diff --git a/docs/output.md b/docs/output.md
index 2fae37a..ef791e1 100644
--- a/docs/output.md
+++ b/docs/output.md
@@ -64,6 +64,8 @@ fastp can automatically detect adapter sequences for Illumina data.
Output files
- `fastp`
+ - `.fastp.fastq.gz`: File with the trimmed unmerged fastq reads.
+ - `.merged.fastq.gz`: File with the reads that were successfully merged.
@@ -95,7 +97,7 @@ Note that the FASTQ files may _not_ always be the 'final' reads that go into tax
Output files
- `porechop`
- - `.fastq.gz`
+ - `.fastq.gz`: Adapter-trimmed file
@@ -143,8 +145,8 @@ Note that the FASTQ file(s) may _not_ always be the 'final' reads that go into t
Output files
- `filtlong`
- - `_filtered.fastq.g`
- - `_filtered.log`
+ - `_filtered.fastq.gz`: Quality or short read data filtered file
+ - `_filtered.log`: log file containing summary statistics
@@ -174,7 +176,7 @@ Note that the FASTQ file(s) may _not_ always be the 'final' reads that go into t
Output files
- `minimap2`
- - `.bam`
+ - `.bam`: Alignment file in bam format
@@ -184,7 +186,7 @@ Note that the FASTQ file(s) may _not_ always be the 'final' reads that go into t
Output files
- `samtoolsstats`
- - `.stats`
+ - `.stats`: File containing samtools stats output
@@ -204,7 +206,7 @@ Note that the FASTQ file(s) may _not_ always be the 'final' reads that go into t
-The main taxonomic profiling file from KrakenUniq is the `*.tsv` file. This provides the basic results from Kraken2 but with the corrected abundance information.
+The main taxonomic profiling file from KrakenUniq is the `*.tsv` file. This provides the basic results from Kraken2 but with the corrected abundance information.
### Kraken2
@@ -256,10 +258,11 @@ You will only receive the FASTQs and `*classifiedreads.txt` file if you supply `
Output files
- `centrifuge`
- - `.centrifuge.mapped.fastq.gz`
- - `.centrifuge.report.txt`
- - `.centrifuge.results.txt`
- - `.centrifuge.unmapped.fastq.gz`
+ - `.centrifuge.mapped.fastq.gz`: Fastq files containing all mapped reads
+ - `.centrifuge.report.txt`: A classification report that summarises the taxonomic ID, the taxonomic rank, length of genome sequence, number of classified and uniquely classified reads
+ - `.centrifuge.results.txt`: A file that summarises the classification assignment for a read, i.e read ID, sequence ID, score for the classification, score for the next best classification, number of classifications for this read
+ - `.centrifuge.txt`: A Kraken2-style report that summarises the fraction abundance, taxonomic ID, number of k-mers, taxonomic path of all the hits in the centrifuge run for a given sample
+ - `.centrifuge.unmapped.fastq.gz`: Fastq file containing all unmapped reads
@@ -269,7 +272,8 @@ You will only receive the FASTQs and `*classifiedreads.txt` file if you supply `
Output files
- `kaiju`
- - `.tsv`
+ - `.tsv`: A file that summarises the fraction abundance, taxonomic ID, number of reads and taxonomic names
+ - `kaiju__combined_reports.txt`: A combined profile of all samples aligned to a given database (as generated by `kaiju2table`)
@@ -279,8 +283,8 @@ You will only receive the FASTQs and `*classifiedreads.txt` file if you supply `
Output files
- `diamond`
- - `.log`
- - `.sam`
+ - `.log`: A log file containing stdout information
+ - `.sam`: A file in SAM format that contains the aligned reads
@@ -321,22 +325,23 @@ You will only recieve the `.sam` and `.megan` files if you supply `--malt_save_r
-The main taxonomic profiling file from MetaPhlAn3 is the `*_profile.txt` file. This provides the abundance estimates from MetaPhlAn3 however does not include raw counts by default.
+The main taxonomic profiling file from MetaPhlAn3 is the `*_profile.txt` file. This provides the abundance estimates from MetaPhlAn3 however does not include raw counts by default.
### mOTUs
Output files
- `motus`
- - `.log`
- - `.out`
+ - `.log`: A log file that contains summary statistics
+ - `.out`: A classification file that summarises taxonomic identifiers, by default at the rank of mOTUs (i.e., species level), and their relative abundances in the profiled sample.
+ - `motus__combined_reports.txt`: A combined profile of all samples aligned to a given database (as generated by `motus_merge`)
### Krona
[Krona](https://github.com/marbl/Krona) is Krona allows the exploration of (metagenomic) hierarchical data with interactive zooming, multi-layered pie charts.
-Krona charts will be generated by the pipeline for supported tools (Kraken2, Centrifuge, Kaiju, and MALT)
+Krona charts will be generated by the pipeline for supported tools (Kraken2, Centrifuge, Kaiju, and MALT)
Output files