_PRELOADEDKRAKENUNIQ { --preload $ram_chunk_size \\ --threads $task.cpus - for fastq in "${fastqs.join('\" \"')}"; do \\ +# for fastq in "${fastqs.join('\" \"')}"; do \\ + for fastq in ${fastqs}; do \\ PREFIX=\$(echo \$fastq) krakenuniq \\ --db $db \\ From 30af26a8b361f3d0e79e24fda84c28d9cd265df9 Mon Sep 17 00:00:00 2001 From: sofstam Date: Tue, 22 Nov 2022 16:50:50 +0100 Subject: [PATCH 089/132] Include the mapped reads in samtools stats --- subworkflows/local/longread_hostremoval.nf | 4 ++-- subworkflows/local/shortread_hostremoval.nf | 6 +++--- 2 files changed, 5 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/longread_hostremoval.nf b/subworkflows/local/longread_hostremoval.nf index 5f3f746..b6a9419 100644 --- a/subworkflows/local/longread_hostremoval.nf +++ b/subworkflows/local/longread_hostremoval.nf @@ -44,8 +44,8 @@ workflow LONGREAD_HOSTREMOVAL { SAMTOOLS_INDEX ( SAMTOOLS_VIEW.out.bam ) ch_versions = ch_versions.mix( SAMTOOLS_INDEX.out.versions.first() ) - bam_bai = SAMTOOLS_VIEW.out.bam - .join(SAMTOOLS_INDEX.out.bai, remainder: true) + bam_bai = MINIMAP2_ALIGN.out.bam + .join(SAMTOOLS_INDEX.out.bai,by:[0], remainder: true) SAMTOOLS_STATS ( bam_bai, reference ) ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) diff --git a/subworkflows/local/shortread_hostremoval.nf b/subworkflows/local/shortread_hostremoval.nf index 426442b..e0254c6 100644 --- a/subworkflows/local/shortread_hostremoval.nf +++ b/subworkflows/local/shortread_hostremoval.nf @@ -25,7 +25,7 @@ workflow SHORTREAD_HOSTREMOVAL { ch_bowtie2_index = index.first() } - BOWTIE2_ALIGN ( reads, ch_bowtie2_index, true, false ) + BOWTIE2_ALIGN ( reads, ch_bowtie2_index, true, true) ch_versions = ch_versions.mix( BOWTIE2_ALIGN.out.versions.first() ) ch_multiqc_files = ch_multiqc_files.mix( BOWTIE2_ALIGN.out.log ) @@ -41,8 +41,8 @@ workflow SHORTREAD_HOSTREMOVAL { SAMTOOLS_INDEX ( SAMTOOLS_VIEW.out.bam ) ch_versions = ch_versions.mix( SAMTOOLS_INDEX.out.versions.first() ) - bam_bai = SAMTOOLS_VIEW.out.bam - .join(SAMTOOLS_INDEX.out.bai, remainder: true) + bam_bai = BOWTIE2_ALIGN.out.bam + .join(SAMTOOLS_INDEX.out.bai, by:[0], remainder: true) SAMTOOLS_STATS ( bam_bai, reference ) ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) From 481f90589cb0941a6d3fad49cd1c0bea288a4676 Mon Sep 17 00:00:00 2001 From: sofstam Date: Wed, 23 Nov 2022 09:08:22 +0100 Subject: [PATCH 090/132] Apply review suggestions --- subworkflows/local/longread_hostremoval.nf | 2 +- subworkflows/local/shortread_hostremoval.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/longread_hostremoval.nf b/subworkflows/local/longread_hostremoval.nf index b6a9419..3f9fdef 100644 --- a/subworkflows/local/longread_hostremoval.nf +++ b/subworkflows/local/longread_hostremoval.nf @@ -45,7 +45,7 @@ workflow LONGREAD_HOSTREMOVAL { ch_versions = ch_versions.mix( SAMTOOLS_INDEX.out.versions.first() ) bam_bai = MINIMAP2_ALIGN.out.bam - .join(SAMTOOLS_INDEX.out.bai,by:[0], remainder: true) + .join(SAMTOOLS_INDEX.out.bai, remainder: true) SAMTOOLS_STATS ( bam_bai, reference ) ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) diff --git a/subworkflows/local/shortread_hostremoval.nf b/subworkflows/local/shortread_hostremoval.nf index e0254c6..04c8556 100644 --- a/subworkflows/local/shortread_hostremoval.nf +++ b/subworkflows/local/shortread_hostremoval.nf @@ -42,7 +42,7 @@ workflow SHORTREAD_HOSTREMOVAL { ch_versions = ch_versions.mix( SAMTOOLS_INDEX.out.versions.first() ) bam_bai = BOWTIE2_ALIGN.out.bam - .join(SAMTOOLS_INDEX.out.bai, by:[0], remainder: true) + .join(SAMTOOLS_INDEX.out.bai, remainder: true) SAMTOOLS_STATS ( bam_bai, reference ) ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) From 9feb6c2db6f4d6e16d4a75ceb0e4f901fd5e5f20 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 24 Nov 2022 14:44:04 +0100 Subject: [PATCH 091/132] Update MALT --- conf/modules.config | 2 +- modules.json | 2 +- modules/nf-core/malt/run/main.nf | 8 +++----- subworkflows/local/profiling.nf | 2 +- 4 files changed, 6 insertions(+), 8 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index f9d51d8..ea569b6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -281,7 +281,7 @@ process { } withName: MALT_RUN { - ext.args = { "${meta.db_params}" } + ext.args = { "${meta.db_params} -m ${params.malt_mode}" } // one run with multiple samples, so fix ID to just db name to ensure clean log name ext.prefix = { "${meta.db_name}" } publishDir = [ diff --git a/modules.json b/modules.json index 62f6539..fabd8db 100644 --- a/modules.json +++ b/modules.json @@ -107,7 +107,7 @@ }, "malt/run": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + "git_sha": "6d9712f03ec2de8264a50ee4541a617e1e063b51" }, "megan/rma2info": { "branch": "master", diff --git a/modules/nf-core/malt/run/main.nf b/modules/nf-core/malt/run/main.nf index 2b91d90..2e75b4c 100644 --- a/modules/nf-core/malt/run/main.nf +++ b/modules/nf-core/malt/run/main.nf @@ -2,14 +2,13 @@ process MALT_RUN { tag "$meta.id" label 'process_high' - conda (params.enable_conda ? "bioconda::malt=0.41" : null) + conda (params.enable_conda ? "bioconda::malt=0.61" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/malt:0.41--1' : - 'quay.io/biocontainers/malt:0.41--1' }" + 'https://depot.galaxyproject.org/singularity/malt:0.61--hdfd78af_0' : + 'quay.io/biocontainers/malt:0.61--hdfd78af_0' }" input: tuple val(meta), path(fastqs) - val mode path index output: @@ -38,7 +37,6 @@ process MALT_RUN { -o . \\ $args \\ --inFile ${fastqs.join(' ')} \\ - -m $mode \\ --index $index/ |&tee ${prefix}-malt-run.log cat <<-END_VERSIONS > versions.yml diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index b86c165..6cac3e1 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -95,7 +95,7 @@ workflow PROFILING { db: it[2] } - MALT_RUN ( ch_input_for_malt.reads, params.malt_mode, ch_input_for_malt.db ) + MALT_RUN ( ch_input_for_malt.reads, ch_input_for_malt.db ) ch_maltrun_for_megan = MALT_RUN.out.rma6 .transpose() From 131b39aa8b3ecfae3d18f781f7440929243ab3a6 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 24 Nov 2022 15:49:06 +0100 Subject: [PATCH 092/132] Fix linting --- modules/nf-core/malt/run/meta.yml | 4 ---- 1 file changed, 4 deletions(-) diff --git a/modules/nf-core/malt/run/meta.yml b/modules/nf-core/malt/run/meta.yml index 66f2d7a..8fa1958 100644 --- a/modules/nf-core/malt/run/meta.yml +++ b/modules/nf-core/malt/run/meta.yml @@ -28,10 +28,6 @@ input: type: file description: Input FASTQ files pattern: "*.{fastq.gz,fq.gz}" - - mode: - type: string - description: Program mode - pattern: "Unknown|BlastN|BlastP|BlastX|Classifier" - index: type: directory description: Index/database directory from malt-build From a20b3e3c58a54274f908afdb231739f51eb3da4e Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 24 Nov 2022 15:51:12 +0100 Subject: [PATCH 093/132] Try to reduce MALT memory --- conf/test.config | 1 + conf/test_nopreprocessing.config | 1 + conf/test_nothing.config | 1 + conf/test_pep.config | 1 + lib/WorkflowMain.groovy | 15 ++++++++------- 5 files changed, 12 insertions(+), 7 deletions(-) diff --git a/conf/test.config b/conf/test.config index 777d9bf..03b8307 100644 --- a/conf/test.config +++ b/conf/test.config @@ -51,6 +51,7 @@ params { process { withName: MALT_RUN { maxForks = 1 + ext.args = "-m ${params.malt_mode} -J-Xmx8G" } withName: MEGAN_RMA2INFO_TSV { maxForks = 1 diff --git a/conf/test_nopreprocessing.config b/conf/test_nopreprocessing.config index 357f76f..c87cf6c 100644 --- a/conf/test_nopreprocessing.config +++ b/conf/test_nopreprocessing.config @@ -45,5 +45,6 @@ params { process { withName: MALT_RUN { maxForks = 1 + ext.args = "-m ${params.malt_mode} -J-Xmx8G" } } diff --git a/conf/test_nothing.config b/conf/test_nothing.config index df09613..0a969a6 100644 --- a/conf/test_nothing.config +++ b/conf/test_nothing.config @@ -44,5 +44,6 @@ params { process { withName: MALT_RUN { maxForks = 1 + ext.args = "-m ${params.malt_mode} -J-Xmx8G" } } diff --git a/conf/test_pep.config b/conf/test_pep.config index 762ebb3..3cd6dd0 100644 --- a/conf/test_pep.config +++ b/conf/test_pep.config @@ -37,6 +37,7 @@ params { process { withName: MALT_RUN { maxForks = 1 + ext.args = "-m ${params.malt_mode} -J-Xmx8G" } withName: MEGAN_RMA2INFO { maxForks = 1 diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 7883d70..02d4347 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -12,9 +12,9 @@ class WorkflowMain { // TODO nf-core: Add Zenodo DOI for pipeline after first release //"* The pipeline\n" + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + + '* The nf-core framework\n' + + ' https://doi.org/10.1038/s41587-020-0439-x\n\n' + + '* Software dependencies\n' + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" } @@ -53,15 +53,15 @@ class WorkflowMain { System.exit(0) } + // Print parameter summary log to screen + + log.info paramsSummaryLog(workflow, params, log) + // Validate workflow parameters via the JSON schema if (params.validate_params) { NfcoreSchema.validateParameters(workflow, params, log) } - // Print parameter summary log to screen - - log.info paramsSummaryLog(workflow, params, log) - // Check that a -profile or Nextflow config has been provided to run the pipeline NfcoreTemplate.checkConfigProvided(workflow, log) @@ -90,4 +90,5 @@ class WorkflowMain { } return null } + } From 17b1e3df1c230f1767deb7cb7f3e1bdf5db09556 Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Thu, 24 Nov 2022 16:53:39 +0100 Subject: [PATCH 094/132] Update test.config --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 03b8307..b3ad7bd 100644 --- a/conf/test.config +++ b/conf/test.config @@ -51,7 +51,7 @@ params { process { withName: MALT_RUN { maxForks = 1 - ext.args = "-m ${params.malt_mode} -J-Xmx8G" + ext.args = "-m ${params.malt_mode} -J-Xmx12G" } withName: MEGAN_RMA2INFO_TSV { maxForks = 1 From f114cc41220c9e48bc3a2f6f6c2e72e5ecade8a8 Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Thu, 24 Nov 2022 16:55:19 +0100 Subject: [PATCH 095/132] Apply suggestions from code review --- conf/test.config | 2 +- conf/test_nopreprocessing.config | 2 +- conf/test_nothing.config | 2 +- conf/test_pep.config | 2 +- 4 files changed, 4 insertions(+), 4 deletions(-) diff --git a/conf/test.config b/conf/test.config index b3ad7bd..e79bef4 100644 --- a/conf/test.config +++ b/conf/test.config @@ -51,7 +51,7 @@ params { process { withName: MALT_RUN { maxForks = 1 - ext.args = "-m ${params.malt_mode} -J-Xmx12G" + ext.args = { "-m ${params.malt_mode} -J-Xmx12G" } } withName: MEGAN_RMA2INFO_TSV { maxForks = 1 diff --git a/conf/test_nopreprocessing.config b/conf/test_nopreprocessing.config index c87cf6c..eae6144 100644 --- a/conf/test_nopreprocessing.config +++ b/conf/test_nopreprocessing.config @@ -45,6 +45,6 @@ params { process { withName: MALT_RUN { maxForks = 1 - ext.args = "-m ${params.malt_mode} -J-Xmx8G" + ext.args = { "-m ${params.malt_mode} -J-Xmx12G" } } } diff --git a/conf/test_nothing.config b/conf/test_nothing.config index 0a969a6..ca75380 100644 --- a/conf/test_nothing.config +++ b/conf/test_nothing.config @@ -44,6 +44,6 @@ params { process { withName: MALT_RUN { maxForks = 1 - ext.args = "-m ${params.malt_mode} -J-Xmx8G" + ext.args = { "-m ${params.malt_mode} -J-Xmx12G" } } } diff --git a/conf/test_pep.config b/conf/test_pep.config index 3cd6dd0..6ce788d 100644 --- a/conf/test_pep.config +++ b/conf/test_pep.config @@ -37,7 +37,7 @@ params { process { withName: MALT_RUN { maxForks = 1 - ext.args = "-m ${params.malt_mode} -J-Xmx8G" + ext.args = { "-m ${params.malt_mode} -J-Xmx12G" } } withName: MEGAN_RMA2INFO { maxForks = 1 From f13185d98bcea2d45ccd220d189c6035e347f101 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 29 Nov 2022 10:13:46 +0100 Subject: [PATCH 096/132] Fix schema location ofr fastqc/falco option --- nextflow_schema.json | 72 +++++++++++++++++++++++++++++++++----------- 1 file changed, 55 insertions(+), 17 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index a66b0f1..fd0a031 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,11 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "databases", "outdir"], + "required": [ + "input", + "databases", + "outdir" + ], "properties": { "input": { "type": "string", @@ -56,6 +60,17 @@ "description": "Common options across both long and short read preprocessing QC steps", "default": "", "properties": { + "preprocessing_qc_tool": { + "type": "string", + "default": "fastqc", + "enum": [ + "fastqc", + "falco" + ], + "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", + "description": "Specify the tool used for quality control of raw sequencing reads", + "fa_icon": "fas fa-tools" + }, "save_preprocessed_reads": { "type": "boolean", "fa_icon": "fas fa-save", @@ -80,7 +95,10 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": ["fastp", "adapterremoval"], + "enum": [ + "fastp", + "adapterremoval" + ], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -139,7 +157,11 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], + "enum": [ + "bbduk", + "prinseqplusplus", + "fastp" + ], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -173,7 +195,10 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": ["entropy", "dust"], + "enum": [ + "entropy", + "dust" + ], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -347,7 +372,15 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "enum": [ + "blast", + "xml", + "txt", + "daa", + "sam", + "tsv", + "paf" + ], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -366,7 +399,14 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], + "enum": [ + "phylum", + "class", + "order", + "family", + "genus", + "species" + ], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -572,7 +612,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { @@ -724,14 +771,5 @@ { "$ref": "#/definitions/reference_genome_options" } - ], - "properties": { - "preprocessing_qc_tool": { - "type": "string", - "default": "fastqc", - "enum": ["fastqc", "falco"], - "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", - "description": "Specify the tool used for quality control of raw sequencing reads" - } - } + ] } From ddcf38de22011a59446e5343c7bbcae430a03529 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Tue, 29 Nov 2022 10:14:20 +0100 Subject: [PATCH 097/132] refactor: make mappings explicit --- subworkflows/local/profiling.nf | 11 ++++------- 1 file changed, 4 insertions(+), 7 deletions(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 18b40b4..4ca2c4c 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -271,17 +271,14 @@ workflow PROFILING { ch_input_for_krakenuniq = ch_input_for_profiling.krakenuniq .map { meta, reads, db_meta, db -> - def meta_new = [:] - meta_new['single_end'] = meta['single_end'] - - [meta_new, reads, db_meta, db] + [[single_end: meta.single_end], reads, db_meta, db] } .groupTuple(by: [0,2,3]) .dump(tag: "krakenuniq_premultimap") .multiMap { - it -> - reads: [ it[0] + it[2], it[1] ] - db: it[3] + single_meta, reads, db_meta, db -> + reads: [ single_meta + db_meta, reads.flatten() ] + db: db } // Hardcode to _always_ produce the report file (which is our basic otput, and goes into) KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads.dump(tag: "krakenuniq_input"), ch_input_for_krakenuniq.db.dump(tag: "krakenuniq_db"), params.krakenuniq_ram_chunk_size, params.krakenuniq_save_reads, true, params.krakenuniq_save_readclassifications ) From d6c0ebb4c6256375bfc152fa968c738d188958a0 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 29 Nov 2022 10:14:33 +0100 Subject: [PATCH 098/132] Prettier --- nextflow_schema.json | 55 +++++++------------------------------------- 1 file changed, 8 insertions(+), 47 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index fd0a031..ea47d39 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,11 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "databases", - "outdir" - ], + "required": ["input", "databases", "outdir"], "properties": { "input": { "type": "string", @@ -63,10 +59,7 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": [ - "fastqc", - "falco" - ], + "enum": ["fastqc", "falco"], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "Specify the tool used for quality control of raw sequencing reads", "fa_icon": "fas fa-tools" @@ -95,10 +88,7 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], + "enum": ["fastp", "adapterremoval"], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -157,11 +147,7 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], + "enum": ["bbduk", "prinseqplusplus", "fastp"], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -195,10 +181,7 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": [ - "entropy", - "dust" - ], + "enum": ["entropy", "dust"], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -372,15 +355,7 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -399,14 +374,7 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], + "enum": ["phylum", "class", "order", "family", "genus", "species"], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -612,14 +580,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { From af8ce2cbfd98ec04e7fd9e74ea0bb2fe58fcede3 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Tue, 29 Nov 2022 10:27:35 +0100 Subject: [PATCH 099/132] chore: update krakenuniq --- modules.json | 2 +- .../krakenuniq/preloadedkrakenuniq/main.nf | 248 +++++++++++++----- .../krakenuniq/preloadedkrakenuniq/meta.yml | 7 +- 3 files changed, 182 insertions(+), 75 deletions(-) diff --git a/modules.json b/modules.json index 8956e42..404ae6d 100644 --- a/modules.json +++ b/modules.json @@ -99,7 +99,7 @@ }, "krakenuniq/preloadedkrakenuniq": { "branch": "master", - "git_sha": "13b9d4854593c03e5e25e8a8f47462542c2c0dd4" + "git_sha": "05649975c6611c6e007537a7984e186e12ae03af" }, "krona/ktimporttaxonomy": { "branch": "master", diff --git a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf index dc9b5a4..0ecacee 100644 --- a/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf +++ b/modules/nf-core/krakenuniq/preloadedkrakenuniq/main.nf @@ -1,4 +1,5 @@ process KRAKENUNIQ_PRELOADEDKRAKENUNIQ { + tag "$meta.id" label 'process_high' conda (params.enable_conda ? "bioconda::krakenuniq=1.0.0" : null) @@ -29,90 +30,195 @@ process KRAKENUNIQ_PRELOADEDKRAKENUNIQ { def args = task.ext.args ?: '' def args2 = task.ext.args ?: '' - def paired = meta.single_end ? "" : "--paired" - def classified = meta.single_end ? '"\$PREFIX".classified.fastq' : '"\$PREFIX".classified#.fastq' - def unclassified = meta.single_end ? '"\$PREFIX".unclassified.fastq' : '"\$PREFIX".unclassified#.fastq' - def classified_option = save_output_fastqs ? "--classified-out ${classified}" : "" - def unclassified_option = save_output_fastqs ? "--unclassified-out ${unclassified}" : "" - def output_option = save_output ? '--output "\$PREFIX".krakenuniq.classified.txt' : "" - def report = report_file ? '--report-file "\$PREFIX".krakenuniq.report.txt' : "" - def compress_reads_command = save_output_fastqs ? "gzip --no-name *.fastq" : "" - - """ - krakenuniq \\ - $args \\ - --db $db \\ - --preload $ram_chunk_size \\ - --threads $task.cpus - -# for fastq in "${fastqs.join('\" \"')}"; do \\ - for fastq in ${fastqs}; do \\ - PREFIX=\$(echo \$fastq) + def classified = meta.single_end ? '"\${PREFIX}.classified.fastq"' : '"\${PREFIX}.classified#.fastq"' + def unclassified = meta.single_end ? '"\${PREFIX}.unclassified.fastq"' : '"\${PREFIX}.unclassified#.fastq"' + def classified_option = save_output_fastqs ? "--classified-out ${classified}" : '' + def unclassified_option = save_output_fastqs ? "--unclassified-out ${unclassified}" : '' + def output_option = save_output ? '--output "\${PREFIX}.krakenuniq.classified.txt"' : '' + def report = report_file ? '--report-file "\${PREFIX}.krakenuniq.report.txt"' : '' + def compress_reads_command = save_output_fastqs ? 'gzip --no-name *.fastq' : '' + if (meta.single_end) { + """ krakenuniq \\ --db $db \\ + --preload \\ + --preload-size $ram_chunk_size \\ --threads $task.cpus \\ - $report \\ - $output_option \\ - $unclassified_option \\ - $classified_option \\ - $output_option \\ - $paired \\ - $args2 \\ - \$fastq - done + $args - $compress_reads_command + strip_suffix() { + local result=\$1 + # Strip any file extensions. + echo "\${result%%.*}" + } - cat <<-END_VERSIONS > versions.yml - "${task.process}": - krakenuniq: \$(echo \$(krakenuniq --version 2>&1) | sed 's/^.*KrakenUniq version //; s/ .*\$//') - END_VERSIONS - """ + printf "%s\\n" ${fastqs} | while read FASTQ; do \\ + PREFIX="\$(strip_suffix "\${FASTQ}")" + + krakenuniq \\ + --db $db \\ + --threads $task.cpus \\ + $report \\ + $output_option \\ + $unclassified_option \\ + $classified_option \\ + $output_option \\ + $args2 \\ + "\${FASTQ}" + done + + $compress_reads_command + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + krakenuniq: \$(echo \$(krakenuniq --version 2>&1) | sed 's/^.*KrakenUniq version //; s/ .*\$//') + END_VERSIONS + """ + } else { + """ + krakenuniq \\ + --db $db \\ + --preload \\ + --preload-size $ram_chunk_size \\ + --threads $task.cpus \\ + $args + + strip_suffix() { + local result + read result + # Strip any trailing dot or underscore. + result="\${result%_}" + echo "\${result%.}" + } + + printf "%s %s\\n" ${fastqs} | while read FASTQ; do \\ + read -r -a FASTQ <<< "\${FASTQ}" + PREFIX="\$(printf "%s\\n" "\${FASTQ[@]}" | sed -e 'N;s/^\\(.*\\).*\\n\\1.*\$/\\1\\n\\1/;D' | strip_suffix)" + + krakenuniq \\ + --db $db \\ + --threads $task.cpus \\ + $report \\ + $output_option \\ + $unclassified_option \\ + $classified_option \\ + $output_option \\ + --paired \\ + $args2 \\ + "\${FASTQ[@]}" + done + + $compress_reads_command + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + krakenuniq: \$(echo \$(krakenuniq --version 2>&1) | sed 's/^.*KrakenUniq version //; s/ .*\$//') + END_VERSIONS + """ + } stub: def args = task.ext.args ?: '' def args2 = task.ext.args ?: '' - def paired = meta.single_end ? "" : "--paired" - def classified = meta.single_end ? '"\$PREFIX".classified.fastq' : '"\$PREFIX".classified#.fastq' - def unclassified = meta.single_end ? '"\$PREFIX".unclassified.fastq' : '"\$PREFIX".unclassified#.fastq' - def classified_option = save_output_fastqs ? "--classified-out ${classified}" : "" - def unclassified_option = save_output_fastqs ? "--unclassified-out ${unclassified}" : "" - def output_option = save_output ? '--output "\$PREFIX".krakenuniq.classified.txt' : "" - def report = report_file ? '--report-file "\$PREFIX".krakenuniq.report.txt' : "" - def compress_reads_command = save_output_fastqs ? "echo 'gzip --no-name *.fastq'" : "" - """ - echo "krakenuniq \\ - $args \\ - --db $db \\ - --preload $ram_chunk_size \\ - --threads $task.cpus" - - for fastq in "${fastqs.join('\" \"')}"; do \\ - PREFIX=\$(echo \$fastq) - echo "krakenuniq \\ + def classified = meta.single_end ? '"\${PREFIX}.classified.fastq"' : '"\${PREFIX}.classified#.fastq"' + def unclassified = meta.single_end ? '"\${PREFIX}.unclassified.fastq"' : '"\${PREFIX}.unclassified#.fastq"' + def classified_option = save_output_fastqs ? "--classified-out ${classified}" : '' + def unclassified_option = save_output_fastqs ? "--unclassified-out ${unclassified}" : '' + def output_option = save_output ? '--output "\${PREFIX}.krakenuniq.classified.txt"' : '' + def report = report_file ? '--report-file "\${PREFIX}.krakenuniq.report.txt"' : '' + def compress_reads_command = save_output_fastqs ? 'gzip --no-name *.fastq' : '' + if (meta.single_end) { + """ + echo krakenuniq \\ --db $db \\ + --preload \\ + --preload-size $ram_chunk_size \\ --threads $task.cpus \\ - $report \\ - $output_option \\ - $unclassified_option \\ - $classified_option \\ - $output_option \\ - $paired \\ - $args2 \\ - \$fastq" + $args - touch "\$PREFIX".classified.fastq.gz - touch "\$PREFIX".krakenuniq.classified.txt - touch "\$PREFIX".krakenuniq.report.txt - touch "\$PREFIX".unclassified.fastq.gz - done + strip_suffix() { + local result=\$1 + # Strip any file extensions. + echo "\${result%%.*}" + } - $compress_reads_command + printf "%s\\n" ${fastqs} | while read FASTQ; do \\ + echo "\${FASTQ}" + PREFIX="\$(strip_suffix "\${FASTQ}")" + echo "\${PREFIX}" - cat <<-END_VERSIONS > versions.yml - "${task.process}": - krakenuniq: \$(echo \$(krakenuniq --version 2>&1) | sed 's/^.*KrakenUniq version //; s/ .*\$//') - END_VERSIONS - """ + echo krakenuniq \\ + --db $db \\ + --threads $task.cpus \\ + $report \\ + $output_option \\ + $unclassified_option \\ + $classified_option \\ + $output_option \\ + $args2 \\ + "\${FASTQ}" + + touch "\${PREFIX}.classified.fastq.gz" + touch "\${PREFIX}.krakenuniq.classified.txt" + touch "\${PREFIX}.krakenuniq.report.txt" + touch "\${PREFIX}.unclassified.fastq.gz" + done + + echo $compress_reads_command + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + krakenuniq: \$(echo \$(krakenuniq --version 2>&1) | sed 's/^.*KrakenUniq version //; s/ .*\$//') + END_VERSIONS + """ + } else { + """ + echo krakenuniq \\ + --db $db \\ + --preload \\ + --preload-size $ram_chunk_size \\ + --threads $task.cpus \\ + $args + + strip_suffix() { + local result + read result + # Strip any trailing dot or underscore. + result="\${result%_}" + echo "\${result%.}" + } + + printf "%s %s\\n" ${fastqs} | while read FASTQ; do \\ + read -r -a FASTQ <<< "\${FASTQ}" + echo "\${FASTQ[@]}" + PREFIX="\$(printf "%s\\n" "\${FASTQ[@]}" | sed -e 'N;s/^\\(.*\\).*\\n\\1.*\$/\\1\\n\\1/;D' | strip_suffix)" + echo "\${PREFIX}" + + echo krakenuniq \\ + --db $db \\ + --threads $task.cpus \\ + $report \\ + $output_option \\ + $unclassified_option \\ + $classified_option \\ + $output_option \\ + --paired \\ + $args2 \\ + "\${FASTQ[@]}" + + touch "\${PREFIX}.classified_1.fastq.gz" "\${PREFIX}.classified_2.fastq.gz" + touch "\${PREFIX}.krakenuniq.classified.txt" + touch "\${PREFIX}.krakenuniq.report.txt" + touch "\${PREFIX}.unclassified_1.fastq.gz" "\${PREFIX}.unclassified_2.fastq.gz" + done + + echo $compress_reads_command + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + krakenuniq: \$(echo \$(krakenuniq --version 2>&1) | sed 's/^.*KrakenUniq version //; s/ .*\$//') + END_VERSIONS + """ + } } diff --git a/modules/nf-core/krakenuniq/preloadedkrakenuniq/meta.yml b/modules/nf-core/krakenuniq/preloadedkrakenuniq/meta.yml index de788af..4ac645c 100644 --- a/modules/nf-core/krakenuniq/preloadedkrakenuniq/meta.yml +++ b/modules/nf-core/krakenuniq/preloadedkrakenuniq/meta.yml @@ -51,13 +51,13 @@ output: description: | Reads classified as belonging to any of the taxa on the KrakenUniq database. - pattern: "*{fastq.gz}" + pattern: "*.fastq.gz" - unclassified_reads_fastq: type: file description: | Reads not classified to any of the taxa on the KrakenUniq database. - pattern: "*{fastq.gz}" + pattern: "*.fastq.gz" - classified_assignment: type: file description: | @@ -68,10 +68,11 @@ output: description: | KrakenUniq report containing stats about classified and not classifed reads. - pattern: "*.{report.txt}" + pattern: "*.report.txt" - versions: type: file description: File containing software versions pattern: "versions.yml" authors: - "@mjamy" + - "@Midnighter" From 49af33f68ea7ab216db898adc24fe2489a8c2f03 Mon Sep 17 00:00:00 2001 From: sofstam Date: Tue, 29 Nov 2022 11:07:29 +0100 Subject: [PATCH 100/132] Include NCBI_id, read counts and mgc arguments in motus --- conf/modules.config | 1 + conf/test_motus.config | 3 +++ nextflow.config | 3 +++ 3 files changed, 7 insertions(+) diff --git a/conf/modules.config b/conf/modules.config index f9d51d8..5c35525 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -446,6 +446,7 @@ process { } withName: MOTUS_PROFILE { + ext.args = { [ params.save_ncbi_id ? "-p" : "", params.save_read_counts ? "-c" : "", params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" ].join(',').replaceAll(','," ") } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/motus/${meta.db_name}/" }, diff --git a/conf/test_motus.config b/conf/test_motus.config index d167b94..70681dd 100644 --- a/conf/test_motus.config +++ b/conf/test_motus.config @@ -39,5 +39,8 @@ params { run_centrifuge = false run_diamond = false run_motus = true + save_mgc_counts = true + save_ncbi_id = true + save_read_counts = true run_profile_standardisation = true } diff --git a/nextflow.config b/nextflow.config index c59d433..91875b2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -140,6 +140,9 @@ params { // mOTUs run_motus = false + save_mgc_counts = false + save_ncbi_id = false + save_read_counts = false // krona run_krona = false From 0235ef42ca0bf062648bd1b1f7bc18827c545a65 Mon Sep 17 00:00:00 2001 From: sofstam Date: Tue, 29 Nov 2022 11:15:40 +0100 Subject: [PATCH 101/132] Update nextflow_schema.json --- nextflow_schema.json | 69 ++++++++++++++++++++++++++++++++++++++------ 1 file changed, 60 insertions(+), 9 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index a66b0f1..5df56d8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,11 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "databases", "outdir"], + "required": [ + "input", + "databases", + "outdir" + ], "properties": { "input": { "type": "string", @@ -80,7 +84,10 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": ["fastp", "adapterremoval"], + "enum": [ + "fastp", + "adapterremoval" + ], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -139,7 +146,11 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], + "enum": [ + "bbduk", + "prinseqplusplus", + "fastp" + ], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -173,7 +184,10 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": ["entropy", "dust"], + "enum": [ + "entropy", + "dust" + ], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -347,7 +361,15 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "enum": [ + "blast", + "xml", + "txt", + "daa", + "sam", + "tsv", + "paf" + ], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -366,7 +388,14 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], + "enum": [ + "phylum", + "class", + "order", + "family", + "genus", + "species" + ], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -572,7 +601,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { @@ -729,9 +765,24 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": ["fastqc", "falco"], + "enum": [ + "fastqc", + "falco" + ], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "Specify the tool used for quality control of raw sequencing reads" + }, + "save_mgc_counts": { + "type": "string", + "description": "Save the mgc reads count in mOTUs." + }, + "save_ncbi_id": { + "type": "string", + "description": "Print NCBI id in mOTUs." + }, + "save_read_counts": { + "type": "string", + "description": "Print result as counts instead of relative abundances in mOTUs." } } -} +} \ No newline at end of file From 5254b8d679b64b903984c2d6e21fea9580efacca Mon Sep 17 00:00:00 2001 From: sofstam Date: Tue, 29 Nov 2022 11:25:52 +0100 Subject: [PATCH 102/132] Prettier --- nextflow_schema.json | 1518 +++++++++++++++++++++--------------------- 1 file changed, 743 insertions(+), 775 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 5df56d8..58cab0c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,788 +1,756 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/taxprofiler/master/nextflow_schema.json", - "title": "nf-core/taxprofiler pipeline parameters", - "description": "Taxonomic profiling of shotgun metagenomic data", - "type": "object", - "definitions": { - "input_output_options": { - "title": "Input/output options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "databases", - "outdir" - ], - "properties": { - "input": { - "type": "string", - "format": "file-path", - "mimetype": "text/csv", - "pattern": "^\\S+\\.(csv|yaml|yml)$", - "schema": "assets/schema_input.json", - "description": "Path to comma-separated file containing information about the samples and libraries/runs.", - "help_text": "You will need to create a design file with information about the samples and libraries/runs you want to running in your pipeline run. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" - }, - "databases": { - "type": "string", - "mimetype": "text/csv", - "format": "file-path", - "fa_icon": "fas fa-database", - "description": "Path to comma-separated file containing information about databases and profiling parameters for each taxonomic profiler", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/dev/usage#full-database-sheet).\n\nProfilers will only be executed if a corresponding database are supplied. \n\nWe recommend storing this database sheet somewhere centrally and accessible by others members of your lab/institutions, as this file will likely be regularly reused." - }, - "outdir": { - "type": "string", - "format": "directory-path", - "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" - }, - "email": { - "type": "string", - "description": "Email address for completion summary.", - "fa_icon": "fas fa-envelope", - "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" - }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" - } - } + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/taxprofiler/master/nextflow_schema.json", + "title": "nf-core/taxprofiler pipeline parameters", + "description": "Taxonomic profiling of shotgun metagenomic data", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["input", "databases", "outdir"], + "properties": { + "input": { + "type": "string", + "format": "file-path", + "mimetype": "text/csv", + "pattern": "^\\S+\\.(csv|yaml|yml)$", + "schema": "assets/schema_input.json", + "description": "Path to comma-separated file containing information about the samples and libraries/runs.", + "help_text": "You will need to create a design file with information about the samples and libraries/runs you want to running in your pipeline run. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" }, - "preprocessing_general_qc_options": { - "title": "Preprocessing general QC options", - "type": "object", - "description": "Common options across both long and short read preprocessing QC steps", - "default": "", - "properties": { - "save_preprocessed_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save reads from adapter clipping/pair-merging, length filtering for both short and long reads", - "help_text": "This saves the FASTQ output from the following tools:\n\n- fastp\n- AdapterRemoval\n- Porechop\n- Filtlong\n\nThese reads will be a mixture of: adapter clipped, quality trimmed, pair-merged, and length filtered, depending on the parameters you set." - } - }, - "fa_icon": "fas fa-users-cog" + "databases": { + "type": "string", + "mimetype": "text/csv", + "format": "file-path", + "fa_icon": "fas fa-database", + "description": "Path to comma-separated file containing information about databases and profiling parameters for each taxonomic profiler", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/dev/usage#full-database-sheet).\n\nProfilers will only be executed if a corresponding database are supplied. \n\nWe recommend storing this database sheet somewhere centrally and accessible by others members of your lab/institutions, as this file will likely be regularly reused." }, - "preprocessing_short_read_qc_options": { - "title": "Preprocessing short-read QC options", - "type": "object", - "description": "Options for adapter clipping, quality trimming, pair-merging, and complexity filtering", - "default": "", - "properties": { - "perform_shortread_qc": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)", - "help_text": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)\n\nThis subworkflow can perform:\n\n- Adapter removal\n- Read quality trimming\n- Read pair merging\n- Length filtering\n- Complexity filtering\n\nEither with fastp or AdapterRemoval.\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences. Note that some, but not all, tools support paired-end alignment (utilising information about the insert covered by the pairs). However read pair merging in some cases can be recommend to increase read length (such as in aDNA). Length filtering, and/or complexity can speed up alignment by reducing the number of short unspecific reads that need to be aligned." - }, - "shortread_qc_tool": { - "type": "string", - "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], - "fa_icon": "fas fa-tools", - "description": "Specify which tool to use for short-read QC" - }, - "shortread_qc_skipadaptertrim": { - "type": "boolean", - "fa_icon": "fas fa-forward", - "description": "Skip adapter trimming", - "help_text": "Skip the removal of sequencing adapters. \n\nThis often can be useful to speed up run-time of the pipeline when analysing data downloaded from public databases such as the ENA or SRA, as adapters should already be removed (however we recommend to check FastQC results to ensure this is the case)." - }, - "shortread_qc_adapter1": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-grip-lines", - "description": "Specify adapter 1 nucleotide sequence", - "help_text": "Specify a custom forward or R1 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG`" - }, - "shortread_qc_adapter2": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-grip-lines", - "description": "Specify adapter 2 nucleotide sequence", - "help_text": "Specify a custom reverse or R2 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT`" - }, - "shortread_qc_adapterlist": { - "type": "string", - "default": "None", - "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", - "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possible combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" - }, - "shortread_qc_mergepairs": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on merging of read pairs for paired-end data", - "default": true, - "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" - }, - "shortread_qc_excludeunmerged": { - "type": "boolean", - "fa_icon": "far fa-times-circle", - "description": "Discard unmerged reads from paired-end merging", - "help_text": "Turns off the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nThis can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - removed from reads `--include_unmerged`\n" - }, - "shortread_qc_minlength": { - "type": "integer", - "default": 15, - "fa_icon": "fas fa-ruler-horizontal", - "description": "Specify the minimum length of reads to be retained", - "help_text": "Specifying a mimum read length filtering can speed up profiling by reducing the number of short unspecific reads that need to be match/aligned to the database.\n\n> Modifies tool parameter(s):\n> - removed from reads `--length_required`\n> - AdapterRemoval: `--minlength`" - }, - "perform_shortread_complexityfilter": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on nucleotide sequence complexity filtering", - "help_text": "Turns on sequencing complexity filtering. Complexity filtering can be useful to increase run-time by removing unspecific read sequences that do not provide any informative taxon ID." - }, - "shortread_complexityfilter_tool": { - "type": "string", - "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], - "fa_icon": "fas fa-hammer", - "description": "Specify which tool to use for complexity filtering" - }, - "shortread_complexityfilter_entropy": { - "type": "number", - "default": 0.3, - "fa_icon": "fas fa-random", - "description": "Specify the minimum sequence entropy level for complexity filtering", - "help_text": "Specify the minimum 'entropy' value for complexity filtering for BBDuk or PRINSEQ++.\n\nNote that this value will only be used for PRINSEQ++ if `--shortread_complexityfilter_prinseqplusplus_mode` is set to `entropy`.\n\nEntropy here corresponds to the amount of sequence variation exists within the read. Higher values correspond to more variety, and thus will likely reslut in more specific matching to a taxon's reference genome. The trade off here is fewer reads (or abundance information) available for having a confident identification.\n\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropy=`\n> - PRINSEQ++: `-lc_entropy`\n\n" - }, - "shortread_complexityfilter_bbduk_windowsize": { - "type": "integer", - "default": 50, - "fa_icon": "far fa-window-maximize", - "description": "Specify the window size for BBDuk complexity filtering", - "help_text": "Specify the window size to calculate the level entropy within for BBDuk.\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropywindow=`" - }, - "shortread_complexityfilter_bbduk_mask": { - "type": "boolean", - "fa_icon": "fas fa-mask", - "description": "Turn on masking rather than discarding of low complexity reads for BBduk", - "help_text": "Turn on masking of low-complexity reads (i.e., replacement with `N`) rather than removal.\n\n> Modifies tool parameter(s)\n> - BBDuk: `entropymask=`" - }, - "shortread_complexityfilter_fastp_threshold": { - "type": "integer", - "default": 30, - "fa_icon": "fas fa-sort-numeric-down", - "description": "Specify the minimum complexity filter threshold of fastp", - "help_text": "Specify the minimum sequence complexity value for fastp. This value corresponds to the percentage of bases that is different from it's adjacent bases.\n\n> Modifies tool parameter(s):\n> - removed from reads `--complexity_threshold`" - }, - "shortread_complexityfilter_prinseqplusplus_mode": { - "type": "string", - "default": "entropy", - "enum": [ - "entropy", - "dust" - ], - "fa_icon": "fas fa-check-square", - "description": "Specify the complexity filter mode for PRINSEQ++" - }, - "shortread_complexityfilter_prinseqplusplus_dustscore": { - "type": "number", - "default": 0.5, - "fa_icon": "fas fa-head-side-mask", - "description": "Specify the minimum dust score for PRINTSEQ++ complexity filtering", - "help_text": "Specify the minimum dust score below which low-complexity reads will be removed. A DUST score is based on how often different tri-nucleotides occur along a read.\n\n> Modifies tool parameter(s):\n> - PRINSEQ++: `--lc_dust`" - }, - "save_complexityfiltered_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save complexity filtered short-reads", - "help_text": "Specify whether to save the final complexity filtered reads in your results directory (`--outdir`)." - } - }, - "fa_icon": "fas fa-compress-alt" + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" }, - "preprocessing_long_read_qc_options": { - "title": "Preprocessing long-read QC options", - "type": "object", - "description": "Options for adapter clipping, quality trimming, and length filtering", - "default": "", - "properties": { - "perform_longread_qc": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on long read quality control steps (adapter clipping, length filtering etc.)", - "help_text": "Turns on long read quality control steps (adapter clipping, length and/or quality filtering.)\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences.\n\nLength filtering, and quality filtering can speed up alignment by reducing the number of unspecific reads that need to be aligned." - }, - "longread_qc_skipadaptertrim": { - "type": "boolean", - "description": "Skip long-read trimming", - "fa_icon": "fas fa-forward", - "help_text": "Skip removal of adapters by Porechop. This can be useful in some cases to speed up run time - particularly when you are running data downloading from public databases such as the ENA/SRA that should already have adapters removed. We recommend that you check your FastQC results this is indeed the case." - }, - "longread_qc_skipqualityfilter": { - "type": "boolean", - "description": "Skip long-read length and quality filtering", - "fa_icon": "fas fa-forward", - "help_text": "Skip removal of quality filtering with Filtlong. This will skip length, percent reads, and target bases filtering (see other `--longread_qc_qualityfilter_*` parameters)." - }, - "longread_qc_qualityfilter_minlength": { - "type": "integer", - "default": 1000, - "description": "Specify the minimum length of reads to be retained", - "fa_icon": "fas fa-ruler-horizontal", - "help_text": "Specify the minimum of length of reads to be kept for downstream analysis.\n\n> Modifies tool parameter(s):\n> - Filtlong: `--min_length`" - }, - "longread_qc_qualityfilter_keeppercent": { - "type": "integer", - "default": 90, - "description": "Specify the percent of high-quality bases to be retained", - "fa_icon": "fas fa-percentage", - "help_text": "Throw out the remaining percentage of reads outside the value. This is measured by bp, not by read count. So this option throws out the worst e.g. 10% of read bases if the parameter is set to `90`. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" - }, - "longread_qc_qualityfilter_targetbases": { - "type": "integer", - "default": 500000000, - "description": "Specify the number of high-quality bases in the library to be retained", - "fa_icon": "fas fa-bullseye", - "help_text": "Removes the worst reads until only the specified value of bases remain, useful for very large read sets. If the input read set is less than the specified value, this setting will have no effect. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" - } - }, - "fa_icon": "fas fa-expand-alt" + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" }, - "preprocessing_host_removal_options": { - "title": "Preprocessing host removal options", - "type": "object", - "description": "Options for pre-profiling host read removal", - "default": "", - "properties": { - "perform_shortread_hostremoval": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on short-read host removal", - "help_text": "Turns on the ability to remove short-reads from the that derived from a known organism, using Bowtie2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." - }, - "perform_longread_hostremoval": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on long-read host removal", - "help_text": "Turns on the ability to remove long-reads from the that derived from a known organism, using minimap2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." - }, - "hostremoval_reference": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-file-alt", - "description": "Specify path to single reference FASTA of host(s) genome(s)", - "help_text": "Specify a path to the FASTA file (optionally gzipped) of the reference genome of the organism to be removed.\n\nIf you have two or more host organisms or contaminants you wish to remove, you can concatenate the FASTAs of the different taxa into a single one to provide to the pipeline." - }, - "shortread_hostremoval_index": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-address-book", - "description": "Specify path to the directory containing pre-made BowTie2 indexes of the host removal reference", - "help_text": "Specify the path to a _directory_ containing pre-made Bowtie2 reference index files (i.e. the directory containing `.bt1`, `.bt2` files etc.). These should sit in the same directory alongside the the reference file specified in `--hostremoval_reference`.\n\nSpecifying premade indices can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." - }, - "longread_hostremoval_index": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-address-book", - "description": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference", - "help_text": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference file given to `--hostremoval_reference`.\n\nSpecifying a premade index file can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." - }, - "save_hostremoval_index": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save mapping index of input reference when not already supplied by user", - "help_text": "Save the output files of the in-built indexing of the host genome.\n\nThis is recommend to be turned on if you plan to use the same reference genome multiple times, as supplying the directory or file to `--shortread_hostremoval_index` or `--longread_hostremoval_index` respectively can speed up runtime of future runs. Once generated, we recommend you place this file _outside_ of your run results directory in a central 'cache' directory you and others using your machine can access and supply to the pipeline." - }, - "save_hostremoval_mapped": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save mapped reads in BAM format from host removal", - "help_text": "Save the reads mapped to the reference genome in BAM format as output by the respective hostremoval alignment tool.\n\nThis can be useful if you wish to perform other analyses on the host organism (such as host-microbe interaction), however, you should consider whether the default mapping parameters of Bowtie2 (short-read) or minimap2 (long-read) are optimised to your context. " - }, - "save_hostremoval_unmapped": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save unmapped reads in FASTQ format from host removal", - "help_text": "Save the unreads mapped to the reference genome in FASTQ format (as exported from `samtools view`).\n\nThis can be useful if you wish to perform other analyses on the off-target reads from the host mapping, such as manual profiling or _de novo_ assembly." - } - }, - "fa_icon": "fas fa-user-times" - }, - "preprocessing_run_merging_options": { - "title": "Preprocessing run merging options", - "type": "object", - "description": "Options for per-sample run-merging", - "default": "", - "properties": { - "perform_runmerging": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on run merging", - "help_text": "Turns on the concatenation of sequencing runs or libraries with the same sample name.\n\nThis can be useful to ensure you get a single profile per sample, rather than one profile per run or library. Note that in some cases comparing profiles of independent _libraries_ may be useful, so this parameter may not always be suitable. " - }, - "save_runmerged_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save run-concatenated input FASTQ files for each sample", - "help_text": "Save the run- and library-concatenated reads of a given sample in FASTQ format." - } - }, - "fa_icon": "fas fa-clipboard-check" - }, - "profiling_options": { - "title": "Profiling options", - "type": "object", - "description": "", - "default": "", - "properties": { - "run_centrifuge": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Centrifuge. Requires database to be present CSV file passed to --databases" - }, - "centrifuge_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Centrifuge-aligned reads", - "help_text": "Save mapped (SAM, FASTQ) and unmapped (FASTQ) reads from alignment step of centrifuge in your output results directory.\n\n> Modifies tool parameter(s):\n> - centrifuge: `--un-gz`, `--al-gz`, `--un-conc-gz`, `--al-conc-gz`, `--out-fmt`" - }, - "run_diamond": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with DIAMOND. Requires database to be present CSV file passed to --databases" - }, - "diamond_output_format": { - "type": "string", - "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], - "fa_icon": "fas fa-file", - "description": "Specify output format from DIAMOND profiling.", - "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" - }, - "diamond_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of DIAMOND-aligned reads. Will override --diamond_output_format and no taxon tables will be generated", - "help_text": "Save aligned reads in SAM format from alignment step of DIAMOND in your output results directory.\n\nNote this explicitly overrides `--diamond_output_format` to produce the SAM file, and no taxon table will be generated.\n\n> Modifies tool parameter(s):\n> - DIAMOND: `--outfmt`" - }, - "run_kaiju": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Kaiju. Requires database to be present CSV file passed to --databases" - }, - "kaiju_taxon_rank": { - "type": "string", - "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], - "fa_icon": "fas fa-tag", - "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", - "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" - }, - "run_kraken2": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Kraken2. Requires database to be present CSV file passed to --databases" - }, - "kraken2_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Kraken2-aligned reads", - "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - kraken2: `--classified-out` and `--unclassified-out`" - }, - "kraken2_save_readclassification": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Kraken2 per-read taxonomic assignment file", - "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - kraken2: `--output`" - }, - "kraken2_save_minimizers": { - "type": "boolean", - "description": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.", - "fa_icon": "fas fa-save", - "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." - }, - "run_bracken": { - "type": "boolean", - "description": "Post-process kraken2 reports with Bracken.", - "fa_icon": "fas fa-toggle-on" - }, - "run_malt": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with MALT. Requires database to be present CSV file passed to --databases" - }, - "malt_mode": { - "type": "string", - "default": "BlastN", - "fa_icon": "fas fa-check-square", - "description": "Specify which MALT alignment mode to use", - "help_text": "Specify which version of MALT alignment to use.\n\nBlastN is generally recommended (nucleotide-nucleotide alignment), but particularly for very short reads (such as aDNA), whereas BlastX mode is similar to DIAMOND and will translate the nucleotide to amino acid sequences. Note each type of alignment mode requires different parameters during database construction. Refer to the MALT manual for more information.\n\n> Modifies tool parameter(s):\n> - malt-run: `-mode` " - }, - "malt_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of MALT-aligned reads", - "help_text": "Turns on saving of MALT aligned reads in SAM format.\n\nNote that the SAM format produce by MALT is not completely valid, and may not work with downstream tools.\n\n> Modifies tool parameter(s):\n> - malt-run: `--alignments`, `-za`" - }, - "malt_generate_megansummary": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on generation of MEGAN summary file from MALT results", - "help_text": "Turns on saving of MALT output in an additional MEGAN summary file (`.megan`) that can be loaded into the MEGAN metagenomic exploration tool.\n\nNote: this file is generated not directly from MALT but rather then MEGAN utility script `rma2info`.\n\n> Modifies tool parameter(s):\n> - rma2info: `-es`" - }, - "run_metaphlan3": { - "type": "boolean", - "description": "Turn on profiling with MetaPhlAn3. Requires database to be present CSV file passed to --databases", - "fa_icon": "fas fa-toggle-on" - }, - "run_motus": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" - } - }, - "fa_icon": "fas fa-align-center" - }, - "postprocessing_and_visualisation_options": { - "title": "Postprocessing and visualisation options", - "type": "object", - "description": "", - "default": "", - "properties": { - "run_profile_standardisation": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on standardisation of taxon tables across profilers", - "help_text": "Turns on standardisation of output OTU tables across all tools; each into a TSV format following the following scheme:\n\n|TAXON | SAMPLE_A | SAMPLE_B |\n|-------------|----------------|-----------------|\n| taxon_a | 32 | 123 |\n| taxon_b | 1 | 5 |\n\nThis currently only is generated for mOTUs." - }, - "generate_biom_output": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on generation of BIOM output (currently only applies to mOTUs)", - "help_text": "Turn on the saving of the taxonomic output in BIOM format (`.biom`) in the results directory of your pipeline run, instead of the default TSV format.\n\nNote this file is from the output of the `motus merge` command.\n\n> Modifies tool parameter(s):\n> - `-B -o`" - }, - "run_krona": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on generation of Krona plots for supported profilers", - "help_text": "Turn on the generation of Krona interactive pie-chart HTMLs for a selection of profilers.\n\nThe tools currently supported are:\n\n- centrifuge\n- kraken2\n- kaiju\n- MALT" - }, - "krona_taxonomy_directory": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-folder-open", - "description": "Specify path to krona taxonomy directories (required for MALT krona plots)", - "help_text": "Specify a path to a Krona taxonomy database directory (i.e. a directory containing a krona generated `.tab` file).\n\nThis is only required for generating Krona plots of MALT output.\n\nNote this taxonomy database must be downloaded and generated with the `updateTaxonomy.sh` script from the krona-tools package." - } - }, - "fa_icon": "fas fa-chart-line" - }, - "institutional_config_options": { - "title": "Institutional config options", - "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", - "properties": { - "custom_config_version": { - "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "custom_config_base": { - "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", - "fa_icon": "fas fa-users-cog" - }, - "config_profile_name": { - "type": "string", - "description": "Institutional config name.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_description": { - "type": "string", - "description": "Institutional config description.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - } - } - }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } - }, - "generic_options": { - "title": "Generic options", - "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", - "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, - "publish_dir_mode": { - "type": "string", - "default": "copy", - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], - "hidden": true - }, - "email_on_fail": { - "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", - "hidden": true - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", - "hidden": true - }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true - }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", - "hidden": true - }, - "hook_url": { - "type": "string", - "description": "Incoming hook URL for messaging service", - "fa_icon": "fas fa-people-group", - "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", - "hidden": true - }, - "multiqc_config": { - "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "multiqc_logo": { - "type": "string", - "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", - "fa_icon": "fas fa-image", - "hidden": true - }, - "multiqc_methods_description": { - "type": "string", - "description": "Custom MultiQC yaml file containing HTML including a methods description.", - "fa_icon": "fas fa-cog" - }, - "tracedir": { - "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "show_hidden_params": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "enable_conda": { - "type": "boolean", - "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", - "hidden": true, - "fa_icon": "fas fa-bacon" - } - } - }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", - "hidden": true - }, - "igenomes_base": { - "type": "string", - "format": "directory-path", - "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes", - "fa_icon": "fas fa-cloud-download-alt", - "hidden": true - }, - "igenomes_ignore": { - "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." - } - } + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" } + } }, - "allOf": [ - { - "$ref": "#/definitions/input_output_options" - }, - { - "$ref": "#/definitions/preprocessing_general_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_short_read_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_long_read_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_host_removal_options" - }, - { - "$ref": "#/definitions/preprocessing_run_merging_options" - }, - { - "$ref": "#/definitions/profiling_options" - }, - { - "$ref": "#/definitions/postprocessing_and_visualisation_options" - }, - { - "$ref": "#/definitions/institutional_config_options" - }, - { - "$ref": "#/definitions/max_job_request_options" - }, - { - "$ref": "#/definitions/generic_options" - }, - { - "$ref": "#/definitions/reference_genome_options" + "preprocessing_general_qc_options": { + "title": "Preprocessing general QC options", + "type": "object", + "description": "Common options across both long and short read preprocessing QC steps", + "default": "", + "properties": { + "save_preprocessed_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save reads from adapter clipping/pair-merging, length filtering for both short and long reads", + "help_text": "This saves the FASTQ output from the following tools:\n\n- fastp\n- AdapterRemoval\n- Porechop\n- Filtlong\n\nThese reads will be a mixture of: adapter clipped, quality trimmed, pair-merged, and length filtered, depending on the parameters you set." } - ], - "properties": { - "preprocessing_qc_tool": { - "type": "string", - "default": "fastqc", - "enum": [ - "fastqc", - "falco" - ], - "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", - "description": "Specify the tool used for quality control of raw sequencing reads" + }, + "fa_icon": "fas fa-users-cog" + }, + "preprocessing_short_read_qc_options": { + "title": "Preprocessing short-read QC options", + "type": "object", + "description": "Options for adapter clipping, quality trimming, pair-merging, and complexity filtering", + "default": "", + "properties": { + "perform_shortread_qc": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)", + "help_text": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)\n\nThis subworkflow can perform:\n\n- Adapter removal\n- Read quality trimming\n- Read pair merging\n- Length filtering\n- Complexity filtering\n\nEither with fastp or AdapterRemoval.\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences. Note that some, but not all, tools support paired-end alignment (utilising information about the insert covered by the pairs). However read pair merging in some cases can be recommend to increase read length (such as in aDNA). Length filtering, and/or complexity can speed up alignment by reducing the number of short unspecific reads that need to be aligned." }, - "save_mgc_counts": { - "type": "string", - "description": "Save the mgc reads count in mOTUs." + "shortread_qc_tool": { + "type": "string", + "default": "fastp", + "enum": ["fastp", "adapterremoval"], + "fa_icon": "fas fa-tools", + "description": "Specify which tool to use for short-read QC" }, - "save_ncbi_id": { - "type": "string", - "description": "Print NCBI id in mOTUs." + "shortread_qc_skipadaptertrim": { + "type": "boolean", + "fa_icon": "fas fa-forward", + "description": "Skip adapter trimming", + "help_text": "Skip the removal of sequencing adapters. \n\nThis often can be useful to speed up run-time of the pipeline when analysing data downloaded from public databases such as the ENA or SRA, as adapters should already be removed (however we recommend to check FastQC results to ensure this is the case)." }, - "save_read_counts": { - "type": "string", - "description": "Print result as counts instead of relative abundances in mOTUs." + "shortread_qc_adapter1": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-grip-lines", + "description": "Specify adapter 1 nucleotide sequence", + "help_text": "Specify a custom forward or R1 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG`" + }, + "shortread_qc_adapter2": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-grip-lines", + "description": "Specify adapter 2 nucleotide sequence", + "help_text": "Specify a custom reverse or R2 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT`" + }, + "shortread_qc_adapterlist": { + "type": "string", + "default": "None", + "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", + "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possible combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" + }, + "shortread_qc_mergepairs": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on merging of read pairs for paired-end data", + "default": true, + "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" + }, + "shortread_qc_excludeunmerged": { + "type": "boolean", + "fa_icon": "far fa-times-circle", + "description": "Discard unmerged reads from paired-end merging", + "help_text": "Turns off the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nThis can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - removed from reads `--include_unmerged`\n" + }, + "shortread_qc_minlength": { + "type": "integer", + "default": 15, + "fa_icon": "fas fa-ruler-horizontal", + "description": "Specify the minimum length of reads to be retained", + "help_text": "Specifying a mimum read length filtering can speed up profiling by reducing the number of short unspecific reads that need to be match/aligned to the database.\n\n> Modifies tool parameter(s):\n> - removed from reads `--length_required`\n> - AdapterRemoval: `--minlength`" + }, + "perform_shortread_complexityfilter": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on nucleotide sequence complexity filtering", + "help_text": "Turns on sequencing complexity filtering. Complexity filtering can be useful to increase run-time by removing unspecific read sequences that do not provide any informative taxon ID." + }, + "shortread_complexityfilter_tool": { + "type": "string", + "default": "bbduk", + "enum": ["bbduk", "prinseqplusplus", "fastp"], + "fa_icon": "fas fa-hammer", + "description": "Specify which tool to use for complexity filtering" + }, + "shortread_complexityfilter_entropy": { + "type": "number", + "default": 0.3, + "fa_icon": "fas fa-random", + "description": "Specify the minimum sequence entropy level for complexity filtering", + "help_text": "Specify the minimum 'entropy' value for complexity filtering for BBDuk or PRINSEQ++.\n\nNote that this value will only be used for PRINSEQ++ if `--shortread_complexityfilter_prinseqplusplus_mode` is set to `entropy`.\n\nEntropy here corresponds to the amount of sequence variation exists within the read. Higher values correspond to more variety, and thus will likely reslut in more specific matching to a taxon's reference genome. The trade off here is fewer reads (or abundance information) available for having a confident identification.\n\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropy=`\n> - PRINSEQ++: `-lc_entropy`\n\n" + }, + "shortread_complexityfilter_bbduk_windowsize": { + "type": "integer", + "default": 50, + "fa_icon": "far fa-window-maximize", + "description": "Specify the window size for BBDuk complexity filtering", + "help_text": "Specify the window size to calculate the level entropy within for BBDuk.\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropywindow=`" + }, + "shortread_complexityfilter_bbduk_mask": { + "type": "boolean", + "fa_icon": "fas fa-mask", + "description": "Turn on masking rather than discarding of low complexity reads for BBduk", + "help_text": "Turn on masking of low-complexity reads (i.e., replacement with `N`) rather than removal.\n\n> Modifies tool parameter(s)\n> - BBDuk: `entropymask=`" + }, + "shortread_complexityfilter_fastp_threshold": { + "type": "integer", + "default": 30, + "fa_icon": "fas fa-sort-numeric-down", + "description": "Specify the minimum complexity filter threshold of fastp", + "help_text": "Specify the minimum sequence complexity value for fastp. This value corresponds to the percentage of bases that is different from it's adjacent bases.\n\n> Modifies tool parameter(s):\n> - removed from reads `--complexity_threshold`" + }, + "shortread_complexityfilter_prinseqplusplus_mode": { + "type": "string", + "default": "entropy", + "enum": ["entropy", "dust"], + "fa_icon": "fas fa-check-square", + "description": "Specify the complexity filter mode for PRINSEQ++" + }, + "shortread_complexityfilter_prinseqplusplus_dustscore": { + "type": "number", + "default": 0.5, + "fa_icon": "fas fa-head-side-mask", + "description": "Specify the minimum dust score for PRINTSEQ++ complexity filtering", + "help_text": "Specify the minimum dust score below which low-complexity reads will be removed. A DUST score is based on how often different tri-nucleotides occur along a read.\n\n> Modifies tool parameter(s):\n> - PRINSEQ++: `--lc_dust`" + }, + "save_complexityfiltered_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save complexity filtered short-reads", + "help_text": "Specify whether to save the final complexity filtered reads in your results directory (`--outdir`)." } + }, + "fa_icon": "fas fa-compress-alt" + }, + "preprocessing_long_read_qc_options": { + "title": "Preprocessing long-read QC options", + "type": "object", + "description": "Options for adapter clipping, quality trimming, and length filtering", + "default": "", + "properties": { + "perform_longread_qc": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on long read quality control steps (adapter clipping, length filtering etc.)", + "help_text": "Turns on long read quality control steps (adapter clipping, length and/or quality filtering.)\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences.\n\nLength filtering, and quality filtering can speed up alignment by reducing the number of unspecific reads that need to be aligned." + }, + "longread_qc_skipadaptertrim": { + "type": "boolean", + "description": "Skip long-read trimming", + "fa_icon": "fas fa-forward", + "help_text": "Skip removal of adapters by Porechop. This can be useful in some cases to speed up run time - particularly when you are running data downloading from public databases such as the ENA/SRA that should already have adapters removed. We recommend that you check your FastQC results this is indeed the case." + }, + "longread_qc_skipqualityfilter": { + "type": "boolean", + "description": "Skip long-read length and quality filtering", + "fa_icon": "fas fa-forward", + "help_text": "Skip removal of quality filtering with Filtlong. This will skip length, percent reads, and target bases filtering (see other `--longread_qc_qualityfilter_*` parameters)." + }, + "longread_qc_qualityfilter_minlength": { + "type": "integer", + "default": 1000, + "description": "Specify the minimum length of reads to be retained", + "fa_icon": "fas fa-ruler-horizontal", + "help_text": "Specify the minimum of length of reads to be kept for downstream analysis.\n\n> Modifies tool parameter(s):\n> - Filtlong: `--min_length`" + }, + "longread_qc_qualityfilter_keeppercent": { + "type": "integer", + "default": 90, + "description": "Specify the percent of high-quality bases to be retained", + "fa_icon": "fas fa-percentage", + "help_text": "Throw out the remaining percentage of reads outside the value. This is measured by bp, not by read count. So this option throws out the worst e.g. 10% of read bases if the parameter is set to `90`. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" + }, + "longread_qc_qualityfilter_targetbases": { + "type": "integer", + "default": 500000000, + "description": "Specify the number of high-quality bases in the library to be retained", + "fa_icon": "fas fa-bullseye", + "help_text": "Removes the worst reads until only the specified value of bases remain, useful for very large read sets. If the input read set is less than the specified value, this setting will have no effect. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" + } + }, + "fa_icon": "fas fa-expand-alt" + }, + "preprocessing_host_removal_options": { + "title": "Preprocessing host removal options", + "type": "object", + "description": "Options for pre-profiling host read removal", + "default": "", + "properties": { + "perform_shortread_hostremoval": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on short-read host removal", + "help_text": "Turns on the ability to remove short-reads from the that derived from a known organism, using Bowtie2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." + }, + "perform_longread_hostremoval": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on long-read host removal", + "help_text": "Turns on the ability to remove long-reads from the that derived from a known organism, using minimap2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." + }, + "hostremoval_reference": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-file-alt", + "description": "Specify path to single reference FASTA of host(s) genome(s)", + "help_text": "Specify a path to the FASTA file (optionally gzipped) of the reference genome of the organism to be removed.\n\nIf you have two or more host organisms or contaminants you wish to remove, you can concatenate the FASTAs of the different taxa into a single one to provide to the pipeline." + }, + "shortread_hostremoval_index": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-address-book", + "description": "Specify path to the directory containing pre-made BowTie2 indexes of the host removal reference", + "help_text": "Specify the path to a _directory_ containing pre-made Bowtie2 reference index files (i.e. the directory containing `.bt1`, `.bt2` files etc.). These should sit in the same directory alongside the the reference file specified in `--hostremoval_reference`.\n\nSpecifying premade indices can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." + }, + "longread_hostremoval_index": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-address-book", + "description": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference", + "help_text": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference file given to `--hostremoval_reference`.\n\nSpecifying a premade index file can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." + }, + "save_hostremoval_index": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save mapping index of input reference when not already supplied by user", + "help_text": "Save the output files of the in-built indexing of the host genome.\n\nThis is recommend to be turned on if you plan to use the same reference genome multiple times, as supplying the directory or file to `--shortread_hostremoval_index` or `--longread_hostremoval_index` respectively can speed up runtime of future runs. Once generated, we recommend you place this file _outside_ of your run results directory in a central 'cache' directory you and others using your machine can access and supply to the pipeline." + }, + "save_hostremoval_mapped": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save mapped reads in BAM format from host removal", + "help_text": "Save the reads mapped to the reference genome in BAM format as output by the respective hostremoval alignment tool.\n\nThis can be useful if you wish to perform other analyses on the host organism (such as host-microbe interaction), however, you should consider whether the default mapping parameters of Bowtie2 (short-read) or minimap2 (long-read) are optimised to your context. " + }, + "save_hostremoval_unmapped": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save unmapped reads in FASTQ format from host removal", + "help_text": "Save the unreads mapped to the reference genome in FASTQ format (as exported from `samtools view`).\n\nThis can be useful if you wish to perform other analyses on the off-target reads from the host mapping, such as manual profiling or _de novo_ assembly." + } + }, + "fa_icon": "fas fa-user-times" + }, + "preprocessing_run_merging_options": { + "title": "Preprocessing run merging options", + "type": "object", + "description": "Options for per-sample run-merging", + "default": "", + "properties": { + "perform_runmerging": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on run merging", + "help_text": "Turns on the concatenation of sequencing runs or libraries with the same sample name.\n\nThis can be useful to ensure you get a single profile per sample, rather than one profile per run or library. Note that in some cases comparing profiles of independent _libraries_ may be useful, so this parameter may not always be suitable. " + }, + "save_runmerged_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save run-concatenated input FASTQ files for each sample", + "help_text": "Save the run- and library-concatenated reads of a given sample in FASTQ format." + } + }, + "fa_icon": "fas fa-clipboard-check" + }, + "profiling_options": { + "title": "Profiling options", + "type": "object", + "description": "", + "default": "", + "properties": { + "run_centrifuge": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Centrifuge. Requires database to be present CSV file passed to --databases" + }, + "centrifuge_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Centrifuge-aligned reads", + "help_text": "Save mapped (SAM, FASTQ) and unmapped (FASTQ) reads from alignment step of centrifuge in your output results directory.\n\n> Modifies tool parameter(s):\n> - centrifuge: `--un-gz`, `--al-gz`, `--un-conc-gz`, `--al-conc-gz`, `--out-fmt`" + }, + "run_diamond": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with DIAMOND. Requires database to be present CSV file passed to --databases" + }, + "diamond_output_format": { + "type": "string", + "default": "tsv", + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "fa_icon": "fas fa-file", + "description": "Specify output format from DIAMOND profiling.", + "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" + }, + "diamond_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of DIAMOND-aligned reads. Will override --diamond_output_format and no taxon tables will be generated", + "help_text": "Save aligned reads in SAM format from alignment step of DIAMOND in your output results directory.\n\nNote this explicitly overrides `--diamond_output_format` to produce the SAM file, and no taxon table will be generated.\n\n> Modifies tool parameter(s):\n> - DIAMOND: `--outfmt`" + }, + "run_kaiju": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Kaiju. Requires database to be present CSV file passed to --databases" + }, + "kaiju_taxon_rank": { + "type": "string", + "default": "species", + "enum": ["phylum", "class", "order", "family", "genus", "species"], + "fa_icon": "fas fa-tag", + "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", + "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" + }, + "run_kraken2": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Kraken2. Requires database to be present CSV file passed to --databases" + }, + "kraken2_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Kraken2-aligned reads", + "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - kraken2: `--classified-out` and `--unclassified-out`" + }, + "kraken2_save_readclassification": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Kraken2 per-read taxonomic assignment file", + "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - kraken2: `--output`" + }, + "kraken2_save_minimizers": { + "type": "boolean", + "description": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.", + "fa_icon": "fas fa-save", + "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." + }, + "run_bracken": { + "type": "boolean", + "description": "Post-process kraken2 reports with Bracken.", + "fa_icon": "fas fa-toggle-on" + }, + "run_malt": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with MALT. Requires database to be present CSV file passed to --databases" + }, + "malt_mode": { + "type": "string", + "default": "BlastN", + "fa_icon": "fas fa-check-square", + "description": "Specify which MALT alignment mode to use", + "help_text": "Specify which version of MALT alignment to use.\n\nBlastN is generally recommended (nucleotide-nucleotide alignment), but particularly for very short reads (such as aDNA), whereas BlastX mode is similar to DIAMOND and will translate the nucleotide to amino acid sequences. Note each type of alignment mode requires different parameters during database construction. Refer to the MALT manual for more information.\n\n> Modifies tool parameter(s):\n> - malt-run: `-mode` " + }, + "malt_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of MALT-aligned reads", + "help_text": "Turns on saving of MALT aligned reads in SAM format.\n\nNote that the SAM format produce by MALT is not completely valid, and may not work with downstream tools.\n\n> Modifies tool parameter(s):\n> - malt-run: `--alignments`, `-za`" + }, + "malt_generate_megansummary": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on generation of MEGAN summary file from MALT results", + "help_text": "Turns on saving of MALT output in an additional MEGAN summary file (`.megan`) that can be loaded into the MEGAN metagenomic exploration tool.\n\nNote: this file is generated not directly from MALT but rather then MEGAN utility script `rma2info`.\n\n> Modifies tool parameter(s):\n> - rma2info: `-es`" + }, + "run_metaphlan3": { + "type": "boolean", + "description": "Turn on profiling with MetaPhlAn3. Requires database to be present CSV file passed to --databases", + "fa_icon": "fas fa-toggle-on" + }, + "run_motus": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" + } + }, + "fa_icon": "fas fa-align-center" + }, + "postprocessing_and_visualisation_options": { + "title": "Postprocessing and visualisation options", + "type": "object", + "description": "", + "default": "", + "properties": { + "run_profile_standardisation": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on standardisation of taxon tables across profilers", + "help_text": "Turns on standardisation of output OTU tables across all tools; each into a TSV format following the following scheme:\n\n|TAXON | SAMPLE_A | SAMPLE_B |\n|-------------|----------------|-----------------|\n| taxon_a | 32 | 123 |\n| taxon_b | 1 | 5 |\n\nThis currently only is generated for mOTUs." + }, + "generate_biom_output": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on generation of BIOM output (currently only applies to mOTUs)", + "help_text": "Turn on the saving of the taxonomic output in BIOM format (`.biom`) in the results directory of your pipeline run, instead of the default TSV format.\n\nNote this file is from the output of the `motus merge` command.\n\n> Modifies tool parameter(s):\n> - `-B -o`" + }, + "run_krona": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on generation of Krona plots for supported profilers", + "help_text": "Turn on the generation of Krona interactive pie-chart HTMLs for a selection of profilers.\n\nThe tools currently supported are:\n\n- centrifuge\n- kraken2\n- kaiju\n- MALT" + }, + "krona_taxonomy_directory": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-folder-open", + "description": "Specify path to krona taxonomy directories (required for MALT krona plots)", + "help_text": "Specify a path to a Krona taxonomy database directory (i.e. a directory containing a krona generated `.tab` file).\n\nThis is only required for generating Krona plots of MALT output.\n\nNote this taxonomy database must be downloaded and generated with the `updateTaxonomy.sh` script from the krona-tools package." + } + }, + "fa_icon": "fas fa-chart-line" + }, + "institutional_config_options": { + "title": "Institutional config options", + "type": "object", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "properties": { + "custom_config_version": { + "type": "string", + "description": "Git commit id for Institutional configs.", + "default": "master", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "custom_config_base": { + "type": "string", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", + "hidden": true, + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" + }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], + "hidden": true + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "hook_url": { + "type": "string", + "description": "Incoming hook URL for messaging service", + "fa_icon": "fas fa-people-group", + "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", + "hidden": true + }, + "multiqc_config": { + "type": "string", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_logo": { + "type": "string", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", + "hidden": true + }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, + "tracedir": { + "type": "string", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", + "hidden": true, + "fa_icon": "fas fa-bacon" + } + } + }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Reference genome related files and options required for the workflow.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", + "hidden": true + }, + "igenomes_base": { + "type": "string", + "format": "directory-path", + "description": "Directory / URL base for iGenomes references.", + "default": "s3://ngi-igenomes/igenomes", + "fa_icon": "fas fa-cloud-download-alt", + "hidden": true + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } } -} \ No newline at end of file + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/preprocessing_general_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_short_read_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_long_read_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_host_removal_options" + }, + { + "$ref": "#/definitions/preprocessing_run_merging_options" + }, + { + "$ref": "#/definitions/profiling_options" + }, + { + "$ref": "#/definitions/postprocessing_and_visualisation_options" + }, + { + "$ref": "#/definitions/institutional_config_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/generic_options" + }, + { + "$ref": "#/definitions/reference_genome_options" + } + ], + "properties": { + "preprocessing_qc_tool": { + "type": "string", + "default": "fastqc", + "enum": ["fastqc", "falco"], + "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", + "description": "Specify the tool used for quality control of raw sequencing reads" + }, + "save_mgc_counts": { + "type": "boolean", + "description": "Save the mgc reads count in mOTUs." + }, + "save_ncbi_id": { + "type": "boolean", + "description": "Print NCBI id in mOTUs." + }, + "save_read_counts": { + "type": "boolean", + "description": "Print result as counts instead of relative abundances in mOTUs." + } + } +} From 644a29c699fd4c9e9b1b500570342b795cfcbe4a Mon Sep 17 00:00:00 2001 From: sofstam Date: Tue, 29 Nov 2022 11:34:57 +0100 Subject: [PATCH 103/132] Prettier --- nextflow_schema.json | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 58cab0c..cbe70b0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -742,15 +742,15 @@ }, "save_mgc_counts": { "type": "boolean", - "description": "Save the mgc reads count in mOTUs." + "description": "Save the mgc reads count for mOTUs." }, "save_ncbi_id": { "type": "boolean", - "description": "Print NCBI id in mOTUs." + "description": "Print NCBI taxonomic id for mOTUs." }, "save_read_counts": { "type": "boolean", - "description": "Print result as counts instead of relative abundances in mOTUs." + "description": "Print mOTUs result as counts instead of relative abundances.." } } } From fb6be69d7eefd55ec52f53daf6059ee59ffa2c4f Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Tue, 29 Nov 2022 11:37:20 +0100 Subject: [PATCH 104/132] Prettier should work now --- nextflow_schema.json | 1477 +++++++++++++++++++++--------------------- 1 file changed, 735 insertions(+), 742 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index cbe70b0..e0b7fa6 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,756 +1,749 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/taxprofiler/master/nextflow_schema.json", - "title": "nf-core/taxprofiler pipeline parameters", - "description": "Taxonomic profiling of shotgun metagenomic data", - "type": "object", - "definitions": { - "input_output_options": { - "title": "Input/output options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "databases", "outdir"], - "properties": { - "input": { - "type": "string", - "format": "file-path", - "mimetype": "text/csv", - "pattern": "^\\S+\\.(csv|yaml|yml)$", - "schema": "assets/schema_input.json", - "description": "Path to comma-separated file containing information about the samples and libraries/runs.", - "help_text": "You will need to create a design file with information about the samples and libraries/runs you want to running in your pipeline run. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/taxprofiler/master/nextflow_schema.json", + "title": "nf-core/taxprofiler pipeline parameters", + "description": "Taxonomic profiling of shotgun metagenomic data", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["input", "databases", "outdir"], + "properties": { + "input": { + "type": "string", + "format": "file-path", + "mimetype": "text/csv", + "pattern": "^\\S+\\.(csv|yaml|yml)$", + "schema": "assets/schema_input.json", + "description": "Path to comma-separated file containing information about the samples and libraries/runs.", + "help_text": "You will need to create a design file with information about the samples and libraries/runs you want to running in your pipeline run. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" + }, + "databases": { + "type": "string", + "mimetype": "text/csv", + "format": "file-path", + "fa_icon": "fas fa-database", + "description": "Path to comma-separated file containing information about databases and profiling parameters for each taxonomic profiler", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/dev/usage#full-database-sheet).\n\nProfilers will only be executed if a corresponding database are supplied. \n\nWe recommend storing this database sheet somewhere centrally and accessible by others members of your lab/institutions, as this file will likely be regularly reused." + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" + } + } }, - "databases": { - "type": "string", - "mimetype": "text/csv", - "format": "file-path", - "fa_icon": "fas fa-database", - "description": "Path to comma-separated file containing information about databases and profiling parameters for each taxonomic profiler", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/dev/usage#full-database-sheet).\n\nProfilers will only be executed if a corresponding database are supplied. \n\nWe recommend storing this database sheet somewhere centrally and accessible by others members of your lab/institutions, as this file will likely be regularly reused." + "preprocessing_general_qc_options": { + "title": "Preprocessing general QC options", + "type": "object", + "description": "Common options across both long and short read preprocessing QC steps", + "default": "", + "properties": { + "save_preprocessed_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save reads from adapter clipping/pair-merging, length filtering for both short and long reads", + "help_text": "This saves the FASTQ output from the following tools:\n\n- fastp\n- AdapterRemoval\n- Porechop\n- Filtlong\n\nThese reads will be a mixture of: adapter clipped, quality trimmed, pair-merged, and length filtered, depending on the parameters you set." + } + }, + "fa_icon": "fas fa-users-cog" }, - "outdir": { - "type": "string", - "format": "directory-path", - "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" + "preprocessing_short_read_qc_options": { + "title": "Preprocessing short-read QC options", + "type": "object", + "description": "Options for adapter clipping, quality trimming, pair-merging, and complexity filtering", + "default": "", + "properties": { + "perform_shortread_qc": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)", + "help_text": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)\n\nThis subworkflow can perform:\n\n- Adapter removal\n- Read quality trimming\n- Read pair merging\n- Length filtering\n- Complexity filtering\n\nEither with fastp or AdapterRemoval.\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences. Note that some, but not all, tools support paired-end alignment (utilising information about the insert covered by the pairs). However read pair merging in some cases can be recommend to increase read length (such as in aDNA). Length filtering, and/or complexity can speed up alignment by reducing the number of short unspecific reads that need to be aligned." + }, + "shortread_qc_tool": { + "type": "string", + "default": "fastp", + "enum": ["fastp", "adapterremoval"], + "fa_icon": "fas fa-tools", + "description": "Specify which tool to use for short-read QC" + }, + "shortread_qc_skipadaptertrim": { + "type": "boolean", + "fa_icon": "fas fa-forward", + "description": "Skip adapter trimming", + "help_text": "Skip the removal of sequencing adapters. \n\nThis often can be useful to speed up run-time of the pipeline when analysing data downloaded from public databases such as the ENA or SRA, as adapters should already be removed (however we recommend to check FastQC results to ensure this is the case)." + }, + "shortread_qc_adapter1": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-grip-lines", + "description": "Specify adapter 1 nucleotide sequence", + "help_text": "Specify a custom forward or R1 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG`" + }, + "shortread_qc_adapter2": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-grip-lines", + "description": "Specify adapter 2 nucleotide sequence", + "help_text": "Specify a custom reverse or R2 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT`" + }, + "shortread_qc_adapterlist": { + "type": "string", + "default": "None", + "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", + "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possible combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" + }, + "shortread_qc_mergepairs": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on merging of read pairs for paired-end data", + "default": true, + "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" + }, + "shortread_qc_excludeunmerged": { + "type": "boolean", + "fa_icon": "far fa-times-circle", + "description": "Discard unmerged reads from paired-end merging", + "help_text": "Turns off the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nThis can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - removed from reads `--include_unmerged`\n" + }, + "shortread_qc_minlength": { + "type": "integer", + "default": 15, + "fa_icon": "fas fa-ruler-horizontal", + "description": "Specify the minimum length of reads to be retained", + "help_text": "Specifying a mimum read length filtering can speed up profiling by reducing the number of short unspecific reads that need to be match/aligned to the database.\n\n> Modifies tool parameter(s):\n> - removed from reads `--length_required`\n> - AdapterRemoval: `--minlength`" + }, + "perform_shortread_complexityfilter": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on nucleotide sequence complexity filtering", + "help_text": "Turns on sequencing complexity filtering. Complexity filtering can be useful to increase run-time by removing unspecific read sequences that do not provide any informative taxon ID." + }, + "shortread_complexityfilter_tool": { + "type": "string", + "default": "bbduk", + "enum": ["bbduk", "prinseqplusplus", "fastp"], + "fa_icon": "fas fa-hammer", + "description": "Specify which tool to use for complexity filtering" + }, + "shortread_complexityfilter_entropy": { + "type": "number", + "default": 0.3, + "fa_icon": "fas fa-random", + "description": "Specify the minimum sequence entropy level for complexity filtering", + "help_text": "Specify the minimum 'entropy' value for complexity filtering for BBDuk or PRINSEQ++.\n\nNote that this value will only be used for PRINSEQ++ if `--shortread_complexityfilter_prinseqplusplus_mode` is set to `entropy`.\n\nEntropy here corresponds to the amount of sequence variation exists within the read. Higher values correspond to more variety, and thus will likely reslut in more specific matching to a taxon's reference genome. The trade off here is fewer reads (or abundance information) available for having a confident identification.\n\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropy=`\n> - PRINSEQ++: `-lc_entropy`\n\n" + }, + "shortread_complexityfilter_bbduk_windowsize": { + "type": "integer", + "default": 50, + "fa_icon": "far fa-window-maximize", + "description": "Specify the window size for BBDuk complexity filtering", + "help_text": "Specify the window size to calculate the level entropy within for BBDuk.\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropywindow=`" + }, + "shortread_complexityfilter_bbduk_mask": { + "type": "boolean", + "fa_icon": "fas fa-mask", + "description": "Turn on masking rather than discarding of low complexity reads for BBduk", + "help_text": "Turn on masking of low-complexity reads (i.e., replacement with `N`) rather than removal.\n\n> Modifies tool parameter(s)\n> - BBDuk: `entropymask=`" + }, + "shortread_complexityfilter_fastp_threshold": { + "type": "integer", + "default": 30, + "fa_icon": "fas fa-sort-numeric-down", + "description": "Specify the minimum complexity filter threshold of fastp", + "help_text": "Specify the minimum sequence complexity value for fastp. This value corresponds to the percentage of bases that is different from it's adjacent bases.\n\n> Modifies tool parameter(s):\n> - removed from reads `--complexity_threshold`" + }, + "shortread_complexityfilter_prinseqplusplus_mode": { + "type": "string", + "default": "entropy", + "enum": ["entropy", "dust"], + "fa_icon": "fas fa-check-square", + "description": "Specify the complexity filter mode for PRINSEQ++" + }, + "shortread_complexityfilter_prinseqplusplus_dustscore": { + "type": "number", + "default": 0.5, + "fa_icon": "fas fa-head-side-mask", + "description": "Specify the minimum dust score for PRINTSEQ++ complexity filtering", + "help_text": "Specify the minimum dust score below which low-complexity reads will be removed. A DUST score is based on how often different tri-nucleotides occur along a read.\n\n> Modifies tool parameter(s):\n> - PRINSEQ++: `--lc_dust`" + }, + "save_complexityfiltered_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save complexity filtered short-reads", + "help_text": "Specify whether to save the final complexity filtered reads in your results directory (`--outdir`)." + } + }, + "fa_icon": "fas fa-compress-alt" }, - "email": { - "type": "string", - "description": "Email address for completion summary.", - "fa_icon": "fas fa-envelope", - "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + "preprocessing_long_read_qc_options": { + "title": "Preprocessing long-read QC options", + "type": "object", + "description": "Options for adapter clipping, quality trimming, and length filtering", + "default": "", + "properties": { + "perform_longread_qc": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on long read quality control steps (adapter clipping, length filtering etc.)", + "help_text": "Turns on long read quality control steps (adapter clipping, length and/or quality filtering.)\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences.\n\nLength filtering, and quality filtering can speed up alignment by reducing the number of unspecific reads that need to be aligned." + }, + "longread_qc_skipadaptertrim": { + "type": "boolean", + "description": "Skip long-read trimming", + "fa_icon": "fas fa-forward", + "help_text": "Skip removal of adapters by Porechop. This can be useful in some cases to speed up run time - particularly when you are running data downloading from public databases such as the ENA/SRA that should already have adapters removed. We recommend that you check your FastQC results this is indeed the case." + }, + "longread_qc_skipqualityfilter": { + "type": "boolean", + "description": "Skip long-read length and quality filtering", + "fa_icon": "fas fa-forward", + "help_text": "Skip removal of quality filtering with Filtlong. This will skip length, percent reads, and target bases filtering (see other `--longread_qc_qualityfilter_*` parameters)." + }, + "longread_qc_qualityfilter_minlength": { + "type": "integer", + "default": 1000, + "description": "Specify the minimum length of reads to be retained", + "fa_icon": "fas fa-ruler-horizontal", + "help_text": "Specify the minimum of length of reads to be kept for downstream analysis.\n\n> Modifies tool parameter(s):\n> - Filtlong: `--min_length`" + }, + "longread_qc_qualityfilter_keeppercent": { + "type": "integer", + "default": 90, + "description": "Specify the percent of high-quality bases to be retained", + "fa_icon": "fas fa-percentage", + "help_text": "Throw out the remaining percentage of reads outside the value. This is measured by bp, not by read count. So this option throws out the worst e.g. 10% of read bases if the parameter is set to `90`. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" + }, + "longread_qc_qualityfilter_targetbases": { + "type": "integer", + "default": 500000000, + "description": "Specify the number of high-quality bases in the library to be retained", + "fa_icon": "fas fa-bullseye", + "help_text": "Removes the worst reads until only the specified value of bases remain, useful for very large read sets. If the input read set is less than the specified value, this setting will have no effect. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" + } + }, + "fa_icon": "fas fa-expand-alt" }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" + "preprocessing_host_removal_options": { + "title": "Preprocessing host removal options", + "type": "object", + "description": "Options for pre-profiling host read removal", + "default": "", + "properties": { + "perform_shortread_hostremoval": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on short-read host removal", + "help_text": "Turns on the ability to remove short-reads from the that derived from a known organism, using Bowtie2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." + }, + "perform_longread_hostremoval": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on long-read host removal", + "help_text": "Turns on the ability to remove long-reads from the that derived from a known organism, using minimap2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." + }, + "hostremoval_reference": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-file-alt", + "description": "Specify path to single reference FASTA of host(s) genome(s)", + "help_text": "Specify a path to the FASTA file (optionally gzipped) of the reference genome of the organism to be removed.\n\nIf you have two or more host organisms or contaminants you wish to remove, you can concatenate the FASTAs of the different taxa into a single one to provide to the pipeline." + }, + "shortread_hostremoval_index": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-address-book", + "description": "Specify path to the directory containing pre-made BowTie2 indexes of the host removal reference", + "help_text": "Specify the path to a _directory_ containing pre-made Bowtie2 reference index files (i.e. the directory containing `.bt1`, `.bt2` files etc.). These should sit in the same directory alongside the the reference file specified in `--hostremoval_reference`.\n\nSpecifying premade indices can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." + }, + "longread_hostremoval_index": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-address-book", + "description": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference", + "help_text": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference file given to `--hostremoval_reference`.\n\nSpecifying a premade index file can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." + }, + "save_hostremoval_index": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save mapping index of input reference when not already supplied by user", + "help_text": "Save the output files of the in-built indexing of the host genome.\n\nThis is recommend to be turned on if you plan to use the same reference genome multiple times, as supplying the directory or file to `--shortread_hostremoval_index` or `--longread_hostremoval_index` respectively can speed up runtime of future runs. Once generated, we recommend you place this file _outside_ of your run results directory in a central 'cache' directory you and others using your machine can access and supply to the pipeline." + }, + "save_hostremoval_mapped": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save mapped reads in BAM format from host removal", + "help_text": "Save the reads mapped to the reference genome in BAM format as output by the respective hostremoval alignment tool.\n\nThis can be useful if you wish to perform other analyses on the host organism (such as host-microbe interaction), however, you should consider whether the default mapping parameters of Bowtie2 (short-read) or minimap2 (long-read) are optimised to your context. " + }, + "save_hostremoval_unmapped": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save unmapped reads in FASTQ format from host removal", + "help_text": "Save the unreads mapped to the reference genome in FASTQ format (as exported from `samtools view`).\n\nThis can be useful if you wish to perform other analyses on the off-target reads from the host mapping, such as manual profiling or _de novo_ assembly." + } + }, + "fa_icon": "fas fa-user-times" + }, + "preprocessing_run_merging_options": { + "title": "Preprocessing run merging options", + "type": "object", + "description": "Options for per-sample run-merging", + "default": "", + "properties": { + "perform_runmerging": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on run merging", + "help_text": "Turns on the concatenation of sequencing runs or libraries with the same sample name.\n\nThis can be useful to ensure you get a single profile per sample, rather than one profile per run or library. Note that in some cases comparing profiles of independent _libraries_ may be useful, so this parameter may not always be suitable. " + }, + "save_runmerged_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save run-concatenated input FASTQ files for each sample", + "help_text": "Save the run- and library-concatenated reads of a given sample in FASTQ format." + } + }, + "fa_icon": "fas fa-clipboard-check" + }, + "profiling_options": { + "title": "Profiling options", + "type": "object", + "description": "", + "default": "", + "properties": { + "run_centrifuge": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Centrifuge. Requires database to be present CSV file passed to --databases" + }, + "centrifuge_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Centrifuge-aligned reads", + "help_text": "Save mapped (SAM, FASTQ) and unmapped (FASTQ) reads from alignment step of centrifuge in your output results directory.\n\n> Modifies tool parameter(s):\n> - centrifuge: `--un-gz`, `--al-gz`, `--un-conc-gz`, `--al-conc-gz`, `--out-fmt`" + }, + "run_diamond": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with DIAMOND. Requires database to be present CSV file passed to --databases" + }, + "diamond_output_format": { + "type": "string", + "default": "tsv", + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "fa_icon": "fas fa-file", + "description": "Specify output format from DIAMOND profiling.", + "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" + }, + "diamond_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of DIAMOND-aligned reads. Will override --diamond_output_format and no taxon tables will be generated", + "help_text": "Save aligned reads in SAM format from alignment step of DIAMOND in your output results directory.\n\nNote this explicitly overrides `--diamond_output_format` to produce the SAM file, and no taxon table will be generated.\n\n> Modifies tool parameter(s):\n> - DIAMOND: `--outfmt`" + }, + "run_kaiju": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Kaiju. Requires database to be present CSV file passed to --databases" + }, + "kaiju_taxon_rank": { + "type": "string", + "default": "species", + "enum": ["phylum", "class", "order", "family", "genus", "species"], + "fa_icon": "fas fa-tag", + "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", + "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" + }, + "run_kraken2": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Kraken2. Requires database to be present CSV file passed to --databases" + }, + "kraken2_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Kraken2-aligned reads", + "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - kraken2: `--classified-out` and `--unclassified-out`" + }, + "kraken2_save_readclassification": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Kraken2 per-read taxonomic assignment file", + "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - kraken2: `--output`" + }, + "kraken2_save_minimizers": { + "type": "boolean", + "description": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.", + "fa_icon": "fas fa-save", + "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." + }, + "run_bracken": { + "type": "boolean", + "description": "Post-process kraken2 reports with Bracken.", + "fa_icon": "fas fa-toggle-on" + }, + "run_malt": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with MALT. Requires database to be present CSV file passed to --databases" + }, + "malt_mode": { + "type": "string", + "default": "BlastN", + "fa_icon": "fas fa-check-square", + "description": "Specify which MALT alignment mode to use", + "help_text": "Specify which version of MALT alignment to use.\n\nBlastN is generally recommended (nucleotide-nucleotide alignment), but particularly for very short reads (such as aDNA), whereas BlastX mode is similar to DIAMOND and will translate the nucleotide to amino acid sequences. Note each type of alignment mode requires different parameters during database construction. Refer to the MALT manual for more information.\n\n> Modifies tool parameter(s):\n> - malt-run: `-mode` " + }, + "malt_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of MALT-aligned reads", + "help_text": "Turns on saving of MALT aligned reads in SAM format.\n\nNote that the SAM format produce by MALT is not completely valid, and may not work with downstream tools.\n\n> Modifies tool parameter(s):\n> - malt-run: `--alignments`, `-za`" + }, + "malt_generate_megansummary": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on generation of MEGAN summary file from MALT results", + "help_text": "Turns on saving of MALT output in an additional MEGAN summary file (`.megan`) that can be loaded into the MEGAN metagenomic exploration tool.\n\nNote: this file is generated not directly from MALT but rather then MEGAN utility script `rma2info`.\n\n> Modifies tool parameter(s):\n> - rma2info: `-es`" + }, + "run_metaphlan3": { + "type": "boolean", + "description": "Turn on profiling with MetaPhlAn3. Requires database to be present CSV file passed to --databases", + "fa_icon": "fas fa-toggle-on" + }, + "run_motus": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" + } + }, + "fa_icon": "fas fa-align-center" + }, + "postprocessing_and_visualisation_options": { + "title": "Postprocessing and visualisation options", + "type": "object", + "description": "", + "default": "", + "properties": { + "run_profile_standardisation": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on standardisation of taxon tables across profilers", + "help_text": "Turns on standardisation of output OTU tables across all tools; each into a TSV format following the following scheme:\n\n|TAXON | SAMPLE_A | SAMPLE_B |\n|-------------|----------------|-----------------|\n| taxon_a | 32 | 123 |\n| taxon_b | 1 | 5 |\n\nThis currently only is generated for mOTUs." + }, + "generate_biom_output": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on generation of BIOM output (currently only applies to mOTUs)", + "help_text": "Turn on the saving of the taxonomic output in BIOM format (`.biom`) in the results directory of your pipeline run, instead of the default TSV format.\n\nNote this file is from the output of the `motus merge` command.\n\n> Modifies tool parameter(s):\n> - `-B -o`" + }, + "run_krona": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on generation of Krona plots for supported profilers", + "help_text": "Turn on the generation of Krona interactive pie-chart HTMLs for a selection of profilers.\n\nThe tools currently supported are:\n\n- centrifuge\n- kraken2\n- kaiju\n- MALT" + }, + "krona_taxonomy_directory": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-folder-open", + "description": "Specify path to krona taxonomy directories (required for MALT krona plots)", + "help_text": "Specify a path to a Krona taxonomy database directory (i.e. a directory containing a krona generated `.tab` file).\n\nThis is only required for generating Krona plots of MALT output.\n\nNote this taxonomy database must be downloaded and generated with the `updateTaxonomy.sh` script from the krona-tools package." + } + }, + "fa_icon": "fas fa-chart-line" + }, + "institutional_config_options": { + "title": "Institutional config options", + "type": "object", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "properties": { + "custom_config_version": { + "type": "string", + "description": "Git commit id for Institutional configs.", + "default": "master", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "custom_config_base": { + "type": "string", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", + "hidden": true, + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" + }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "hook_url": { + "type": "string", + "description": "Incoming hook URL for messaging service", + "fa_icon": "fas fa-people-group", + "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", + "hidden": true + }, + "multiqc_config": { + "type": "string", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_logo": { + "type": "string", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", + "hidden": true + }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, + "tracedir": { + "type": "string", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", + "hidden": true, + "fa_icon": "fas fa-bacon" + } + } + }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Reference genome related files and options required for the workflow.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", + "hidden": true + }, + "igenomes_base": { + "type": "string", + "format": "directory-path", + "description": "Directory / URL base for iGenomes references.", + "default": "s3://ngi-igenomes/igenomes", + "fa_icon": "fas fa-cloud-download-alt", + "hidden": true + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } } - } }, - "preprocessing_general_qc_options": { - "title": "Preprocessing general QC options", - "type": "object", - "description": "Common options across both long and short read preprocessing QC steps", - "default": "", - "properties": { - "save_preprocessed_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save reads from adapter clipping/pair-merging, length filtering for both short and long reads", - "help_text": "This saves the FASTQ output from the following tools:\n\n- fastp\n- AdapterRemoval\n- Porechop\n- Filtlong\n\nThese reads will be a mixture of: adapter clipped, quality trimmed, pair-merged, and length filtered, depending on the parameters you set." + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/preprocessing_general_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_short_read_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_long_read_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_host_removal_options" + }, + { + "$ref": "#/definitions/preprocessing_run_merging_options" + }, + { + "$ref": "#/definitions/profiling_options" + }, + { + "$ref": "#/definitions/postprocessing_and_visualisation_options" + }, + { + "$ref": "#/definitions/institutional_config_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/generic_options" + }, + { + "$ref": "#/definitions/reference_genome_options" } - }, - "fa_icon": "fas fa-users-cog" - }, - "preprocessing_short_read_qc_options": { - "title": "Preprocessing short-read QC options", - "type": "object", - "description": "Options for adapter clipping, quality trimming, pair-merging, and complexity filtering", - "default": "", - "properties": { - "perform_shortread_qc": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)", - "help_text": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)\n\nThis subworkflow can perform:\n\n- Adapter removal\n- Read quality trimming\n- Read pair merging\n- Length filtering\n- Complexity filtering\n\nEither with fastp or AdapterRemoval.\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences. Note that some, but not all, tools support paired-end alignment (utilising information about the insert covered by the pairs). However read pair merging in some cases can be recommend to increase read length (such as in aDNA). Length filtering, and/or complexity can speed up alignment by reducing the number of short unspecific reads that need to be aligned." + ], + "properties": { + "preprocessing_qc_tool": { + "type": "string", + "default": "fastqc", + "enum": ["fastqc", "falco"], + "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", + "description": "Specify the tool used for quality control of raw sequencing reads" }, - "shortread_qc_tool": { - "type": "string", - "default": "fastp", - "enum": ["fastp", "adapterremoval"], - "fa_icon": "fas fa-tools", - "description": "Specify which tool to use for short-read QC" + "save_mgc_counts": { + "type": "boolean", + "description": "Save the mgc reads count for mOTUs." }, - "shortread_qc_skipadaptertrim": { - "type": "boolean", - "fa_icon": "fas fa-forward", - "description": "Skip adapter trimming", - "help_text": "Skip the removal of sequencing adapters. \n\nThis often can be useful to speed up run-time of the pipeline when analysing data downloaded from public databases such as the ENA or SRA, as adapters should already be removed (however we recommend to check FastQC results to ensure this is the case)." + "save_ncbi_id": { + "type": "boolean", + "description": "Print NCBI taxonomic id for mOTUs." }, - "shortread_qc_adapter1": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-grip-lines", - "description": "Specify adapter 1 nucleotide sequence", - "help_text": "Specify a custom forward or R1 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG`" - }, - "shortread_qc_adapter2": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-grip-lines", - "description": "Specify adapter 2 nucleotide sequence", - "help_text": "Specify a custom reverse or R2 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT`" - }, - "shortread_qc_adapterlist": { - "type": "string", - "default": "None", - "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", - "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possible combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" - }, - "shortread_qc_mergepairs": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on merging of read pairs for paired-end data", - "default": true, - "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" - }, - "shortread_qc_excludeunmerged": { - "type": "boolean", - "fa_icon": "far fa-times-circle", - "description": "Discard unmerged reads from paired-end merging", - "help_text": "Turns off the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nThis can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - removed from reads `--include_unmerged`\n" - }, - "shortread_qc_minlength": { - "type": "integer", - "default": 15, - "fa_icon": "fas fa-ruler-horizontal", - "description": "Specify the minimum length of reads to be retained", - "help_text": "Specifying a mimum read length filtering can speed up profiling by reducing the number of short unspecific reads that need to be match/aligned to the database.\n\n> Modifies tool parameter(s):\n> - removed from reads `--length_required`\n> - AdapterRemoval: `--minlength`" - }, - "perform_shortread_complexityfilter": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on nucleotide sequence complexity filtering", - "help_text": "Turns on sequencing complexity filtering. Complexity filtering can be useful to increase run-time by removing unspecific read sequences that do not provide any informative taxon ID." - }, - "shortread_complexityfilter_tool": { - "type": "string", - "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], - "fa_icon": "fas fa-hammer", - "description": "Specify which tool to use for complexity filtering" - }, - "shortread_complexityfilter_entropy": { - "type": "number", - "default": 0.3, - "fa_icon": "fas fa-random", - "description": "Specify the minimum sequence entropy level for complexity filtering", - "help_text": "Specify the minimum 'entropy' value for complexity filtering for BBDuk or PRINSEQ++.\n\nNote that this value will only be used for PRINSEQ++ if `--shortread_complexityfilter_prinseqplusplus_mode` is set to `entropy`.\n\nEntropy here corresponds to the amount of sequence variation exists within the read. Higher values correspond to more variety, and thus will likely reslut in more specific matching to a taxon's reference genome. The trade off here is fewer reads (or abundance information) available for having a confident identification.\n\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropy=`\n> - PRINSEQ++: `-lc_entropy`\n\n" - }, - "shortread_complexityfilter_bbduk_windowsize": { - "type": "integer", - "default": 50, - "fa_icon": "far fa-window-maximize", - "description": "Specify the window size for BBDuk complexity filtering", - "help_text": "Specify the window size to calculate the level entropy within for BBDuk.\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropywindow=`" - }, - "shortread_complexityfilter_bbduk_mask": { - "type": "boolean", - "fa_icon": "fas fa-mask", - "description": "Turn on masking rather than discarding of low complexity reads for BBduk", - "help_text": "Turn on masking of low-complexity reads (i.e., replacement with `N`) rather than removal.\n\n> Modifies tool parameter(s)\n> - BBDuk: `entropymask=`" - }, - "shortread_complexityfilter_fastp_threshold": { - "type": "integer", - "default": 30, - "fa_icon": "fas fa-sort-numeric-down", - "description": "Specify the minimum complexity filter threshold of fastp", - "help_text": "Specify the minimum sequence complexity value for fastp. This value corresponds to the percentage of bases that is different from it's adjacent bases.\n\n> Modifies tool parameter(s):\n> - removed from reads `--complexity_threshold`" - }, - "shortread_complexityfilter_prinseqplusplus_mode": { - "type": "string", - "default": "entropy", - "enum": ["entropy", "dust"], - "fa_icon": "fas fa-check-square", - "description": "Specify the complexity filter mode for PRINSEQ++" - }, - "shortread_complexityfilter_prinseqplusplus_dustscore": { - "type": "number", - "default": 0.5, - "fa_icon": "fas fa-head-side-mask", - "description": "Specify the minimum dust score for PRINTSEQ++ complexity filtering", - "help_text": "Specify the minimum dust score below which low-complexity reads will be removed. A DUST score is based on how often different tri-nucleotides occur along a read.\n\n> Modifies tool parameter(s):\n> - PRINSEQ++: `--lc_dust`" - }, - "save_complexityfiltered_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save complexity filtered short-reads", - "help_text": "Specify whether to save the final complexity filtered reads in your results directory (`--outdir`)." + "save_read_counts": { + "type": "boolean", + "description": "Print mOTUs result as counts instead of relative abundances.." } - }, - "fa_icon": "fas fa-compress-alt" - }, - "preprocessing_long_read_qc_options": { - "title": "Preprocessing long-read QC options", - "type": "object", - "description": "Options for adapter clipping, quality trimming, and length filtering", - "default": "", - "properties": { - "perform_longread_qc": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on long read quality control steps (adapter clipping, length filtering etc.)", - "help_text": "Turns on long read quality control steps (adapter clipping, length and/or quality filtering.)\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences.\n\nLength filtering, and quality filtering can speed up alignment by reducing the number of unspecific reads that need to be aligned." - }, - "longread_qc_skipadaptertrim": { - "type": "boolean", - "description": "Skip long-read trimming", - "fa_icon": "fas fa-forward", - "help_text": "Skip removal of adapters by Porechop. This can be useful in some cases to speed up run time - particularly when you are running data downloading from public databases such as the ENA/SRA that should already have adapters removed. We recommend that you check your FastQC results this is indeed the case." - }, - "longread_qc_skipqualityfilter": { - "type": "boolean", - "description": "Skip long-read length and quality filtering", - "fa_icon": "fas fa-forward", - "help_text": "Skip removal of quality filtering with Filtlong. This will skip length, percent reads, and target bases filtering (see other `--longread_qc_qualityfilter_*` parameters)." - }, - "longread_qc_qualityfilter_minlength": { - "type": "integer", - "default": 1000, - "description": "Specify the minimum length of reads to be retained", - "fa_icon": "fas fa-ruler-horizontal", - "help_text": "Specify the minimum of length of reads to be kept for downstream analysis.\n\n> Modifies tool parameter(s):\n> - Filtlong: `--min_length`" - }, - "longread_qc_qualityfilter_keeppercent": { - "type": "integer", - "default": 90, - "description": "Specify the percent of high-quality bases to be retained", - "fa_icon": "fas fa-percentage", - "help_text": "Throw out the remaining percentage of reads outside the value. This is measured by bp, not by read count. So this option throws out the worst e.g. 10% of read bases if the parameter is set to `90`. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" - }, - "longread_qc_qualityfilter_targetbases": { - "type": "integer", - "default": 500000000, - "description": "Specify the number of high-quality bases in the library to be retained", - "fa_icon": "fas fa-bullseye", - "help_text": "Removes the worst reads until only the specified value of bases remain, useful for very large read sets. If the input read set is less than the specified value, this setting will have no effect. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" - } - }, - "fa_icon": "fas fa-expand-alt" - }, - "preprocessing_host_removal_options": { - "title": "Preprocessing host removal options", - "type": "object", - "description": "Options for pre-profiling host read removal", - "default": "", - "properties": { - "perform_shortread_hostremoval": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on short-read host removal", - "help_text": "Turns on the ability to remove short-reads from the that derived from a known organism, using Bowtie2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." - }, - "perform_longread_hostremoval": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on long-read host removal", - "help_text": "Turns on the ability to remove long-reads from the that derived from a known organism, using minimap2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." - }, - "hostremoval_reference": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-file-alt", - "description": "Specify path to single reference FASTA of host(s) genome(s)", - "help_text": "Specify a path to the FASTA file (optionally gzipped) of the reference genome of the organism to be removed.\n\nIf you have two or more host organisms or contaminants you wish to remove, you can concatenate the FASTAs of the different taxa into a single one to provide to the pipeline." - }, - "shortread_hostremoval_index": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-address-book", - "description": "Specify path to the directory containing pre-made BowTie2 indexes of the host removal reference", - "help_text": "Specify the path to a _directory_ containing pre-made Bowtie2 reference index files (i.e. the directory containing `.bt1`, `.bt2` files etc.). These should sit in the same directory alongside the the reference file specified in `--hostremoval_reference`.\n\nSpecifying premade indices can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." - }, - "longread_hostremoval_index": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-address-book", - "description": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference", - "help_text": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference file given to `--hostremoval_reference`.\n\nSpecifying a premade index file can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." - }, - "save_hostremoval_index": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save mapping index of input reference when not already supplied by user", - "help_text": "Save the output files of the in-built indexing of the host genome.\n\nThis is recommend to be turned on if you plan to use the same reference genome multiple times, as supplying the directory or file to `--shortread_hostremoval_index` or `--longread_hostremoval_index` respectively can speed up runtime of future runs. Once generated, we recommend you place this file _outside_ of your run results directory in a central 'cache' directory you and others using your machine can access and supply to the pipeline." - }, - "save_hostremoval_mapped": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save mapped reads in BAM format from host removal", - "help_text": "Save the reads mapped to the reference genome in BAM format as output by the respective hostremoval alignment tool.\n\nThis can be useful if you wish to perform other analyses on the host organism (such as host-microbe interaction), however, you should consider whether the default mapping parameters of Bowtie2 (short-read) or minimap2 (long-read) are optimised to your context. " - }, - "save_hostremoval_unmapped": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save unmapped reads in FASTQ format from host removal", - "help_text": "Save the unreads mapped to the reference genome in FASTQ format (as exported from `samtools view`).\n\nThis can be useful if you wish to perform other analyses on the off-target reads from the host mapping, such as manual profiling or _de novo_ assembly." - } - }, - "fa_icon": "fas fa-user-times" - }, - "preprocessing_run_merging_options": { - "title": "Preprocessing run merging options", - "type": "object", - "description": "Options for per-sample run-merging", - "default": "", - "properties": { - "perform_runmerging": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on run merging", - "help_text": "Turns on the concatenation of sequencing runs or libraries with the same sample name.\n\nThis can be useful to ensure you get a single profile per sample, rather than one profile per run or library. Note that in some cases comparing profiles of independent _libraries_ may be useful, so this parameter may not always be suitable. " - }, - "save_runmerged_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save run-concatenated input FASTQ files for each sample", - "help_text": "Save the run- and library-concatenated reads of a given sample in FASTQ format." - } - }, - "fa_icon": "fas fa-clipboard-check" - }, - "profiling_options": { - "title": "Profiling options", - "type": "object", - "description": "", - "default": "", - "properties": { - "run_centrifuge": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Centrifuge. Requires database to be present CSV file passed to --databases" - }, - "centrifuge_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Centrifuge-aligned reads", - "help_text": "Save mapped (SAM, FASTQ) and unmapped (FASTQ) reads from alignment step of centrifuge in your output results directory.\n\n> Modifies tool parameter(s):\n> - centrifuge: `--un-gz`, `--al-gz`, `--un-conc-gz`, `--al-conc-gz`, `--out-fmt`" - }, - "run_diamond": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with DIAMOND. Requires database to be present CSV file passed to --databases" - }, - "diamond_output_format": { - "type": "string", - "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], - "fa_icon": "fas fa-file", - "description": "Specify output format from DIAMOND profiling.", - "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" - }, - "diamond_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of DIAMOND-aligned reads. Will override --diamond_output_format and no taxon tables will be generated", - "help_text": "Save aligned reads in SAM format from alignment step of DIAMOND in your output results directory.\n\nNote this explicitly overrides `--diamond_output_format` to produce the SAM file, and no taxon table will be generated.\n\n> Modifies tool parameter(s):\n> - DIAMOND: `--outfmt`" - }, - "run_kaiju": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Kaiju. Requires database to be present CSV file passed to --databases" - }, - "kaiju_taxon_rank": { - "type": "string", - "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], - "fa_icon": "fas fa-tag", - "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", - "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" - }, - "run_kraken2": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Kraken2. Requires database to be present CSV file passed to --databases" - }, - "kraken2_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Kraken2-aligned reads", - "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - kraken2: `--classified-out` and `--unclassified-out`" - }, - "kraken2_save_readclassification": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Kraken2 per-read taxonomic assignment file", - "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - kraken2: `--output`" - }, - "kraken2_save_minimizers": { - "type": "boolean", - "description": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.", - "fa_icon": "fas fa-save", - "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." - }, - "run_bracken": { - "type": "boolean", - "description": "Post-process kraken2 reports with Bracken.", - "fa_icon": "fas fa-toggle-on" - }, - "run_malt": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with MALT. Requires database to be present CSV file passed to --databases" - }, - "malt_mode": { - "type": "string", - "default": "BlastN", - "fa_icon": "fas fa-check-square", - "description": "Specify which MALT alignment mode to use", - "help_text": "Specify which version of MALT alignment to use.\n\nBlastN is generally recommended (nucleotide-nucleotide alignment), but particularly for very short reads (such as aDNA), whereas BlastX mode is similar to DIAMOND and will translate the nucleotide to amino acid sequences. Note each type of alignment mode requires different parameters during database construction. Refer to the MALT manual for more information.\n\n> Modifies tool parameter(s):\n> - malt-run: `-mode` " - }, - "malt_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of MALT-aligned reads", - "help_text": "Turns on saving of MALT aligned reads in SAM format.\n\nNote that the SAM format produce by MALT is not completely valid, and may not work with downstream tools.\n\n> Modifies tool parameter(s):\n> - malt-run: `--alignments`, `-za`" - }, - "malt_generate_megansummary": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on generation of MEGAN summary file from MALT results", - "help_text": "Turns on saving of MALT output in an additional MEGAN summary file (`.megan`) that can be loaded into the MEGAN metagenomic exploration tool.\n\nNote: this file is generated not directly from MALT but rather then MEGAN utility script `rma2info`.\n\n> Modifies tool parameter(s):\n> - rma2info: `-es`" - }, - "run_metaphlan3": { - "type": "boolean", - "description": "Turn on profiling with MetaPhlAn3. Requires database to be present CSV file passed to --databases", - "fa_icon": "fas fa-toggle-on" - }, - "run_motus": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" - } - }, - "fa_icon": "fas fa-align-center" - }, - "postprocessing_and_visualisation_options": { - "title": "Postprocessing and visualisation options", - "type": "object", - "description": "", - "default": "", - "properties": { - "run_profile_standardisation": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on standardisation of taxon tables across profilers", - "help_text": "Turns on standardisation of output OTU tables across all tools; each into a TSV format following the following scheme:\n\n|TAXON | SAMPLE_A | SAMPLE_B |\n|-------------|----------------|-----------------|\n| taxon_a | 32 | 123 |\n| taxon_b | 1 | 5 |\n\nThis currently only is generated for mOTUs." - }, - "generate_biom_output": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on generation of BIOM output (currently only applies to mOTUs)", - "help_text": "Turn on the saving of the taxonomic output in BIOM format (`.biom`) in the results directory of your pipeline run, instead of the default TSV format.\n\nNote this file is from the output of the `motus merge` command.\n\n> Modifies tool parameter(s):\n> - `-B -o`" - }, - "run_krona": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on generation of Krona plots for supported profilers", - "help_text": "Turn on the generation of Krona interactive pie-chart HTMLs for a selection of profilers.\n\nThe tools currently supported are:\n\n- centrifuge\n- kraken2\n- kaiju\n- MALT" - }, - "krona_taxonomy_directory": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-folder-open", - "description": "Specify path to krona taxonomy directories (required for MALT krona plots)", - "help_text": "Specify a path to a Krona taxonomy database directory (i.e. a directory containing a krona generated `.tab` file).\n\nThis is only required for generating Krona plots of MALT output.\n\nNote this taxonomy database must be downloaded and generated with the `updateTaxonomy.sh` script from the krona-tools package." - } - }, - "fa_icon": "fas fa-chart-line" - }, - "institutional_config_options": { - "title": "Institutional config options", - "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", - "properties": { - "custom_config_version": { - "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "custom_config_base": { - "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", - "fa_icon": "fas fa-users-cog" - }, - "config_profile_name": { - "type": "string", - "description": "Institutional config name.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_description": { - "type": "string", - "description": "Institutional config description.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - } - } - }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } - }, - "generic_options": { - "title": "Generic options", - "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", - "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, - "publish_dir_mode": { - "type": "string", - "default": "copy", - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], - "hidden": true - }, - "email_on_fail": { - "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", - "hidden": true - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", - "hidden": true - }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true - }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", - "hidden": true - }, - "hook_url": { - "type": "string", - "description": "Incoming hook URL for messaging service", - "fa_icon": "fas fa-people-group", - "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", - "hidden": true - }, - "multiqc_config": { - "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "multiqc_logo": { - "type": "string", - "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", - "fa_icon": "fas fa-image", - "hidden": true - }, - "multiqc_methods_description": { - "type": "string", - "description": "Custom MultiQC yaml file containing HTML including a methods description.", - "fa_icon": "fas fa-cog" - }, - "tracedir": { - "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "show_hidden_params": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "enable_conda": { - "type": "boolean", - "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", - "hidden": true, - "fa_icon": "fas fa-bacon" - } - } - }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", - "hidden": true - }, - "igenomes_base": { - "type": "string", - "format": "directory-path", - "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes", - "fa_icon": "fas fa-cloud-download-alt", - "hidden": true - }, - "igenomes_ignore": { - "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." - } - } } - }, - "allOf": [ - { - "$ref": "#/definitions/input_output_options" - }, - { - "$ref": "#/definitions/preprocessing_general_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_short_read_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_long_read_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_host_removal_options" - }, - { - "$ref": "#/definitions/preprocessing_run_merging_options" - }, - { - "$ref": "#/definitions/profiling_options" - }, - { - "$ref": "#/definitions/postprocessing_and_visualisation_options" - }, - { - "$ref": "#/definitions/institutional_config_options" - }, - { - "$ref": "#/definitions/max_job_request_options" - }, - { - "$ref": "#/definitions/generic_options" - }, - { - "$ref": "#/definitions/reference_genome_options" - } - ], - "properties": { - "preprocessing_qc_tool": { - "type": "string", - "default": "fastqc", - "enum": ["fastqc", "falco"], - "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", - "description": "Specify the tool used for quality control of raw sequencing reads" - }, - "save_mgc_counts": { - "type": "boolean", - "description": "Save the mgc reads count for mOTUs." - }, - "save_ncbi_id": { - "type": "boolean", - "description": "Print NCBI taxonomic id for mOTUs." - }, - "save_read_counts": { - "type": "boolean", - "description": "Print mOTUs result as counts instead of relative abundances.." - } - } } From 71b52b9c94a0e009a8ded36dd7ebf2351145adcc Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Tue, 29 Nov 2022 11:58:23 +0100 Subject: [PATCH 105/132] Apply review suggestions --- conf/modules.config | 6 +++++- nextflow_schema.json | 2 +- 2 files changed, 6 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 5c35525..8502ebe 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -446,7 +446,11 @@ process { } withName: MOTUS_PROFILE { - ext.args = { [ params.save_ncbi_id ? "-p" : "", params.save_read_counts ? "-c" : "", params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" ].join(',').replaceAll(','," ") } + ext.args = { [ + params.save_ncbi_id ? "-p" : "", + params.save_read_counts ? "-c" : "", + params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" + ].join(',').replaceAll(','," ") } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/motus/${meta.db_name}/" }, diff --git a/nextflow_schema.json b/nextflow_schema.json index e0b7fa6..8e2250b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -743,7 +743,7 @@ }, "save_read_counts": { "type": "boolean", - "description": "Print mOTUs result as counts instead of relative abundances.." + "description": "Print mOTUs result as counts instead of relative abundances." } } } From ba727a81aef811faa91aa1f85a5e2a40b7101b3e Mon Sep 17 00:00:00 2001 From: Sofia Stamouli Date: Tue, 29 Nov 2022 12:45:14 +0100 Subject: [PATCH 106/132] Fix left-padding spaces --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 8502ebe..7327a2b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -450,7 +450,7 @@ process { params.save_ncbi_id ? "-p" : "", params.save_read_counts ? "-c" : "", params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" - ].join(',').replaceAll(','," ") } + ].join(',').replaceAll(','," ") } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/motus/${meta.db_name}/" }, From 770a35d7aca245ef12f9d820b90d639c398bdfb0 Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Tue, 29 Nov 2022 12:49:20 +0100 Subject: [PATCH 107/132] Update conf/modules.config --- conf/modules.config | 12 +++++++----- 1 file changed, 7 insertions(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 7327a2b..9494e02 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -446,11 +446,13 @@ process { } withName: MOTUS_PROFILE { - ext.args = { [ - params.save_ncbi_id ? "-p" : "", - params.save_read_counts ? "-c" : "", - params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" - ].join(',').replaceAll(','," ") } + ext.args = { + [ + params.save_ncbi_id ? "-p" : "", + params.save_read_counts ? "-c" : "", + params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" + ].join(',').replaceAll(','," ") + } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/motus/${meta.db_name}/" }, From 0088b07220c502da5e568916e1d0e366f9992a26 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Tue, 29 Nov 2022 13:28:04 +0100 Subject: [PATCH 108/132] Fix trailing whitespace --- conf/modules.config | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 9494e02..a2852d5 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -446,12 +446,12 @@ process { } withName: MOTUS_PROFILE { - ext.args = { + ext.args = { [ - params.save_ncbi_id ? "-p" : "", - params.save_read_counts ? "-c" : "", - params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" - ].join(',').replaceAll(','," ") + params.save_ncbi_id ? "-p" : "", + params.save_read_counts ? "-c" : "", + params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" + ].join(',').replaceAll(','," ") } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ From 7e3ece53bd9fd76660b8d1dcc499ea010a382ec1 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Tue, 29 Nov 2022 13:31:00 +0100 Subject: [PATCH 109/132] Update modules.config --- conf/modules.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index a2852d5..0f54301 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -447,12 +447,12 @@ process { withName: MOTUS_PROFILE { ext.args = { - [ + [ params.save_ncbi_id ? "-p" : "", params.save_read_counts ? "-c" : "", params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" ].join(',').replaceAll(','," ") - } + } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/motus/${meta.db_name}/" }, From d7fbe55849f79e4029c746b5457c0ab2d4ba1a98 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 29 Nov 2022 14:19:01 +0100 Subject: [PATCH 110/132] Add KU to the test profiles and add id to KU processes --- conf/test.config | 1 + conf/test_motus.config | 1 + conf/test_nopreprocessing.config | 1 + conf/test_noprofiling.config | 1 + conf/test_nothing.config | 1 + subworkflows/local/profiling.nf | 2 +- 6 files changed, 6 insertions(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index 777d9bf..016eb2e 100644 --- a/conf/test.config +++ b/conf/test.config @@ -39,6 +39,7 @@ params { run_metaphlan3 = true run_centrifuge = true run_diamond = true + run_krakenuniq = true run_motus = false run_krona = true krona_taxonomy_directory = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/sarscov2/metagenome/krona_taxonomy.tab' diff --git a/conf/test_motus.config b/conf/test_motus.config index d167b94..2428a4c 100644 --- a/conf/test_motus.config +++ b/conf/test_motus.config @@ -38,6 +38,7 @@ params { run_metaphlan3 = false run_centrifuge = false run_diamond = false + run_krakenuniq = false run_motus = true run_profile_standardisation = true } diff --git a/conf/test_nopreprocessing.config b/conf/test_nopreprocessing.config index 357f76f..9a51320 100644 --- a/conf/test_nopreprocessing.config +++ b/conf/test_nopreprocessing.config @@ -38,6 +38,7 @@ params { run_metaphlan3 = true run_centrifuge = true run_diamond = true + run_krakenuniq = true run_motus = false run_krona = true } diff --git a/conf/test_noprofiling.config b/conf/test_noprofiling.config index 59ed0da..3ca715b 100644 --- a/conf/test_noprofiling.config +++ b/conf/test_noprofiling.config @@ -39,6 +39,7 @@ params { run_metaphlan3 = false run_centrifuge = false run_diamond = false + run_krakenuniq = false run_motus = false } diff --git a/conf/test_nothing.config b/conf/test_nothing.config index df09613..47976df 100644 --- a/conf/test_nothing.config +++ b/conf/test_nothing.config @@ -38,6 +38,7 @@ params { run_metaphlan3 = false run_centrifuge = false run_diamond = false + run_krakenuniq = false run_motus = false } diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 4ca2c4c..aeee2a4 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -271,7 +271,7 @@ workflow PROFILING { ch_input_for_krakenuniq = ch_input_for_profiling.krakenuniq .map { meta, reads, db_meta, db -> - [[single_end: meta.single_end], reads, db_meta, db] + [[id: db_meta.db_name, single_end: meta.single_end], reads, db_meta, db] } .groupTuple(by: [0,2,3]) .dump(tag: "krakenuniq_premultimap") From f389029848e0044568ecc92c8cb004e5ada69653 Mon Sep 17 00:00:00 2001 From: sofstam Date: Tue, 29 Nov 2022 14:38:39 +0100 Subject: [PATCH 111/132] Fix merge conflic --- nextflow_schema.json | 1475 +++++++++++++++++++++--------------------- 1 file changed, 741 insertions(+), 734 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 8e2250b..df4b4e7 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,749 +1,756 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/taxprofiler/master/nextflow_schema.json", - "title": "nf-core/taxprofiler pipeline parameters", - "description": "Taxonomic profiling of shotgun metagenomic data", - "type": "object", - "definitions": { - "input_output_options": { - "title": "Input/output options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "databases", "outdir"], - "properties": { - "input": { - "type": "string", - "format": "file-path", - "mimetype": "text/csv", - "pattern": "^\\S+\\.(csv|yaml|yml)$", - "schema": "assets/schema_input.json", - "description": "Path to comma-separated file containing information about the samples and libraries/runs.", - "help_text": "You will need to create a design file with information about the samples and libraries/runs you want to running in your pipeline run. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" - }, - "databases": { - "type": "string", - "mimetype": "text/csv", - "format": "file-path", - "fa_icon": "fas fa-database", - "description": "Path to comma-separated file containing information about databases and profiling parameters for each taxonomic profiler", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/dev/usage#full-database-sheet).\n\nProfilers will only be executed if a corresponding database are supplied. \n\nWe recommend storing this database sheet somewhere centrally and accessible by others members of your lab/institutions, as this file will likely be regularly reused." - }, - "outdir": { - "type": "string", - "format": "directory-path", - "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" - }, - "email": { - "type": "string", - "description": "Email address for completion summary.", - "fa_icon": "fas fa-envelope", - "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" - }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" - } - } + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/taxprofiler/master/nextflow_schema.json", + "title": "nf-core/taxprofiler pipeline parameters", + "description": "Taxonomic profiling of shotgun metagenomic data", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["input", "databases", "outdir"], + "properties": { + "input": { + "type": "string", + "format": "file-path", + "mimetype": "text/csv", + "pattern": "^\\S+\\.(csv|yaml|yml)$", + "schema": "assets/schema_input.json", + "description": "Path to comma-separated file containing information about the samples and libraries/runs.", + "help_text": "You will need to create a design file with information about the samples and libraries/runs you want to running in your pipeline run. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" }, - "preprocessing_general_qc_options": { - "title": "Preprocessing general QC options", - "type": "object", - "description": "Common options across both long and short read preprocessing QC steps", - "default": "", - "properties": { - "save_preprocessed_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save reads from adapter clipping/pair-merging, length filtering for both short and long reads", - "help_text": "This saves the FASTQ output from the following tools:\n\n- fastp\n- AdapterRemoval\n- Porechop\n- Filtlong\n\nThese reads will be a mixture of: adapter clipped, quality trimmed, pair-merged, and length filtered, depending on the parameters you set." - } - }, - "fa_icon": "fas fa-users-cog" + "databases": { + "type": "string", + "mimetype": "text/csv", + "format": "file-path", + "fa_icon": "fas fa-database", + "description": "Path to comma-separated file containing information about databases and profiling parameters for each taxonomic profiler", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/dev/usage#full-database-sheet).\n\nProfilers will only be executed if a corresponding database are supplied. \n\nWe recommend storing this database sheet somewhere centrally and accessible by others members of your lab/institutions, as this file will likely be regularly reused." }, - "preprocessing_short_read_qc_options": { - "title": "Preprocessing short-read QC options", - "type": "object", - "description": "Options for adapter clipping, quality trimming, pair-merging, and complexity filtering", - "default": "", - "properties": { - "perform_shortread_qc": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)", - "help_text": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)\n\nThis subworkflow can perform:\n\n- Adapter removal\n- Read quality trimming\n- Read pair merging\n- Length filtering\n- Complexity filtering\n\nEither with fastp or AdapterRemoval.\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences. Note that some, but not all, tools support paired-end alignment (utilising information about the insert covered by the pairs). However read pair merging in some cases can be recommend to increase read length (such as in aDNA). Length filtering, and/or complexity can speed up alignment by reducing the number of short unspecific reads that need to be aligned." - }, - "shortread_qc_tool": { - "type": "string", - "default": "fastp", - "enum": ["fastp", "adapterremoval"], - "fa_icon": "fas fa-tools", - "description": "Specify which tool to use for short-read QC" - }, - "shortread_qc_skipadaptertrim": { - "type": "boolean", - "fa_icon": "fas fa-forward", - "description": "Skip adapter trimming", - "help_text": "Skip the removal of sequencing adapters. \n\nThis often can be useful to speed up run-time of the pipeline when analysing data downloaded from public databases such as the ENA or SRA, as adapters should already be removed (however we recommend to check FastQC results to ensure this is the case)." - }, - "shortread_qc_adapter1": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-grip-lines", - "description": "Specify adapter 1 nucleotide sequence", - "help_text": "Specify a custom forward or R1 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG`" - }, - "shortread_qc_adapter2": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-grip-lines", - "description": "Specify adapter 2 nucleotide sequence", - "help_text": "Specify a custom reverse or R2 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT`" - }, - "shortread_qc_adapterlist": { - "type": "string", - "default": "None", - "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", - "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possible combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" - }, - "shortread_qc_mergepairs": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on merging of read pairs for paired-end data", - "default": true, - "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" - }, - "shortread_qc_excludeunmerged": { - "type": "boolean", - "fa_icon": "far fa-times-circle", - "description": "Discard unmerged reads from paired-end merging", - "help_text": "Turns off the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nThis can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - removed from reads `--include_unmerged`\n" - }, - "shortread_qc_minlength": { - "type": "integer", - "default": 15, - "fa_icon": "fas fa-ruler-horizontal", - "description": "Specify the minimum length of reads to be retained", - "help_text": "Specifying a mimum read length filtering can speed up profiling by reducing the number of short unspecific reads that need to be match/aligned to the database.\n\n> Modifies tool parameter(s):\n> - removed from reads `--length_required`\n> - AdapterRemoval: `--minlength`" - }, - "perform_shortread_complexityfilter": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on nucleotide sequence complexity filtering", - "help_text": "Turns on sequencing complexity filtering. Complexity filtering can be useful to increase run-time by removing unspecific read sequences that do not provide any informative taxon ID." - }, - "shortread_complexityfilter_tool": { - "type": "string", - "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], - "fa_icon": "fas fa-hammer", - "description": "Specify which tool to use for complexity filtering" - }, - "shortread_complexityfilter_entropy": { - "type": "number", - "default": 0.3, - "fa_icon": "fas fa-random", - "description": "Specify the minimum sequence entropy level for complexity filtering", - "help_text": "Specify the minimum 'entropy' value for complexity filtering for BBDuk or PRINSEQ++.\n\nNote that this value will only be used for PRINSEQ++ if `--shortread_complexityfilter_prinseqplusplus_mode` is set to `entropy`.\n\nEntropy here corresponds to the amount of sequence variation exists within the read. Higher values correspond to more variety, and thus will likely reslut in more specific matching to a taxon's reference genome. The trade off here is fewer reads (or abundance information) available for having a confident identification.\n\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropy=`\n> - PRINSEQ++: `-lc_entropy`\n\n" - }, - "shortread_complexityfilter_bbduk_windowsize": { - "type": "integer", - "default": 50, - "fa_icon": "far fa-window-maximize", - "description": "Specify the window size for BBDuk complexity filtering", - "help_text": "Specify the window size to calculate the level entropy within for BBDuk.\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropywindow=`" - }, - "shortread_complexityfilter_bbduk_mask": { - "type": "boolean", - "fa_icon": "fas fa-mask", - "description": "Turn on masking rather than discarding of low complexity reads for BBduk", - "help_text": "Turn on masking of low-complexity reads (i.e., replacement with `N`) rather than removal.\n\n> Modifies tool parameter(s)\n> - BBDuk: `entropymask=`" - }, - "shortread_complexityfilter_fastp_threshold": { - "type": "integer", - "default": 30, - "fa_icon": "fas fa-sort-numeric-down", - "description": "Specify the minimum complexity filter threshold of fastp", - "help_text": "Specify the minimum sequence complexity value for fastp. This value corresponds to the percentage of bases that is different from it's adjacent bases.\n\n> Modifies tool parameter(s):\n> - removed from reads `--complexity_threshold`" - }, - "shortread_complexityfilter_prinseqplusplus_mode": { - "type": "string", - "default": "entropy", - "enum": ["entropy", "dust"], - "fa_icon": "fas fa-check-square", - "description": "Specify the complexity filter mode for PRINSEQ++" - }, - "shortread_complexityfilter_prinseqplusplus_dustscore": { - "type": "number", - "default": 0.5, - "fa_icon": "fas fa-head-side-mask", - "description": "Specify the minimum dust score for PRINTSEQ++ complexity filtering", - "help_text": "Specify the minimum dust score below which low-complexity reads will be removed. A DUST score is based on how often different tri-nucleotides occur along a read.\n\n> Modifies tool parameter(s):\n> - PRINSEQ++: `--lc_dust`" - }, - "save_complexityfiltered_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save complexity filtered short-reads", - "help_text": "Specify whether to save the final complexity filtered reads in your results directory (`--outdir`)." - } - }, - "fa_icon": "fas fa-compress-alt" + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" }, - "preprocessing_long_read_qc_options": { - "title": "Preprocessing long-read QC options", - "type": "object", - "description": "Options for adapter clipping, quality trimming, and length filtering", - "default": "", - "properties": { - "perform_longread_qc": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on long read quality control steps (adapter clipping, length filtering etc.)", - "help_text": "Turns on long read quality control steps (adapter clipping, length and/or quality filtering.)\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences.\n\nLength filtering, and quality filtering can speed up alignment by reducing the number of unspecific reads that need to be aligned." - }, - "longread_qc_skipadaptertrim": { - "type": "boolean", - "description": "Skip long-read trimming", - "fa_icon": "fas fa-forward", - "help_text": "Skip removal of adapters by Porechop. This can be useful in some cases to speed up run time - particularly when you are running data downloading from public databases such as the ENA/SRA that should already have adapters removed. We recommend that you check your FastQC results this is indeed the case." - }, - "longread_qc_skipqualityfilter": { - "type": "boolean", - "description": "Skip long-read length and quality filtering", - "fa_icon": "fas fa-forward", - "help_text": "Skip removal of quality filtering with Filtlong. This will skip length, percent reads, and target bases filtering (see other `--longread_qc_qualityfilter_*` parameters)." - }, - "longread_qc_qualityfilter_minlength": { - "type": "integer", - "default": 1000, - "description": "Specify the minimum length of reads to be retained", - "fa_icon": "fas fa-ruler-horizontal", - "help_text": "Specify the minimum of length of reads to be kept for downstream analysis.\n\n> Modifies tool parameter(s):\n> - Filtlong: `--min_length`" - }, - "longread_qc_qualityfilter_keeppercent": { - "type": "integer", - "default": 90, - "description": "Specify the percent of high-quality bases to be retained", - "fa_icon": "fas fa-percentage", - "help_text": "Throw out the remaining percentage of reads outside the value. This is measured by bp, not by read count. So this option throws out the worst e.g. 10% of read bases if the parameter is set to `90`. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" - }, - "longread_qc_qualityfilter_targetbases": { - "type": "integer", - "default": 500000000, - "description": "Specify the number of high-quality bases in the library to be retained", - "fa_icon": "fas fa-bullseye", - "help_text": "Removes the worst reads until only the specified value of bases remain, useful for very large read sets. If the input read set is less than the specified value, this setting will have no effect. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" - } - }, - "fa_icon": "fas fa-expand-alt" + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" }, - "preprocessing_host_removal_options": { - "title": "Preprocessing host removal options", - "type": "object", - "description": "Options for pre-profiling host read removal", - "default": "", - "properties": { - "perform_shortread_hostremoval": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on short-read host removal", - "help_text": "Turns on the ability to remove short-reads from the that derived from a known organism, using Bowtie2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." - }, - "perform_longread_hostremoval": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on long-read host removal", - "help_text": "Turns on the ability to remove long-reads from the that derived from a known organism, using minimap2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." - }, - "hostremoval_reference": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-file-alt", - "description": "Specify path to single reference FASTA of host(s) genome(s)", - "help_text": "Specify a path to the FASTA file (optionally gzipped) of the reference genome of the organism to be removed.\n\nIf you have two or more host organisms or contaminants you wish to remove, you can concatenate the FASTAs of the different taxa into a single one to provide to the pipeline." - }, - "shortread_hostremoval_index": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-address-book", - "description": "Specify path to the directory containing pre-made BowTie2 indexes of the host removal reference", - "help_text": "Specify the path to a _directory_ containing pre-made Bowtie2 reference index files (i.e. the directory containing `.bt1`, `.bt2` files etc.). These should sit in the same directory alongside the the reference file specified in `--hostremoval_reference`.\n\nSpecifying premade indices can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." - }, - "longread_hostremoval_index": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-address-book", - "description": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference", - "help_text": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference file given to `--hostremoval_reference`.\n\nSpecifying a premade index file can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." - }, - "save_hostremoval_index": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save mapping index of input reference when not already supplied by user", - "help_text": "Save the output files of the in-built indexing of the host genome.\n\nThis is recommend to be turned on if you plan to use the same reference genome multiple times, as supplying the directory or file to `--shortread_hostremoval_index` or `--longread_hostremoval_index` respectively can speed up runtime of future runs. Once generated, we recommend you place this file _outside_ of your run results directory in a central 'cache' directory you and others using your machine can access and supply to the pipeline." - }, - "save_hostremoval_mapped": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save mapped reads in BAM format from host removal", - "help_text": "Save the reads mapped to the reference genome in BAM format as output by the respective hostremoval alignment tool.\n\nThis can be useful if you wish to perform other analyses on the host organism (such as host-microbe interaction), however, you should consider whether the default mapping parameters of Bowtie2 (short-read) or minimap2 (long-read) are optimised to your context. " - }, - "save_hostremoval_unmapped": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save unmapped reads in FASTQ format from host removal", - "help_text": "Save the unreads mapped to the reference genome in FASTQ format (as exported from `samtools view`).\n\nThis can be useful if you wish to perform other analyses on the off-target reads from the host mapping, such as manual profiling or _de novo_ assembly." - } - }, - "fa_icon": "fas fa-user-times" - }, - "preprocessing_run_merging_options": { - "title": "Preprocessing run merging options", - "type": "object", - "description": "Options for per-sample run-merging", - "default": "", - "properties": { - "perform_runmerging": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on run merging", - "help_text": "Turns on the concatenation of sequencing runs or libraries with the same sample name.\n\nThis can be useful to ensure you get a single profile per sample, rather than one profile per run or library. Note that in some cases comparing profiles of independent _libraries_ may be useful, so this parameter may not always be suitable. " - }, - "save_runmerged_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save run-concatenated input FASTQ files for each sample", - "help_text": "Save the run- and library-concatenated reads of a given sample in FASTQ format." - } - }, - "fa_icon": "fas fa-clipboard-check" - }, - "profiling_options": { - "title": "Profiling options", - "type": "object", - "description": "", - "default": "", - "properties": { - "run_centrifuge": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Centrifuge. Requires database to be present CSV file passed to --databases" - }, - "centrifuge_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Centrifuge-aligned reads", - "help_text": "Save mapped (SAM, FASTQ) and unmapped (FASTQ) reads from alignment step of centrifuge in your output results directory.\n\n> Modifies tool parameter(s):\n> - centrifuge: `--un-gz`, `--al-gz`, `--un-conc-gz`, `--al-conc-gz`, `--out-fmt`" - }, - "run_diamond": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with DIAMOND. Requires database to be present CSV file passed to --databases" - }, - "diamond_output_format": { - "type": "string", - "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], - "fa_icon": "fas fa-file", - "description": "Specify output format from DIAMOND profiling.", - "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" - }, - "diamond_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of DIAMOND-aligned reads. Will override --diamond_output_format and no taxon tables will be generated", - "help_text": "Save aligned reads in SAM format from alignment step of DIAMOND in your output results directory.\n\nNote this explicitly overrides `--diamond_output_format` to produce the SAM file, and no taxon table will be generated.\n\n> Modifies tool parameter(s):\n> - DIAMOND: `--outfmt`" - }, - "run_kaiju": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Kaiju. Requires database to be present CSV file passed to --databases" - }, - "kaiju_taxon_rank": { - "type": "string", - "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], - "fa_icon": "fas fa-tag", - "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", - "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" - }, - "run_kraken2": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Kraken2. Requires database to be present CSV file passed to --databases" - }, - "kraken2_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Kraken2-aligned reads", - "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - kraken2: `--classified-out` and `--unclassified-out`" - }, - "kraken2_save_readclassification": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Kraken2 per-read taxonomic assignment file", - "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - kraken2: `--output`" - }, - "kraken2_save_minimizers": { - "type": "boolean", - "description": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.", - "fa_icon": "fas fa-save", - "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." - }, - "run_bracken": { - "type": "boolean", - "description": "Post-process kraken2 reports with Bracken.", - "fa_icon": "fas fa-toggle-on" - }, - "run_malt": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with MALT. Requires database to be present CSV file passed to --databases" - }, - "malt_mode": { - "type": "string", - "default": "BlastN", - "fa_icon": "fas fa-check-square", - "description": "Specify which MALT alignment mode to use", - "help_text": "Specify which version of MALT alignment to use.\n\nBlastN is generally recommended (nucleotide-nucleotide alignment), but particularly for very short reads (such as aDNA), whereas BlastX mode is similar to DIAMOND and will translate the nucleotide to amino acid sequences. Note each type of alignment mode requires different parameters during database construction. Refer to the MALT manual for more information.\n\n> Modifies tool parameter(s):\n> - malt-run: `-mode` " - }, - "malt_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of MALT-aligned reads", - "help_text": "Turns on saving of MALT aligned reads in SAM format.\n\nNote that the SAM format produce by MALT is not completely valid, and may not work with downstream tools.\n\n> Modifies tool parameter(s):\n> - malt-run: `--alignments`, `-za`" - }, - "malt_generate_megansummary": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on generation of MEGAN summary file from MALT results", - "help_text": "Turns on saving of MALT output in an additional MEGAN summary file (`.megan`) that can be loaded into the MEGAN metagenomic exploration tool.\n\nNote: this file is generated not directly from MALT but rather then MEGAN utility script `rma2info`.\n\n> Modifies tool parameter(s):\n> - rma2info: `-es`" - }, - "run_metaphlan3": { - "type": "boolean", - "description": "Turn on profiling with MetaPhlAn3. Requires database to be present CSV file passed to --databases", - "fa_icon": "fas fa-toggle-on" - }, - "run_motus": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" - } - }, - "fa_icon": "fas fa-align-center" - }, - "postprocessing_and_visualisation_options": { - "title": "Postprocessing and visualisation options", - "type": "object", - "description": "", - "default": "", - "properties": { - "run_profile_standardisation": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on standardisation of taxon tables across profilers", - "help_text": "Turns on standardisation of output OTU tables across all tools; each into a TSV format following the following scheme:\n\n|TAXON | SAMPLE_A | SAMPLE_B |\n|-------------|----------------|-----------------|\n| taxon_a | 32 | 123 |\n| taxon_b | 1 | 5 |\n\nThis currently only is generated for mOTUs." - }, - "generate_biom_output": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on generation of BIOM output (currently only applies to mOTUs)", - "help_text": "Turn on the saving of the taxonomic output in BIOM format (`.biom`) in the results directory of your pipeline run, instead of the default TSV format.\n\nNote this file is from the output of the `motus merge` command.\n\n> Modifies tool parameter(s):\n> - `-B -o`" - }, - "run_krona": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on generation of Krona plots for supported profilers", - "help_text": "Turn on the generation of Krona interactive pie-chart HTMLs for a selection of profilers.\n\nThe tools currently supported are:\n\n- centrifuge\n- kraken2\n- kaiju\n- MALT" - }, - "krona_taxonomy_directory": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-folder-open", - "description": "Specify path to krona taxonomy directories (required for MALT krona plots)", - "help_text": "Specify a path to a Krona taxonomy database directory (i.e. a directory containing a krona generated `.tab` file).\n\nThis is only required for generating Krona plots of MALT output.\n\nNote this taxonomy database must be downloaded and generated with the `updateTaxonomy.sh` script from the krona-tools package." - } - }, - "fa_icon": "fas fa-chart-line" - }, - "institutional_config_options": { - "title": "Institutional config options", - "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", - "properties": { - "custom_config_version": { - "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "custom_config_base": { - "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", - "fa_icon": "fas fa-users-cog" - }, - "config_profile_name": { - "type": "string", - "description": "Institutional config name.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_description": { - "type": "string", - "description": "Institutional config description.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - } - } - }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } - }, - "generic_options": { - "title": "Generic options", - "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", - "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, - "publish_dir_mode": { - "type": "string", - "default": "copy", - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], - "hidden": true - }, - "email_on_fail": { - "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", - "hidden": true - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", - "hidden": true - }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true - }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", - "hidden": true - }, - "hook_url": { - "type": "string", - "description": "Incoming hook URL for messaging service", - "fa_icon": "fas fa-people-group", - "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", - "hidden": true - }, - "multiqc_config": { - "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "multiqc_logo": { - "type": "string", - "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", - "fa_icon": "fas fa-image", - "hidden": true - }, - "multiqc_methods_description": { - "type": "string", - "description": "Custom MultiQC yaml file containing HTML including a methods description.", - "fa_icon": "fas fa-cog" - }, - "tracedir": { - "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "show_hidden_params": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "enable_conda": { - "type": "boolean", - "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", - "hidden": true, - "fa_icon": "fas fa-bacon" - } - } - }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", - "hidden": true - }, - "igenomes_base": { - "type": "string", - "format": "directory-path", - "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes", - "fa_icon": "fas fa-cloud-download-alt", - "hidden": true - }, - "igenomes_ignore": { - "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." - } - } + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" } + } }, - "allOf": [ - { - "$ref": "#/definitions/input_output_options" - }, - { - "$ref": "#/definitions/preprocessing_general_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_short_read_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_long_read_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_host_removal_options" - }, - { - "$ref": "#/definitions/preprocessing_run_merging_options" - }, - { - "$ref": "#/definitions/profiling_options" - }, - { - "$ref": "#/definitions/postprocessing_and_visualisation_options" - }, - { - "$ref": "#/definitions/institutional_config_options" - }, - { - "$ref": "#/definitions/max_job_request_options" - }, - { - "$ref": "#/definitions/generic_options" - }, - { - "$ref": "#/definitions/reference_genome_options" + "preprocessing_general_qc_options": { + "title": "Preprocessing general QC options", + "type": "object", + "description": "Common options across both long and short read preprocessing QC steps", + "default": "", + "properties": { + "save_preprocessed_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save reads from adapter clipping/pair-merging, length filtering for both short and long reads", + "help_text": "This saves the FASTQ output from the following tools:\n\n- fastp\n- AdapterRemoval\n- Porechop\n- Filtlong\n\nThese reads will be a mixture of: adapter clipped, quality trimmed, pair-merged, and length filtered, depending on the parameters you set." } - ], - "properties": { + }, + "fa_icon": "fas fa-users-cog" + }, + "preprocessing_short_read_qc_options": { + "title": "Preprocessing short-read QC options", + "type": "object", + "description": "Options for adapter clipping, quality trimming, pair-merging, and complexity filtering", + "default": "", + "properties": { + "perform_shortread_qc": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)", + "help_text": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)\n\nThis subworkflow can perform:\n\n- Adapter removal\n- Read quality trimming\n- Read pair merging\n- Length filtering\n- Complexity filtering\n\nEither with fastp or AdapterRemoval.\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences. Note that some, but not all, tools support paired-end alignment (utilising information about the insert covered by the pairs). However read pair merging in some cases can be recommend to increase read length (such as in aDNA). Length filtering, and/or complexity can speed up alignment by reducing the number of short unspecific reads that need to be aligned." + }, + "shortread_qc_tool": { + "type": "string", + "default": "fastp", + "enum": ["fastp", "adapterremoval"], + "fa_icon": "fas fa-tools", + "description": "Specify which tool to use for short-read QC" + }, + "shortread_qc_skipadaptertrim": { + "type": "boolean", + "fa_icon": "fas fa-forward", + "description": "Skip adapter trimming", + "help_text": "Skip the removal of sequencing adapters. \n\nThis often can be useful to speed up run-time of the pipeline when analysing data downloaded from public databases such as the ENA or SRA, as adapters should already be removed (however we recommend to check FastQC results to ensure this is the case)." + }, + "shortread_qc_adapter1": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-grip-lines", + "description": "Specify adapter 1 nucleotide sequence", + "help_text": "Specify a custom forward or R1 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG`" + }, + "shortread_qc_adapter2": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-grip-lines", + "description": "Specify adapter 2 nucleotide sequence", + "help_text": "Specify a custom reverse or R2 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT`" + }, + "shortread_qc_adapterlist": { + "type": "string", + "default": "None", + "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", + "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possible combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" + }, + "shortread_qc_mergepairs": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on merging of read pairs for paired-end data", + "default": true, + "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" + }, + "shortread_qc_excludeunmerged": { + "type": "boolean", + "fa_icon": "far fa-times-circle", + "description": "Discard unmerged reads from paired-end merging", + "help_text": "Turns off the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nThis can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - removed from reads `--include_unmerged`\n" + }, + "shortread_qc_minlength": { + "type": "integer", + "default": 15, + "fa_icon": "fas fa-ruler-horizontal", + "description": "Specify the minimum length of reads to be retained", + "help_text": "Specifying a mimum read length filtering can speed up profiling by reducing the number of short unspecific reads that need to be match/aligned to the database.\n\n> Modifies tool parameter(s):\n> - removed from reads `--length_required`\n> - AdapterRemoval: `--minlength`" + }, + "perform_shortread_complexityfilter": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on nucleotide sequence complexity filtering", + "help_text": "Turns on sequencing complexity filtering. Complexity filtering can be useful to increase run-time by removing unspecific read sequences that do not provide any informative taxon ID." + }, + "shortread_complexityfilter_tool": { + "type": "string", + "default": "bbduk", + "enum": ["bbduk", "prinseqplusplus", "fastp"], + "fa_icon": "fas fa-hammer", + "description": "Specify which tool to use for complexity filtering" + }, + "shortread_complexityfilter_entropy": { + "type": "number", + "default": 0.3, + "fa_icon": "fas fa-random", + "description": "Specify the minimum sequence entropy level for complexity filtering", + "help_text": "Specify the minimum 'entropy' value for complexity filtering for BBDuk or PRINSEQ++.\n\nNote that this value will only be used for PRINSEQ++ if `--shortread_complexityfilter_prinseqplusplus_mode` is set to `entropy`.\n\nEntropy here corresponds to the amount of sequence variation exists within the read. Higher values correspond to more variety, and thus will likely reslut in more specific matching to a taxon's reference genome. The trade off here is fewer reads (or abundance information) available for having a confident identification.\n\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropy=`\n> - PRINSEQ++: `-lc_entropy`\n\n" + }, + "shortread_complexityfilter_bbduk_windowsize": { + "type": "integer", + "default": 50, + "fa_icon": "far fa-window-maximize", + "description": "Specify the window size for BBDuk complexity filtering", + "help_text": "Specify the window size to calculate the level entropy within for BBDuk.\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropywindow=`" + }, + "shortread_complexityfilter_bbduk_mask": { + "type": "boolean", + "fa_icon": "fas fa-mask", + "description": "Turn on masking rather than discarding of low complexity reads for BBduk", + "help_text": "Turn on masking of low-complexity reads (i.e., replacement with `N`) rather than removal.\n\n> Modifies tool parameter(s)\n> - BBDuk: `entropymask=`" + }, + "shortread_complexityfilter_fastp_threshold": { + "type": "integer", + "default": 30, + "fa_icon": "fas fa-sort-numeric-down", + "description": "Specify the minimum complexity filter threshold of fastp", + "help_text": "Specify the minimum sequence complexity value for fastp. This value corresponds to the percentage of bases that is different from it's adjacent bases.\n\n> Modifies tool parameter(s):\n> - removed from reads `--complexity_threshold`" + }, + "shortread_complexityfilter_prinseqplusplus_mode": { + "type": "string", + "default": "entropy", + "enum": ["entropy", "dust"], + "fa_icon": "fas fa-check-square", + "description": "Specify the complexity filter mode for PRINSEQ++" + }, + "shortread_complexityfilter_prinseqplusplus_dustscore": { + "type": "number", + "default": 0.5, + "fa_icon": "fas fa-head-side-mask", + "description": "Specify the minimum dust score for PRINTSEQ++ complexity filtering", + "help_text": "Specify the minimum dust score below which low-complexity reads will be removed. A DUST score is based on how often different tri-nucleotides occur along a read.\n\n> Modifies tool parameter(s):\n> - PRINSEQ++: `--lc_dust`" + }, + "save_complexityfiltered_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save complexity filtered short-reads", + "help_text": "Specify whether to save the final complexity filtered reads in your results directory (`--outdir`)." + } + }, + "fa_icon": "fas fa-compress-alt" + }, + "preprocessing_long_read_qc_options": { + "title": "Preprocessing long-read QC options", + "type": "object", + "description": "Options for adapter clipping, quality trimming, and length filtering", + "default": "", + "properties": { + "perform_longread_qc": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on long read quality control steps (adapter clipping, length filtering etc.)", + "help_text": "Turns on long read quality control steps (adapter clipping, length and/or quality filtering.)\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences.\n\nLength filtering, and quality filtering can speed up alignment by reducing the number of unspecific reads that need to be aligned." + }, + "longread_qc_skipadaptertrim": { + "type": "boolean", + "description": "Skip long-read trimming", + "fa_icon": "fas fa-forward", + "help_text": "Skip removal of adapters by Porechop. This can be useful in some cases to speed up run time - particularly when you are running data downloading from public databases such as the ENA/SRA that should already have adapters removed. We recommend that you check your FastQC results this is indeed the case." + }, + "longread_qc_skipqualityfilter": { + "type": "boolean", + "description": "Skip long-read length and quality filtering", + "fa_icon": "fas fa-forward", + "help_text": "Skip removal of quality filtering with Filtlong. This will skip length, percent reads, and target bases filtering (see other `--longread_qc_qualityfilter_*` parameters)." + }, + "longread_qc_qualityfilter_minlength": { + "type": "integer", + "default": 1000, + "description": "Specify the minimum length of reads to be retained", + "fa_icon": "fas fa-ruler-horizontal", + "help_text": "Specify the minimum of length of reads to be kept for downstream analysis.\n\n> Modifies tool parameter(s):\n> - Filtlong: `--min_length`" + }, + "longread_qc_qualityfilter_keeppercent": { + "type": "integer", + "default": 90, + "description": "Specify the percent of high-quality bases to be retained", + "fa_icon": "fas fa-percentage", + "help_text": "Throw out the remaining percentage of reads outside the value. This is measured by bp, not by read count. So this option throws out the worst e.g. 10% of read bases if the parameter is set to `90`. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" + }, + "longread_qc_qualityfilter_targetbases": { + "type": "integer", + "default": 500000000, + "description": "Specify the number of high-quality bases in the library to be retained", + "fa_icon": "fas fa-bullseye", + "help_text": "Removes the worst reads until only the specified value of bases remain, useful for very large read sets. If the input read set is less than the specified value, this setting will have no effect. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" + } + }, + "fa_icon": "fas fa-expand-alt" + }, + "preprocessing_host_removal_options": { + "title": "Preprocessing host removal options", + "type": "object", + "description": "Options for pre-profiling host read removal", + "default": "", + "properties": { + "perform_shortread_hostremoval": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on short-read host removal", + "help_text": "Turns on the ability to remove short-reads from the that derived from a known organism, using Bowtie2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." + }, + "perform_longread_hostremoval": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on long-read host removal", + "help_text": "Turns on the ability to remove long-reads from the that derived from a known organism, using minimap2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." + }, + "hostremoval_reference": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-file-alt", + "description": "Specify path to single reference FASTA of host(s) genome(s)", + "help_text": "Specify a path to the FASTA file (optionally gzipped) of the reference genome of the organism to be removed.\n\nIf you have two or more host organisms or contaminants you wish to remove, you can concatenate the FASTAs of the different taxa into a single one to provide to the pipeline." + }, + "shortread_hostremoval_index": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-address-book", + "description": "Specify path to the directory containing pre-made BowTie2 indexes of the host removal reference", + "help_text": "Specify the path to a _directory_ containing pre-made Bowtie2 reference index files (i.e. the directory containing `.bt1`, `.bt2` files etc.). These should sit in the same directory alongside the the reference file specified in `--hostremoval_reference`.\n\nSpecifying premade indices can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." + }, + "longread_hostremoval_index": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-address-book", + "description": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference", + "help_text": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference file given to `--hostremoval_reference`.\n\nSpecifying a premade index file can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." + }, + "save_hostremoval_index": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save mapping index of input reference when not already supplied by user", + "help_text": "Save the output files of the in-built indexing of the host genome.\n\nThis is recommend to be turned on if you plan to use the same reference genome multiple times, as supplying the directory or file to `--shortread_hostremoval_index` or `--longread_hostremoval_index` respectively can speed up runtime of future runs. Once generated, we recommend you place this file _outside_ of your run results directory in a central 'cache' directory you and others using your machine can access and supply to the pipeline." + }, + "save_hostremoval_mapped": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save mapped reads in BAM format from host removal", + "help_text": "Save the reads mapped to the reference genome in BAM format as output by the respective hostremoval alignment tool.\n\nThis can be useful if you wish to perform other analyses on the host organism (such as host-microbe interaction), however, you should consider whether the default mapping parameters of Bowtie2 (short-read) or minimap2 (long-read) are optimised to your context. " + }, + "save_hostremoval_unmapped": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save unmapped reads in FASTQ format from host removal", + "help_text": "Save the unreads mapped to the reference genome in FASTQ format (as exported from `samtools view`).\n\nThis can be useful if you wish to perform other analyses on the off-target reads from the host mapping, such as manual profiling or _de novo_ assembly." + } + }, + "fa_icon": "fas fa-user-times" + }, + "preprocessing_run_merging_options": { + "title": "Preprocessing run merging options", + "type": "object", + "description": "Options for per-sample run-merging", + "default": "", + "properties": { + "perform_runmerging": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on run merging", + "help_text": "Turns on the concatenation of sequencing runs or libraries with the same sample name.\n\nThis can be useful to ensure you get a single profile per sample, rather than one profile per run or library. Note that in some cases comparing profiles of independent _libraries_ may be useful, so this parameter may not always be suitable. " + }, + "save_runmerged_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save run-concatenated input FASTQ files for each sample", + "help_text": "Save the run- and library-concatenated reads of a given sample in FASTQ format." + } + }, + "fa_icon": "fas fa-clipboard-check" + }, + "profiling_options": { + "title": "Profiling options", + "type": "object", + "description": "", + "default": "", + "properties": { + "run_centrifuge": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Centrifuge. Requires database to be present CSV file passed to --databases" + }, + "centrifuge_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Centrifuge-aligned reads", + "help_text": "Save mapped (SAM, FASTQ) and unmapped (FASTQ) reads from alignment step of centrifuge in your output results directory.\n\n> Modifies tool parameter(s):\n> - centrifuge: `--un-gz`, `--al-gz`, `--un-conc-gz`, `--al-conc-gz`, `--out-fmt`" + }, + "run_diamond": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with DIAMOND. Requires database to be present CSV file passed to --databases" + }, + "diamond_output_format": { + "type": "string", + "default": "tsv", + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "fa_icon": "fas fa-file", + "description": "Specify output format from DIAMOND profiling.", + "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" + }, + "diamond_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of DIAMOND-aligned reads. Will override --diamond_output_format and no taxon tables will be generated", + "help_text": "Save aligned reads in SAM format from alignment step of DIAMOND in your output results directory.\n\nNote this explicitly overrides `--diamond_output_format` to produce the SAM file, and no taxon table will be generated.\n\n> Modifies tool parameter(s):\n> - DIAMOND: `--outfmt`" + }, + "run_kaiju": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Kaiju. Requires database to be present CSV file passed to --databases" + }, + "kaiju_taxon_rank": { + "type": "string", + "default": "species", + "enum": ["phylum", "class", "order", "family", "genus", "species"], + "fa_icon": "fas fa-tag", + "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", + "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" + }, + "run_kraken2": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Kraken2. Requires database to be present CSV file passed to --databases" + }, + "kraken2_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Kraken2-aligned reads", + "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - kraken2: `--classified-out` and `--unclassified-out`" + }, + "kraken2_save_readclassification": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Kraken2 per-read taxonomic assignment file", + "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - kraken2: `--output`" + }, + "kraken2_save_minimizers": { + "type": "boolean", + "description": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.", + "fa_icon": "fas fa-save", + "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." + }, + "run_bracken": { + "type": "boolean", + "description": "Post-process kraken2 reports with Bracken.", + "fa_icon": "fas fa-toggle-on" + }, + "run_malt": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with MALT. Requires database to be present CSV file passed to --databases" + }, + "malt_mode": { + "type": "string", + "default": "BlastN", + "fa_icon": "fas fa-check-square", + "description": "Specify which MALT alignment mode to use", + "help_text": "Specify which version of MALT alignment to use.\n\nBlastN is generally recommended (nucleotide-nucleotide alignment), but particularly for very short reads (such as aDNA), whereas BlastX mode is similar to DIAMOND and will translate the nucleotide to amino acid sequences. Note each type of alignment mode requires different parameters during database construction. Refer to the MALT manual for more information.\n\n> Modifies tool parameter(s):\n> - malt-run: `-mode` " + }, + "malt_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of MALT-aligned reads", + "help_text": "Turns on saving of MALT aligned reads in SAM format.\n\nNote that the SAM format produce by MALT is not completely valid, and may not work with downstream tools.\n\n> Modifies tool parameter(s):\n> - malt-run: `--alignments`, `-za`" + }, + "malt_generate_megansummary": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on generation of MEGAN summary file from MALT results", + "help_text": "Turns on saving of MALT output in an additional MEGAN summary file (`.megan`) that can be loaded into the MEGAN metagenomic exploration tool.\n\nNote: this file is generated not directly from MALT but rather then MEGAN utility script `rma2info`.\n\n> Modifies tool parameter(s):\n> - rma2info: `-es`" + }, + "run_metaphlan3": { + "type": "boolean", + "description": "Turn on profiling with MetaPhlAn3. Requires database to be present CSV file passed to --databases", + "fa_icon": "fas fa-toggle-on" + }, + "run_motus": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" + } + }, + "fa_icon": "fas fa-align-center" + }, + "postprocessing_and_visualisation_options": { + "title": "Postprocessing and visualisation options", + "type": "object", + "description": "", + "default": "", + "properties": { + "run_profile_standardisation": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on standardisation of taxon tables across profilers", + "help_text": "Turns on standardisation of output OTU tables across all tools; each into a TSV format following the following scheme:\n\n|TAXON | SAMPLE_A | SAMPLE_B |\n|-------------|----------------|-----------------|\n| taxon_a | 32 | 123 |\n| taxon_b | 1 | 5 |\n\nThis currently only is generated for mOTUs." + }, + "generate_biom_output": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on generation of BIOM output (currently only applies to mOTUs)", + "help_text": "Turn on the saving of the taxonomic output in BIOM format (`.biom`) in the results directory of your pipeline run, instead of the default TSV format.\n\nNote this file is from the output of the `motus merge` command.\n\n> Modifies tool parameter(s):\n> - `-B -o`" + }, + "run_krona": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on generation of Krona plots for supported profilers", + "help_text": "Turn on the generation of Krona interactive pie-chart HTMLs for a selection of profilers.\n\nThe tools currently supported are:\n\n- centrifuge\n- kraken2\n- kaiju\n- MALT" + }, + "krona_taxonomy_directory": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-folder-open", + "description": "Specify path to krona taxonomy directories (required for MALT krona plots)", + "help_text": "Specify a path to a Krona taxonomy database directory (i.e. a directory containing a krona generated `.tab` file).\n\nThis is only required for generating Krona plots of MALT output.\n\nNote this taxonomy database must be downloaded and generated with the `updateTaxonomy.sh` script from the krona-tools package." + } + }, + "fa_icon": "fas fa-chart-line" + }, + "institutional_config_options": { + "title": "Institutional config options", + "type": "object", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "properties": { + "custom_config_version": { + "type": "string", + "description": "Git commit id for Institutional configs.", + "default": "master", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "custom_config_base": { + "type": "string", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", + "hidden": true, + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" + }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], + "hidden": true + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, "preprocessing_qc_tool": { - "type": "string", - "default": "fastqc", - "enum": ["fastqc", "falco"], - "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", - "description": "Specify the tool used for quality control of raw sequencing reads" + "type": "string", + "default": "fastqc", + "enum": ["fastqc", "falco"], + "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", + "description": "Specify the tool used for quality control of raw sequencing reads" }, - "save_mgc_counts": { - "type": "boolean", - "description": "Save the mgc reads count for mOTUs." + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true }, - "save_ncbi_id": { - "type": "boolean", - "description": "Print NCBI taxonomic id for mOTUs." + "hook_url": { + "type": "string", + "description": "Incoming hook URL for messaging service", + "fa_icon": "fas fa-people-group", + "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", + "hidden": true }, - "save_read_counts": { - "type": "boolean", - "description": "Print mOTUs result as counts instead of relative abundances." + "multiqc_config": { + "type": "string", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_logo": { + "type": "string", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", + "hidden": true + }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, + "tracedir": { + "type": "string", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", + "hidden": true, + "fa_icon": "fas fa-bacon" } + } + }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Reference genome related files and options required for the workflow.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", + "hidden": true + }, + "igenomes_base": { + "type": "string", + "format": "directory-path", + "description": "Directory / URL base for iGenomes references.", + "default": "s3://ngi-igenomes/igenomes", + "fa_icon": "fas fa-cloud-download-alt", + "hidden": true + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/preprocessing_general_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_short_read_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_long_read_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_host_removal_options" + }, + { + "$ref": "#/definitions/preprocessing_run_merging_options" + }, + { + "$ref": "#/definitions/profiling_options" + }, + { + "$ref": "#/definitions/postprocessing_and_visualisation_options" + }, + { + "$ref": "#/definitions/institutional_config_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/generic_options" + }, + { + "$ref": "#/definitions/reference_genome_options" + } + ], + "properties": { + "save_mgc_counts": { + "type": "boolean", + "description": "Save the mgc reads count for mOTUs." + }, + "save_ncbi_id": { + "type": "boolean", + "description": "Print NCBI taxonomic id for mOTUs." + }, + "save_read_counts": { + "type": "boolean", + "description": "Print mOTUs result as counts instead of relative abundances." + } + } } From 12dd836e9c3f99d1028d43d6745ae66f48b6a2f4 Mon Sep 17 00:00:00 2001 From: sofstam Date: Tue, 29 Nov 2022 15:00:27 +0100 Subject: [PATCH 112/132] Fix conflict --- nextflow_schema.json | 1526 +++++++++++++++++++++--------------------- 1 file changed, 778 insertions(+), 748 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index df4b4e7..0ec5d48 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,756 +1,786 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/taxprofiler/master/nextflow_schema.json", - "title": "nf-core/taxprofiler pipeline parameters", - "description": "Taxonomic profiling of shotgun metagenomic data", - "type": "object", - "definitions": { - "input_output_options": { - "title": "Input/output options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "databases", "outdir"], - "properties": { - "input": { - "type": "string", - "format": "file-path", - "mimetype": "text/csv", - "pattern": "^\\S+\\.(csv|yaml|yml)$", - "schema": "assets/schema_input.json", - "description": "Path to comma-separated file containing information about the samples and libraries/runs.", - "help_text": "You will need to create a design file with information about the samples and libraries/runs you want to running in your pipeline run. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/taxprofiler/master/nextflow_schema.json", + "title": "nf-core/taxprofiler pipeline parameters", + "description": "Taxonomic profiling of shotgun metagenomic data", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": [ + "input", + "databases", + "outdir" + ], + "properties": { + "input": { + "type": "string", + "format": "file-path", + "mimetype": "text/csv", + "pattern": "^\\S+\\.(csv|yaml|yml)$", + "schema": "assets/schema_input.json", + "description": "Path to comma-separated file containing information about the samples and libraries/runs.", + "help_text": "You will need to create a design file with information about the samples and libraries/runs you want to running in your pipeline run. Use this parameter to specify its location. It has to be a comma-separated file with 6 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" + }, + "databases": { + "type": "string", + "mimetype": "text/csv", + "format": "file-path", + "fa_icon": "fas fa-database", + "description": "Path to comma-separated file containing information about databases and profiling parameters for each taxonomic profiler", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/dev/usage#full-database-sheet).\n\nProfilers will only be executed if a corresponding database are supplied. \n\nWe recommend storing this database sheet somewhere centrally and accessible by others members of your lab/institutions, as this file will likely be regularly reused." + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" + } + } }, - "databases": { - "type": "string", - "mimetype": "text/csv", - "format": "file-path", - "fa_icon": "fas fa-database", - "description": "Path to comma-separated file containing information about databases and profiling parameters for each taxonomic profiler", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row. See [usage docs](https://nf-co.re/taxprofiler/dev/usage#full-database-sheet).\n\nProfilers will only be executed if a corresponding database are supplied. \n\nWe recommend storing this database sheet somewhere centrally and accessible by others members of your lab/institutions, as this file will likely be regularly reused." + "preprocessing_general_qc_options": { + "title": "Preprocessing general QC options", + "type": "object", + "description": "Common options across both long and short read preprocessing QC steps", + "default": "", + "properties": { + "save_preprocessed_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save reads from adapter clipping/pair-merging, length filtering for both short and long reads", + "help_text": "This saves the FASTQ output from the following tools:\n\n- fastp\n- AdapterRemoval\n- Porechop\n- Filtlong\n\nThese reads will be a mixture of: adapter clipped, quality trimmed, pair-merged, and length filtered, depending on the parameters you set." + } + }, + "fa_icon": "fas fa-users-cog" }, - "outdir": { - "type": "string", - "format": "directory-path", - "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" + "preprocessing_short_read_qc_options": { + "title": "Preprocessing short-read QC options", + "type": "object", + "description": "Options for adapter clipping, quality trimming, pair-merging, and complexity filtering", + "default": "", + "properties": { + "perform_shortread_qc": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)", + "help_text": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)\n\nThis subworkflow can perform:\n\n- Adapter removal\n- Read quality trimming\n- Read pair merging\n- Length filtering\n- Complexity filtering\n\nEither with fastp or AdapterRemoval.\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences. Note that some, but not all, tools support paired-end alignment (utilising information about the insert covered by the pairs). However read pair merging in some cases can be recommend to increase read length (such as in aDNA). Length filtering, and/or complexity can speed up alignment by reducing the number of short unspecific reads that need to be aligned." + }, + "shortread_qc_tool": { + "type": "string", + "default": "fastp", + "enum": [ + "fastp", + "adapterremoval" + ], + "fa_icon": "fas fa-tools", + "description": "Specify which tool to use for short-read QC" + }, + "shortread_qc_skipadaptertrim": { + "type": "boolean", + "fa_icon": "fas fa-forward", + "description": "Skip adapter trimming", + "help_text": "Skip the removal of sequencing adapters. \n\nThis often can be useful to speed up run-time of the pipeline when analysing data downloaded from public databases such as the ENA or SRA, as adapters should already be removed (however we recommend to check FastQC results to ensure this is the case)." + }, + "shortread_qc_adapter1": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-grip-lines", + "description": "Specify adapter 1 nucleotide sequence", + "help_text": "Specify a custom forward or R1 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG`" + }, + "shortread_qc_adapter2": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-grip-lines", + "description": "Specify adapter 2 nucleotide sequence", + "help_text": "Specify a custom reverse or R2 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT`" + }, + "shortread_qc_adapterlist": { + "type": "string", + "default": "None", + "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", + "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possible combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" + }, + "shortread_qc_mergepairs": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on merging of read pairs for paired-end data", + "default": true, + "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" + }, + "shortread_qc_excludeunmerged": { + "type": "boolean", + "fa_icon": "far fa-times-circle", + "description": "Discard unmerged reads from paired-end merging", + "help_text": "Turns off the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nThis can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - removed from reads `--include_unmerged`\n" + }, + "shortread_qc_minlength": { + "type": "integer", + "default": 15, + "fa_icon": "fas fa-ruler-horizontal", + "description": "Specify the minimum length of reads to be retained", + "help_text": "Specifying a mimum read length filtering can speed up profiling by reducing the number of short unspecific reads that need to be match/aligned to the database.\n\n> Modifies tool parameter(s):\n> - removed from reads `--length_required`\n> - AdapterRemoval: `--minlength`" + }, + "perform_shortread_complexityfilter": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on nucleotide sequence complexity filtering", + "help_text": "Turns on sequencing complexity filtering. Complexity filtering can be useful to increase run-time by removing unspecific read sequences that do not provide any informative taxon ID." + }, + "shortread_complexityfilter_tool": { + "type": "string", + "default": "bbduk", + "enum": [ + "bbduk", + "prinseqplusplus", + "fastp" + ], + "fa_icon": "fas fa-hammer", + "description": "Specify which tool to use for complexity filtering" + }, + "shortread_complexityfilter_entropy": { + "type": "number", + "default": 0.3, + "fa_icon": "fas fa-random", + "description": "Specify the minimum sequence entropy level for complexity filtering", + "help_text": "Specify the minimum 'entropy' value for complexity filtering for BBDuk or PRINSEQ++.\n\nNote that this value will only be used for PRINSEQ++ if `--shortread_complexityfilter_prinseqplusplus_mode` is set to `entropy`.\n\nEntropy here corresponds to the amount of sequence variation exists within the read. Higher values correspond to more variety, and thus will likely reslut in more specific matching to a taxon's reference genome. The trade off here is fewer reads (or abundance information) available for having a confident identification.\n\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropy=`\n> - PRINSEQ++: `-lc_entropy`\n\n" + }, + "shortread_complexityfilter_bbduk_windowsize": { + "type": "integer", + "default": 50, + "fa_icon": "far fa-window-maximize", + "description": "Specify the window size for BBDuk complexity filtering", + "help_text": "Specify the window size to calculate the level entropy within for BBDuk.\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropywindow=`" + }, + "shortread_complexityfilter_bbduk_mask": { + "type": "boolean", + "fa_icon": "fas fa-mask", + "description": "Turn on masking rather than discarding of low complexity reads for BBduk", + "help_text": "Turn on masking of low-complexity reads (i.e., replacement with `N`) rather than removal.\n\n> Modifies tool parameter(s)\n> - BBDuk: `entropymask=`" + }, + "shortread_complexityfilter_fastp_threshold": { + "type": "integer", + "default": 30, + "fa_icon": "fas fa-sort-numeric-down", + "description": "Specify the minimum complexity filter threshold of fastp", + "help_text": "Specify the minimum sequence complexity value for fastp. This value corresponds to the percentage of bases that is different from it's adjacent bases.\n\n> Modifies tool parameter(s):\n> - removed from reads `--complexity_threshold`" + }, + "shortread_complexityfilter_prinseqplusplus_mode": { + "type": "string", + "default": "entropy", + "enum": [ + "entropy", + "dust" + ], + "fa_icon": "fas fa-check-square", + "description": "Specify the complexity filter mode for PRINSEQ++" + }, + "shortread_complexityfilter_prinseqplusplus_dustscore": { + "type": "number", + "default": 0.5, + "fa_icon": "fas fa-head-side-mask", + "description": "Specify the minimum dust score for PRINTSEQ++ complexity filtering", + "help_text": "Specify the minimum dust score below which low-complexity reads will be removed. A DUST score is based on how often different tri-nucleotides occur along a read.\n\n> Modifies tool parameter(s):\n> - PRINSEQ++: `--lc_dust`" + }, + "save_complexityfiltered_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save complexity filtered short-reads", + "help_text": "Specify whether to save the final complexity filtered reads in your results directory (`--outdir`)." + } + }, + "fa_icon": "fas fa-compress-alt" }, - "email": { - "type": "string", - "description": "Email address for completion summary.", - "fa_icon": "fas fa-envelope", - "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + "preprocessing_long_read_qc_options": { + "title": "Preprocessing long-read QC options", + "type": "object", + "description": "Options for adapter clipping, quality trimming, and length filtering", + "default": "", + "properties": { + "perform_longread_qc": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turns on long read quality control steps (adapter clipping, length filtering etc.)", + "help_text": "Turns on long read quality control steps (adapter clipping, length and/or quality filtering.)\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences.\n\nLength filtering, and quality filtering can speed up alignment by reducing the number of unspecific reads that need to be aligned." + }, + "longread_qc_skipadaptertrim": { + "type": "boolean", + "description": "Skip long-read trimming", + "fa_icon": "fas fa-forward", + "help_text": "Skip removal of adapters by Porechop. This can be useful in some cases to speed up run time - particularly when you are running data downloading from public databases such as the ENA/SRA that should already have adapters removed. We recommend that you check your FastQC results this is indeed the case." + }, + "longread_qc_skipqualityfilter": { + "type": "boolean", + "description": "Skip long-read length and quality filtering", + "fa_icon": "fas fa-forward", + "help_text": "Skip removal of quality filtering with Filtlong. This will skip length, percent reads, and target bases filtering (see other `--longread_qc_qualityfilter_*` parameters)." + }, + "longread_qc_qualityfilter_minlength": { + "type": "integer", + "default": 1000, + "description": "Specify the minimum length of reads to be retained", + "fa_icon": "fas fa-ruler-horizontal", + "help_text": "Specify the minimum of length of reads to be kept for downstream analysis.\n\n> Modifies tool parameter(s):\n> - Filtlong: `--min_length`" + }, + "longread_qc_qualityfilter_keeppercent": { + "type": "integer", + "default": 90, + "description": "Specify the percent of high-quality bases to be retained", + "fa_icon": "fas fa-percentage", + "help_text": "Throw out the remaining percentage of reads outside the value. This is measured by bp, not by read count. So this option throws out the worst e.g. 10% of read bases if the parameter is set to `90`. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" + }, + "longread_qc_qualityfilter_targetbases": { + "type": "integer", + "default": 500000000, + "description": "Specify the number of high-quality bases in the library to be retained", + "fa_icon": "fas fa-bullseye", + "help_text": "Removes the worst reads until only the specified value of bases remain, useful for very large read sets. If the input read set is less than the specified value, this setting will have no effect. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" + } + }, + "fa_icon": "fas fa-expand-alt" }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" + "preprocessing_host_removal_options": { + "title": "Preprocessing host removal options", + "type": "object", + "description": "Options for pre-profiling host read removal", + "default": "", + "properties": { + "perform_shortread_hostremoval": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on short-read host removal", + "help_text": "Turns on the ability to remove short-reads from the that derived from a known organism, using Bowtie2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." + }, + "perform_longread_hostremoval": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on long-read host removal", + "help_text": "Turns on the ability to remove long-reads from the that derived from a known organism, using minimap2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." + }, + "hostremoval_reference": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-file-alt", + "description": "Specify path to single reference FASTA of host(s) genome(s)", + "help_text": "Specify a path to the FASTA file (optionally gzipped) of the reference genome of the organism to be removed.\n\nIf you have two or more host organisms or contaminants you wish to remove, you can concatenate the FASTAs of the different taxa into a single one to provide to the pipeline." + }, + "shortread_hostremoval_index": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-address-book", + "description": "Specify path to the directory containing pre-made BowTie2 indexes of the host removal reference", + "help_text": "Specify the path to a _directory_ containing pre-made Bowtie2 reference index files (i.e. the directory containing `.bt1`, `.bt2` files etc.). These should sit in the same directory alongside the the reference file specified in `--hostremoval_reference`.\n\nSpecifying premade indices can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." + }, + "longread_hostremoval_index": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-address-book", + "description": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference", + "help_text": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference file given to `--hostremoval_reference`.\n\nSpecifying a premade index file can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." + }, + "save_hostremoval_index": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save mapping index of input reference when not already supplied by user", + "help_text": "Save the output files of the in-built indexing of the host genome.\n\nThis is recommend to be turned on if you plan to use the same reference genome multiple times, as supplying the directory or file to `--shortread_hostremoval_index` or `--longread_hostremoval_index` respectively can speed up runtime of future runs. Once generated, we recommend you place this file _outside_ of your run results directory in a central 'cache' directory you and others using your machine can access and supply to the pipeline." + }, + "save_hostremoval_mapped": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save mapped reads in BAM format from host removal", + "help_text": "Save the reads mapped to the reference genome in BAM format as output by the respective hostremoval alignment tool.\n\nThis can be useful if you wish to perform other analyses on the host organism (such as host-microbe interaction), however, you should consider whether the default mapping parameters of Bowtie2 (short-read) or minimap2 (long-read) are optimised to your context. " + }, + "save_hostremoval_unmapped": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save unmapped reads in FASTQ format from host removal", + "help_text": "Save the unreads mapped to the reference genome in FASTQ format (as exported from `samtools view`).\n\nThis can be useful if you wish to perform other analyses on the off-target reads from the host mapping, such as manual profiling or _de novo_ assembly." + } + }, + "fa_icon": "fas fa-user-times" + }, + "preprocessing_run_merging_options": { + "title": "Preprocessing run merging options", + "type": "object", + "description": "Options for per-sample run-merging", + "default": "", + "properties": { + "perform_runmerging": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on run merging", + "help_text": "Turns on the concatenation of sequencing runs or libraries with the same sample name.\n\nThis can be useful to ensure you get a single profile per sample, rather than one profile per run or library. Note that in some cases comparing profiles of independent _libraries_ may be useful, so this parameter may not always be suitable. " + }, + "save_runmerged_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Save run-concatenated input FASTQ files for each sample", + "help_text": "Save the run- and library-concatenated reads of a given sample in FASTQ format." + } + }, + "fa_icon": "fas fa-clipboard-check" + }, + "profiling_options": { + "title": "Profiling options", + "type": "object", + "description": "", + "default": "", + "properties": { + "run_centrifuge": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Centrifuge. Requires database to be present CSV file passed to --databases" + }, + "centrifuge_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Centrifuge-aligned reads", + "help_text": "Save mapped (SAM, FASTQ) and unmapped (FASTQ) reads from alignment step of centrifuge in your output results directory.\n\n> Modifies tool parameter(s):\n> - centrifuge: `--un-gz`, `--al-gz`, `--un-conc-gz`, `--al-conc-gz`, `--out-fmt`" + }, + "run_diamond": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with DIAMOND. Requires database to be present CSV file passed to --databases" + }, + "diamond_output_format": { + "type": "string", + "default": "tsv", + "enum": [ + "blast", + "xml", + "txt", + "daa", + "sam", + "tsv", + "paf" + ], + "fa_icon": "fas fa-file", + "description": "Specify output format from DIAMOND profiling.", + "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" + }, + "diamond_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of DIAMOND-aligned reads. Will override --diamond_output_format and no taxon tables will be generated", + "help_text": "Save aligned reads in SAM format from alignment step of DIAMOND in your output results directory.\n\nNote this explicitly overrides `--diamond_output_format` to produce the SAM file, and no taxon table will be generated.\n\n> Modifies tool parameter(s):\n> - DIAMOND: `--outfmt`" + }, + "run_kaiju": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Kaiju. Requires database to be present CSV file passed to --databases" + }, + "kaiju_taxon_rank": { + "type": "string", + "default": "species", + "enum": [ + "phylum", + "class", + "order", + "family", + "genus", + "species" + ], + "fa_icon": "fas fa-tag", + "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", + "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" + }, + "run_kraken2": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with Kraken2. Requires database to be present CSV file passed to --databases" + }, + "kraken2_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Kraken2-aligned reads", + "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - kraken2: `--classified-out` and `--unclassified-out`" + }, + "kraken2_save_readclassification": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of Kraken2 per-read taxonomic assignment file", + "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - kraken2: `--output`" + }, + "kraken2_save_minimizers": { + "type": "boolean", + "description": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.", + "fa_icon": "fas fa-save", + "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." + }, + "run_bracken": { + "type": "boolean", + "description": "Post-process kraken2 reports with Bracken.", + "fa_icon": "fas fa-toggle-on" + }, + "run_malt": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with MALT. Requires database to be present CSV file passed to --databases" + }, + "malt_mode": { + "type": "string", + "default": "BlastN", + "fa_icon": "fas fa-check-square", + "description": "Specify which MALT alignment mode to use", + "help_text": "Specify which version of MALT alignment to use.\n\nBlastN is generally recommended (nucleotide-nucleotide alignment), but particularly for very short reads (such as aDNA), whereas BlastX mode is similar to DIAMOND and will translate the nucleotide to amino acid sequences. Note each type of alignment mode requires different parameters during database construction. Refer to the MALT manual for more information.\n\n> Modifies tool parameter(s):\n> - malt-run: `-mode` " + }, + "malt_save_reads": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on saving of MALT-aligned reads", + "help_text": "Turns on saving of MALT aligned reads in SAM format.\n\nNote that the SAM format produce by MALT is not completely valid, and may not work with downstream tools.\n\n> Modifies tool parameter(s):\n> - malt-run: `--alignments`, `-za`" + }, + "malt_generate_megansummary": { + "type": "boolean", + "fa_icon": "fas fa-save", + "description": "Turn on generation of MEGAN summary file from MALT results", + "help_text": "Turns on saving of MALT output in an additional MEGAN summary file (`.megan`) that can be loaded into the MEGAN metagenomic exploration tool.\n\nNote: this file is generated not directly from MALT but rather then MEGAN utility script `rma2info`.\n\n> Modifies tool parameter(s):\n> - rma2info: `-es`" + }, + "run_metaphlan3": { + "type": "boolean", + "description": "Turn on profiling with MetaPhlAn3. Requires database to be present CSV file passed to --databases", + "fa_icon": "fas fa-toggle-on" + }, + "run_motus": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" + }, + "save_mgc_counts": { + "type": "boolean", + "description": "Save the mgc reads count for mOTUs." + }, + "save_read_counts": { + "type": "boolean", + "description": "Print mOTUs result as counts instead of relative abundances." + }, + "save_ncbi_id": { + "type": "boolean", + "description": "Print NCBI taxonomic id for mOTUs." + } + }, + "fa_icon": "fas fa-align-center" + }, + "postprocessing_and_visualisation_options": { + "title": "Postprocessing and visualisation options", + "type": "object", + "description": "", + "default": "", + "properties": { + "run_profile_standardisation": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on standardisation of taxon tables across profilers", + "help_text": "Turns on standardisation of output OTU tables across all tools; each into a TSV format following the following scheme:\n\n|TAXON | SAMPLE_A | SAMPLE_B |\n|-------------|----------------|-----------------|\n| taxon_a | 32 | 123 |\n| taxon_b | 1 | 5 |\n\nThis currently only is generated for mOTUs." + }, + "generate_biom_output": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on generation of BIOM output (currently only applies to mOTUs)", + "help_text": "Turn on the saving of the taxonomic output in BIOM format (`.biom`) in the results directory of your pipeline run, instead of the default TSV format.\n\nNote this file is from the output of the `motus merge` command.\n\n> Modifies tool parameter(s):\n> - `-B -o`" + }, + "run_krona": { + "type": "boolean", + "fa_icon": "fas fa-toggle-on", + "description": "Turn on generation of Krona plots for supported profilers", + "help_text": "Turn on the generation of Krona interactive pie-chart HTMLs for a selection of profilers.\n\nThe tools currently supported are:\n\n- centrifuge\n- kraken2\n- kaiju\n- MALT" + }, + "krona_taxonomy_directory": { + "type": "string", + "default": "None", + "fa_icon": "fas fa-folder-open", + "description": "Specify path to krona taxonomy directories (required for MALT krona plots)", + "help_text": "Specify a path to a Krona taxonomy database directory (i.e. a directory containing a krona generated `.tab` file).\n\nThis is only required for generating Krona plots of MALT output.\n\nNote this taxonomy database must be downloaded and generated with the `updateTaxonomy.sh` script from the krona-tools package." + } + }, + "fa_icon": "fas fa-chart-line" + }, + "institutional_config_options": { + "title": "Institutional config options", + "type": "object", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "properties": { + "custom_config_version": { + "type": "string", + "description": "Git commit id for Institutional configs.", + "default": "master", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "custom_config_base": { + "type": "string", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", + "hidden": true, + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" + }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], + "hidden": true + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, + "preprocessing_qc_tool": { + "type": "string", + "default": "fastqc", + "enum": [ + "fastqc", + "falco" + ], + "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", + "description": "Specify the tool used for quality control of raw sequencing reads" + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "hook_url": { + "type": "string", + "description": "Incoming hook URL for messaging service", + "fa_icon": "fas fa-people-group", + "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", + "hidden": true + }, + "multiqc_config": { + "type": "string", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_logo": { + "type": "string", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", + "hidden": true + }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, + "tracedir": { + "type": "string", + "description": "Directory to keep pipeline Nextflow logs and reports.", + "default": "${params.outdir}/pipeline_info", + "fa_icon": "fas fa-cogs", + "hidden": true + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "show_hidden_params": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "enable_conda": { + "type": "boolean", + "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", + "hidden": true, + "fa_icon": "fas fa-bacon" + } + } + }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Reference genome related files and options required for the workflow.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", + "hidden": true + }, + "igenomes_base": { + "type": "string", + "format": "directory-path", + "description": "Directory / URL base for iGenomes references.", + "default": "s3://ngi-igenomes/igenomes", + "fa_icon": "fas fa-cloud-download-alt", + "hidden": true + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } } - } }, - "preprocessing_general_qc_options": { - "title": "Preprocessing general QC options", - "type": "object", - "description": "Common options across both long and short read preprocessing QC steps", - "default": "", - "properties": { - "save_preprocessed_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save reads from adapter clipping/pair-merging, length filtering for both short and long reads", - "help_text": "This saves the FASTQ output from the following tools:\n\n- fastp\n- AdapterRemoval\n- Porechop\n- Filtlong\n\nThese reads will be a mixture of: adapter clipped, quality trimmed, pair-merged, and length filtered, depending on the parameters you set." + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/preprocessing_general_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_short_read_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_long_read_qc_options" + }, + { + "$ref": "#/definitions/preprocessing_host_removal_options" + }, + { + "$ref": "#/definitions/preprocessing_run_merging_options" + }, + { + "$ref": "#/definitions/profiling_options" + }, + { + "$ref": "#/definitions/postprocessing_and_visualisation_options" + }, + { + "$ref": "#/definitions/institutional_config_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/generic_options" + }, + { + "$ref": "#/definitions/reference_genome_options" } - }, - "fa_icon": "fas fa-users-cog" - }, - "preprocessing_short_read_qc_options": { - "title": "Preprocessing short-read QC options", - "type": "object", - "description": "Options for adapter clipping, quality trimming, pair-merging, and complexity filtering", - "default": "", - "properties": { - "perform_shortread_qc": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)", - "help_text": "Turns on short read quality control steps (adapter clipping, complexity filtering etc.)\n\nThis subworkflow can perform:\n\n- Adapter removal\n- Read quality trimming\n- Read pair merging\n- Length filtering\n- Complexity filtering\n\nEither with fastp or AdapterRemoval.\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences. Note that some, but not all, tools support paired-end alignment (utilising information about the insert covered by the pairs). However read pair merging in some cases can be recommend to increase read length (such as in aDNA). Length filtering, and/or complexity can speed up alignment by reducing the number of short unspecific reads that need to be aligned." - }, - "shortread_qc_tool": { - "type": "string", - "default": "fastp", - "enum": ["fastp", "adapterremoval"], - "fa_icon": "fas fa-tools", - "description": "Specify which tool to use for short-read QC" - }, - "shortread_qc_skipadaptertrim": { - "type": "boolean", - "fa_icon": "fas fa-forward", - "description": "Skip adapter trimming", - "help_text": "Skip the removal of sequencing adapters. \n\nThis often can be useful to speed up run-time of the pipeline when analysing data downloaded from public databases such as the ENA or SRA, as adapters should already be removed (however we recommend to check FastQC results to ensure this is the case)." - }, - "shortread_qc_adapter1": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-grip-lines", - "description": "Specify adapter 1 nucleotide sequence", - "help_text": "Specify a custom forward or R1 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCA`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG`" - }, - "shortread_qc_adapter2": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-grip-lines", - "description": "Specify adapter 2 nucleotide sequence", - "help_text": "Specify a custom reverse or R2 adapter sequence to be removed from reads. \n\nIf not set, the selected short-read QC tool's defaults will be used.\n\n> Modifies tool parameter(s):\n> - fastp: `--adapter_sequence`. fastp default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT`\n> - AdapterRemoval: `--adapter1`. AdapteRemoval2 default: `AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT`" - }, - "shortread_qc_adapterlist": { - "type": "string", - "default": "None", - "description": "Specify a list of all possible adapters to trim. Overrides --shortread_qc_adapter1/2. Formats: .txt (AdapterRemoval) or .fasta. (fastp).", - "help_text": "Allows to supply a file with a list of adapter (combinations) to remove from all files. \n\nOverrides the --shortread_qc_adapter1/--shortread_qc_adapter2 parameters . \n\nFor AdapterRemoval this consists of a two column table with a `.txt` extension: first column represents forward strand, second column for reverse strand. You must supply all possible combinations, one per line, and this list is applied to all files. See AdapterRemoval documentation for more information.\n\nFor fastp this consists of a standard FASTA format with a `.fasta`/`.fa`/`.fna`/`.fas` extension. The adapter sequence in this file should be at least 6bp long, otherwise it will be skipped. fastp trims the adapters present in the FASTA file one by one.\n\n> Modifies AdapterRemoval parameter: --adapter-list\n> Modifies fastp parameter: --adapter_fasta" - }, - "shortread_qc_mergepairs": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on merging of read pairs for paired-end data", - "default": true, - "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" - }, - "shortread_qc_excludeunmerged": { - "type": "boolean", - "fa_icon": "far fa-times-circle", - "description": "Discard unmerged reads from paired-end merging", - "help_text": "Turns off the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nThis can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - removed from reads `--include_unmerged`\n" - }, - "shortread_qc_minlength": { - "type": "integer", - "default": 15, - "fa_icon": "fas fa-ruler-horizontal", - "description": "Specify the minimum length of reads to be retained", - "help_text": "Specifying a mimum read length filtering can speed up profiling by reducing the number of short unspecific reads that need to be match/aligned to the database.\n\n> Modifies tool parameter(s):\n> - removed from reads `--length_required`\n> - AdapterRemoval: `--minlength`" - }, - "perform_shortread_complexityfilter": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on nucleotide sequence complexity filtering", - "help_text": "Turns on sequencing complexity filtering. Complexity filtering can be useful to increase run-time by removing unspecific read sequences that do not provide any informative taxon ID." - }, - "shortread_complexityfilter_tool": { - "type": "string", - "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], - "fa_icon": "fas fa-hammer", - "description": "Specify which tool to use for complexity filtering" - }, - "shortread_complexityfilter_entropy": { - "type": "number", - "default": 0.3, - "fa_icon": "fas fa-random", - "description": "Specify the minimum sequence entropy level for complexity filtering", - "help_text": "Specify the minimum 'entropy' value for complexity filtering for BBDuk or PRINSEQ++.\n\nNote that this value will only be used for PRINSEQ++ if `--shortread_complexityfilter_prinseqplusplus_mode` is set to `entropy`.\n\nEntropy here corresponds to the amount of sequence variation exists within the read. Higher values correspond to more variety, and thus will likely reslut in more specific matching to a taxon's reference genome. The trade off here is fewer reads (or abundance information) available for having a confident identification.\n\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropy=`\n> - PRINSEQ++: `-lc_entropy`\n\n" - }, - "shortread_complexityfilter_bbduk_windowsize": { - "type": "integer", - "default": 50, - "fa_icon": "far fa-window-maximize", - "description": "Specify the window size for BBDuk complexity filtering", - "help_text": "Specify the window size to calculate the level entropy within for BBDuk.\n\n> Modifies tool parameter(s):\n> - BBDuk: `entropywindow=`" - }, - "shortread_complexityfilter_bbduk_mask": { - "type": "boolean", - "fa_icon": "fas fa-mask", - "description": "Turn on masking rather than discarding of low complexity reads for BBduk", - "help_text": "Turn on masking of low-complexity reads (i.e., replacement with `N`) rather than removal.\n\n> Modifies tool parameter(s)\n> - BBDuk: `entropymask=`" - }, - "shortread_complexityfilter_fastp_threshold": { - "type": "integer", - "default": 30, - "fa_icon": "fas fa-sort-numeric-down", - "description": "Specify the minimum complexity filter threshold of fastp", - "help_text": "Specify the minimum sequence complexity value for fastp. This value corresponds to the percentage of bases that is different from it's adjacent bases.\n\n> Modifies tool parameter(s):\n> - removed from reads `--complexity_threshold`" - }, - "shortread_complexityfilter_prinseqplusplus_mode": { - "type": "string", - "default": "entropy", - "enum": ["entropy", "dust"], - "fa_icon": "fas fa-check-square", - "description": "Specify the complexity filter mode for PRINSEQ++" - }, - "shortread_complexityfilter_prinseqplusplus_dustscore": { - "type": "number", - "default": 0.5, - "fa_icon": "fas fa-head-side-mask", - "description": "Specify the minimum dust score for PRINTSEQ++ complexity filtering", - "help_text": "Specify the minimum dust score below which low-complexity reads will be removed. A DUST score is based on how often different tri-nucleotides occur along a read.\n\n> Modifies tool parameter(s):\n> - PRINSEQ++: `--lc_dust`" - }, - "save_complexityfiltered_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save complexity filtered short-reads", - "help_text": "Specify whether to save the final complexity filtered reads in your results directory (`--outdir`)." - } - }, - "fa_icon": "fas fa-compress-alt" - }, - "preprocessing_long_read_qc_options": { - "title": "Preprocessing long-read QC options", - "type": "object", - "description": "Options for adapter clipping, quality trimming, and length filtering", - "default": "", - "properties": { - "perform_longread_qc": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turns on long read quality control steps (adapter clipping, length filtering etc.)", - "help_text": "Turns on long read quality control steps (adapter clipping, length and/or quality filtering.)\n\nRemoving adapters (if present) is recommend to reduce false-postive hits that may occur from 'dirty' or 'contaminated' reference genomes in a profiling database that contain accidentially incorporated adapter sequences.\n\nLength filtering, and quality filtering can speed up alignment by reducing the number of unspecific reads that need to be aligned." - }, - "longread_qc_skipadaptertrim": { - "type": "boolean", - "description": "Skip long-read trimming", - "fa_icon": "fas fa-forward", - "help_text": "Skip removal of adapters by Porechop. This can be useful in some cases to speed up run time - particularly when you are running data downloading from public databases such as the ENA/SRA that should already have adapters removed. We recommend that you check your FastQC results this is indeed the case." - }, - "longread_qc_skipqualityfilter": { - "type": "boolean", - "description": "Skip long-read length and quality filtering", - "fa_icon": "fas fa-forward", - "help_text": "Skip removal of quality filtering with Filtlong. This will skip length, percent reads, and target bases filtering (see other `--longread_qc_qualityfilter_*` parameters)." - }, - "longread_qc_qualityfilter_minlength": { - "type": "integer", - "default": 1000, - "description": "Specify the minimum length of reads to be retained", - "fa_icon": "fas fa-ruler-horizontal", - "help_text": "Specify the minimum of length of reads to be kept for downstream analysis.\n\n> Modifies tool parameter(s):\n> - Filtlong: `--min_length`" - }, - "longread_qc_qualityfilter_keeppercent": { - "type": "integer", - "default": 90, - "description": "Specify the percent of high-quality bases to be retained", - "fa_icon": "fas fa-percentage", - "help_text": "Throw out the remaining percentage of reads outside the value. This is measured by bp, not by read count. So this option throws out the worst e.g. 10% of read bases if the parameter is set to `90`. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" - }, - "longread_qc_qualityfilter_targetbases": { - "type": "integer", - "default": 500000000, - "description": "Specify the number of high-quality bases in the library to be retained", - "fa_icon": "fas fa-bullseye", - "help_text": "Removes the worst reads until only the specified value of bases remain, useful for very large read sets. If the input read set is less than the specified value, this setting will have no effect. _Modified from [Filtlong documentation](https://github.com/rrwick/Filtlong)_\n\n> Modifies tool parameter(s):\n> - Filtlong: `--keep_percent`" - } - }, - "fa_icon": "fas fa-expand-alt" - }, - "preprocessing_host_removal_options": { - "title": "Preprocessing host removal options", - "type": "object", - "description": "Options for pre-profiling host read removal", - "default": "", - "properties": { - "perform_shortread_hostremoval": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on short-read host removal", - "help_text": "Turns on the ability to remove short-reads from the that derived from a known organism, using Bowtie2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." - }, - "perform_longread_hostremoval": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on long-read host removal", - "help_text": "Turns on the ability to remove long-reads from the that derived from a known organism, using minimap2 and samtools\n\nThis subworkflow is useful to remove reads that may come from a host, or a known contamination like the human reference genome. Human DNA contamination of (microbial) reference genomes is well known, so removal of these prior profiling both reduces the risks of false positives, and in _some cases_ a faster runtime (as less reads need to be profiled).\n\nAlternatively, you can include the reference genome within your profiling databases and can turn off this subworkflow, with the trade off of a larger taxonomic profiling database." - }, - "hostremoval_reference": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-file-alt", - "description": "Specify path to single reference FASTA of host(s) genome(s)", - "help_text": "Specify a path to the FASTA file (optionally gzipped) of the reference genome of the organism to be removed.\n\nIf you have two or more host organisms or contaminants you wish to remove, you can concatenate the FASTAs of the different taxa into a single one to provide to the pipeline." - }, - "shortread_hostremoval_index": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-address-book", - "description": "Specify path to the directory containing pre-made BowTie2 indexes of the host removal reference", - "help_text": "Specify the path to a _directory_ containing pre-made Bowtie2 reference index files (i.e. the directory containing `.bt1`, `.bt2` files etc.). These should sit in the same directory alongside the the reference file specified in `--hostremoval_reference`.\n\nSpecifying premade indices can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." - }, - "longread_hostremoval_index": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-address-book", - "description": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference", - "help_text": "Specify path to a pre-made Minimap2 index file (.mmi) of the host removal reference file given to `--hostremoval_reference`.\n\nSpecifying a premade index file can speed up runtime of the host-removal step, however if not supplied the pipeline will generate the indices for you." - }, - "save_hostremoval_index": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save mapping index of input reference when not already supplied by user", - "help_text": "Save the output files of the in-built indexing of the host genome.\n\nThis is recommend to be turned on if you plan to use the same reference genome multiple times, as supplying the directory or file to `--shortread_hostremoval_index` or `--longread_hostremoval_index` respectively can speed up runtime of future runs. Once generated, we recommend you place this file _outside_ of your run results directory in a central 'cache' directory you and others using your machine can access and supply to the pipeline." - }, - "save_hostremoval_mapped": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save mapped reads in BAM format from host removal", - "help_text": "Save the reads mapped to the reference genome in BAM format as output by the respective hostremoval alignment tool.\n\nThis can be useful if you wish to perform other analyses on the host organism (such as host-microbe interaction), however, you should consider whether the default mapping parameters of Bowtie2 (short-read) or minimap2 (long-read) are optimised to your context. " - }, - "save_hostremoval_unmapped": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save unmapped reads in FASTQ format from host removal", - "help_text": "Save the unreads mapped to the reference genome in FASTQ format (as exported from `samtools view`).\n\nThis can be useful if you wish to perform other analyses on the off-target reads from the host mapping, such as manual profiling or _de novo_ assembly." - } - }, - "fa_icon": "fas fa-user-times" - }, - "preprocessing_run_merging_options": { - "title": "Preprocessing run merging options", - "type": "object", - "description": "Options for per-sample run-merging", - "default": "", - "properties": { - "perform_runmerging": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on run merging", - "help_text": "Turns on the concatenation of sequencing runs or libraries with the same sample name.\n\nThis can be useful to ensure you get a single profile per sample, rather than one profile per run or library. Note that in some cases comparing profiles of independent _libraries_ may be useful, so this parameter may not always be suitable. " - }, - "save_runmerged_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Save run-concatenated input FASTQ files for each sample", - "help_text": "Save the run- and library-concatenated reads of a given sample in FASTQ format." - } - }, - "fa_icon": "fas fa-clipboard-check" - }, - "profiling_options": { - "title": "Profiling options", - "type": "object", - "description": "", - "default": "", - "properties": { - "run_centrifuge": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Centrifuge. Requires database to be present CSV file passed to --databases" - }, - "centrifuge_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Centrifuge-aligned reads", - "help_text": "Save mapped (SAM, FASTQ) and unmapped (FASTQ) reads from alignment step of centrifuge in your output results directory.\n\n> Modifies tool parameter(s):\n> - centrifuge: `--un-gz`, `--al-gz`, `--un-conc-gz`, `--al-conc-gz`, `--out-fmt`" - }, - "run_diamond": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with DIAMOND. Requires database to be present CSV file passed to --databases" - }, - "diamond_output_format": { - "type": "string", - "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], - "fa_icon": "fas fa-file", - "description": "Specify output format from DIAMOND profiling.", - "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" - }, - "diamond_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of DIAMOND-aligned reads. Will override --diamond_output_format and no taxon tables will be generated", - "help_text": "Save aligned reads in SAM format from alignment step of DIAMOND in your output results directory.\n\nNote this explicitly overrides `--diamond_output_format` to produce the SAM file, and no taxon table will be generated.\n\n> Modifies tool parameter(s):\n> - DIAMOND: `--outfmt`" - }, - "run_kaiju": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Kaiju. Requires database to be present CSV file passed to --databases" - }, - "kaiju_taxon_rank": { - "type": "string", - "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], - "fa_icon": "fas fa-tag", - "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", - "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" - }, - "run_kraken2": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with Kraken2. Requires database to be present CSV file passed to --databases" - }, - "kraken2_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Kraken2-aligned reads", - "help_text": "Save reads that do and do not have a taxonomic classification in your output results directory in FASTQ format.\n\n> Modifies tool parameter(s):\n> - kraken2: `--classified-out` and `--unclassified-out`" - }, - "kraken2_save_readclassification": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of Kraken2 per-read taxonomic assignment file", - "help_text": "Save a text file that contains a list of each read that had a taxonomic assignment, with information on specific taxonomic taxonomic assignment that that read recieved.\n\n> Modifies tool parameter(s):\n> - kraken2: `--output`" - }, - "kraken2_save_minimizers": { - "type": "boolean", - "description": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.", - "fa_icon": "fas fa-save", - "help_text": "Turn on saving minimizer information in the kraken2 report thus increasing to an eight column layout.\n\nAdds `--report-minimizer-data` to the kraken2 command." - }, - "run_bracken": { - "type": "boolean", - "description": "Post-process kraken2 reports with Bracken.", - "fa_icon": "fas fa-toggle-on" - }, - "run_malt": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with MALT. Requires database to be present CSV file passed to --databases" - }, - "malt_mode": { - "type": "string", - "default": "BlastN", - "fa_icon": "fas fa-check-square", - "description": "Specify which MALT alignment mode to use", - "help_text": "Specify which version of MALT alignment to use.\n\nBlastN is generally recommended (nucleotide-nucleotide alignment), but particularly for very short reads (such as aDNA), whereas BlastX mode is similar to DIAMOND and will translate the nucleotide to amino acid sequences. Note each type of alignment mode requires different parameters during database construction. Refer to the MALT manual for more information.\n\n> Modifies tool parameter(s):\n> - malt-run: `-mode` " - }, - "malt_save_reads": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on saving of MALT-aligned reads", - "help_text": "Turns on saving of MALT aligned reads in SAM format.\n\nNote that the SAM format produce by MALT is not completely valid, and may not work with downstream tools.\n\n> Modifies tool parameter(s):\n> - malt-run: `--alignments`, `-za`" - }, - "malt_generate_megansummary": { - "type": "boolean", - "fa_icon": "fas fa-save", - "description": "Turn on generation of MEGAN summary file from MALT results", - "help_text": "Turns on saving of MALT output in an additional MEGAN summary file (`.megan`) that can be loaded into the MEGAN metagenomic exploration tool.\n\nNote: this file is generated not directly from MALT but rather then MEGAN utility script `rma2info`.\n\n> Modifies tool parameter(s):\n> - rma2info: `-es`" - }, - "run_metaphlan3": { - "type": "boolean", - "description": "Turn on profiling with MetaPhlAn3. Requires database to be present CSV file passed to --databases", - "fa_icon": "fas fa-toggle-on" - }, - "run_motus": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" - } - }, - "fa_icon": "fas fa-align-center" - }, - "postprocessing_and_visualisation_options": { - "title": "Postprocessing and visualisation options", - "type": "object", - "description": "", - "default": "", - "properties": { - "run_profile_standardisation": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on standardisation of taxon tables across profilers", - "help_text": "Turns on standardisation of output OTU tables across all tools; each into a TSV format following the following scheme:\n\n|TAXON | SAMPLE_A | SAMPLE_B |\n|-------------|----------------|-----------------|\n| taxon_a | 32 | 123 |\n| taxon_b | 1 | 5 |\n\nThis currently only is generated for mOTUs." - }, - "generate_biom_output": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on generation of BIOM output (currently only applies to mOTUs)", - "help_text": "Turn on the saving of the taxonomic output in BIOM format (`.biom`) in the results directory of your pipeline run, instead of the default TSV format.\n\nNote this file is from the output of the `motus merge` command.\n\n> Modifies tool parameter(s):\n> - `-B -o`" - }, - "run_krona": { - "type": "boolean", - "fa_icon": "fas fa-toggle-on", - "description": "Turn on generation of Krona plots for supported profilers", - "help_text": "Turn on the generation of Krona interactive pie-chart HTMLs for a selection of profilers.\n\nThe tools currently supported are:\n\n- centrifuge\n- kraken2\n- kaiju\n- MALT" - }, - "krona_taxonomy_directory": { - "type": "string", - "default": "None", - "fa_icon": "fas fa-folder-open", - "description": "Specify path to krona taxonomy directories (required for MALT krona plots)", - "help_text": "Specify a path to a Krona taxonomy database directory (i.e. a directory containing a krona generated `.tab` file).\n\nThis is only required for generating Krona plots of MALT output.\n\nNote this taxonomy database must be downloaded and generated with the `updateTaxonomy.sh` script from the krona-tools package." - } - }, - "fa_icon": "fas fa-chart-line" - }, - "institutional_config_options": { - "title": "Institutional config options", - "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", - "properties": { - "custom_config_version": { - "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "custom_config_base": { - "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", - "fa_icon": "fas fa-users-cog" - }, - "config_profile_name": { - "type": "string", - "description": "Institutional config name.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_description": { - "type": "string", - "description": "Institutional config description.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - } - } - }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } - }, - "generic_options": { - "title": "Generic options", - "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", - "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, - "publish_dir_mode": { - "type": "string", - "default": "copy", - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], - "hidden": true - }, - "email_on_fail": { - "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", - "hidden": true - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", - "hidden": true - }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true - }, - "preprocessing_qc_tool": { - "type": "string", - "default": "fastqc", - "enum": ["fastqc", "falco"], - "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", - "description": "Specify the tool used for quality control of raw sequencing reads" - }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", - "hidden": true - }, - "hook_url": { - "type": "string", - "description": "Incoming hook URL for messaging service", - "fa_icon": "fas fa-people-group", - "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", - "hidden": true - }, - "multiqc_config": { - "type": "string", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "multiqc_logo": { - "type": "string", - "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", - "fa_icon": "fas fa-image", - "hidden": true - }, - "multiqc_methods_description": { - "type": "string", - "description": "Custom MultiQC yaml file containing HTML including a methods description.", - "fa_icon": "fas fa-cog" - }, - "tracedir": { - "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "show_hidden_params": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "enable_conda": { - "type": "boolean", - "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", - "hidden": true, - "fa_icon": "fas fa-bacon" - } - } - }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details.", - "hidden": true - }, - "igenomes_base": { - "type": "string", - "format": "directory-path", - "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes", - "fa_icon": "fas fa-cloud-download-alt", - "hidden": true - }, - "igenomes_ignore": { - "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." - } - } - } - }, - "allOf": [ - { - "$ref": "#/definitions/input_output_options" - }, - { - "$ref": "#/definitions/preprocessing_general_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_short_read_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_long_read_qc_options" - }, - { - "$ref": "#/definitions/preprocessing_host_removal_options" - }, - { - "$ref": "#/definitions/preprocessing_run_merging_options" - }, - { - "$ref": "#/definitions/profiling_options" - }, - { - "$ref": "#/definitions/postprocessing_and_visualisation_options" - }, - { - "$ref": "#/definitions/institutional_config_options" - }, - { - "$ref": "#/definitions/max_job_request_options" - }, - { - "$ref": "#/definitions/generic_options" - }, - { - "$ref": "#/definitions/reference_genome_options" - } - ], - "properties": { - "save_mgc_counts": { - "type": "boolean", - "description": "Save the mgc reads count for mOTUs." - }, - "save_ncbi_id": { - "type": "boolean", - "description": "Print NCBI taxonomic id for mOTUs." - }, - "save_read_counts": { - "type": "boolean", - "description": "Print mOTUs result as counts instead of relative abundances." - } - } -} + ] +} \ No newline at end of file From 933b820c198808b5d6a5336b0bae2b2a369eb982 Mon Sep 17 00:00:00 2001 From: sofstam Date: Tue, 29 Nov 2022 16:57:07 +0100 Subject: [PATCH 113/132] Apply review suggestions --- conf/modules.config | 6 +++--- conf/test_motus.config | 6 +++--- nextflow.config | 6 +++--- nextflow_schema.json | 29 +++++++++++------------------ 4 files changed, 20 insertions(+), 27 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 0f54301..63be9ff 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -448,9 +448,9 @@ process { withName: MOTUS_PROFILE { ext.args = { [ - params.save_ncbi_id ? "-p" : "", - params.save_read_counts ? "-c" : "", - params.save_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" + params.motus_ncbi_id ? "-p" : "", + params.motus_read_counts ? "-c" : "", + params.motus_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" ].join(',').replaceAll(','," ") } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } diff --git a/conf/test_motus.config b/conf/test_motus.config index 70681dd..8eb324b 100644 --- a/conf/test_motus.config +++ b/conf/test_motus.config @@ -39,8 +39,8 @@ params { run_centrifuge = false run_diamond = false run_motus = true - save_mgc_counts = true - save_ncbi_id = true - save_read_counts = true + motus_mgc_counts = true + motus_ncbi_id = true + motus_read_counts = true run_profile_standardisation = true } diff --git a/nextflow.config b/nextflow.config index 91875b2..c9bb0f4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -140,9 +140,9 @@ params { // mOTUs run_motus = false - save_mgc_counts = false - save_ncbi_id = false - save_read_counts = false + motus_mgc_counts = false + motus_ncbi_id = false + motus_read_counts = false // krona run_krona = false diff --git a/nextflow_schema.json b/nextflow_schema.json index cec0ab7..0b20762 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -63,7 +63,10 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": ["fastqc", "falco"], + "enum": [ + "fastqc", + "falco" + ], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "Specify the tool used for quality control of raw sequencing reads", "fa_icon": "fas fa-tools" @@ -470,17 +473,17 @@ "fa_icon": "fas fa-toggle-on", "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" }, - "save_mgc_counts": { + "motus_mgc_counts": { "type": "boolean", - "description": "Save the mgc reads count for mOTUs." + "description": "Save the mgc reads count." }, - "save_read_counts": { + "motus_ncbi_id": { "type": "boolean", - "description": "Print mOTUs result as counts instead of relative abundances." + "description": "Print NCBI id." }, - "save_ncbi_id": { + "motus_read_counts": { "type": "boolean", - "description": "Print NCBI taxonomic id for mOTUs." + "description": "Print result as counts instead of relative abundances." } }, "fa_icon": "fas fa-align-center" @@ -653,16 +656,6 @@ "fa_icon": "fas fa-file-upload", "hidden": true }, - "preprocessing_qc_tool": { - "type": "string", - "default": "fastqc", - "enum": [ - "fastqc", - "falco" - ], - "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", - "description": "Specify the tool used for quality control of raw sequencing reads" - }, "monochrome_logs": { "type": "boolean", "description": "Do not use coloured log outputs.", @@ -791,4 +784,4 @@ "$ref": "#/definitions/reference_genome_options" } ] -} \ No newline at end of file +} From 8067012c01c1e29803a5365529cd139d463543eb Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 1 Dec 2022 09:15:35 +0000 Subject: [PATCH 114/132] [automated] Fix linting with Prettier --- nextflow_schema.json | 55 +++++++------------------------------------- 1 file changed, 8 insertions(+), 47 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 0b20762..2fcdd80 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,11 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "databases", - "outdir" - ], + "required": ["input", "databases", "outdir"], "properties": { "input": { "type": "string", @@ -63,10 +59,7 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": [ - "fastqc", - "falco" - ], + "enum": ["fastqc", "falco"], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "Specify the tool used for quality control of raw sequencing reads", "fa_icon": "fas fa-tools" @@ -95,10 +88,7 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], + "enum": ["fastp", "adapterremoval"], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -157,11 +147,7 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], + "enum": ["bbduk", "prinseqplusplus", "fastp"], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -195,10 +181,7 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": [ - "entropy", - "dust" - ], + "enum": ["entropy", "dust"], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -372,15 +355,7 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -399,14 +374,7 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], + "enum": ["phylum", "class", "order", "family", "genus", "species"], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -624,14 +592,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { From bc98f5b80f24b77d3168412e81e7e7feaf043bc0 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 1 Dec 2022 10:37:14 +0100 Subject: [PATCH 115/132] Separate KrakenUniq test run --- .github/workflows/ci.yml | 33 +++++++++++++++++ conf/test_krakenuniq.config | 72 +++++++++++++++++++++++++++++++++++++ conf/test_motus.config | 4 +++ nextflow.config | 1 + 4 files changed, 110 insertions(+) create mode 100644 conf/test_krakenuniq.config diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index a2a1662..77440b7 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -101,3 +101,36 @@ jobs: with: command: nextflow run ${GITHUB_WORKSPACE} -profile test_motus,docker --outdir ./results --databases ./database_motus.csv attempt_limit: 3 + + krakenuniq: + name: Test mOTUs with workflow parameters + if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/taxprofiler') }} + runs-on: ubuntu-latest + strategy: + matrix: + NXF_VER: + - "21.10.3" + - "latest-everything" + + steps: + - name: Check out pipeline code + uses: actions/checkout@v2 + + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + with: + version: "${{ matrix.NXF_VER }}" + + - name: Show current locale + run: locale + + - name: Set UTF-8 enabled locale + run: | + sudo locale-gen en_US.UTF-8 + sudo update-locale LANG=en_US.UTF-8 + + - name: Run pipeline with test data + uses: Wandalen/wretry.action@v1.0.11 + with: + command: nextflow run ${GITHUB_WORKSPACE} -profile test_krakenuniq,docker --outdir ./results + attempt_limit: 3 diff --git a/conf/test_krakenuniq.config b/conf/test_krakenuniq.config new file mode 100644 index 0000000..c6e2548 --- /dev/null +++ b/conf/test_krakenuniq.config @@ -0,0 +1,72 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/taxprofiler -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +// +// Separate test as KrakenUniq database can sometimes be too big for GHA +// + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test to check mOTUs function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/taxprofiler/samplesheet.csv' + databases = 'https://raw.githubusercontent.com/nf-core/test-datasets/taxprofiler/database_krakenuniq.csv' + perform_shortread_qc = true + perform_longread_qc = true + shortread_qc_mergepairs = true + perform_shortread_complexityfilter = true + perform_shortread_hostremoval = true + perform_longread_hostremoval = true + perform_runmerging = true + hostremoval_reference = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' + run_kaiju = false` + run_kraken2 = false` + run_bracken = false + run_malt = false + run_metaphlan3 = false + run_centrifuge = false + run_diamond = false + run_krakenuniq = true + run_motus = false + run_krona = true + krona_taxonomy_directory = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/sarscov2/metagenome/krona_taxonomy.tab' + malt_save_reads = true + kraken2_save_reads = true + centrifuge_save_reads = true + diamond_save_reads = true +} + +process { + withName: MALT_RUN { + maxForks = 1 + } + withName: MEGAN_RMA2INFO_TSV { + maxForks = 1 + } + withName: MEGAN_RMA2INFO_KRONA { + maxForks = 1 + } + withName: 'EIDO_VALIDATE' { + ext.args = '--st-index sample' + } + withName: 'EIDO_CONVERT' { + ext.args = '--st-index sample' + } +} diff --git a/conf/test_motus.config b/conf/test_motus.config index 2428a4c..a077dad 100644 --- a/conf/test_motus.config +++ b/conf/test_motus.config @@ -10,6 +10,10 @@ ---------------------------------------------------------------------------------------- */ +// +// Separate test as mOTUs database download can be flaky +// + params { config_profile_name = 'mOTUs Test profile' config_profile_description = 'Minimal test to check mOTUs function' diff --git a/nextflow.config b/nextflow.config index 9ad8def..721a21c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -242,6 +242,7 @@ profiles { test_nopreprocessing { includeConfig 'conf/test_nopreprocessing.config' } test_nothing { includeConfig 'conf/test_nothing.config' } test_motus { includeConfig 'conf/test_motus.config' } + test_krakenuniq { includeConfig 'conf/test_krakenuniq.config' } test_pep { includeConfig 'conf/test_pep.config' } } From e5bb627402179b42ef817845db8c99a0546c4a16 Mon Sep 17 00:00:00 2001 From: sofstam Date: Thu, 1 Dec 2022 11:12:31 +0100 Subject: [PATCH 116/132] Apply review suggestions --- conf/modules.config | 6 ++-- conf/test_motus.config | 6 ++-- nextflow.config | 6 ++-- nextflow_schema.json | 73 ++++++++++++++++++++++++++++++++---------- 4 files changed, 65 insertions(+), 26 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 63be9ff..1ff6f55 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -448,9 +448,9 @@ process { withName: MOTUS_PROFILE { ext.args = { [ - params.motus_ncbi_id ? "-p" : "", - params.motus_read_counts ? "-c" : "", - params.motus_mgc_counts ? "-M ${task.ext.prefix}.mgc" : "" + params.motus_use_ncbi_ids ? "-p" : "", + params.motus_output_type ? "-c" : "", + params.motus_save_mgcreadcounts ? "-M ${task.ext.prefix}.mgc" : "" ].join(',').replaceAll(','," ") } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } diff --git a/conf/test_motus.config b/conf/test_motus.config index 8eb324b..b5c2c51 100644 --- a/conf/test_motus.config +++ b/conf/test_motus.config @@ -39,8 +39,8 @@ params { run_centrifuge = false run_diamond = false run_motus = true - motus_mgc_counts = true - motus_ncbi_id = true - motus_read_counts = true + motus_save_mgcreadcounts = true + motus_use_ncbi_ids = true + motus_output_type = true run_profile_standardisation = true } diff --git a/nextflow.config b/nextflow.config index c9bb0f4..b1da76a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -140,9 +140,9 @@ params { // mOTUs run_motus = false - motus_mgc_counts = false - motus_ncbi_id = false - motus_read_counts = false + motus_output_type = false + motus_use_ncbi_ids = false + motus_save_mgcreadcounts = false // krona run_krona = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 2fcdd80..7c4998a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,11 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "databases", "outdir"], + "required": [ + "input", + "databases", + "outdir" + ], "properties": { "input": { "type": "string", @@ -59,7 +63,10 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": ["fastqc", "falco"], + "enum": [ + "fastqc", + "falco" + ], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "Specify the tool used for quality control of raw sequencing reads", "fa_icon": "fas fa-tools" @@ -88,7 +95,10 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": ["fastp", "adapterremoval"], + "enum": [ + "fastp", + "adapterremoval" + ], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -147,7 +157,11 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": ["bbduk", "prinseqplusplus", "fastp"], + "enum": [ + "bbduk", + "prinseqplusplus", + "fastp" + ], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -181,7 +195,10 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": ["entropy", "dust"], + "enum": [ + "entropy", + "dust" + ], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -355,7 +372,15 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], + "enum": [ + "blast", + "xml", + "txt", + "daa", + "sam", + "tsv", + "paf" + ], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -374,7 +399,14 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": ["phylum", "class", "order", "family", "genus", "species"], + "enum": [ + "phylum", + "class", + "order", + "family", + "genus", + "species" + ], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -441,17 +473,17 @@ "fa_icon": "fas fa-toggle-on", "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" }, - "motus_mgc_counts": { - "type": "boolean", - "description": "Save the mgc reads count." + "motus_use_ncbi_ids": { + "type": "string", + "default": "false" }, - "motus_ncbi_id": { - "type": "boolean", - "description": "Print NCBI id." + "motus_save_mgcreadcounts": { + "type": "string", + "default": "false" }, - "motus_read_counts": { - "type": "boolean", - "description": "Print result as counts instead of relative abundances." + "motus_output_type": { + "type": "string", + "default": "false" } }, "fa_icon": "fas fa-align-center" @@ -592,7 +624,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { From 1965ade961c93c5efaf8fc6fee93af9838a788a8 Mon Sep 17 00:00:00 2001 From: sofstam Date: Thu, 1 Dec 2022 11:32:51 +0100 Subject: [PATCH 117/132] Update schema --- nextflow_schema.json | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 7c4998a..2864a52 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -474,16 +474,16 @@ "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" }, "motus_use_ncbi_ids": { - "type": "string", - "default": "false" + "type": "boolean", + "description": "Turn on printing ncbi taxonomic ids." }, "motus_save_mgcreadcounts": { - "type": "string", - "default": "false" + "type": "boolean", + "description": "Turn on saving the mgc reads count to a separate file." }, "motus_output_type": { - "type": "string", - "default": "false" + "type": "boolean", + "description": "Turn on printing result as counts instead of relative abundances." } }, "fa_icon": "fas fa-align-center" From ef1d2da17917abd883d8937197fc92bb63ab372c Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Thu, 1 Dec 2022 12:53:34 +0100 Subject: [PATCH 118/132] Apply suggestions from code review Co-authored-by: Moritz E. Beber --- .github/workflows/ci.yml | 2 +- conf/test_krakenuniq.config | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 77440b7..d179c6e 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -103,7 +103,7 @@ jobs: attempt_limit: 3 krakenuniq: - name: Test mOTUs with workflow parameters + name: Test KrakenUniq with workflow parameters if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/taxprofiler') }} runs-on: ubuntu-latest strategy: diff --git a/conf/test_krakenuniq.config b/conf/test_krakenuniq.config index c6e2548..c504ec9 100644 --- a/conf/test_krakenuniq.config +++ b/conf/test_krakenuniq.config @@ -16,7 +16,7 @@ params { config_profile_name = 'Test profile' - config_profile_description = 'Minimal test to check mOTUs function' + config_profile_description = 'Minimal test to check KrakenUniq function' // Limit resources so that this can run on GitHub Actions max_cpus = 2 From 38a818fd174718229385a0ed301c42c76cadb9ec Mon Sep 17 00:00:00 2001 From: sofstam Date: Thu, 1 Dec 2022 13:42:21 +0100 Subject: [PATCH 119/132] Apply review suggestions --- conf/modules.config | 6 +++--- conf/test_motus.config | 6 +++--- nextflow.config | 8 ++++---- nextflow_schema.json | 28 +++++++++++++++------------- 4 files changed, 25 insertions(+), 23 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 1ff6f55..43e179d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -448,9 +448,9 @@ process { withName: MOTUS_PROFILE { ext.args = { [ - params.motus_use_ncbi_ids ? "-p" : "", - params.motus_output_type ? "-c" : "", - params.motus_save_mgcreadcounts ? "-M ${task.ext.prefix}.mgc" : "" + params.motus_remove_ncbi_ids ? "" : "-p", + params.motus_use_relative_abundance ? "" : "-c", + params.motus_save_mgc_read_counts ? "-M ${task.ext.prefix}.mgc" : "" ].join(',').replaceAll(','," ") } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } diff --git a/conf/test_motus.config b/conf/test_motus.config index b5c2c51..92c9a91 100644 --- a/conf/test_motus.config +++ b/conf/test_motus.config @@ -39,8 +39,8 @@ params { run_centrifuge = false run_diamond = false run_motus = true - motus_save_mgcreadcounts = true - motus_use_ncbi_ids = true - motus_output_type = true + motus_save_mgc_read_counts = true + motus_remove_ncbi_ids = true + motus_use_relative_abundance = true run_profile_standardisation = true } diff --git a/nextflow.config b/nextflow.config index b1da76a..84c5242 100644 --- a/nextflow.config +++ b/nextflow.config @@ -139,10 +139,10 @@ params { diamond_save_reads = false // this will override default diamond output format so no taxonomic profile is generated! added directly to module in profiling.nf // mOTUs - run_motus = false - motus_output_type = false - motus_use_ncbi_ids = false - motus_save_mgcreadcounts = false + run_motus = false + motus_use_relative_abundance = false + motus_remove_ncbi_ids = false + motus_save_mgc_read_counts = false // krona run_krona = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 2864a52..e6db686 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -472,18 +472,6 @@ "type": "boolean", "fa_icon": "fas fa-toggle-on", "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" - }, - "motus_use_ncbi_ids": { - "type": "boolean", - "description": "Turn on printing ncbi taxonomic ids." - }, - "motus_save_mgcreadcounts": { - "type": "boolean", - "description": "Turn on saving the mgc reads count to a separate file." - }, - "motus_output_type": { - "type": "boolean", - "description": "Turn on printing result as counts instead of relative abundances." } }, "fa_icon": "fas fa-align-center" @@ -783,5 +771,19 @@ { "$ref": "#/definitions/reference_genome_options" } - ] + ], + "properties": { + "motus_use_relative_abundance": { + "type": "string", + "default": "false" + }, + "motus_remove_ncbi_ids": { + "type": "string", + "default": "false" + }, + "motus_save_mgc_read_counts": { + "type": "string", + "default": "false" + } + } } From f960c14caf6cf9625f336fbaecad7acfee806d37 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Thu, 1 Dec 2022 13:46:57 +0100 Subject: [PATCH 120/132] Fix typos --- conf/test_krakenuniq.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/test_krakenuniq.config b/conf/test_krakenuniq.config index c504ec9..67b559e 100644 --- a/conf/test_krakenuniq.config +++ b/conf/test_krakenuniq.config @@ -36,8 +36,8 @@ params { perform_longread_hostremoval = true perform_runmerging = true hostremoval_reference = 'https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/genome/genome.fasta' - run_kaiju = false` - run_kraken2 = false` + run_kaiju = false + run_kraken2 = false run_bracken = false run_malt = false run_metaphlan3 = false From 4800a5f03ee654ed2cb16f3b7bf273c29bf95b18 Mon Sep 17 00:00:00 2001 From: sofstam Date: Thu, 1 Dec 2022 14:23:40 +0100 Subject: [PATCH 121/132] Update schema --- nextflow_schema.json | 28 +++++++++++++--------------- 1 file changed, 13 insertions(+), 15 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index e6db686..8d473a9 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -472,6 +472,18 @@ "type": "boolean", "fa_icon": "fas fa-toggle-on", "description": "Turn on profiling with mOTUs. Requires database to be present CSV file passed to --databases" + }, + "motus_use_relative_abundance": { + "type": "boolean", + "description": "Turn on printing relative abundance instead of counts." + }, + "motus_save_mgc_read_counts": { + "type": "boolean", + "description": "Turn on saving the mgc reads count." + }, + "motus_remove_ncbi_ids": { + "type": "boolean", + "description": "Turn on removing NCBI taxonomic IDs." } }, "fa_icon": "fas fa-align-center" @@ -771,19 +783,5 @@ { "$ref": "#/definitions/reference_genome_options" } - ], - "properties": { - "motus_use_relative_abundance": { - "type": "string", - "default": "false" - }, - "motus_remove_ncbi_ids": { - "type": "string", - "default": "false" - }, - "motus_save_mgc_read_counts": { - "type": "string", - "default": "false" - } - } + ] } From 410196c37b740fcc60e382ae3ba7142a2dc7341a Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 1 Dec 2022 14:33:34 +0000 Subject: [PATCH 122/132] [automated] Fix linting with Prettier --- nextflow_schema.json | 55 +++++++------------------------------------- 1 file changed, 8 insertions(+), 47 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 8d473a9..3468f64 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,11 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "databases", - "outdir" - ], + "required": ["input", "databases", "outdir"], "properties": { "input": { "type": "string", @@ -63,10 +59,7 @@ "preprocessing_qc_tool": { "type": "string", "default": "fastqc", - "enum": [ - "fastqc", - "falco" - ], + "enum": ["fastqc", "falco"], "help_text": "Falco is designed as a drop-in replacement for FastQC but written in C++ for faster computation. We particularly recommend using falco when using long reads (due to reduced memory constraints), however is also applicable for short reads.", "description": "Specify the tool used for quality control of raw sequencing reads", "fa_icon": "fas fa-tools" @@ -95,10 +88,7 @@ "shortread_qc_tool": { "type": "string", "default": "fastp", - "enum": [ - "fastp", - "adapterremoval" - ], + "enum": ["fastp", "adapterremoval"], "fa_icon": "fas fa-tools", "description": "Specify which tool to use for short-read QC" }, @@ -157,11 +147,7 @@ "shortread_complexityfilter_tool": { "type": "string", "default": "bbduk", - "enum": [ - "bbduk", - "prinseqplusplus", - "fastp" - ], + "enum": ["bbduk", "prinseqplusplus", "fastp"], "fa_icon": "fas fa-hammer", "description": "Specify which tool to use for complexity filtering" }, @@ -195,10 +181,7 @@ "shortread_complexityfilter_prinseqplusplus_mode": { "type": "string", "default": "entropy", - "enum": [ - "entropy", - "dust" - ], + "enum": ["entropy", "dust"], "fa_icon": "fas fa-check-square", "description": "Specify the complexity filter mode for PRINSEQ++" }, @@ -372,15 +355,7 @@ "diamond_output_format": { "type": "string", "default": "tsv", - "enum": [ - "blast", - "xml", - "txt", - "daa", - "sam", - "tsv", - "paf" - ], + "enum": ["blast", "xml", "txt", "daa", "sam", "tsv", "paf"], "fa_icon": "fas fa-file", "description": "Specify output format from DIAMOND profiling.", "help_text": "DIAMOND can produce output in a number of different formats, you can specify here which to produce.\n\nNote that DIAMOND can only produce one format at a time, and depending on which you pick, some downstream steps may not be executed. For example, selecting `daa` or `sam` will mean you will not get a tabular taxonomic profile as with the other tools.\n\nWill be overriden by `--diamond_save_reads.`\n\n> Modifies tool parameter(s):\n> - diamond blastx: `--outfmt`" @@ -399,14 +374,7 @@ "kaiju_taxon_rank": { "type": "string", "default": "species", - "enum": [ - "phylum", - "class", - "order", - "family", - "genus", - "species" - ], + "enum": ["phylum", "class", "order", "family", "genus", "species"], "fa_icon": "fas fa-tag", "description": "Specify taxonomic rank to be displayed in Kaiju taxon table", "help_text": "Specify the taxonomic level(s) to be displayed in the resulting Kaiju taxon table, as generated by the kaiju2table helper tool.\n\nThis can be either a single level (e.g. `species`), or a comma separated list to display the full taxonomic path (e.g. `superkingdom,phylum,class,order,family,genus,species.`).\n\n> Modifies tool parameter(s):\n> - kaiju2table: `-l`" @@ -624,14 +592,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { From 7e7299203c9e8e21242eb3fd9cc51b913d8f7816 Mon Sep 17 00:00:00 2001 From: Sofia Stamouli <91951607+sofstam@users.noreply.github.com> Date: Fri, 2 Dec 2022 08:55:23 +0100 Subject: [PATCH 123/132] Set values to false --- conf/test_motus.config | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/test_motus.config b/conf/test_motus.config index 29808ed..ee41447 100644 --- a/conf/test_motus.config +++ b/conf/test_motus.config @@ -44,8 +44,8 @@ params { run_diamond = false run_krakenuniq = false run_motus = true - motus_save_mgc_read_counts = true - motus_remove_ncbi_ids = true - motus_use_relative_abundance = true + motus_save_mgc_read_counts = false + motus_remove_ncbi_ids = false + motus_use_relative_abundance = false run_profile_standardisation = true } From de74e4f727ae5c35b12c3203aa1462be4c985037 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Fri, 2 Dec 2022 12:47:14 +0100 Subject: [PATCH 124/132] Fix centrifuge warning, filter out long reads from Brakcen, add some caveat docs to usage.md --- docs/usage.md | 24 ++++++++++++++++++- subworkflows/local/profiling.nf | 10 +++++++- .../local/standardisation_profiles.nf | 10 ++++---- 3 files changed, 37 insertions(+), 7 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index a87102c..d21761f 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -248,9 +248,31 @@ You can optionally save the FASTQ output of the run merging with the `--save_run ##### Profiling +###### Bracken + +It is unclear whether Bracken is suitable for running long reads, as it makes certain assumptions about read lengths. Furthemore, during testing we found issues where Bracken would fail on the long-read test data. Therefore nf-core/taxprofiler does not run Bracken on data specified as being sequenced with `OXFORD_NANOPORE` in the input samplesheet. If you believe this to be wrong, please contact us on the nf-core slack and we can discuss this. + +###### Centrifuge + +Centrifuge currently does not accept FASTA files as input, therefore no output will be produced for these input files. + +###### DIAMOND + +DIAMOND only allows output of a single format at a time, therefore parameters such --diamond_save_reads supplied will result in only aligned reads in SAM format will be produced, no taxonomic profiles will be available. Be aware of this when setting up your pipeline runs, depending n your particular use case. + ###### MALT -nf-core/taxprofiler uses MALT 0.4.1, which is a compatively old version. However it has been found that the most recent version of MALT (0.5.\*), at the time of writing, is broken. [The LCA step appears not to be executed](http://megan.informatik.uni-tuebingen.de/t/lca-placement-failure-with-malt-v-0-5-2-and-0-5-3/1996/3), pushing all hits to the leaves of the taxonomy. However, if you need to use a more recent taxonomy map file with your databases, the output of `malt-build` from MALT 0.5.3 should be still be compatible with `malt-run` of 0.4.1. +MALT does not support paired-end reads alignment (unlike other tools), therefore nf-core/taxprofiler aligns these as indepenent files if read-merging is skipped. If you skip merging, you can sum or average the results of the counts of the pairs. + +Krona can only be run on MALT output if path to Krona taxonomy database supplied to `--krona_taxonomy_directory`. Therefore if you do not supply the a KRona directory, Krona plots will not be produced for MALT. + +###### MetaPhlAn3 + +MetaPhlAn3 currently does not accept FASTA files as input, therefore no output will be produced for these input files. + +###### mOTUs + +mOTUs currently does not accept FASTA files as input, therefore no output will be produced for these input files. ### Updating the pipeline diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index aeee2a4..5256951 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -145,7 +145,15 @@ workflow PROFILING { if ( params.run_kraken2 && params.run_bracken ) { // Remove files from 'pure' kraken2 runs, so only those aligned against Bracken & kraken2 database are used. def ch_kraken2_output = KRAKEN2_KRAKEN2.out.report - .filter { meta, report -> meta['tool'] == 'bracken' } + .filter { + meta, report -> + if ( meta['instrument_platform'] == 'OXFORD_NANOPORE' ) log.warn "[nf-core/taxprofiler] Bracken has not been evaluated for long-read data. Skipping Bracken for sample ${meta.id}." + meta['tool'] == 'bracken' && meta['instrument_platform'] != 'OXFORD_NANOPORE' + + + } + + // If necessary, convert the eight column output to six column output. if (params.kraken2_save_minimizers) { diff --git a/subworkflows/local/standardisation_profiles.nf b/subworkflows/local/standardisation_profiles.nf index cbb0fab..8c73472 100644 --- a/subworkflows/local/standardisation_profiles.nf +++ b/subworkflows/local/standardisation_profiles.nf @@ -3,7 +3,7 @@ // include { KAIJU_KAIJU2TABLE } from '../../modules/nf-core/kaiju/kaiju2table/main' -include { KRAKENTOOLS_COMBINEKREPORTS } from '../../modules/nf-core/krakentools/combinekreports/main' +include { KRAKENTOOLS_COMBINEKREPORTS as KRAKENTOOLS_COMBINEKREPORTS_KRAKEN } from '../../modules/nf-core/krakentools/combinekreports/main' include { KRAKENTOOLS_COMBINEKREPORTS as KRAKENTOOLS_COMBINEKREPORTS_CENTRIFUGE } from '../../modules/nf-core/krakentools/combinekreports/main' include { METAPHLAN3_MERGEMETAPHLANTABLES } from '../../modules/nf-core/metaphlan3/mergemetaphlantables/main' include { MOTUS_MERGE } from '../../modules/nf-core/motus/merge/main' @@ -93,10 +93,10 @@ workflow STANDARDISATION_PROFILES { [[id:it[0]], it[1]] } - KRAKENTOOLS_COMBINEKREPORTS ( ch_profiles_for_kraken2 ) - ch_standardised_tables = ch_standardised_tables.mix( KRAKENTOOLS_COMBINEKREPORTS.out.txt ) - ch_multiqc_files = ch_multiqc_files.mix( KRAKENTOOLS_COMBINEKREPORTS.out.txt ) - ch_versions = ch_versions.mix( KRAKENTOOLS_COMBINEKREPORTS.out.versions ) + KRAKENTOOLS_COMBINEKREPORTS_KRAKEN ( ch_profiles_for_kraken2 ) + ch_standardised_tables = ch_standardised_tables.mix( KRAKENTOOLS_COMBINEKREPORTS_KRAKEN.out.txt ) + ch_multiqc_files = ch_multiqc_files.mix( KRAKENTOOLS_COMBINEKREPORTS_KRAKEN.out.txt ) + ch_versions = ch_versions.mix( KRAKENTOOLS_COMBINEKREPORTS_KRAKEN.out.versions ) // MetaPhlAn3 From ab42f6b66272240223ee7807ad08c5b8ac9c7b1c Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Fri, 2 Dec 2022 12:49:00 +0100 Subject: [PATCH 125/132] wteak message --- subworkflows/local/profiling.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index b8eb086..ec15386 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -147,7 +147,7 @@ workflow PROFILING { def ch_kraken2_output = KRAKEN2_KRAKEN2.out.report .filter { meta, report -> - if ( meta['instrument_platform'] == 'OXFORD_NANOPORE' ) log.warn "[nf-core/taxprofiler] Bracken has not been evaluated for long-read data. Skipping Bracken for sample ${meta.id}." + if ( meta['instrument_platform'] == 'OXFORD_NANOPORE' ) log.warn "[nf-core/taxprofiler] Bracken has not been evaluated for Nanopore data. Skipping Bracken for sample ${meta.id}." meta['tool'] == 'bracken' && meta['instrument_platform'] != 'OXFORD_NANOPORE' From cf8ad9677124c57e8ade33d5d56de719e6eb9e5b Mon Sep 17 00:00:00 2001 From: "James A. Fellows Yates" Date: Fri, 2 Dec 2022 15:09:08 +0100 Subject: [PATCH 126/132] Update subworkflows/local/profiling.nf --- subworkflows/local/profiling.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index ec15386..2454ef3 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -147,8 +147,8 @@ workflow PROFILING { def ch_kraken2_output = KRAKEN2_KRAKEN2.out.report .filter { meta, report -> - if ( meta['instrument_platform'] == 'OXFORD_NANOPORE' ) log.warn "[nf-core/taxprofiler] Bracken has not been evaluated for Nanopore data. Skipping Bracken for sample ${meta.id}." meta['tool'] == 'bracken' && meta['instrument_platform'] != 'OXFORD_NANOPORE' + if ( meta['instrument_platform'] == 'OXFORD_NANOPORE' ) log.warn "[nf-core/taxprofiler] Bracken has not been evaluated for Nanopore data. Skipping Bracken for sample ${meta.id}." } From 0dccfddc249107e69f3dfdb748d304a0e2aaa676 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Sat, 3 Dec 2022 21:38:25 +0100 Subject: [PATCH 127/132] Switch order back --- subworkflows/local/profiling.nf | 6 +----- 1 file changed, 1 insertion(+), 5 deletions(-) diff --git a/subworkflows/local/profiling.nf b/subworkflows/local/profiling.nf index 2454ef3..f5c970c 100644 --- a/subworkflows/local/profiling.nf +++ b/subworkflows/local/profiling.nf @@ -147,14 +147,10 @@ workflow PROFILING { def ch_kraken2_output = KRAKEN2_KRAKEN2.out.report .filter { meta, report -> - meta['tool'] == 'bracken' && meta['instrument_platform'] != 'OXFORD_NANOPORE' if ( meta['instrument_platform'] == 'OXFORD_NANOPORE' ) log.warn "[nf-core/taxprofiler] Bracken has not been evaluated for Nanopore data. Skipping Bracken for sample ${meta.id}." - - + meta['tool'] == 'bracken' && meta['instrument_platform'] != 'OXFORD_NANOPORE' } - - // If necessary, convert the eight column output to six column output. if (params.kraken2_save_minimizers) { ch_kraken2_output = KRAKEN2_STANDARD_REPORT(ch_kraken2_output).report From 0a0dffa37ca581386d17cb9dd4296e18697196f4 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Sun, 4 Dec 2022 12:06:33 +0100 Subject: [PATCH 128/132] Try separating MALT --- .github/workflows/ci.yml | 33 +++++++++++++++++++++++++++++++++ conf/test.config | 2 +- 2 files changed, 34 insertions(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index d179c6e..dad2838 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -134,3 +134,36 @@ jobs: with: command: nextflow run ${GITHUB_WORKSPACE} -profile test_krakenuniq,docker --outdir ./results attempt_limit: 3 + + malt: + name: Test MALT with workflow parameters + if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/taxprofiler') }} + runs-on: ubuntu-latest + strategy: + matrix: + NXF_VER: + - "21.10.3" + - "latest-everything" + + steps: + - name: Check out pipeline code + uses: actions/checkout@v2 + + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + with: + version: "${{ matrix.NXF_VER }}" + + - name: Show current locale + run: locale + + - name: Set UTF-8 enabled locale + run: | + sudo locale-gen en_US.UTF-8 + sudo update-locale LANG=en_US.UTF-8 + + - name: Run pipeline with test data + uses: Wandalen/wretry.action@v1.0.11 + with: + command: nextflow run ${GITHUB_WORKSPACE} -profile test_nothing,docker --run_malt --outdir ./results + attempt_limit: 3 diff --git a/conf/test.config b/conf/test.config index 898743e..db9f81d 100644 --- a/conf/test.config +++ b/conf/test.config @@ -35,7 +35,7 @@ params { run_kaiju = true run_kraken2 = true run_bracken = true - run_malt = true + run_malt = false run_metaphlan3 = true run_centrifuge = true run_diamond = true From 4529e7e5457df2de7557c6e8d7c1295f3c24daa7 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Thu, 1 Dec 2022 17:25:25 +0100 Subject: [PATCH 129/132] refactor: change parameter exclude unmerged to include --- .github/workflows/ci.yml | 4 ++-- conf/modules.config | 2 +- docs/usage.md | 2 +- nextflow.config | 2 +- nextflow_schema.json | 6 +++--- subworkflows/local/shortread_adapterremoval.nf | 4 ++-- workflows/taxprofiler.nf | 2 +- 7 files changed, 11 insertions(+), 11 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index dad2838..11db52e 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,10 +28,10 @@ jobs: - "--perform_longread_qc false" - "--perform_shortread_qc false" - "--shortread_qc_tool fastp" - - "--shortread_qc_tool fastp --shortread_qc_mergepairs --shortread_qc_excludeunmerged" + - "--shortread_qc_tool fastp --shortread_qc_mergepairs --shortread_qc_includeunmerged" - "--shortread_qc_tool fastp --shortread_qc_mergepairs" - "--shortread_qc_tool adapterremoval" - - "--shortread_qc_tool adapterremoval --shortread_qc_mergepairs --shortread_qc_excludeunmerged" + - "--shortread_qc_tool adapterremoval --shortread_qc_mergepairs --shortread_qc_includeunmerged" - "--shortread_qc_tool adapterremoval --shortread_qc_mergepairs" - "--shortread_complexityfilter_tool bbduk" - "--shortread_complexityfilter_tool prinseqplusplus" diff --git a/conf/modules.config b/conf/modules.config index f6045e5..dd85c0c 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -79,7 +79,7 @@ process { withName: FASTP_PAIRED { ext.args = [ // collapsing options - option to retain singletons - params.shortread_qc_excludeunmerged ? '' : "--include_unmerged", + params.shortread_qc_includeunmerged ? '--include_unmerged' : '', // trimming options params.shortread_qc_skipadaptertrim ? "--disable_adapter_trimming" : "", params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter1 ? "--adapter_sequence ${params.shortread_qc_adapter1}" : "", diff --git a/docs/usage.md b/docs/usage.md index d21761f..d2f41bf 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -204,7 +204,7 @@ It is highly recommended to run this on raw reads to remove artifacts from seque There are currently two options for short-read preprocessing: `fastp` or `adapterremoval`. For adapter clipping, you can either rely on tool default adapter sequences, or supply your own adapters (`--shortread_qc_adapter1` and `--shortread_qc_adapter2`) -By default, paired-end merging is not activated and paired-end profiling is performed where supported otherwise pairs will be independently profiled. If paired-end merging is activated you can also specify whether to exclude unmerged reads in the reads sent for profiling (`--shortread_qc_mergepairs` and `--shortread_qc_excludeunmerged`). +By default, paired-end merging is not activated and paired-end profiling is performed where supported otherwise pairs will be independently profiled. If paired-end merging is activated you can also specify whether to include unmerged reads in the reads sent for profiling (`--shortread_qc_mergepairs` and `--shortread_qc_includeunmerged`). You can also turn off clipping and only perform paired-end merging, if requested. This can be useful when processing data downloaded from the ENA, SRA, or DDBJ (`--shortread_qc_skipadaptertrim`). Both tools support length filtering of reads and can be tuned with `--shortread_qc_minlength`. Performing length filtering can be useful to remove short (often low sequencing complexity) sequences that result in unspecific classification and therefore slow down runtime during profiling, with minimal gain. diff --git a/nextflow.config b/nextflow.config index 3cf160d..54e220d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -66,7 +66,7 @@ params { shortread_qc_tool = 'fastp' shortread_qc_skipadaptertrim = false shortread_qc_mergepairs = true - shortread_qc_excludeunmerged = false + shortread_qc_includeunmerged = false shortread_qc_adapter1 = null shortread_qc_adapter2 = null shortread_qc_adapterlist = null diff --git a/nextflow_schema.json b/nextflow_schema.json index ae4e311..350830c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -125,11 +125,11 @@ "default": true, "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" }, - "shortread_qc_excludeunmerged": { + "shortread_qc_includeunmerged": { "type": "boolean", "fa_icon": "far fa-times-circle", - "description": "Discard unmerged reads from paired-end merging", - "help_text": "Turns off the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nThis can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - removed from reads `--include_unmerged`\n" + "description": "Include unmerged reads from paired-end merging in the downstream processing", + "help_text": "Turns on the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nExcluding unmerged reads can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - adds option `--include_unmerged`\n" }, "shortread_qc_minlength": { "type": "integer", diff --git a/subworkflows/local/shortread_adapterremoval.nf b/subworkflows/local/shortread_adapterremoval.nf index 6567007..4340e0d 100644 --- a/subworkflows/local/shortread_adapterremoval.nf +++ b/subworkflows/local/shortread_adapterremoval.nf @@ -30,7 +30,7 @@ workflow SHORTREAD_ADAPTERREMOVAL { * has to be exported in a separate channel and we must manually recombine when necessary. */ - if ( params.shortread_qc_mergepairs && !params.shortread_qc_excludeunmerged ) { + if ( params.shortread_qc_mergepairs && params.shortread_qc_includeunmerged ) { ch_concat_fastq = Channel.empty() .mix( @@ -55,7 +55,7 @@ workflow SHORTREAD_ADAPTERREMOVAL { ch_adapterremoval_reads_prepped = CAT_FASTQ.out.reads .mix(ADAPTERREMOVAL_SINGLE.out.singles_truncated) - } else if ( params.shortread_qc_mergepairs && params.shortread_qc_excludeunmerged ) { + } else if ( params.shortread_qc_mergepairs && !params.shortread_qc_includeunmerged ) { ch_concat_fastq = Channel.empty() .mix( diff --git a/workflows/taxprofiler.nf b/workflows/taxprofiler.nf index ba52a4f..68fc47a 100644 --- a/workflows/taxprofiler.nf +++ b/workflows/taxprofiler.nf @@ -28,7 +28,7 @@ if ( params.input ) { if (params.databases) { ch_databases = file(params.databases) } else { exit 1, 'Input database sheet not specified!' } if (params.shortread_qc_mergepairs && params.run_malt ) log.warn "[nf-core/taxprofiler] MALT does not accept uncollapsed paired-reads. Pairs will be profiled as separate files." -if (params.shortread_qc_excludeunmerged && !params.shortread_qc_mergepairs) exit 1, "ERROR: [nf-core/taxprofiler] cannot include unmerged reads when merging not turned on. Please specify --shortread_qc_mergepairs" +if (params.shortread_qc_includeunmerged && !params.shortread_qc_mergepairs) exit 1, "ERROR: [nf-core/taxprofiler] cannot include unmerged reads when merging is not turned on. Please specify --shortread_qc_mergepairs" if (params.shortread_complexityfilter_tool == 'fastp' && ( params.perform_shortread_qc == false || params.shortread_qc_tool != 'fastp' )) exit 1, "ERROR: [nf-core/taxprofiler] cannot use fastp complexity filtering if preprocessing not turned on and/or tool is not fastp. Please specify --perform_shortread_qc and/or --shortread_qc_tool 'fastp'" From b2088bcbb3bfc1057543df056783dcb3defdfd4a Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Sun, 4 Dec 2022 21:53:38 +0100 Subject: [PATCH 130/132] docs: improve description of `shortread_qc_includeunmerged` --- nextflow_schema.json | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 350830c..02085d5 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -128,8 +128,8 @@ "shortread_qc_includeunmerged": { "type": "boolean", "fa_icon": "far fa-times-circle", - "description": "Include unmerged reads from paired-end merging in the downstream processing", - "help_text": "Turns on the inclusion of unmerged reads in resulting processing FASTQ file of paired-end sequencing data when using `fastp`.\n\nExcluding unmerged reads can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Modifies tool parameter(s):\n> - adds option `--include_unmerged`\n" + "description": "Include unmerged reads from paired-end merging in the downstream analysis", + "help_text": "Turns on the inclusion of unmerged reads in resulting FASTQ file from merging paired-end sequencing data when using `fastp` and/or `AdapterRemoval`. For `fastp` this means the unmerged read pairs are directly included in the output FASTQ file. For `AdapterRemoval`, additional output files containing unmerged reads are all concatenated into one file by the workflow.\n\nExcluding unmerged reads can be useful in cases where you prefer to have very short reads (e.g. aDNA), thus excluding longer-reads or possibly faulty reads where one of the pair was discarded.\n\n> Adds `fastp` option: `--include_unmerged`\n" }, "shortread_qc_minlength": { "type": "integer", From 24e5bc54101873f86a5e64610162769651ba8f26 Mon Sep 17 00:00:00 2001 From: "Moritz E. Beber" Date: Sun, 4 Dec 2022 21:54:10 +0100 Subject: [PATCH 131/132] refactor: use shorter and more explicit syntax --- subworkflows/local/shortread_adapterremoval.nf | 8 ++------ 1 file changed, 2 insertions(+), 6 deletions(-) diff --git a/subworkflows/local/shortread_adapterremoval.nf b/subworkflows/local/shortread_adapterremoval.nf index 4340e0d..7f5a0fb 100644 --- a/subworkflows/local/shortread_adapterremoval.nf +++ b/subworkflows/local/shortread_adapterremoval.nf @@ -40,9 +40,7 @@ workflow SHORTREAD_ADAPTERREMOVAL { ADAPTERREMOVAL_PAIRED.out.paired_truncated ) .map { meta, reads -> - def meta_new = meta.clone() - meta_new.single_end = true - [meta_new, reads] + [meta + [single_end: true], reads] } .groupTuple() // Paired-end reads cause a nested tuple during grouping. @@ -63,9 +61,7 @@ workflow SHORTREAD_ADAPTERREMOVAL { ADAPTERREMOVAL_PAIRED.out.collapsed_truncated ) .map { meta, reads -> - def meta_new = meta.clone() - meta_new.single_end = true - [meta_new, reads] + [meta + [single_end: true], reads] } .groupTuple() .map { meta, fastq -> [meta, fastq.flatten()] } From 3dba6405c53939a81ec3e82569d9c7ec7290e0c0 Mon Sep 17 00:00:00 2001 From: James Fellows Yates Date: Tue, 6 Dec 2022 11:47:16 +0100 Subject: [PATCH 132/132] Sets correct default for --shortread_qc_mergepairs --- nextflow.config | 2 +- nextflow_schema.json | 3 +-- 2 files changed, 2 insertions(+), 3 deletions(-) diff --git a/nextflow.config b/nextflow.config index 54e220d..00edd9b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -65,7 +65,7 @@ params { perform_shortread_qc = false shortread_qc_tool = 'fastp' shortread_qc_skipadaptertrim = false - shortread_qc_mergepairs = true + shortread_qc_mergepairs = false shortread_qc_includeunmerged = false shortread_qc_adapter1 = null shortread_qc_adapter2 = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 02085d5..d6debb3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -122,8 +122,7 @@ "type": "boolean", "fa_icon": "fas fa-toggle-on", "description": "Turn on merging of read pairs for paired-end data", - "default": true, - "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data for AdapterRemoval (this is performed automatically with fastp).\n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n" + "help_text": "Turn on the merging of read-pairs of paired-end short read sequencing data. \n\n> Modifies tool parameter(s):\n> - AdapterRemoval: `--collapse`\n> - fastp: `-m --merged_out`\n" }, "shortread_qc_includeunmerged": { "type": "boolean",