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include krakenuniq in profiling subworkflow
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36e101a78b
commit
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2 changed files with 22 additions and 0 deletions
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@ -89,6 +89,10 @@
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"branch": "master",
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"git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905"
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},
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"krakenuniq/preloadedkrakenuniq": {
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"branch": "master",
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"git_sha": "13b9d4854593c03e5e25e8a8f47462542c2c0dd4"
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},
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"krona/ktimporttaxonomy": {
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"branch": "master",
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"git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905"
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@ -11,6 +11,7 @@ include { METAPHLAN3_METAPHLAN3 } from '../../modules/nf-core/me
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include { KAIJU_KAIJU } from '../../modules/nf-core/kaiju/kaiju/main'
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include { DIAMOND_BLASTX } from '../../modules/nf-core/diamond/blastx/main'
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include { MOTUS_PROFILE } from '../../modules/nf-core/motus/profile/main'
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include { KRAKENUNIQ_PRELOADEDKRAKENUNIQ } from '../../modules/nf-core/krakenuniq/preloadedkrakenuniq/main'
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workflow PROFILING {
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take:
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@ -45,6 +46,7 @@ workflow PROFILING {
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kaiju: it[2]['tool'] == 'kaiju'
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diamond: it[2]['tool'] == 'diamond'
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motus: it[2]['tool'] == 'motus'
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krakenuniq: it[2]['tool'] == 'krakenuniq'
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unknown: true
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}
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@ -228,6 +230,22 @@ workflow PROFILING {
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ch_multiqc_files = ch_multiqc_files.mix( MOTUS_PROFILE.out.log )
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}
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if ( params.run_krakenuniq ) {
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ch_input_for_krakenuniq = ch_input_for_profiling.krakenuniq
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.multiMap {
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it ->
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reads: [ it[0] + it[2], it[1] ]
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db: it[3]
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}
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KRAKENUNIQ_PRELOADEDKRAKENUNIQ ( ch_input_for_krakenuniq.reads, ch_input_for_krakenuniq.db, params.krakenuniq_save_reads, params.krakenuniq_save_readclassification )
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ch_multiqc_files = ch_multiqc_files.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.report )
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ch_versions = ch_versions.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.versions.first() )
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ch_raw_classifications = ch_raw_classifications.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.classified_assignment )
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ch_raw_profiles = ch_raw_profiles.mix( KRAKENUNIQ_PRELOADEDKRAKENUNIQ.out.report )
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}
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emit:
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classifications = ch_raw_classifications
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profiles = ch_raw_profiles // channel: [ val(meta), [ reads ] ] - should be text files or biom
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