mirror of
https://github.com/MillironX/taxprofiler.git
synced 2024-11-22 08:59:55 +00:00
Merge pull request #19 from nf-core/nf-core-template-merge-2.3
Important! Template update for nf-core/tools v2.3
This commit is contained in:
commit
9fac82d342
25 changed files with 193 additions and 139 deletions
1
.github/ISSUE_TEMPLATE/bug_report.yml
vendored
1
.github/ISSUE_TEMPLATE/bug_report.yml
vendored
|
@ -1,4 +1,3 @@
|
||||||
|
|
||||||
name: Bug report
|
name: Bug report
|
||||||
description: Report something that is broken or incorrect
|
description: Report something that is broken or incorrect
|
||||||
labels: bug
|
labels: bug
|
||||||
|
|
2
.github/PULL_REQUEST_TEMPLATE.md
vendored
2
.github/PULL_REQUEST_TEMPLATE.md
vendored
|
@ -19,7 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/taxp
|
||||||
- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/taxprofiler/tree/master/.github/CONTRIBUTING.md)
|
- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/taxprofiler/tree/master/.github/CONTRIBUTING.md)
|
||||||
- [ ] If necessary, also make a PR on the nf-core/taxprofiler _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
|
- [ ] If necessary, also make a PR on the nf-core/taxprofiler _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
|
||||||
- [ ] Make sure your code lints (`nf-core lint`).
|
- [ ] Make sure your code lints (`nf-core lint`).
|
||||||
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker`).
|
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker` --outdir <OUTDIR>`).
|
||||||
- [ ] Usage Documentation in `docs/usage.md` is updated.
|
- [ ] Usage Documentation in `docs/usage.md` is updated.
|
||||||
- [ ] Output Documentation in `docs/output.md` is updated.
|
- [ ] Output Documentation in `docs/output.md` is updated.
|
||||||
- [ ] `CHANGELOG.md` is updated.
|
- [ ] `CHANGELOG.md` is updated.
|
||||||
|
|
6
.github/workflows/awsfulltest.yml
vendored
6
.github/workflows/awsfulltest.yml
vendored
|
@ -14,7 +14,7 @@ jobs:
|
||||||
runs-on: ubuntu-latest
|
runs-on: ubuntu-latest
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||||||
steps:
|
steps:
|
||||||
- name: Launch workflow via tower
|
- name: Launch workflow via tower
|
||||||
uses: nf-core/tower-action@v2
|
uses: nf-core/tower-action@v3
|
||||||
# TODO nf-core: You can customise AWS full pipeline tests as required
|
# TODO nf-core: You can customise AWS full pipeline tests as required
|
||||||
# Add full size test data (but still relatively small datasets for few samples)
|
# Add full size test data (but still relatively small datasets for few samples)
|
||||||
# on the `test_full.config` test runs with only one set of parameters
|
# on the `test_full.config` test runs with only one set of parameters
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||||||
|
@ -31,4 +31,6 @@ jobs:
|
||||||
"outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/taxprofiler/results-${{ github.sha }}"
|
"outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/taxprofiler/results-${{ github.sha }}"
|
||||||
}
|
}
|
||||||
profiles: test_full,aws_tower
|
profiles: test_full,aws_tower
|
||||||
pre_run_script: 'export NXF_VER=21.10.3'
|
nextflow_config: |
|
||||||
|
process.errorStrategy = 'retry'
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||||||
|
process.maxRetries = 3
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||||||
|
|
6
.github/workflows/awstest.yml
vendored
6
.github/workflows/awstest.yml
vendored
|
@ -11,7 +11,7 @@ jobs:
|
||||||
runs-on: ubuntu-latest
|
runs-on: ubuntu-latest
|
||||||
steps:
|
steps:
|
||||||
- name: Launch workflow via tower
|
- name: Launch workflow via tower
|
||||||
uses: nf-core/tower-action@v2
|
uses: nf-core/tower-action@v3
|
||||||
|
|
||||||
with:
|
with:
|
||||||
workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
|
workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
|
||||||
|
@ -25,4 +25,6 @@ jobs:
|
||||||
"outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/taxprofiler/results-test-${{ github.sha }}"
|
"outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/taxprofiler/results-test-${{ github.sha }}"
|
||||||
}
|
}
|
||||||
profiles: test,aws_tower
|
profiles: test,aws_tower
|
||||||
pre_run_script: 'export NXF_VER=21.10.3'
|
nextflow_config: |
|
||||||
|
process.errorStrategy = 'retry'
|
||||||
|
process.maxRetries = 3
|
||||||
|
|
4
.github/workflows/ci.yml
vendored
4
.github/workflows/ci.yml
vendored
|
@ -14,7 +14,7 @@ env:
|
||||||
|
|
||||||
jobs:
|
jobs:
|
||||||
test:
|
test:
|
||||||
name: Run workflow tests
|
name: Run pipeline with test data
|
||||||
# Only run on push if this is the nf-core dev branch (merged PRs)
|
# Only run on push if this is the nf-core dev branch (merged PRs)
|
||||||
if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/taxprofiler') }}
|
if: ${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/taxprofiler') }}
|
||||||
runs-on: ubuntu-latest
|
runs-on: ubuntu-latest
|
||||||
|
@ -47,4 +47,4 @@ jobs:
|
||||||
# For example: adding multiple test runs with different parameters
|
# For example: adding multiple test runs with different parameters
|
||||||
# Remember that you can parallelise this by using strategy.matrix
|
# Remember that you can parallelise this by using strategy.matrix
|
||||||
run: |
|
run: |
|
||||||
nextflow run ${GITHUB_WORKSPACE} -profile test,docker
|
nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results
|
||||||
|
|
28
.github/workflows/linting.yml
vendored
28
.github/workflows/linting.yml
vendored
|
@ -12,9 +12,7 @@ jobs:
|
||||||
runs-on: ubuntu-latest
|
runs-on: ubuntu-latest
|
||||||
steps:
|
steps:
|
||||||
- uses: actions/checkout@v2
|
- uses: actions/checkout@v2
|
||||||
- uses: actions/setup-node@v1
|
- uses: actions/setup-node@v2
|
||||||
with:
|
|
||||||
node-version: '10'
|
|
||||||
- name: Install markdownlint
|
- name: Install markdownlint
|
||||||
run: npm install -g markdownlint-cli
|
run: npm install -g markdownlint-cli
|
||||||
- name: Run Markdownlint
|
- name: Run Markdownlint
|
||||||
|
@ -51,9 +49,7 @@ jobs:
|
||||||
steps:
|
steps:
|
||||||
- uses: actions/checkout@v2
|
- uses: actions/checkout@v2
|
||||||
|
|
||||||
- uses: actions/setup-node@v1
|
- uses: actions/setup-node@v2
|
||||||
with:
|
|
||||||
node-version: '10'
|
|
||||||
|
|
||||||
- name: Install editorconfig-checker
|
- name: Install editorconfig-checker
|
||||||
run: npm install -g editorconfig-checker
|
run: npm install -g editorconfig-checker
|
||||||
|
@ -64,14 +60,13 @@ jobs:
|
||||||
YAML:
|
YAML:
|
||||||
runs-on: ubuntu-latest
|
runs-on: ubuntu-latest
|
||||||
steps:
|
steps:
|
||||||
- uses: actions/checkout@v1
|
- name: Checkout
|
||||||
- uses: actions/setup-node@v1
|
uses: actions/checkout@master
|
||||||
|
- name: 'Yamllint'
|
||||||
|
uses: karancode/yamllint-github-action@master
|
||||||
with:
|
with:
|
||||||
node-version: '10'
|
yamllint_file_or_dir: '.'
|
||||||
- name: Install yaml-lint
|
yamllint_config_filepath: '.yamllint.yml'
|
||||||
run: npm install -g yaml-lint
|
|
||||||
- name: Run yaml-lint
|
|
||||||
run: yamllint $(find ${GITHUB_WORKSPACE} -type f -name "*.yml" -o -name "*.yaml")
|
|
||||||
|
|
||||||
# If the above check failed, post a comment on the PR explaining the failure
|
# If the above check failed, post a comment on the PR explaining the failure
|
||||||
- name: Post PR comment
|
- name: Post PR comment
|
||||||
|
@ -84,10 +79,11 @@ jobs:
|
||||||
To keep the code consistent with lots of contributors, we run automated code consistency checks.
|
To keep the code consistent with lots of contributors, we run automated code consistency checks.
|
||||||
To fix this CI test, please run:
|
To fix this CI test, please run:
|
||||||
|
|
||||||
* Install `yaml-lint`
|
* Install `yamllint`
|
||||||
* [Install `npm`](https://www.npmjs.com/get-npm) then [install `yaml-lint`](https://www.npmjs.com/package/yaml-lint) (`npm install -g yaml-lint`)
|
* Install `yamllint` following [this](https://yamllint.readthedocs.io/en/stable/quickstart.html#installing-yamllint)
|
||||||
|
instructions or alternative install it in your [conda environment](https://anaconda.org/conda-forge/yamllint)
|
||||||
* Fix the markdown errors
|
* Fix the markdown errors
|
||||||
* Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml")`
|
* Run the test locally: `yamllint $(find . -type f -name "*.yml" -o -name "*.yaml") -c ./.yamllint.yml`
|
||||||
* Fix any reported errors in your YAML files
|
* Fix any reported errors in your YAML files
|
||||||
|
|
||||||
Once you push these changes the test should pass, and you can hide this comment :+1:
|
Once you push these changes the test should pass, and you can hide this comment :+1:
|
||||||
|
|
14
.gitpod.yml
Normal file
14
.gitpod.yml
Normal file
|
@ -0,0 +1,14 @@
|
||||||
|
image: nfcore/gitpod:latest
|
||||||
|
|
||||||
|
vscode:
|
||||||
|
extensions: # based on nf-core.nf-core-extensionpack
|
||||||
|
- codezombiech.gitignore # Language support for .gitignore files
|
||||||
|
# - cssho.vscode-svgviewer # SVG viewer
|
||||||
|
- davidanson.vscode-markdownlint # Markdown/CommonMark linting and style checking for Visual Studio Code
|
||||||
|
- eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed
|
||||||
|
- EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files
|
||||||
|
- Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar
|
||||||
|
- mechatroner.rainbow-csv # Highlight columns in csv files in different colors
|
||||||
|
# - nextflow.nextflow # Nextflow syntax highlighting
|
||||||
|
- oderwat.indent-rainbow # Highlight indentation level
|
||||||
|
- streetsidesoftware.code-spell-checker # Spelling checker for source code
|
1
.nf-core.yml
Normal file
1
.nf-core.yml
Normal file
|
@ -0,0 +1 @@
|
||||||
|
repository_type: pipeline
|
6
.yamllint.yml
Normal file
6
.yamllint.yml
Normal file
|
@ -0,0 +1,6 @@
|
||||||
|
extends: default
|
||||||
|
|
||||||
|
rules:
|
||||||
|
document-start: disable
|
||||||
|
line-length: disable
|
||||||
|
truthy: disable
|
12
README.md
12
README.md
|
@ -56,22 +56,22 @@ On release, automated continuous integration tests run the pipeline on a full-si
|
||||||
3. Download the pipeline and test it on a minimal dataset with a single command:
|
3. Download the pipeline and test it on a minimal dataset with a single command:
|
||||||
|
|
||||||
```console
|
```console
|
||||||
nextflow run nf-core/taxprofiler -profile test,YOURPROFILE
|
nextflow run nf-core/taxprofiler -profile test,YOURPROFILE --outdir <OUTDIR>
|
||||||
```
|
```
|
||||||
|
|
||||||
Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
|
Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string.
|
||||||
|
|
||||||
> * The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
|
> - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`.
|
||||||
> * Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
|
> - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile <institute>` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment.
|
||||||
> * If you are using `singularity` and are persistently observing issues downloading Singularity images directly due to timeout or network issues, then you can use the `--singularity_pull_docker_container` parameter to pull and convert the Docker image instead. Alternatively, you can use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
|
> - If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs.
|
||||||
> * If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
|
> - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs.
|
||||||
|
|
||||||
4. Start running your own analysis!
|
4. Start running your own analysis!
|
||||||
|
|
||||||
<!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
|
<!-- TODO nf-core: Update the example "typical command" below used to run the pipeline -->
|
||||||
|
|
||||||
```console
|
```console
|
||||||
nextflow run nf-core/taxprofiler -profile <docker/singularity/podman/shifter/charliecloud/conda/institute> --input samplesheet.csv --genome GRCh37
|
nextflow run nf-core/taxprofiler --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile <docker/singularity/podman/shifter/charliecloud/conda/institute>
|
||||||
```
|
```
|
||||||
|
|
||||||
## Documentation
|
## Documentation
|
||||||
|
|
|
@ -37,8 +37,6 @@ def print_error(error, context="Line", context_str=""):
|
||||||
print(error_str)
|
print(error_str)
|
||||||
sys.exit(1)
|
sys.exit(1)
|
||||||
|
|
||||||
|
|
||||||
# TODO nf-core: Update the check_samplesheet function
|
|
||||||
def check_samplesheet(file_in, file_out):
|
def check_samplesheet(file_in, file_out):
|
||||||
"""
|
"""
|
||||||
This function checks that the samplesheet follows the following structure:
|
This function checks that the samplesheet follows the following structure:
|
||||||
|
|
|
@ -1,7 +1,7 @@
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
nf-core/taxprofiler Nextflow base config file
|
nf-core/taxprofiler Nextflow base config file
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
A 'blank slate' config file, appropriate for general use on most high performance
|
A 'blank slate' config file, appropriate for general use on most high performance
|
||||||
compute environments. Assumes that all software is installed and available on
|
compute environments. Assumes that all software is installed and available on
|
||||||
the PATH. Runs in `local` mode - all jobs will be run on the logged in environment.
|
the PATH. Runs in `local` mode - all jobs will be run on the logged in environment.
|
||||||
|
|
|
@ -1,7 +1,7 @@
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Nextflow config file for iGenomes paths
|
Nextflow config file for iGenomes paths
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Defines reference genomes using iGenome paths.
|
Defines reference genomes using iGenome paths.
|
||||||
Can be used by any config that customises the base path using:
|
Can be used by any config that customises the base path using:
|
||||||
$params.igenomes_base / --igenomes_base
|
$params.igenomes_base / --igenomes_base
|
||||||
|
@ -13,7 +13,7 @@ params {
|
||||||
genomes {
|
genomes {
|
||||||
'GRCh37' {
|
'GRCh37' {
|
||||||
fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BismarkIndex/"
|
||||||
|
@ -26,7 +26,7 @@ params {
|
||||||
}
|
}
|
||||||
'GRCh38' {
|
'GRCh38' {
|
||||||
fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BismarkIndex/"
|
||||||
|
@ -38,7 +38,7 @@ params {
|
||||||
}
|
}
|
||||||
'GRCm38' {
|
'GRCm38' {
|
||||||
fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/"
|
||||||
|
@ -51,7 +51,7 @@ params {
|
||||||
}
|
}
|
||||||
'TAIR10' {
|
'TAIR10' {
|
||||||
fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/"
|
||||||
|
@ -62,7 +62,7 @@ params {
|
||||||
}
|
}
|
||||||
'EB2' {
|
'EB2' {
|
||||||
fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/"
|
||||||
|
@ -72,7 +72,7 @@ params {
|
||||||
}
|
}
|
||||||
'UMD3.1' {
|
'UMD3.1' {
|
||||||
fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/"
|
||||||
|
@ -83,7 +83,7 @@ params {
|
||||||
}
|
}
|
||||||
'WBcel235' {
|
'WBcel235' {
|
||||||
fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/"
|
||||||
|
@ -94,7 +94,7 @@ params {
|
||||||
}
|
}
|
||||||
'CanFam3.1' {
|
'CanFam3.1' {
|
||||||
fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/"
|
||||||
|
@ -105,7 +105,7 @@ params {
|
||||||
}
|
}
|
||||||
'GRCz10' {
|
'GRCz10' {
|
||||||
fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/"
|
||||||
|
@ -115,7 +115,7 @@ params {
|
||||||
}
|
}
|
||||||
'BDGP6' {
|
'BDGP6' {
|
||||||
fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/"
|
||||||
|
@ -126,7 +126,7 @@ params {
|
||||||
}
|
}
|
||||||
'EquCab2' {
|
'EquCab2' {
|
||||||
fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/"
|
||||||
|
@ -137,7 +137,7 @@ params {
|
||||||
}
|
}
|
||||||
'EB1' {
|
'EB1' {
|
||||||
fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/"
|
||||||
|
@ -147,7 +147,7 @@ params {
|
||||||
}
|
}
|
||||||
'Galgal4' {
|
'Galgal4' {
|
||||||
fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/"
|
||||||
|
@ -157,7 +157,7 @@ params {
|
||||||
}
|
}
|
||||||
'Gm01' {
|
'Gm01' {
|
||||||
fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/"
|
||||||
|
@ -167,7 +167,7 @@ params {
|
||||||
}
|
}
|
||||||
'Mmul_1' {
|
'Mmul_1' {
|
||||||
fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/"
|
||||||
|
@ -178,7 +178,7 @@ params {
|
||||||
}
|
}
|
||||||
'IRGSP-1.0' {
|
'IRGSP-1.0' {
|
||||||
fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/"
|
||||||
|
@ -188,7 +188,7 @@ params {
|
||||||
}
|
}
|
||||||
'CHIMP2.1.4' {
|
'CHIMP2.1.4' {
|
||||||
fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/"
|
||||||
|
@ -199,7 +199,7 @@ params {
|
||||||
}
|
}
|
||||||
'Rnor_5.0' {
|
'Rnor_5.0' {
|
||||||
fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/"
|
||||||
|
@ -209,7 +209,7 @@ params {
|
||||||
}
|
}
|
||||||
'Rnor_6.0' {
|
'Rnor_6.0' {
|
||||||
fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/"
|
||||||
|
@ -219,7 +219,7 @@ params {
|
||||||
}
|
}
|
||||||
'R64-1-1' {
|
'R64-1-1' {
|
||||||
fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/"
|
||||||
|
@ -230,7 +230,7 @@ params {
|
||||||
}
|
}
|
||||||
'EF2' {
|
'EF2' {
|
||||||
fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/"
|
||||||
|
@ -242,7 +242,7 @@ params {
|
||||||
}
|
}
|
||||||
'Sbi1' {
|
'Sbi1' {
|
||||||
fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/"
|
||||||
|
@ -252,7 +252,7 @@ params {
|
||||||
}
|
}
|
||||||
'Sscrofa10.2' {
|
'Sscrofa10.2' {
|
||||||
fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/"
|
||||||
|
@ -263,7 +263,7 @@ params {
|
||||||
}
|
}
|
||||||
'AGPv3' {
|
'AGPv3' {
|
||||||
fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/"
|
||||||
|
@ -273,7 +273,7 @@ params {
|
||||||
}
|
}
|
||||||
'hg38' {
|
'hg38' {
|
||||||
fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BismarkIndex/"
|
||||||
|
@ -285,7 +285,7 @@ params {
|
||||||
}
|
}
|
||||||
'hg19' {
|
'hg19' {
|
||||||
fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BismarkIndex/"
|
||||||
|
@ -298,7 +298,7 @@ params {
|
||||||
}
|
}
|
||||||
'mm10' {
|
'mm10' {
|
||||||
fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/"
|
||||||
|
@ -311,7 +311,7 @@ params {
|
||||||
}
|
}
|
||||||
'bosTau8' {
|
'bosTau8' {
|
||||||
fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/"
|
||||||
|
@ -321,7 +321,7 @@ params {
|
||||||
}
|
}
|
||||||
'ce10' {
|
'ce10' {
|
||||||
fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/"
|
||||||
|
@ -333,7 +333,7 @@ params {
|
||||||
}
|
}
|
||||||
'canFam3' {
|
'canFam3' {
|
||||||
fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/"
|
||||||
|
@ -344,7 +344,7 @@ params {
|
||||||
}
|
}
|
||||||
'danRer10' {
|
'danRer10' {
|
||||||
fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/"
|
||||||
|
@ -355,7 +355,7 @@ params {
|
||||||
}
|
}
|
||||||
'dm6' {
|
'dm6' {
|
||||||
fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/"
|
||||||
|
@ -366,7 +366,7 @@ params {
|
||||||
}
|
}
|
||||||
'equCab2' {
|
'equCab2' {
|
||||||
fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/"
|
||||||
|
@ -377,7 +377,7 @@ params {
|
||||||
}
|
}
|
||||||
'galGal4' {
|
'galGal4' {
|
||||||
fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/"
|
||||||
|
@ -388,7 +388,7 @@ params {
|
||||||
}
|
}
|
||||||
'panTro4' {
|
'panTro4' {
|
||||||
fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/"
|
||||||
|
@ -399,7 +399,7 @@ params {
|
||||||
}
|
}
|
||||||
'rn6' {
|
'rn6' {
|
||||||
fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/"
|
||||||
|
@ -409,7 +409,7 @@ params {
|
||||||
}
|
}
|
||||||
'sacCer3' {
|
'sacCer3' {
|
||||||
fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/"
|
||||||
|
@ -419,7 +419,7 @@ params {
|
||||||
}
|
}
|
||||||
'susScr3' {
|
'susScr3' {
|
||||||
fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
|
fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa"
|
||||||
bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/genome.fa"
|
bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/"
|
||||||
bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
|
bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/"
|
||||||
star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
|
star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/"
|
||||||
bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
|
bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/"
|
||||||
|
|
|
@ -1,12 +1,12 @@
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Config file for defining DSL2 per module options and publishing paths
|
Config file for defining DSL2 per module options and publishing paths
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Available keys to override module options:
|
Available keys to override module options:
|
||||||
ext.args = Additional arguments appended to command in module.
|
ext.args = Additional arguments appended to command in module.
|
||||||
ext.args2 = Second set of arguments appended to command in module (multi-tool modules).
|
ext.args2 = Second set of arguments appended to command in module (multi-tool modules).
|
||||||
ext.args3 = Third set of arguments appended to command in module (multi-tool modules).
|
ext.args3 = Third set of arguments appended to command in module (multi-tool modules).
|
||||||
ext.prefix = File name prefix for output files.
|
ext.prefix = File name prefix for output files.
|
||||||
----------------------------------------------------------------------------------------
|
----------------------------------------------------------------------------------------
|
||||||
*/
|
*/
|
||||||
|
|
||||||
|
@ -14,14 +14,14 @@ process {
|
||||||
|
|
||||||
publishDir = [
|
publishDir = [
|
||||||
path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
|
path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
|
||||||
mode: 'copy',
|
mode: params.publish_dir_mode,
|
||||||
saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
|
saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
|
||||||
]
|
]
|
||||||
|
|
||||||
withName: SAMPLESHEET_CHECK {
|
withName: SAMPLESHEET_CHECK {
|
||||||
publishDir = [
|
publishDir = [
|
||||||
path: { "${params.outdir}/pipeline_info" },
|
path: { "${params.outdir}/pipeline_info" },
|
||||||
mode: 'copy',
|
mode: params.publish_dir_mode,
|
||||||
saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
|
saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
|
||||||
]
|
]
|
||||||
}
|
}
|
||||||
|
@ -92,7 +92,7 @@ process {
|
||||||
withName: CUSTOM_DUMPSOFTWAREVERSIONS {
|
withName: CUSTOM_DUMPSOFTWAREVERSIONS {
|
||||||
publishDir = [
|
publishDir = [
|
||||||
path: { "${params.outdir}/pipeline_info" },
|
path: { "${params.outdir}/pipeline_info" },
|
||||||
mode: 'copy',
|
mode: params.publish_dir_mode,
|
||||||
pattern: '*_versions.yml'
|
pattern: '*_versions.yml'
|
||||||
]
|
]
|
||||||
}
|
}
|
||||||
|
|
|
@ -1,11 +1,11 @@
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Nextflow config file for running minimal tests
|
Nextflow config file for running minimal tests
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Defines input files and everything required to run a fast and simple pipeline test.
|
Defines input files and everything required to run a fast and simple pipeline test.
|
||||||
|
|
||||||
Use as follows:
|
Use as follows:
|
||||||
nextflow run nf-core/taxprofiler -profile test,<docker/singularity>
|
nextflow run nf-core/taxprofiler -profile test,<docker/singularity> --outdir <OUTDIR>
|
||||||
|
|
||||||
----------------------------------------------------------------------------------------
|
----------------------------------------------------------------------------------------
|
||||||
*/
|
*/
|
||||||
|
|
|
@ -1,11 +1,11 @@
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Nextflow config file for running full-size tests
|
Nextflow config file for running full-size tests
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Defines input files and everything required to run a full size pipeline test.
|
Defines input files and everything required to run a full size pipeline test.
|
||||||
|
|
||||||
Use as follows:
|
Use as follows:
|
||||||
nextflow run nf-core/taxprofiler -profile test_full,<docker/singularity>
|
nextflow run nf-core/taxprofiler -profile test_full,<docker/singularity> --outdir <OUTDIR>
|
||||||
|
|
||||||
----------------------------------------------------------------------------------------
|
----------------------------------------------------------------------------------------
|
||||||
*/
|
*/
|
||||||
|
|
|
@ -57,7 +57,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p
|
||||||
The typical command for running the pipeline is as follows:
|
The typical command for running the pipeline is as follows:
|
||||||
|
|
||||||
```console
|
```console
|
||||||
nextflow run nf-core/taxprofiler --input samplesheet.csv --genome GRCh37 -profile docker
|
nextflow run nf-core/taxprofiler --input samplesheet.csv --outdir <OUTDIR> --genome GRCh37 -profile docker
|
||||||
```
|
```
|
||||||
|
|
||||||
This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
|
This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles.
|
||||||
|
@ -141,11 +141,11 @@ Whilst the default requirements set within the pipeline will hopefully work for
|
||||||
For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue:
|
For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue:
|
||||||
|
|
||||||
```console
|
```console
|
||||||
[62/149eb0] NOTE: Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
|
[62/149eb0] NOTE: Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1)
|
||||||
Error executing process > 'RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
|
Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)'
|
||||||
|
|
||||||
Caused by:
|
Caused by:
|
||||||
Process `RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
|
Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137)
|
||||||
|
|
||||||
Command executed:
|
Command executed:
|
||||||
STAR \
|
STAR \
|
||||||
|
@ -169,17 +169,24 @@ Work dir:
|
||||||
Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
|
Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run`
|
||||||
```
|
```
|
||||||
|
|
||||||
To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so based on the search results the file we want is `modules/nf-core/software/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. Providing you haven't set any other standard nf-core parameters to __cap__ the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
|
To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN).
|
||||||
|
We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/software/star/align/main.nf`.
|
||||||
|
If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9).
|
||||||
|
The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements.
|
||||||
|
The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB.
|
||||||
|
Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB.
|
||||||
|
The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections.
|
||||||
|
|
||||||
```nextflow
|
```nextflow
|
||||||
process {
|
process {
|
||||||
withName: STAR_ALIGN {
|
withName: 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN' {
|
||||||
memory = 100.GB
|
memory = 100.GB
|
||||||
}
|
}
|
||||||
}
|
}
|
||||||
```
|
```
|
||||||
|
|
||||||
> **NB:** We specify just the process name i.e. `STAR_ALIGN` in the config file and not the full task name string that is printed to screen in the error message or on the terminal whilst the pipeline is running i.e. `RNASEQ:ALIGN_STAR:STAR_ALIGN`. You may get a warning suggesting that the process selector isn't recognised but you can ignore that if the process name has been specified correctly. This is something that needs to be fixed upstream in core Nextflow.
|
> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden.
|
||||||
|
> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly.
|
||||||
|
|
||||||
### Updating containers
|
### Updating containers
|
||||||
|
|
||||||
|
|
|
@ -27,7 +27,7 @@ class NfcoreSchema {
|
||||||
/* groovylint-disable-next-line UnusedPrivateMethodParameter */
|
/* groovylint-disable-next-line UnusedPrivateMethodParameter */
|
||||||
public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') {
|
public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') {
|
||||||
def has_error = false
|
def has_error = false
|
||||||
//=====================================================================//
|
//~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
|
||||||
// Check for nextflow core params and unexpected params
|
// Check for nextflow core params and unexpected params
|
||||||
def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text
|
def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text
|
||||||
def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
|
def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions')
|
||||||
|
@ -135,7 +135,7 @@ class NfcoreSchema {
|
||||||
}
|
}
|
||||||
}
|
}
|
||||||
|
|
||||||
//=====================================================================//
|
//~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~//
|
||||||
// Validate parameters against the schema
|
// Validate parameters against the schema
|
||||||
InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream()
|
InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream()
|
||||||
JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream))
|
JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream))
|
||||||
|
|
|
@ -29,12 +29,12 @@ class Utils {
|
||||||
conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults'))
|
conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults'))
|
||||||
|
|
||||||
if (conda_check_failed) {
|
if (conda_check_failed) {
|
||||||
log.warn "=============================================================================\n" +
|
log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
|
||||||
" There is a problem with your Conda configuration!\n\n" +
|
" There is a problem with your Conda configuration!\n\n" +
|
||||||
" You will need to set-up the conda-forge and bioconda channels correctly.\n" +
|
" You will need to set-up the conda-forge and bioconda channels correctly.\n" +
|
||||||
" Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" +
|
" Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" +
|
||||||
" NB: The order of the channels matters!\n" +
|
" NB: The order of the channels matters!\n" +
|
||||||
"==================================================================================="
|
"~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
|
||||||
}
|
}
|
||||||
}
|
}
|
||||||
}
|
}
|
||||||
|
|
|
@ -49,11 +49,11 @@ class WorkflowTaxprofiler {
|
||||||
//
|
//
|
||||||
private static void genomeExistsError(params, log) {
|
private static void genomeExistsError(params, log) {
|
||||||
if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
|
if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) {
|
||||||
log.error "=============================================================================\n" +
|
log.error "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" +
|
||||||
" Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
|
" Genome '${params.genome}' not found in any config files provided to the pipeline.\n" +
|
||||||
" Currently, the available genome keys are:\n" +
|
" Currently, the available genome keys are:\n" +
|
||||||
" ${params.genomes.keySet().join(", ")}\n" +
|
" ${params.genomes.keySet().join(", ")}\n" +
|
||||||
"==================================================================================="
|
"~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~"
|
||||||
System.exit(1)
|
System.exit(1)
|
||||||
}
|
}
|
||||||
}
|
}
|
||||||
|
|
24
main.nf
24
main.nf
|
@ -1,8 +1,8 @@
|
||||||
#!/usr/bin/env nextflow
|
#!/usr/bin/env nextflow
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
nf-core/taxprofiler
|
nf-core/taxprofiler
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Github : https://github.com/nf-core/taxprofiler
|
Github : https://github.com/nf-core/taxprofiler
|
||||||
Website: https://nf-co.re/taxprofiler
|
Website: https://nf-co.re/taxprofiler
|
||||||
Slack : https://nfcore.slack.com/channels/taxprofiler
|
Slack : https://nfcore.slack.com/channels/taxprofiler
|
||||||
|
@ -12,25 +12,25 @@
|
||||||
nextflow.enable.dsl = 2
|
nextflow.enable.dsl = 2
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
GENOME PARAMETER VALUES
|
GENOME PARAMETER VALUES
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
|
params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta')
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
VALIDATE & PRINT PARAMETER SUMMARY
|
VALIDATE & PRINT PARAMETER SUMMARY
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
WorkflowMain.initialise(workflow, params, log)
|
WorkflowMain.initialise(workflow, params, log)
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
NAMED WORKFLOW FOR PIPELINE
|
NAMED WORKFLOW FOR PIPELINE
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
include { TAXPROFILER } from './workflows/taxprofiler'
|
include { TAXPROFILER } from './workflows/taxprofiler'
|
||||||
|
@ -43,9 +43,9 @@ workflow NFCORE_TAXPROFILER {
|
||||||
}
|
}
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
RUN ALL WORKFLOWS
|
RUN ALL WORKFLOWS
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
//
|
//
|
||||||
|
@ -57,7 +57,7 @@ workflow {
|
||||||
}
|
}
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
THE END
|
THE END
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
|
@ -1,7 +1,7 @@
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
nf-core/taxprofiler Nextflow config file
|
nf-core/taxprofiler Nextflow config file
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
Default config options for all compute environments
|
Default config options for all compute environments
|
||||||
----------------------------------------------------------------------------------------
|
----------------------------------------------------------------------------------------
|
||||||
*/
|
*/
|
||||||
|
@ -24,8 +24,9 @@ params {
|
||||||
max_multiqc_email_size = '25.MB'
|
max_multiqc_email_size = '25.MB'
|
||||||
|
|
||||||
// Boilerplate options
|
// Boilerplate options
|
||||||
outdir = './results'
|
outdir = null
|
||||||
tracedir = "${params.outdir}/pipeline_info"
|
tracedir = "${params.outdir}/pipeline_info"
|
||||||
|
publish_dir_mode = 'copy'
|
||||||
email = null
|
email = null
|
||||||
email_on_fail = null
|
email_on_fail = null
|
||||||
plaintext_email = false
|
plaintext_email = false
|
||||||
|
@ -75,6 +76,15 @@ try {
|
||||||
System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
|
System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config")
|
||||||
}
|
}
|
||||||
|
|
||||||
|
// Load nf-core/taxprofiler custom profiles from different institutions.
|
||||||
|
// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs!
|
||||||
|
// try {
|
||||||
|
// includeConfig "${params.custom_config_base}/pipeline/taxprofiler.config"
|
||||||
|
// } catch (Exception e) {
|
||||||
|
// System.err.println("WARNING: Could not load nf-core/config/taxprofiler profiles: ${params.custom_config_base}/pipeline/taxprofiler.config")
|
||||||
|
// }
|
||||||
|
|
||||||
|
|
||||||
profiles {
|
profiles {
|
||||||
debug { process.beforeScript = 'echo $HOSTNAME' }
|
debug { process.beforeScript = 'echo $HOSTNAME' }
|
||||||
conda {
|
conda {
|
||||||
|
|
|
@ -11,7 +11,8 @@
|
||||||
"fa_icon": "fas fa-terminal",
|
"fa_icon": "fas fa-terminal",
|
||||||
"description": "Define where the pipeline should find input data and save output data.",
|
"description": "Define where the pipeline should find input data and save output data.",
|
||||||
"required": [
|
"required": [
|
||||||
"input"
|
"input",
|
||||||
|
"outdir"
|
||||||
],
|
],
|
||||||
"properties": {
|
"properties": {
|
||||||
"input": {
|
"input": {
|
||||||
|
@ -26,8 +27,8 @@
|
||||||
},
|
},
|
||||||
"outdir": {
|
"outdir": {
|
||||||
"type": "string",
|
"type": "string",
|
||||||
"description": "Path to the output directory where the results will be saved.",
|
"format": "directory-path",
|
||||||
"default": "./results",
|
"description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.",
|
||||||
"fa_icon": "fas fa-folder-open"
|
"fa_icon": "fas fa-folder-open"
|
||||||
},
|
},
|
||||||
"email": {
|
"email": {
|
||||||
|
@ -169,6 +170,22 @@
|
||||||
"fa_icon": "fas fa-question-circle",
|
"fa_icon": "fas fa-question-circle",
|
||||||
"hidden": true
|
"hidden": true
|
||||||
},
|
},
|
||||||
|
"publish_dir_mode": {
|
||||||
|
"type": "string",
|
||||||
|
"default": "copy",
|
||||||
|
"description": "Method used to save pipeline results to output directory.",
|
||||||
|
"help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.",
|
||||||
|
"fa_icon": "fas fa-copy",
|
||||||
|
"enum": [
|
||||||
|
"symlink",
|
||||||
|
"rellink",
|
||||||
|
"link",
|
||||||
|
"copy",
|
||||||
|
"copyNoFollow",
|
||||||
|
"move"
|
||||||
|
],
|
||||||
|
"hidden": true
|
||||||
|
},
|
||||||
"email_on_fail": {
|
"email_on_fail": {
|
||||||
"type": "string",
|
"type": "string",
|
||||||
"description": "Email address for completion summary, only when pipeline fails.",
|
"description": "Email address for completion summary, only when pipeline fails.",
|
||||||
|
|
|
@ -35,26 +35,28 @@ workflow INPUT_CHECK {
|
||||||
}
|
}
|
||||||
|
|
||||||
// Function to get list of [ meta, [ fastq_1, fastq_2 ] ]
|
// Function to get list of [ meta, [ fastq_1, fastq_2 ] ]
|
||||||
def create_fastq_channels(LinkedHashMap row) {
|
def create_fastq_channel(LinkedHashMap row) {
|
||||||
|
// create meta map
|
||||||
def meta = [:]
|
def meta = [:]
|
||||||
meta.id = row.sample
|
meta.id = row.sample
|
||||||
meta.run_accession = row.run_accession
|
meta.run_accession = row.run_accession
|
||||||
meta.instrument_platform = row.instrument_platform
|
meta.instrument_platform = row.instrument_platform
|
||||||
meta.single_end = row.single_end.toBoolean()
|
meta.single_end = row.single_end.toBoolean()
|
||||||
|
|
||||||
def array = []
|
// add path(s) of the fastq file(s) to the meta map
|
||||||
|
def fastq_meta = []
|
||||||
if (!file(row.fastq_1).exists()) {
|
if (!file(row.fastq_1).exists()) {
|
||||||
exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}"
|
exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}"
|
||||||
}
|
}
|
||||||
if (meta.single_end) {
|
if (meta.single_end) {
|
||||||
array = [ meta, [ file(row.fastq_1) ] ]
|
fastq_meta = [ meta, [ file(row.fastq_1) ] ]
|
||||||
} else {
|
} else {
|
||||||
if (!file(row.fastq_2).exists()) {
|
if (!file(row.fastq_2).exists()) {
|
||||||
exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}"
|
exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}"
|
||||||
}
|
}
|
||||||
array = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ]
|
fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ]
|
||||||
}
|
}
|
||||||
return array
|
return fastq_meta
|
||||||
}
|
}
|
||||||
|
|
||||||
// Function to get list of [ meta, fasta ]
|
// Function to get list of [ meta, fasta ]
|
||||||
|
|
|
@ -1,7 +1,7 @@
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
VALIDATE INPUTS
|
VALIDATE INPUTS
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
|
def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params)
|
||||||
|
@ -19,18 +19,18 @@ if (params.input ) { ch_input = file(params.input) } else { exit 1, '
|
||||||
if (params.databases) { ch_databases = file(params.databases) } else { exit 1, 'Input database sheet not specified!' }
|
if (params.databases) { ch_databases = file(params.databases) } else { exit 1, 'Input database sheet not specified!' }
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
CONFIG FILES
|
CONFIG FILES
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true)
|
ch_multiqc_config = file("$projectDir/assets/multiqc_config.yaml", checkIfExists: true)
|
||||||
ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
|
ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty()
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
IMPORT LOCAL MODULES/SUBWORKFLOWS
|
IMPORT LOCAL MODULES/SUBWORKFLOWS
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
//
|
//
|
||||||
|
@ -43,9 +43,9 @@ include { FASTQ_PREPROCESSING } from '../subworkflows/local/preprocessing'
|
||||||
|
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
IMPORT NF-CORE MODULES/SUBWORKFLOWS
|
IMPORT NF-CORE MODULES/SUBWORKFLOWS
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
//
|
//
|
||||||
|
@ -61,9 +61,9 @@ include { KRAKEN2_KRAKEN2 } from '../modules/nf-core/modules/kraken2
|
||||||
|
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
RUN MAIN WORKFLOW
|
RUN MAIN WORKFLOW
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
// Info required for completion email and summary
|
// Info required for completion email and summary
|
||||||
|
@ -213,9 +213,9 @@ workflow TAXPROFILER {
|
||||||
}
|
}
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
COMPLETION EMAIL AND SUMMARY
|
COMPLETION EMAIL AND SUMMARY
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
||||||
workflow.onComplete {
|
workflow.onComplete {
|
||||||
|
@ -226,7 +226,7 @@ workflow.onComplete {
|
||||||
}
|
}
|
||||||
|
|
||||||
/*
|
/*
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
THE END
|
THE END
|
||||||
========================================================================================
|
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
|
||||||
*/
|
*/
|
||||||
|
|
Loading…
Reference in a new issue