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Start works - need updated module though t have meta
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parent
3fc7f1f0f8
commit
a764bc4807
5 changed files with 100 additions and 3 deletions
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@ -327,7 +327,7 @@ process {
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]
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}
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withName: METAPHLAN3 {
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withName: METAPHLAN3_METAPHLAN3 {
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ext.args = { "${meta.db_params}" }
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ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" }
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publishDir = [
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@ -337,6 +337,15 @@ process {
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]
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}
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withName: METAPHLAN3_MERGEMETAPHLANTABLES {
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ext.prefix = { "${meta.id}_combined_reports" }
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publishDir = [
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path: { "${params.outdir}/metaphlan3/" },
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mode: params.publish_dir_mode,
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pattern: '*.{txt}'
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]
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}
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withName: CENTRIFUGE_CENTRIFUGE {
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publishDir = [
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path: { "${params.outdir}/centrifuge/${meta.db_name}/" },
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@ -94,6 +94,10 @@
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"branch": "master",
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"git_sha": "2d38566eca4cc15142b2ffa7c11837569b39aece"
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},
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"metaphlan3/mergemetaphlantables": {
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"branch": "master",
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"git_sha": "940d7fe9d63962e0e2ba0987e2893fb0aff832e3"
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},
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"metaphlan3/metaphlan3": {
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"branch": "master",
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"git_sha": "940d7fe9d63962e0e2ba0987e2893fb0aff832e3"
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33
modules/nf-core/modules/metaphlan3/mergemetaphlantables/main.nf
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33
modules/nf-core/modules/metaphlan3/mergemetaphlantables/main.nf
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@ -0,0 +1,33 @@
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process METAPHLAN3_MERGEMETAPHLANTABLES {
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label 'process_single'
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conda (params.enable_conda ? 'bioconda::metaphlan=3.0.12' : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/metaphlan:3.0.12--pyhb7b1952_0' :
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'quay.io/biocontainers/metaphlan:3.0.12--pyhb7b1952_0' }"
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input:
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path(profiles)
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output:
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tuple path("${prefix}.txt") , emit: txt
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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prefix = task.ext.prefix ?: "merged_abundance_table"
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"""
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merge_metaphlan_tables.py \\
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$args \\
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-o ${prefix}.txt \\
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${profiles}
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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metaphlan3: \$(metaphlan --version 2>&1 | awk '{print \$3}')
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END_VERSIONS
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"""
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}
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34
modules/nf-core/modules/metaphlan3/mergemetaphlantables/meta.yml
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34
modules/nf-core/modules/metaphlan3/mergemetaphlantables/meta.yml
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@ -0,0 +1,34 @@
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name: "metaphlan3_mergemetaphlantables"
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description: Merges output abundance tables from MetaPhlAn3
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keywords:
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- metagenomics
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- classification
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- merge
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- table
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- profiles
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tools:
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- metaphlan3:
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description: Identify clades (phyla to species) present in the metagenome obtained from a microbiome sample and their relative abundance
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homepage: https://huttenhower.sph.harvard.edu/metaphlan/
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documentation: https://github.com/biobakery/MetaPhlAn
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doi: "10.7554/eLife.65088"
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licence: ["MIT License"]
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input:
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- profiles:
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type: file
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description: List of per-sample MetaPhlAn3 taxonomic abundance tables
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pattern: "*"
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output:
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- versions:
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type: file
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description: File containing software versions
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pattern: "versions.yml"
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- txt:
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type: txt
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description: Combined MetaPhlAn3 table
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pattern: "*.txt"
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authors:
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- "@jfy133"
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@ -2,8 +2,9 @@
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// Standardise output files e.g. aggregation
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//
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include { KAIJU_KAIJU2TABLE } from '../../modules/nf-core/modules/kaiju/kaiju2table/main'
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include { MOTUS_MERGE } from '../../modules/nf-core/modules/motus/merge/main'
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include { KAIJU_KAIJU2TABLE } from '../../modules/nf-core/modules/kaiju/kaiju2table/main'
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include { MOTUS_MERGE } from '../../modules/nf-core/modules/motus/merge/main'
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include { METAPHLAN3_MERGEMETAPHLANTABLES } from '../../modules/nf-core/modules/metaphlan3/mergemetaphlantables/main'
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workflow STANDARDISATION_PROFILES {
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take:
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@ -23,6 +24,7 @@ workflow STANDARDISATION_PROFILES {
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ch_input_profiles = profiles
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.branch {
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motus: it[0]['tool'] == 'motus'
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metaphlan3: it[0]['tool'] == 'metaphlan3'
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unknown: true
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}
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@ -58,6 +60,21 @@ workflow STANDARDISATION_PROFILES {
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ch_multiqc_files = ch_multiqc_files.mix( KAIJU_KAIJU2TABLE.out.summary )
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ch_versions = ch_versions.mix( KAIJU_KAIJU2TABLE.out.versions )
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// MetaPhlAn3
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ch_profiles_for_metaphlan3 = ch_input_profiles.metaphlan3
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.map { [it[0]['db_name'], it[1]] }
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.groupTuple()
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.map {
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[it[1]]
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}
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METAPHLAN3_MERGEMETAPHLANTABLES ( ch_profiles_for_metaphlan3 )
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ch_standardised_tables = ch_standardised_tables.mix( METAPHLAN3_MERGEMETAPHLANTABLES.out.txt )
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ch_multiqc_files = ch_multiqc_files.mix( METAPHLAN3_MERGEMETAPHLANTABLES.out.txt )
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ch_versions = ch_versions.mix( METAPHLAN3_MERGEMETAPHLANTABLES.out.versions )
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// mOTUs
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// mOTUs has a 'single' database, and cannot create custom ones.
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// Therefore removing db info here, and publish merged at root mOTUs results
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// directory
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